Lake Sinai Virus SA1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes; Nodamuvirales; Sinhaliviridae; Sinaivirus; unclassified Sinaivirus

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6R6J6|A0A1W6R6J6_9VIRU RNA-directed RNA polymerase OS=Lake Sinai Virus SA1 OX=1983562 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.08VAFIISIALFGVCQVPPVSHH22 pKa = 7.17WIEE25 pKa = 4.14DD26 pKa = 3.83VIDD29 pKa = 4.75EE30 pKa = 4.55YY31 pKa = 11.73NTMCSYY37 pKa = 10.94EE38 pKa = 4.01YY39 pKa = 10.91AAASAYY45 pKa = 10.56NDD47 pKa = 2.97FVYY50 pKa = 10.22RR51 pKa = 11.84HH52 pKa = 5.75RR53 pKa = 11.84VAAFAAGVRR62 pKa = 11.84VHH64 pKa = 7.41KK65 pKa = 10.78YY66 pKa = 8.79RR67 pKa = 11.84APWYY71 pKa = 8.99CFASRR76 pKa = 11.84PVPIVHH82 pKa = 6.79PLSWLMNQYY91 pKa = 10.38EE92 pKa = 4.51STVDD96 pKa = 3.3RR97 pKa = 11.84VGDD100 pKa = 3.51LLGRR104 pKa = 11.84LEE106 pKa = 4.14IANVSITDD114 pKa = 3.66GLRR117 pKa = 11.84TVADD121 pKa = 3.77TAVRR125 pKa = 11.84AYY127 pKa = 9.49MYY129 pKa = 10.0YY130 pKa = 10.09QIIWIQLTSIVVLIGCFSAIICSSRR155 pKa = 11.84VRR157 pKa = 11.84IVRR160 pKa = 11.84IRR162 pKa = 11.84NRR164 pKa = 11.84VRR166 pKa = 11.84GYY168 pKa = 10.37SISEE172 pKa = 3.75LRR174 pKa = 11.84SEE176 pKa = 4.67FEE178 pKa = 3.79EE179 pKa = 4.52AMGSILAPVSRR190 pKa = 11.84GHH192 pKa = 6.4SCLNFQRR199 pKa = 11.84RR200 pKa = 11.84VVEE203 pKa = 4.36SWAIDD208 pKa = 3.18QLLRR212 pKa = 11.84YY213 pKa = 8.99FRR215 pKa = 11.84RR216 pKa = 11.84LRR218 pKa = 11.84LLSSSPGRR226 pKa = 11.84WNDD229 pKa = 2.67IAHH232 pKa = 7.04RR233 pKa = 11.84LHH235 pKa = 6.64RR236 pKa = 11.84CAPVVLDD243 pKa = 3.7GSFVPEE249 pKa = 3.55CDD251 pKa = 3.17QRR253 pKa = 11.84NNNCCRR259 pKa = 11.84HH260 pKa = 6.04PSVCPEE266 pKa = 3.95RR267 pKa = 11.84FEE269 pKa = 5.49LPASLISHH277 pKa = 6.19VDD279 pKa = 3.38YY280 pKa = 11.2YY281 pKa = 11.76LSLDD285 pKa = 3.35QLAAVVTGPTFIVNHH300 pKa = 7.21DD301 pKa = 3.74YY302 pKa = 11.41SAVDD306 pKa = 3.45TLSVAEE312 pKa = 4.08ASSRR316 pKa = 11.84VAGGLVTTSVKK327 pKa = 10.69DD328 pKa = 3.63GPTYY332 pKa = 10.61GPHH335 pKa = 7.37PYY337 pKa = 10.05YY338 pKa = 10.21HH339 pKa = 7.11WSDD342 pKa = 3.34EE343 pKa = 4.29GVVVASTGAFKK354 pKa = 10.84YY355 pKa = 10.42YY356 pKa = 10.15RR357 pKa = 11.84VGRR360 pKa = 11.84LFDD363 pKa = 3.32STLYY367 pKa = 10.65YY368 pKa = 10.85AFPASGTYY376 pKa = 10.18SHH378 pKa = 8.06DD379 pKa = 3.49DD380 pKa = 3.22PAALRR385 pKa = 11.84RR386 pKa = 11.84STSGDD391 pKa = 2.95QHH393 pKa = 7.05YY394 pKa = 10.68YY395 pKa = 10.14SPHH398 pKa = 5.73EE399 pKa = 4.13KK400 pKa = 10.41RR401 pKa = 11.84FVSYY405 pKa = 8.9TADD408 pKa = 3.12ATHH411 pKa = 5.66YY412 pKa = 9.97HH413 pKa = 5.43VFGVAVPRR421 pKa = 11.84SLADD425 pKa = 3.09YY426 pKa = 10.3CAATFCRR433 pKa = 11.84SVRR436 pKa = 11.84DD437 pKa = 3.51DD438 pKa = 3.47RR439 pKa = 11.84FYY441 pKa = 11.63DD442 pKa = 3.53SLRR445 pKa = 11.84SYY447 pKa = 9.17YY448 pKa = 9.02QNRR451 pKa = 11.84CRR453 pKa = 11.84AIGFSDD459 pKa = 4.19ARR461 pKa = 11.84DD462 pKa = 3.46TLMLDD467 pKa = 4.82FIIHH471 pKa = 6.59LCDD474 pKa = 3.9DD475 pKa = 3.77ASLRR479 pKa = 11.84TFGFSRR485 pKa = 11.84LSAAPSSWTAYY496 pKa = 8.96CLSWVLVKK504 pKa = 10.51VNHH507 pKa = 5.73MMPLALTSYY516 pKa = 11.14VLDD519 pKa = 3.93ALHH522 pKa = 7.04RR523 pKa = 11.84FFGSKK528 pKa = 9.44AAPWNWATIHH538 pKa = 6.56LPTYY542 pKa = 11.42DD543 pKa = 3.23MVTSPFRR550 pKa = 11.84LRR552 pKa = 11.84LFGRR556 pKa = 11.84NPTVFNLEE564 pKa = 3.89RR565 pKa = 11.84FRR567 pKa = 11.84AEE569 pKa = 3.81ATVVGAPDD577 pKa = 4.71LGQSPEE583 pKa = 4.24GASEE587 pKa = 3.71NHH589 pKa = 6.0NEE591 pKa = 3.68YY592 pKa = 10.47DD593 pKa = 3.35IQSGYY598 pKa = 11.0SSAAPCTTPSPLLSYY613 pKa = 10.59SSSEE617 pKa = 4.04GSSLLLDD624 pKa = 4.24DD625 pKa = 5.32VSDD628 pKa = 4.21LVPEE632 pKa = 4.27YY633 pKa = 10.76VVPTSTTSDD642 pKa = 2.67RR643 pKa = 11.84GAGVRR648 pKa = 11.84PNKK651 pKa = 10.28SKK653 pKa = 11.01AGRR656 pKa = 11.84KK657 pKa = 7.68SRR659 pKa = 11.84RR660 pKa = 11.84SANRR664 pKa = 11.84DD665 pKa = 3.43SITDD669 pKa = 3.55NEE671 pKa = 4.35NDD673 pKa = 3.47FPHH676 pKa = 7.29PKK678 pKa = 8.64RR679 pKa = 11.84TTYY682 pKa = 10.79NLCPDD687 pKa = 4.05PTASCGPHH695 pKa = 6.48FFMSVCEE702 pKa = 4.07QNEE705 pKa = 4.54SIPTLFHH712 pKa = 5.75AHH714 pKa = 6.14AVGGEE719 pKa = 4.37DD720 pKa = 2.82ITHH723 pKa = 5.76VVDD726 pKa = 3.55PTLGEE731 pKa = 4.34TISKK735 pKa = 10.12RR736 pKa = 11.84FSASQLRR743 pKa = 11.84LLGWSIDD750 pKa = 4.21GIFSTLSGAATSSFVEE766 pKa = 4.3STLLSLSRR774 pKa = 11.84FMRR777 pKa = 11.84EE778 pKa = 3.53IPTTQPVTEE787 pKa = 4.35ARR789 pKa = 11.84RR790 pKa = 11.84SLLFCGPRR798 pKa = 11.84EE799 pKa = 4.01RR800 pKa = 11.84IKK802 pKa = 11.06GYY804 pKa = 8.65DD805 pKa = 3.12TFDD808 pKa = 3.0IGFMGIAVPSGKK820 pKa = 8.6TKK822 pKa = 10.45GVEE825 pKa = 3.51ACLRR829 pKa = 11.84EE830 pKa = 4.25VAGQHH835 pKa = 5.87ARR837 pKa = 11.84ADD839 pKa = 3.75SPYY842 pKa = 10.42EE843 pKa = 4.08SPP845 pKa = 4.79

Molecular weight: 94.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6R6J6|A0A1W6R6J6_9VIRU RNA-directed RNA polymerase OS=Lake Sinai Virus SA1 OX=1983562 PE=4 SV=1
MM1 pKa = 7.3GAIPLSLTWSDD12 pKa = 3.56FVLRR16 pKa = 11.84PRR18 pKa = 11.84LLVPPILASLPRR30 pKa = 11.84VPAKK34 pKa = 10.08TITNMISSLVTRR46 pKa = 11.84VLRR49 pKa = 11.84PVPLPHH55 pKa = 7.35PYY57 pKa = 10.44LVTPLRR63 pKa = 11.84RR64 pKa = 11.84DD65 pKa = 3.01RR66 pKa = 11.84AYY68 pKa = 11.31YY69 pKa = 9.19STTSPTSFLSMLSPRR84 pKa = 11.84AQRR87 pKa = 11.84RR88 pKa = 11.84TEE90 pKa = 3.69VLEE93 pKa = 4.03YY94 pKa = 11.0VLTKK98 pKa = 10.52AKK100 pKa = 10.39QGASPVDD107 pKa = 4.29LLTVTPLLTTKK118 pKa = 8.0TTFLIQRR125 pKa = 11.84EE126 pKa = 4.4PLTTSAPTQLPRR138 pKa = 11.84ADD140 pKa = 5.1LISSCLCVSRR150 pKa = 11.84MSRR153 pKa = 11.84YY154 pKa = 9.59LLSSMHH160 pKa = 5.85TRR162 pKa = 11.84WEE164 pKa = 4.02VRR166 pKa = 11.84ISPTLLIQLWEE177 pKa = 3.95RR178 pKa = 11.84LYY180 pKa = 11.54LNGLVLRR187 pKa = 11.84NYY189 pKa = 10.31ASSAGLSTEE198 pKa = 3.91YY199 pKa = 10.61LALYY203 pKa = 8.01LAPPPHH209 pKa = 6.88RR210 pKa = 11.84LSSQRR215 pKa = 11.84CCPYY219 pKa = 10.41HH220 pKa = 7.04DD221 pKa = 4.88LCAKK225 pKa = 9.85YY226 pKa = 10.18LQLSPLQKK234 pKa = 10.24LAAVFYY240 pKa = 10.87SVALEE245 pKa = 4.34KK246 pKa = 10.38EE247 pKa = 4.58LKK249 pKa = 9.61DD250 pKa = 3.73TTPLTLDD257 pKa = 3.48SWVSRR262 pKa = 11.84YY263 pKa = 9.22PLARR267 pKa = 11.84QKK269 pKa = 10.0EE270 pKa = 3.94LRR272 pKa = 11.84LAYY275 pKa = 9.67EE276 pKa = 4.26RR277 pKa = 11.84LRR279 pKa = 11.84GSTLVQTAHH288 pKa = 5.39TKK290 pKa = 9.48VRR292 pKa = 11.84NFIKK296 pKa = 10.49VEE298 pKa = 3.91PMSKK302 pKa = 10.44CSDD305 pKa = 3.34PRR307 pKa = 11.84NISPRR312 pKa = 11.84NDD314 pKa = 2.99ATLSTLGPYY323 pKa = 10.4FSAIEE328 pKa = 3.99HH329 pKa = 6.27SAASLPFLIKK339 pKa = 10.79GCDD342 pKa = 2.94IPNRR346 pKa = 11.84SRR348 pKa = 11.84KK349 pKa = 8.86MSNLLGWSNYY359 pKa = 8.53YY360 pKa = 10.46EE361 pKa = 3.97IDD363 pKa = 3.28YY364 pKa = 11.33SRR366 pKa = 11.84FDD368 pKa = 3.65LSISAEE374 pKa = 3.91VLSQFEE380 pKa = 4.47HH381 pKa = 6.26AWVSLVYY388 pKa = 10.07PPSDD392 pKa = 3.62HH393 pKa = 7.12PMFWQTLVATLVTSGFSEE411 pKa = 4.21YY412 pKa = 10.72GITYY416 pKa = 7.39TLPGSRR422 pKa = 11.84CSGDD426 pKa = 3.05PHH428 pKa = 6.12TSVGNGMLNAFLTWLVTYY446 pKa = 10.72DD447 pKa = 3.94KK448 pKa = 11.27DD449 pKa = 3.93CAFYY453 pKa = 11.29CEE455 pKa = 4.79GDD457 pKa = 3.78DD458 pKa = 5.79GIIGCADD465 pKa = 4.45AIGGEE470 pKa = 4.25IEE472 pKa = 4.88IIPDD476 pKa = 3.8LGFMLKK482 pKa = 9.17IDD484 pKa = 4.47RR485 pKa = 11.84YY486 pKa = 10.89DD487 pKa = 4.6HH488 pKa = 7.32IDD490 pKa = 3.64DD491 pKa = 4.59CSFCGMYY498 pKa = 10.98LLDD501 pKa = 5.02DD502 pKa = 4.54GGRR505 pKa = 11.84LHH507 pKa = 6.85MYY509 pKa = 9.92SDD511 pKa = 5.55PIRR514 pKa = 11.84TLSKK518 pKa = 10.41IHH520 pKa = 5.8VCCADD525 pKa = 3.62GKK527 pKa = 9.63PNNLIVAKK535 pKa = 10.37ALSLLNLNPSTPIVTAFCRR554 pKa = 11.84HH555 pKa = 5.44ILRR558 pKa = 11.84VVSSRR563 pKa = 11.84LMNPRR568 pKa = 11.84NRR570 pKa = 11.84NRR572 pKa = 11.84LTATIRR578 pKa = 11.84RR579 pKa = 11.84VAPWVVYY586 pKa = 9.16FPFSYY591 pKa = 10.62EE592 pKa = 4.08PVHH595 pKa = 5.87SQPTPAMRR603 pKa = 11.84AAFAVRR609 pKa = 11.84TGVSPALQQRR619 pKa = 11.84YY620 pKa = 7.37EE621 pKa = 4.15RR622 pKa = 11.84YY623 pKa = 9.77LLSLKK628 pKa = 9.82YY629 pKa = 10.25VPKK632 pKa = 10.15EE633 pKa = 3.56YY634 pKa = 10.84VYY636 pKa = 11.08LKK638 pKa = 10.49RR639 pKa = 11.84DD640 pKa = 3.13VDD642 pKa = 4.06LDD644 pKa = 4.32GINTGLLGEE653 pKa = 4.76FKK655 pKa = 10.8SVLYY659 pKa = 10.74AA660 pKa = 4.07

Molecular weight: 74.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1655

150

845

551.7

61.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.855 ± 0.745

2.477 ± 0.114

5.559 ± 0.6

3.746 ± 0.311

3.988 ± 0.556

5.015 ± 0.477

2.84 ± 0.398

4.411 ± 0.356

2.296 ± 0.713

10.514 ± 1.964

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.752 ± 0.236

2.719 ± 0.28

6.284 ± 0.759

2.236 ± 0.381

8.036 ± 0.562

10.091 ± 0.575

6.284 ± 0.385

7.613 ± 0.812

1.329 ± 0.136

4.955 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski