Proteome-pI 2.0 - Proteome Isoelectric Point Database

Hubei diptera virus 16

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.27

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1L3KG09|A0A1L3KG09_9VIRU RNA-directed RNA polymerase OS=Hubei diptera virus 16 OX=1922877 PE=3 SV=1

MM1 pKa = 7.67FSQRR5 pKa = 11.84KK6 pKa = 8.6VNSLCGSLQNAAVRR20 pKa = 11.84YY21 pKa = 7.56KK22 pKa = 10.26WSVAGLTAAYY32 pKa = 9.68VAYY35 pKa = 10.19QLAKK39 pKa = 10.16RR40 pKa = 11.84SHH42 pKa = 6.78LLCSVLSKK50 pKa = 10.97NINQGVRR57 pKa = 11.84EE58 pKa = 3.97KK59 pKa = 10.94FNAEE63 pKa = 3.6FHH65 pKa = 6.03VQATAPRR72 pKa = 11.84DD73 pKa = 3.61EE74 pKa = 4.62SHH76 pKa = 6.47SHH78 pKa = 6.73PEE80 pKa = 3.72SARR83 pKa = 11.84NRR85 pKa = 11.84TDD87 pKa = 2.88AEE89 pKa = 3.97YY90 pKa = 10.79SIDD93 pKa = 3.55LFARR97 pKa = 11.84SIGLEE102 pKa = 4.06PYY104 pKa = 10.27AVSTSARR111 pKa = 11.84NTHH114 pKa = 6.67DD115 pKa = 3.43GQRR118 pKa = 11.84LYY120 pKa = 10.05YY121 pKa = 8.13TAKK124 pKa = 10.52DD125 pKa = 2.79ACLPYY130 pKa = 10.55KK131 pKa = 10.24CDD133 pKa = 3.49PVRR136 pKa = 11.84DD137 pKa = 3.79YY138 pKa = 11.66HH139 pKa = 7.71LLTFVDD145 pKa = 3.13VDD147 pKa = 3.97YY148 pKa = 11.07YY149 pKa = 11.82ADD151 pKa = 3.46MAKK154 pKa = 9.85WLWTGRR160 pKa = 11.84PALVYY165 pKa = 7.05TFCPTSAGGVIPEE178 pKa = 4.45GTFHH182 pKa = 6.76IQDD185 pKa = 3.92DD186 pKa = 4.43EE187 pKa = 5.06LFVVYY192 pKa = 10.54SGGARR197 pKa = 11.84YY198 pKa = 8.15NHH200 pKa = 6.7KK201 pKa = 10.45LWDD204 pKa = 3.86YY205 pKa = 11.16NVDD208 pKa = 3.41HH209 pKa = 6.71LVVKK213 pKa = 10.7GRR215 pKa = 11.84LFSLLVKK222 pKa = 10.03VDD224 pKa = 3.83QVVLPSDD231 pKa = 3.37KK232 pKa = 10.13QRR234 pKa = 11.84RR235 pKa = 11.84IVLLTPTTWYY245 pKa = 9.69PSMYY249 pKa = 10.22NGLLRR254 pKa = 11.84TRR256 pKa = 11.84NEE258 pKa = 3.12LRR260 pKa = 11.84RR261 pKa = 11.84RR262 pKa = 11.84KK263 pKa = 9.67FSYY266 pKa = 10.91GPVNVLTYY274 pKa = 10.11IDD276 pKa = 3.55RR277 pKa = 11.84TRR279 pKa = 11.84DD280 pKa = 3.08GEE282 pKa = 4.16AAKK285 pKa = 9.77TNLMVSVSLAGCPAHH300 pKa = 6.57ATVDD304 pKa = 3.73YY305 pKa = 9.05ATFCAIMARR314 pKa = 11.84FLHH317 pKa = 5.81TKK319 pKa = 9.44NPNIADD325 pKa = 3.57IEE327 pKa = 4.36RR328 pKa = 11.84MLRR331 pKa = 11.84AKK333 pKa = 10.32GVEE336 pKa = 4.54DD337 pKa = 4.03AASMAPIIYY346 pKa = 8.1EE347 pKa = 3.59AWNNGWCKK355 pKa = 10.51RR356 pKa = 11.84NNDD359 pKa = 3.82EE360 pKa = 4.27KK361 pKa = 11.2QVGAGEE367 pKa = 4.27ARR369 pKa = 11.84GKK371 pKa = 10.48APHH374 pKa = 6.19FQHH377 pKa = 6.88IGPLAWEE384 pKa = 4.8DD385 pKa = 4.12GEE387 pKa = 5.28DD388 pKa = 3.23IGVQVFDD395 pKa = 5.6PIVTGAGIFPASSEE409 pKa = 4.28NNDD412 pKa = 2.88TAAIVGRR419 pKa = 11.84VEE421 pKa = 3.99NVRR424 pKa = 11.84NTVNPGGKK432 pKa = 8.31YY433 pKa = 9.2RR434 pKa = 11.84KK435 pKa = 9.59YY436 pKa = 9.19FNEE439 pKa = 5.09FIRR442 pKa = 11.84AFPIGVLEE450 pKa = 4.93PYY452 pKa = 11.13SMDD455 pKa = 3.72YY456 pKa = 10.93VIEE459 pKa = 4.22KK460 pKa = 10.1QNRR463 pKa = 11.84PLQKK467 pKa = 10.07VRR469 pKa = 11.84CQKK472 pKa = 10.39HH473 pKa = 5.78KK474 pKa = 11.13NLIGQAITVVRR485 pKa = 11.84SFMKK489 pKa = 10.33RR490 pKa = 11.84EE491 pKa = 4.02AYY493 pKa = 10.16GKK495 pKa = 8.47ITDD498 pKa = 3.98PRR500 pKa = 11.84NISTLPPSHH509 pKa = 6.19TMEE512 pKa = 4.55LSAYY516 pKa = 7.98TYY518 pKa = 10.2AAKK521 pKa = 10.71EE522 pKa = 4.0EE523 pKa = 4.51VFRR526 pKa = 11.84TMPWFMPCKK535 pKa = 9.69NPRR538 pKa = 11.84EE539 pKa = 3.72ISAAVMEE546 pKa = 4.58LAEE549 pKa = 4.13KK550 pKa = 10.41HH551 pKa = 6.15KK552 pKa = 10.74EE553 pKa = 3.96ILSTDD558 pKa = 3.5YY559 pKa = 11.4SRR561 pKa = 11.84LDD563 pKa = 3.2GTLSYY568 pKa = 10.78FLRR571 pKa = 11.84TVEE574 pKa = 3.81HH575 pKa = 6.4AVYY578 pKa = 10.36KK579 pKa = 10.7RR580 pKa = 11.84LFNPKK585 pKa = 9.33YY586 pKa = 10.08RR587 pKa = 11.84PRR589 pKa = 11.84LLKK592 pKa = 10.95LLDD595 pKa = 3.91SEE597 pKa = 4.72MTCPAFTDD605 pKa = 3.26TGLKK609 pKa = 9.34YY610 pKa = 8.93QQKK613 pKa = 9.91SSRR616 pKa = 11.84LSGSPTTTDD625 pKa = 2.93GNTLIVAFVLYY636 pKa = 10.34CAARR640 pKa = 11.84NARR643 pKa = 11.84MIKK646 pKa = 9.89PGEE649 pKa = 3.96IMNIPGLVYY658 pKa = 10.7GDD660 pKa = 4.16DD661 pKa = 3.95TLCGGLRR668 pKa = 11.84ASVMEE673 pKa = 4.08RR674 pKa = 11.84TARR677 pKa = 11.84DD678 pKa = 2.95LGLTLKK684 pKa = 11.03CEE686 pKa = 4.23VSKK689 pKa = 10.84EE690 pKa = 4.12CQPVTFLSRR699 pKa = 11.84VFVDD703 pKa = 3.8PWTTSTSVQEE713 pKa = 4.37PKK715 pKa = 10.22RR716 pKa = 11.84ALGKK720 pKa = 8.64MHH722 pKa = 7.19LSVIRR727 pKa = 11.84NIDD730 pKa = 3.07KK731 pKa = 10.9RR732 pKa = 11.84LVARR736 pKa = 11.84NKK738 pKa = 10.5AKK740 pKa = 10.63GYY742 pKa = 10.11LATDD746 pKa = 3.27KK747 pKa = 9.58LTPLMSEE754 pKa = 4.4YY755 pKa = 9.8ATHH758 pKa = 7.1ILEE761 pKa = 4.35KK762 pKa = 10.31TSMFTDD768 pKa = 4.06DD769 pKa = 5.33MMTKK773 pKa = 10.47YY774 pKa = 10.96DD775 pKa = 3.73FTRR778 pKa = 11.84DD779 pKa = 3.29TNFWVYY785 pKa = 10.76EE786 pKa = 4.01YY787 pKa = 11.0GEE789 pKa = 4.17NEE791 pKa = 3.92AWPQTLCDD799 pKa = 4.16LEE801 pKa = 5.18LMEE804 pKa = 5.34SVVAKK809 pKa = 10.64HH810 pKa = 6.62LDD812 pKa = 3.43LTVDD816 pKa = 3.59QLRR819 pKa = 11.84TKK821 pKa = 10.48CDD823 pKa = 3.12EE824 pKa = 4.31VKK826 pKa = 10.52RR827 pKa = 11.84GNWTPIHH834 pKa = 6.97FDD836 pKa = 3.23MEE838 pKa = 4.4VPPNVIVRR846 pKa = 11.84HH847 pKa = 5.26EE848 pKa = 4.18PRR850 pKa = 11.84PEE852 pKa = 3.7QQQSGRR858 pKa = 11.84RR859 pKa = 11.84RR860 pKa = 11.84NRR862 pKa = 11.84TQRR865 pKa = 11.84SVV867 pKa = 2.68

Molecular weight: 98.58 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1L3KG09|A0A1L3KG09_9VIRU RNA-directed RNA polymerase OS=Hubei diptera virus 16 OX=1922877 PE=3 SV=1

MM1 pKa = 7.67FSQRR5 pKa = 11.84KK6 pKa = 8.6VNSLCGSLQNAAVRR20 pKa = 11.84YY21 pKa = 7.56KK22 pKa = 10.26WSVAGLTAAYY32 pKa = 9.68VAYY35 pKa = 10.19QLAKK39 pKa = 10.16RR40 pKa = 11.84SHH42 pKa = 6.78LLCSVLSKK50 pKa = 10.97NINQGVRR57 pKa = 11.84EE58 pKa = 3.97KK59 pKa = 10.94FNAEE63 pKa = 3.6FHH65 pKa = 6.03VQATAPRR72 pKa = 11.84DD73 pKa = 3.61EE74 pKa = 4.62SHH76 pKa = 6.47SHH78 pKa = 6.73PEE80 pKa = 3.72SARR83 pKa = 11.84NRR85 pKa = 11.84TDD87 pKa = 2.88AEE89 pKa = 3.97YY90 pKa = 10.79SIDD93 pKa = 3.55LFARR97 pKa = 11.84SIGLEE102 pKa = 4.06PYY104 pKa = 10.27AVSTSARR111 pKa = 11.84NTHH114 pKa = 6.67DD115 pKa = 3.43GQRR118 pKa = 11.84LYY120 pKa = 10.05YY121 pKa = 8.13TAKK124 pKa = 10.52DD125 pKa = 2.79ACLPYY130 pKa = 10.55KK131 pKa = 10.24CDD133 pKa = 3.49PVRR136 pKa = 11.84DD137 pKa = 3.79YY138 pKa = 11.66HH139 pKa = 7.71LLTFVDD145 pKa = 3.13VDD147 pKa = 3.97YY148 pKa = 11.07YY149 pKa = 11.82ADD151 pKa = 3.46MAKK154 pKa = 9.85WLWTGRR160 pKa = 11.84PALVYY165 pKa = 7.05TFCPTSAGGVIPEE178 pKa = 4.45GTFHH182 pKa = 6.76IQDD185 pKa = 3.92DD186 pKa = 4.43EE187 pKa = 5.06LFVVYY192 pKa = 10.54SGGARR197 pKa = 11.84YY198 pKa = 8.15NHH200 pKa = 6.7KK201 pKa = 10.45LWDD204 pKa = 3.86YY205 pKa = 11.16NVDD208 pKa = 3.41HH209 pKa = 6.71LVVKK213 pKa = 10.7GRR215 pKa = 11.84LFSLLVKK222 pKa = 10.03VDD224 pKa = 3.83QVVLPSDD231 pKa = 3.37KK232 pKa = 10.13QRR234 pKa = 11.84RR235 pKa = 11.84IVLLTPTTWYY245 pKa = 9.69PSMYY249 pKa = 10.22NGLLRR254 pKa = 11.84TRR256 pKa = 11.84NEE258 pKa = 3.12LRR260 pKa = 11.84RR261 pKa = 11.84RR262 pKa = 11.84KK263 pKa = 9.67FSYY266 pKa = 10.91GPVNVLTYY274 pKa = 10.11IDD276 pKa = 3.55RR277 pKa = 11.84TRR279 pKa = 11.84DD280 pKa = 3.08GEE282 pKa = 4.16AAKK285 pKa = 9.77TNLMVSVSLAGCPAHH300 pKa = 6.57ATVDD304 pKa = 3.73YY305 pKa = 9.05ATFCAIMARR314 pKa = 11.84FLHH317 pKa = 5.81TKK319 pKa = 9.44NPNIADD325 pKa = 3.57IEE327 pKa = 4.36RR328 pKa = 11.84MLRR331 pKa = 11.84AKK333 pKa = 10.32GVEE336 pKa = 4.54DD337 pKa = 4.03AASMAPIIYY346 pKa = 8.1EE347 pKa = 3.59AWNNGWCKK355 pKa = 10.51RR356 pKa = 11.84NNDD359 pKa = 3.82EE360 pKa = 4.27KK361 pKa = 11.2QVGAGEE367 pKa = 4.27ARR369 pKa = 11.84GKK371 pKa = 10.48APHH374 pKa = 6.19FQHH377 pKa = 6.88IGPLAWEE384 pKa = 4.8DD385 pKa = 4.12GEE387 pKa = 5.28DD388 pKa = 3.23IGVQVFDD395 pKa = 5.6PIVTGAGIFPASSEE409 pKa = 4.28NNDD412 pKa = 2.88TAAIVGRR419 pKa = 11.84VEE421 pKa = 3.99NVRR424 pKa = 11.84NTVNPGGKK432 pKa = 8.31YY433 pKa = 9.2RR434 pKa = 11.84KK435 pKa = 9.59YY436 pKa = 9.19FNEE439 pKa = 5.09FIRR442 pKa = 11.84AFPIGVLEE450 pKa = 4.93PYY452 pKa = 11.13SMDD455 pKa = 3.72YY456 pKa = 10.93VIEE459 pKa = 4.22KK460 pKa = 10.1QNRR463 pKa = 11.84PLQKK467 pKa = 10.07VRR469 pKa = 11.84CQKK472 pKa = 10.39HH473 pKa = 5.78KK474 pKa = 11.13NLIGQAITVVRR485 pKa = 11.84SFMKK489 pKa = 10.33RR490 pKa = 11.84EE491 pKa = 4.02AYY493 pKa = 10.16GKK495 pKa = 8.47ITDD498 pKa = 3.98PRR500 pKa = 11.84NISTLPPSHH509 pKa = 6.19TMEE512 pKa = 4.55LSAYY516 pKa = 7.98TYY518 pKa = 10.2AAKK521 pKa = 10.71EE522 pKa = 4.0EE523 pKa = 4.51VFRR526 pKa = 11.84TMPWFMPCKK535 pKa = 9.69NPRR538 pKa = 11.84EE539 pKa = 3.72ISAAVMEE546 pKa = 4.58LAEE549 pKa = 4.13KK550 pKa = 10.41HH551 pKa = 6.15KK552 pKa = 10.74EE553 pKa = 3.96ILSTDD558 pKa = 3.5YY559 pKa = 11.4SRR561 pKa = 11.84LDD563 pKa = 3.2GTLSYY568 pKa = 10.78FLRR571 pKa = 11.84TVEE574 pKa = 3.81HH575 pKa = 6.4AVYY578 pKa = 10.36KK579 pKa = 10.7RR580 pKa = 11.84LFNPKK585 pKa = 9.33YY586 pKa = 10.08RR587 pKa = 11.84PRR589 pKa = 11.84LLKK592 pKa = 10.95LLDD595 pKa = 3.91SEE597 pKa = 4.72MTCPAFTDD605 pKa = 3.26TGLKK609 pKa = 9.34YY610 pKa = 8.93QQKK613 pKa = 9.91SSRR616 pKa = 11.84LSGSPTTTDD625 pKa = 2.93GNTLIVAFVLYY636 pKa = 10.34CAARR640 pKa = 11.84NARR643 pKa = 11.84MIKK646 pKa = 9.89PGEE649 pKa = 3.96IMNIPGLVYY658 pKa = 10.7GDD660 pKa = 4.16DD661 pKa = 3.95TLCGGLRR668 pKa = 11.84ASVMEE673 pKa = 4.08RR674 pKa = 11.84TARR677 pKa = 11.84DD678 pKa = 2.95LGLTLKK684 pKa = 11.03CEE686 pKa = 4.23VSKK689 pKa = 10.84EE690 pKa = 4.12CQPVTFLSRR699 pKa = 11.84VFVDD703 pKa = 3.8PWTTSTSVQEE713 pKa = 4.37PKK715 pKa = 10.22RR716 pKa = 11.84ALGKK720 pKa = 8.64MHH722 pKa = 7.19LSVIRR727 pKa = 11.84NIDD730 pKa = 3.07KK731 pKa = 10.9RR732 pKa = 11.84LVARR736 pKa = 11.84NKK738 pKa = 10.5AKK740 pKa = 10.63GYY742 pKa = 10.11LATDD746 pKa = 3.27KK747 pKa = 9.58LTPLMSEE754 pKa = 4.4YY755 pKa = 9.8ATHH758 pKa = 7.1ILEE761 pKa = 4.35KK762 pKa = 10.31TSMFTDD768 pKa = 4.06DD769 pKa = 5.33MMTKK773 pKa = 10.47YY774 pKa = 10.96DD775 pKa = 3.73FTRR778 pKa = 11.84DD779 pKa = 3.29TNFWVYY785 pKa = 10.76EE786 pKa = 4.01YY787 pKa = 11.0GEE789 pKa = 4.17NEE791 pKa = 3.92AWPQTLCDD799 pKa = 4.16LEE801 pKa = 5.18LMEE804 pKa = 5.34SVVAKK809 pKa = 10.64HH810 pKa = 6.62LDD812 pKa = 3.43LTVDD816 pKa = 3.59QLRR819 pKa = 11.84TKK821 pKa = 10.48CDD823 pKa = 3.12EE824 pKa = 4.31VKK826 pKa = 10.52RR827 pKa = 11.84GNWTPIHH834 pKa = 6.97FDD836 pKa = 3.23MEE838 pKa = 4.4VPPNVIVRR846 pKa = 11.84HH847 pKa = 5.26EE848 pKa = 4.18PRR850 pKa = 11.84PEE852 pKa = 3.7QQQSGRR858 pKa = 11.84RR859 pKa = 11.84RR860 pKa = 11.84NRR862 pKa = 11.84TQRR865 pKa = 11.84SVV867 pKa = 2.68

Molecular weight: 98.58 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	867	867	867	867.0	98.58

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
7.843 ± 0.0	1.961 ± 0.0	5.536 ± 0.0	5.536 ± 0.0	3.576 ± 0.0	5.421 ± 0.0	2.537 ± 0.0	4.037 ± 0.0	5.767 ± 0.0	8.304 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.768 ± 0.0	4.614 ± 0.0	5.19 ± 0.0	2.999 ± 0.0	7.497 ± 0.0	5.767 ± 0.0	6.92 ± 0.0	7.612 ± 0.0	1.499 ± 0.0	4.614 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski