Amino acid dipepetide frequency for Ruminococcaceae bacterium P7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.472AlaAla: 8.472 ± 0.14
1.177AlaCys: 1.177 ± 0.04
5.278AlaAsp: 5.278 ± 0.075
6.28AlaGlu: 6.28 ± 0.09
3.559AlaPhe: 3.559 ± 0.07
5.2AlaGly: 5.2 ± 0.105
1.212AlaHis: 1.212 ± 0.036
4.941AlaIle: 4.941 ± 0.083
4.913AlaLys: 4.913 ± 0.08
7.434AlaLeu: 7.434 ± 0.102
2.095AlaMet: 2.095 ± 0.049
2.908AlaAsn: 2.908 ± 0.065
2.543AlaPro: 2.543 ± 0.079
2.785AlaGln: 2.785 ± 0.068
3.061AlaArg: 3.061 ± 0.061
3.948AlaSer: 3.948 ± 0.078
3.692AlaThr: 3.692 ± 0.081
7.064AlaVal: 7.064 ± 0.103
0.563AlaTrp: 0.563 ± 0.028
2.745AlaTyr: 2.745 ± 0.053
0.001AlaXaa: 0.001 ± 0.001
Cys
1.331CysAla: 1.331 ± 0.046
0.329CysCys: 0.329 ± 0.019
1.031CysAsp: 1.031 ± 0.033
1.108CysGlu: 1.108 ± 0.037
0.732CysPhe: 0.732 ± 0.028
1.81CysGly: 1.81 ± 0.055
0.308CysHis: 0.308 ± 0.019
0.949CysIle: 0.949 ± 0.037
0.878CysLys: 0.878 ± 0.029
1.132CysLeu: 1.132 ± 0.038
0.409CysMet: 0.409 ± 0.023
0.592CysAsn: 0.592 ± 0.026
0.646CysPro: 0.646 ± 0.033
0.388CysGln: 0.388 ± 0.023
0.905CysArg: 0.905 ± 0.031
1.033CysSer: 1.033 ± 0.033
0.851CysThr: 0.851 ± 0.035
1.082CysVal: 1.082 ± 0.034
0.12CysTrp: 0.12 ± 0.011
0.623CysTyr: 0.623 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
4.805AspAla: 4.805 ± 0.086
1.057AspCys: 1.057 ± 0.033
3.836AspAsp: 3.836 ± 0.086
4.912AspGlu: 4.912 ± 0.071
3.089AspPhe: 3.089 ± 0.059
5.028AspGly: 5.028 ± 0.094
0.705AspHis: 0.705 ± 0.03
4.676AspIle: 4.676 ± 0.064
3.852AspLys: 3.852 ± 0.069
4.439AspLeu: 4.439 ± 0.077
1.657AspMet: 1.657 ± 0.039
3.004AspAsn: 3.004 ± 0.063
1.606AspPro: 1.606 ± 0.046
0.894AspGln: 0.894 ± 0.034
2.513AspArg: 2.513 ± 0.061
3.824AspSer: 3.824 ± 0.064
3.393AspThr: 3.393 ± 0.065
3.93AspVal: 3.93 ± 0.066
0.605AspTrp: 0.605 ± 0.03
3.149AspTyr: 3.149 ± 0.063
0.0AspXaa: 0.0 ± 0.0
Glu
4.904GluAla: 4.904 ± 0.076
0.836GluCys: 0.836 ± 0.031
3.751GluAsp: 3.751 ± 0.07
5.291GluGlu: 5.291 ± 0.091
2.505GluPhe: 2.505 ± 0.061
3.741GluGly: 3.741 ± 0.076
1.237GluHis: 1.237 ± 0.042
5.288GluIle: 5.288 ± 0.08
5.939GluLys: 5.939 ± 0.093
6.426GluLeu: 6.426 ± 0.102
2.141GluMet: 2.141 ± 0.053
4.329GluAsn: 4.329 ± 0.075
1.99GluPro: 1.99 ± 0.081
2.768GluGln: 2.768 ± 0.068
3.371GluArg: 3.371 ± 0.077
3.691GluSer: 3.691 ± 0.072
3.635GluThr: 3.635 ± 0.068
3.93GluVal: 3.93 ± 0.068
0.638GluTrp: 0.638 ± 0.026
3.033GluTyr: 3.033 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.383PheAla: 3.383 ± 0.059
0.937PheCys: 0.937 ± 0.035
3.225PheAsp: 3.225 ± 0.066
2.937PheGlu: 2.937 ± 0.058
2.063PhePhe: 2.063 ± 0.061
3.18PheGly: 3.18 ± 0.072
0.732PheHis: 0.732 ± 0.027
2.922PheIle: 2.922 ± 0.057
2.113PheLys: 2.113 ± 0.055
3.532PheLeu: 3.532 ± 0.068
1.021PheMet: 1.021 ± 0.032
1.961PheAsn: 1.961 ± 0.046
1.409PhePro: 1.409 ± 0.042
1.068PheGln: 1.068 ± 0.035
2.01PheArg: 2.01 ± 0.047
3.253PheSer: 3.253 ± 0.059
2.698PheThr: 2.698 ± 0.065
2.891PheVal: 2.891 ± 0.062
0.4PheTrp: 0.4 ± 0.021
1.813PheTyr: 1.813 ± 0.047
0.0PheXaa: 0.0 ± 0.0
Gly
5.094GlyAla: 5.094 ± 0.1
1.36GlyCys: 1.36 ± 0.035
3.907GlyAsp: 3.907 ± 0.086
4.684GlyGlu: 4.684 ± 0.067
3.143GlyPhe: 3.143 ± 0.074
4.876GlyGly: 4.876 ± 0.114
1.012GlyHis: 1.012 ± 0.033
5.048GlyIle: 5.048 ± 0.08
5.309GlyLys: 5.309 ± 0.084
5.146GlyLeu: 5.146 ± 0.096
2.047GlyMet: 2.047 ± 0.053
3.15GlyAsn: 3.15 ± 0.067
0.901GlyPro: 0.901 ± 0.038
1.72GlyGln: 1.72 ± 0.047
2.871GlyArg: 2.871 ± 0.056
4.273GlySer: 4.273 ± 0.08
4.198GlyThr: 4.198 ± 0.094
5.221GlyVal: 5.221 ± 0.086
0.726GlyTrp: 0.726 ± 0.038
3.192GlyTyr: 3.192 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
1.177HisAla: 1.177 ± 0.041
0.325HisCys: 0.325 ± 0.019
0.914HisAsp: 0.914 ± 0.031
0.946HisGlu: 0.946 ± 0.035
0.887HisPhe: 0.887 ± 0.034
1.261HisGly: 1.261 ± 0.04
0.447HisHis: 0.447 ± 0.048
1.313HisIle: 1.313 ± 0.041
0.942HisLys: 0.942 ± 0.035
1.355HisLeu: 1.355 ± 0.039
0.371HisMet: 0.371 ± 0.019
0.804HisAsn: 0.804 ± 0.029
0.859HisPro: 0.859 ± 0.033
0.439HisGln: 0.439 ± 0.022
0.89HisArg: 0.89 ± 0.036
1.047HisSer: 1.047 ± 0.033
1.015HisThr: 1.015 ± 0.036
0.876HisVal: 0.876 ± 0.03
0.156HisTrp: 0.156 ± 0.014
0.759HisTyr: 0.759 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.977IleAla: 5.977 ± 0.091
1.228IleCys: 1.228 ± 0.037
4.479IleAsp: 4.479 ± 0.078
4.435IleGlu: 4.435 ± 0.078
2.719IlePhe: 2.719 ± 0.064
4.674IleGly: 4.674 ± 0.081
1.217IleHis: 1.217 ± 0.039
4.959IleIle: 4.959 ± 0.099
4.158IleLys: 4.158 ± 0.074
5.688IleLeu: 5.688 ± 0.093
1.624IleMet: 1.624 ± 0.048
3.191IleAsn: 3.191 ± 0.065
2.933IlePro: 2.933 ± 0.062
1.893IleGln: 1.893 ± 0.043
3.514IleArg: 3.514 ± 0.075
4.766IleSer: 4.766 ± 0.091
4.184IleThr: 4.184 ± 0.073
4.49IleVal: 4.49 ± 0.078
0.53IleTrp: 0.53 ± 0.024
2.497IleTyr: 2.497 ± 0.055
0.0IleXaa: 0.0 ± 0.0
Lys
5.22LysAla: 5.22 ± 0.08
0.766LysCys: 0.766 ± 0.03
3.565LysAsp: 3.565 ± 0.072
4.827LysGlu: 4.827 ± 0.086
1.895LysPhe: 1.895 ± 0.047
3.988LysGly: 3.988 ± 0.073
1.062LysHis: 1.062 ± 0.036
4.4LysIle: 4.4 ± 0.067
5.469LysLys: 5.469 ± 0.096
5.724LysLeu: 5.724 ± 0.087
1.851LysMet: 1.851 ± 0.044
3.562LysAsn: 3.562 ± 0.074
2.479LysPro: 2.479 ± 0.062
2.224LysGln: 2.224 ± 0.05
3.515LysArg: 3.515 ± 0.079
3.83LysSer: 3.83 ± 0.073
4.175LysThr: 4.175 ± 0.079
3.788LysVal: 3.788 ± 0.071
0.568LysTrp: 0.568 ± 0.025
2.763LysTyr: 2.763 ± 0.065
0.0LysXaa: 0.0 ± 0.0
Leu
6.558LeuAla: 6.558 ± 0.09
1.706LeuCys: 1.706 ± 0.057
5.124LeuAsp: 5.124 ± 0.082
4.977LeuGlu: 4.977 ± 0.086
3.954LeuPhe: 3.954 ± 0.08
5.411LeuGly: 5.411 ± 0.088
1.576LeuHis: 1.576 ± 0.047
5.685LeuIle: 5.685 ± 0.102
5.365LeuLys: 5.365 ± 0.079
7.983LeuLeu: 7.983 ± 0.123
2.347LeuMet: 2.347 ± 0.057
4.057LeuAsn: 4.057 ± 0.072
3.529LeuPro: 3.529 ± 0.067
2.435LeuGln: 2.435 ± 0.054
4.507LeuArg: 4.507 ± 0.094
6.708LeuSer: 6.708 ± 0.101
5.492LeuThr: 5.492 ± 0.086
4.771LeuVal: 4.771 ± 0.078
0.718LeuTrp: 0.718 ± 0.031
3.369LeuTyr: 3.369 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
1.833MetAla: 1.833 ± 0.045
0.361MetCys: 0.361 ± 0.019
1.398MetAsp: 1.398 ± 0.04
1.601MetGlu: 1.601 ± 0.044
1.024MetPhe: 1.024 ± 0.037
1.577MetGly: 1.577 ± 0.047
0.394MetHis: 0.394 ± 0.02
1.839MetIle: 1.839 ± 0.045
2.241MetLys: 2.241 ± 0.054
2.749MetLeu: 2.749 ± 0.061
0.804MetMet: 0.804 ± 0.032
1.404MetAsn: 1.404 ± 0.042
1.111MetPro: 1.111 ± 0.048
0.915MetGln: 0.915 ± 0.034
1.261MetArg: 1.261 ± 0.036
1.659MetSer: 1.659 ± 0.039
1.749MetThr: 1.749 ± 0.04
1.538MetVal: 1.538 ± 0.044
0.182MetTrp: 0.182 ± 0.016
0.874MetTyr: 0.874 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
4.035AsnAla: 4.035 ± 0.082
0.742AsnCys: 0.742 ± 0.03
2.628AsnAsp: 2.628 ± 0.06
3.086AsnGlu: 3.086 ± 0.047
1.679AsnPhe: 1.679 ± 0.042
4.081AsnGly: 4.081 ± 0.088
0.865AsnHis: 0.865 ± 0.032
3.387AsnIle: 3.387 ± 0.068
2.688AsnLys: 2.688 ± 0.07
3.593AsnLeu: 3.593 ± 0.063
1.213AsnMet: 1.213 ± 0.033
2.358AsnAsn: 2.358 ± 0.066
2.248AsnPro: 2.248 ± 0.047
1.405AsnGln: 1.405 ± 0.044
2.329AsnArg: 2.329 ± 0.05
2.84AsnSer: 2.84 ± 0.064
2.784AsnThr: 2.784 ± 0.067
2.913AsnVal: 2.913 ± 0.06
0.418AsnTrp: 0.418 ± 0.023
1.961AsnTyr: 1.961 ± 0.05
0.0AsnXaa: 0.0 ± 0.0
Pro
2.687ProAla: 2.687 ± 0.077
0.491ProCys: 0.491 ± 0.018
2.669ProAsp: 2.669 ± 0.066
3.023ProGlu: 3.023 ± 0.065
1.639ProPhe: 1.639 ± 0.043
1.653ProGly: 1.653 ± 0.054
0.618ProHis: 0.618 ± 0.026
1.971ProIle: 1.971 ± 0.051
2.167ProLys: 2.167 ± 0.049
2.81ProLeu: 2.81 ± 0.057
0.816ProMet: 0.816 ± 0.034
1.503ProAsn: 1.503 ± 0.045
0.953ProPro: 0.953 ± 0.033
1.304ProGln: 1.304 ± 0.047
1.11ProArg: 1.11 ± 0.035
1.93ProSer: 1.93 ± 0.052
2.163ProThr: 2.163 ± 0.11
2.978ProVal: 2.978 ± 0.065
0.286ProTrp: 0.286 ± 0.019
1.486ProTyr: 1.486 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
2.349GlnAla: 2.349 ± 0.058
0.349GlnCys: 0.349 ± 0.02
1.246GlnAsp: 1.246 ± 0.036
1.755GlnGlu: 1.755 ± 0.049
1.178GlnPhe: 1.178 ± 0.036
1.687GlnGly: 1.687 ± 0.049
0.458GlnHis: 0.458 ± 0.024
2.211GlnIle: 2.211 ± 0.061
2.347GlnLys: 2.347 ± 0.057
2.853GlnLeu: 2.853 ± 0.074
0.892GlnMet: 0.892 ± 0.034
1.731GlnAsn: 1.731 ± 0.046
1.192GlnPro: 1.192 ± 0.049
1.27GlnGln: 1.27 ± 0.05
1.53GlnArg: 1.53 ± 0.041
1.984GlnSer: 1.984 ± 0.045
1.877GlnThr: 1.877 ± 0.042
1.512GlnVal: 1.512 ± 0.038
0.259GlnTrp: 0.259 ± 0.017
1.407GlnTyr: 1.407 ± 0.048
0.0GlnXaa: 0.0 ± 0.0
Arg
3.08ArgAla: 3.08 ± 0.055
0.717ArgCys: 0.717 ± 0.034
2.585ArgAsp: 2.585 ± 0.063
3.87ArgGlu: 3.87 ± 0.084
2.347ArgPhe: 2.347 ± 0.049
2.642ArgGly: 2.642 ± 0.063
0.933ArgHis: 0.933 ± 0.033
3.416ArgIle: 3.416 ± 0.074
3.353ArgLys: 3.353 ± 0.067
4.338ArgLeu: 4.338 ± 0.08
1.312ArgMet: 1.312 ± 0.038
2.19ArgAsn: 2.19 ± 0.043
1.345ArgPro: 1.345 ± 0.045
1.6ArgGln: 1.6 ± 0.044
2.628ArgArg: 2.628 ± 0.062
2.478ArgSer: 2.478 ± 0.055
2.304ArgThr: 2.304 ± 0.05
3.112ArgVal: 3.112 ± 0.065
0.408ArgTrp: 0.408 ± 0.021
2.16ArgTyr: 2.16 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
5.144SerAla: 5.144 ± 0.082
0.951SerCys: 0.951 ± 0.031
4.183SerAsp: 4.183 ± 0.071
4.359SerGlu: 4.359 ± 0.076
3.009SerPhe: 3.009 ± 0.063
5.35SerGly: 5.35 ± 0.104
0.99SerHis: 0.99 ± 0.03
3.73SerIle: 3.73 ± 0.071
3.637SerLys: 3.637 ± 0.063
5.34SerLeu: 5.34 ± 0.075
1.565SerMet: 1.565 ± 0.037
2.448SerAsn: 2.448 ± 0.073
1.917SerPro: 1.917 ± 0.051
1.774SerGln: 1.774 ± 0.046
2.889SerArg: 2.889 ± 0.067
3.92SerSer: 3.92 ± 0.094
3.022SerThr: 3.022 ± 0.071
5.063SerVal: 5.063 ± 0.09
0.491SerTrp: 0.491 ± 0.026
2.634SerTyr: 2.634 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
5.524ThrAla: 5.524 ± 0.098
0.684ThrCys: 0.684 ± 0.029
3.999ThrAsp: 3.999 ± 0.082
4.21ThrGlu: 4.21 ± 0.084
2.351ThrPhe: 2.351 ± 0.063
4.472ThrGly: 4.472 ± 0.087
0.939ThrHis: 0.939 ± 0.032
3.848ThrIle: 3.848 ± 0.067
3.082ThrLys: 3.082 ± 0.072
5.075ThrLeu: 5.075 ± 0.073
1.327ThrMet: 1.327 ± 0.044
2.214ThrAsn: 2.214 ± 0.06
2.341ThrPro: 2.341 ± 0.044
1.686ThrGln: 1.686 ± 0.05
2.046ThrArg: 2.046 ± 0.046
3.069ThrSer: 3.069 ± 0.075
3.398ThrThr: 3.398 ± 0.126
5.35ThrVal: 5.35 ± 0.116
0.432ThrTrp: 0.432 ± 0.023
2.23ThrTyr: 2.23 ± 0.057
0.0ThrXaa: 0.0 ± 0.0
Val
4.726ValAla: 4.726 ± 0.08
1.274ValCys: 1.274 ± 0.044
3.76ValAsp: 3.76 ± 0.065
4.022ValGlu: 4.022 ± 0.076
3.351ValPhe: 3.351 ± 0.071
3.876ValGly: 3.876 ± 0.074
1.039ValHis: 1.039 ± 0.036
5.158ValIle: 5.158 ± 0.089
4.436ValLys: 4.436 ± 0.07
6.21ValLeu: 6.21 ± 0.09
1.858ValMet: 1.858 ± 0.045
3.281ValAsn: 3.281 ± 0.064
2.633ValPro: 2.633 ± 0.065
1.802ValGln: 1.802 ± 0.046
3.267ValArg: 3.267 ± 0.07
4.886ValSer: 4.886 ± 0.075
4.655ValThr: 4.655 ± 0.111
4.493ValVal: 4.493 ± 0.079
0.598ValTrp: 0.598 ± 0.025
2.735ValTyr: 2.735 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.547TrpAla: 0.547 ± 0.023
0.14TrpCys: 0.14 ± 0.013
0.511TrpAsp: 0.511 ± 0.029
0.534TrpGlu: 0.534 ± 0.024
0.399TrpPhe: 0.399 ± 0.022
0.584TrpGly: 0.584 ± 0.029
0.19TrpHis: 0.19 ± 0.015
0.558TrpIle: 0.558 ± 0.03
0.602TrpLys: 0.602 ± 0.029
0.86TrpLeu: 0.86 ± 0.037
0.245TrpMet: 0.245 ± 0.015
0.532TrpAsn: 0.532 ± 0.029
0.155TrpPro: 0.155 ± 0.016
0.37TrpGln: 0.37 ± 0.023
0.375TrpArg: 0.375 ± 0.021
0.502TrpSer: 0.502 ± 0.027
0.464TrpThr: 0.464 ± 0.028
0.466TrpVal: 0.466 ± 0.023
0.111TrpTrp: 0.111 ± 0.01
0.412TrpTyr: 0.412 ± 0.025
0.001TrpXaa: 0.001 ± 0.001
Tyr
3.026TyrAla: 3.026 ± 0.057
0.828TyrCys: 0.828 ± 0.034
2.886TyrAsp: 2.886 ± 0.064
2.587TyrGlu: 2.587 ± 0.054
2.024TyrPhe: 2.024 ± 0.054
2.786TyrGly: 2.786 ± 0.06
0.851TyrHis: 0.851 ± 0.035
2.855TyrIle: 2.855 ± 0.054
2.18TyrLys: 2.18 ± 0.055
3.583TyrLeu: 3.583 ± 0.074
0.921TyrMet: 0.921 ± 0.038
2.031TyrAsn: 2.031 ± 0.056
1.467TyrPro: 1.467 ± 0.041
1.348TyrGln: 1.348 ± 0.049
2.227TyrArg: 2.227 ± 0.056
2.775TyrSer: 2.775 ± 0.063
2.527TyrThr: 2.527 ± 0.073
2.591TyrVal: 2.591 ± 0.051
0.349TyrTrp: 0.349 ± 0.019
2.044TyrTyr: 2.044 ± 0.056
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.001XaaGlu: 0.001 ± 0.001
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.001XaaAsn: 0.001 ± 0.001
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.007XaaXaa: 0.007 ± 0.004
Statistics based on 2881 proteins (890244 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski