Streptococcus phage Javan585

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AL70|A0A4D6AL70_9CAUD Uncharacterized protein OS=Streptococcus phage Javan585 OX=2548275 GN=Javan585_0048 PE=4 SV=1
MM1 pKa = 7.83VLEE4 pKa = 4.22VHH6 pKa = 6.95DD7 pKa = 4.64FEE9 pKa = 6.45YY10 pKa = 11.2DD11 pKa = 3.27NQVYY15 pKa = 10.51VDD17 pKa = 4.28FEE19 pKa = 4.3GVRR22 pKa = 11.84YY23 pKa = 9.13EE24 pKa = 4.4VIKK27 pKa = 10.07TFEE30 pKa = 4.21NGDD33 pKa = 4.0IIEE36 pKa = 4.36LTCEE40 pKa = 4.01VVKK43 pKa = 11.15NGTGSNN49 pKa = 3.57

Molecular weight: 5.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AM01|A0A4D6AM01_9CAUD Uncharacterized protein OS=Streptococcus phage Javan585 OX=2548275 GN=Javan585_0040 PE=4 SV=1
MM1 pKa = 7.51NYY3 pKa = 9.61VEE5 pKa = 5.47PIRR8 pKa = 11.84STEE11 pKa = 4.73DD12 pKa = 2.75IDD14 pKa = 4.32MMCDD18 pKa = 3.98YY19 pKa = 10.8LRR21 pKa = 11.84EE22 pKa = 4.28WNEE25 pKa = 3.35RR26 pKa = 11.84NYY28 pKa = 10.51IMFLIGINTSLRR40 pKa = 11.84ISDD43 pKa = 3.48IVILKK48 pKa = 9.16VSNIRR53 pKa = 11.84GHH55 pKa = 5.59YY56 pKa = 9.75IIVIEE61 pKa = 4.5KK62 pKa = 8.51KK63 pKa = 7.23TKK65 pKa = 8.21KK66 pKa = 9.77RR67 pKa = 11.84KK68 pKa = 8.45RR69 pKa = 11.84IKK71 pKa = 10.11MNSLLKK77 pKa = 10.47RR78 pKa = 11.84EE79 pKa = 3.89IEE81 pKa = 4.09RR82 pKa = 11.84YY83 pKa = 9.44IKK85 pKa = 10.2GKK87 pKa = 10.54KK88 pKa = 8.66VGDD91 pKa = 3.55YY92 pKa = 10.68LFQSRR97 pKa = 11.84KK98 pKa = 9.39GKK100 pKa = 9.54NRR102 pKa = 11.84PITRR106 pKa = 11.84QAAYY110 pKa = 10.17CIIKK114 pKa = 10.01IAALDD119 pKa = 3.78CGIEE123 pKa = 4.14NVGTHH128 pKa = 5.32TMRR131 pKa = 11.84KK132 pKa = 6.66TFGYY136 pKa = 10.04HH137 pKa = 5.16YY138 pKa = 9.73YY139 pKa = 10.42KK140 pKa = 10.24KK141 pKa = 10.12HH142 pKa = 6.55KK143 pKa = 9.99DD144 pKa = 2.87IAMLMDD150 pKa = 4.87LFNHH154 pKa = 6.49SSPAITKK161 pKa = 9.84RR162 pKa = 11.84YY163 pKa = 9.66IGINQDD169 pKa = 3.15QQDD172 pKa = 3.34RR173 pKa = 11.84AFASFKK179 pKa = 10.55LGKK182 pKa = 10.1

Molecular weight: 21.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

9460

38

935

185.5

21.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.178 ± 0.462

0.56 ± 0.103

5.899 ± 0.302

7.865 ± 0.436

4.355 ± 0.325

5.994 ± 0.451

1.596 ± 0.162

7.209 ± 0.342

7.928 ± 0.374

8.605 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.18

5.677 ± 0.181

2.939 ± 0.167

4.397 ± 0.279

4.281 ± 0.344

5.899 ± 0.293

5.687 ± 0.391

6.29 ± 0.307

1.216 ± 0.134

3.605 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski