Flavobacteriaceae bacterium M625

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5099 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A504J552|A0A504J552_9FLAO Efflux RND transporter periplasmic adaptor subunit OS=Flavobacteriaceae bacterium M625 OX=2589995 GN=FHK87_12170 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.39KK3 pKa = 8.27ITRR6 pKa = 11.84VLSGFKK12 pKa = 10.05MPVLLFLFSFFSTFGQTGITSLSASYY38 pKa = 11.14KK39 pKa = 10.53DD40 pKa = 4.42INDD43 pKa = 3.58SSNDD47 pKa = 3.23FSNVGNGTNPGYY59 pKa = 10.94SSATTYY65 pKa = 11.61NLLFSTNTTIEE76 pKa = 3.73NDD78 pKa = 3.46YY79 pKa = 10.28TIDD82 pKa = 3.54GSFVAGGTTYY92 pKa = 9.93NTVILIDD99 pKa = 3.48QFEE102 pKa = 4.18VRR104 pKa = 11.84RR105 pKa = 11.84NGTIDD110 pKa = 3.45GQSNRR115 pKa = 11.84QILWFEE121 pKa = 4.28RR122 pKa = 11.84EE123 pKa = 4.38STSGTTINLRR133 pKa = 11.84PSYY136 pKa = 9.54VASVEE141 pKa = 3.88EE142 pKa = 4.23SFRR145 pKa = 11.84GFRR148 pKa = 11.84LNIGSDD154 pKa = 3.1NTFVNTGSNQSNNIEE169 pKa = 3.88RR170 pKa = 11.84FDD172 pKa = 4.52YY173 pKa = 10.98INSSPLSTNTTANAGFAIFEE193 pKa = 4.25RR194 pKa = 11.84DD195 pKa = 3.39GNDD198 pKa = 2.97SFKK201 pKa = 10.65IAAITGLDD209 pKa = 3.33AFGEE213 pKa = 4.48PNAFTPLLTVNTAAYY228 pKa = 9.58GPSLKK233 pKa = 10.1QVNYY237 pKa = 10.18TIFQKK242 pKa = 10.4QDD244 pKa = 3.01SEE246 pKa = 5.41SNLKK250 pKa = 9.44PAEE253 pKa = 4.22NGGPQQIRR261 pKa = 11.84GTFVSFQDD269 pKa = 3.82LGVIPNQAVYY279 pKa = 10.27GYY281 pKa = 10.72VILPADD287 pKa = 3.77ATSVDD292 pKa = 4.36FTTNPTNTDD301 pKa = 3.07SSNGGMDD308 pKa = 4.25AFPGGGFFDD317 pKa = 3.9SDD319 pKa = 3.34GGLTVVNMPDD329 pKa = 3.38ADD331 pKa = 3.88NDD333 pKa = 4.35GVSNTLDD340 pKa = 3.37QDD342 pKa = 3.84DD343 pKa = 5.19DD344 pKa = 4.34NDD346 pKa = 4.97GILDD350 pKa = 3.62INEE353 pKa = 4.53GLDD356 pKa = 3.81CGNGDD361 pKa = 3.16WTAGGTYY368 pKa = 10.3NINQNLGATINNTATYY384 pKa = 7.53NTRR387 pKa = 11.84DD388 pKa = 3.21VDD390 pKa = 4.0LTATLSGGATLSFIRR405 pKa = 11.84IQNAVGGNGQGIVQRR420 pKa = 11.84AQNSNFNTSPVDD432 pKa = 3.36YY433 pKa = 8.94TYY435 pKa = 11.15TFNDD439 pKa = 3.66PVVNLAFDD447 pKa = 4.92FGGLDD452 pKa = 3.18NNDD455 pKa = 3.45FVEE458 pKa = 4.51VRR460 pKa = 11.84AFLGANEE467 pKa = 4.99IILGPDD473 pKa = 2.9NFSVYY478 pKa = 9.75VASDD482 pKa = 2.87VSYY485 pKa = 11.3NGTSSFLATNSGAGNNSNNGSFSVEE510 pKa = 3.41IGDD513 pKa = 4.39PIDD516 pKa = 5.26RR517 pKa = 11.84IVLKK521 pKa = 9.58TGKK524 pKa = 8.36STGSGQVTLHH534 pKa = 6.14TSAFRR539 pKa = 11.84YY540 pKa = 8.45CIPRR544 pKa = 11.84DD545 pKa = 3.28TDD547 pKa = 3.32NNGVYY552 pKa = 10.6DD553 pKa = 4.64HH554 pKa = 7.63LDD556 pKa = 3.42TDD558 pKa = 4.35SDD560 pKa = 4.06NDD562 pKa = 3.66GCADD566 pKa = 4.59AIEE569 pKa = 4.78GGYY572 pKa = 8.72TDD574 pKa = 5.15PDD576 pKa = 3.72DD577 pKa = 6.73DD578 pKa = 5.32EE579 pKa = 5.97ILGNSPVSVDD589 pKa = 3.66GIGRR593 pKa = 11.84VTGQGGYY600 pKa = 9.78SGTRR604 pKa = 11.84SGVITFGTLPSITAQSANQTINTGSNTSFTVTATGSDD641 pKa = 3.33LTYY644 pKa = 10.32RR645 pKa = 11.84WQVDD649 pKa = 3.34TGGGFVDD656 pKa = 4.34IDD658 pKa = 3.77PANTTDD664 pKa = 4.01IYY666 pKa = 10.56TGSDD670 pKa = 3.18TASLNLTNVPVSDD683 pKa = 4.54DD684 pKa = 3.54GNLYY688 pKa = 10.21RR689 pKa = 11.84VIVSSTSFMCGNVEE703 pKa = 4.42SSSILLNVNNYY714 pKa = 7.22TVNLTASVDD723 pKa = 3.74TAVEE727 pKa = 4.03GGIDD731 pKa = 3.88GEE733 pKa = 4.69FTATLNAVNTTGSAIVIPVVMSGTATDD760 pKa = 3.82GTDD763 pKa = 3.06YY764 pKa = 11.21TSIVNITIPNGSDD777 pKa = 2.74TGTVAVDD784 pKa = 3.25AATDD788 pKa = 3.77GLIEE792 pKa = 4.36GDD794 pKa = 3.45EE795 pKa = 4.14TAIASLGTLPSGVVAGTSTSDD816 pKa = 2.88TVTISDD822 pKa = 3.42ATEE825 pKa = 3.79YY826 pKa = 10.07TVNLTASVDD835 pKa = 3.76TAVEE839 pKa = 4.29GGTDD843 pKa = 3.94GEE845 pKa = 4.73FTATLNAVNTTGSAIVIPVVMSGTATDD872 pKa = 3.82GTDD875 pKa = 3.06YY876 pKa = 11.21TSIVNITIPNGSDD889 pKa = 2.74TGTVAVDD896 pKa = 3.25AATDD900 pKa = 3.77GLIEE904 pKa = 4.36GDD906 pKa = 3.37EE907 pKa = 4.14TAIARR912 pKa = 11.84WRR914 pKa = 11.84VYY916 pKa = 11.31SDD918 pKa = 3.86FKK920 pKa = 11.05CGEE923 pKa = 4.4HH924 pKa = 6.71YY925 pKa = 10.13RR926 pKa = 11.84QCYY929 pKa = 9.6CDD931 pKa = 3.59PCSDD935 pKa = 3.01EE936 pKa = 4.25WYY938 pKa = 10.61GYY940 pKa = 10.84

Molecular weight: 98.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A504JA88|A0A504JA88_9FLAO Uncharacterized protein OS=Flavobacteriaceae bacterium M625 OX=2589995 GN=FHK87_09495 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.2RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.66LSVSSEE47 pKa = 3.9LRR49 pKa = 11.84HH50 pKa = 5.43KK51 pKa = 10.29HH52 pKa = 5.04

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5099

0

5099

1710689

38

4048

335.5

38.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.677 ± 0.029

0.774 ± 0.011

5.741 ± 0.028

6.702 ± 0.034

5.199 ± 0.026

6.02 ± 0.033

1.821 ± 0.018

8.634 ± 0.032

8.088 ± 0.05

9.012 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.99 ± 0.016

6.277 ± 0.034

3.32 ± 0.024

3.623 ± 0.02

3.476 ± 0.023

6.61 ± 0.03

5.971 ± 0.042

5.786 ± 0.027

1.07 ± 0.013

4.21 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski