Ruminococcaceae bacterium P7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2881 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G4WUZ3|A0A1G4WUZ3_9FIRM Uncharacterized protein OS=Ruminococcaceae bacterium P7 OX=1200751 GN=SAMN02910436_02551 PE=4 SV=1
MM1 pKa = 7.21NANGEE6 pKa = 4.23FTKK9 pKa = 10.99NDD11 pKa = 3.6LAVDD15 pKa = 3.72RR16 pKa = 11.84DD17 pKa = 3.76MEE19 pKa = 4.55IDD21 pKa = 3.63CDD23 pKa = 3.44IGQEE27 pKa = 3.68ILVYY31 pKa = 8.41FEE33 pKa = 4.97CWFDD37 pKa = 3.57ADD39 pKa = 4.18RR40 pKa = 11.84KK41 pKa = 10.38FGIDD45 pKa = 3.23TSEE48 pKa = 3.6NDD50 pKa = 2.97AWLNMYY56 pKa = 9.95GRR58 pKa = 11.84YY59 pKa = 9.36NPYY62 pKa = 10.54EE63 pKa = 4.06DD64 pKa = 3.94TLTIEE69 pKa = 4.48CEE71 pKa = 3.58IDD73 pKa = 3.51RR74 pKa = 11.84DD75 pKa = 4.14DD76 pKa = 4.15GSSYY80 pKa = 11.07FDD82 pKa = 3.9YY83 pKa = 10.94FPTDD87 pKa = 3.32AEE89 pKa = 4.31SQLMKK94 pKa = 10.87DD95 pKa = 4.67LITAKK100 pKa = 10.03IQEE103 pKa = 4.96EE104 pKa = 4.56YY105 pKa = 10.42HH106 pKa = 4.65QTPKK110 pKa = 10.48EE111 pKa = 4.13FCEE114 pKa = 4.35STFDD118 pKa = 4.34DD119 pKa = 4.56SIEE122 pKa = 3.97IGGII126 pKa = 3.46

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G4WQT9|A0A1G4WQT9_9FIRM Uncharacterized protein OS=Ruminococcaceae bacterium P7 OX=1200751 GN=SAMN02910436_02165 PE=4 SV=1
MM1 pKa = 7.95ASITKK6 pKa = 10.32RR7 pKa = 11.84KK8 pKa = 9.81DD9 pKa = 3.16SYY11 pKa = 11.12FVAVSCGYY19 pKa = 9.83DD20 pKa = 3.08QNGRR24 pKa = 11.84QIRR27 pKa = 11.84RR28 pKa = 11.84TMTYY32 pKa = 10.04KK33 pKa = 10.71PEE35 pKa = 4.77ANMTEE40 pKa = 3.87RR41 pKa = 11.84QIQKK45 pKa = 9.44EE46 pKa = 4.41VQRR49 pKa = 11.84QAVLFEE55 pKa = 4.02EE56 pKa = 4.79QVRR59 pKa = 11.84NGQVSSDD66 pKa = 3.56GRR68 pKa = 11.84LKK70 pKa = 10.95LADD73 pKa = 4.06FVPSISRR80 pKa = 11.84PPSNVCRR87 pKa = 11.84PMFTSATRR95 pKa = 11.84ILSISALFRR104 pKa = 11.84RR105 pKa = 11.84SGII108 pKa = 3.67

Molecular weight: 12.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2881

0

2881

890244

39

3456

309.0

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.987 ± 0.051

1.626 ± 0.021

6.098 ± 0.039

6.944 ± 0.046

4.355 ± 0.033

6.788 ± 0.046

1.679 ± 0.02

6.916 ± 0.039

6.567 ± 0.044

8.627 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.022

4.725 ± 0.044

3.426 ± 0.03

3.009 ± 0.022

4.682 ± 0.044

6.242 ± 0.041

5.828 ± 0.054

6.806 ± 0.039

0.856 ± 0.017

4.189 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski