Amino acid dipepetide frequency for Flavobacteriaceae bacterium M625

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.411AlaAla: 3.411 ± 0.062	0.503AlaCys: 0.503 ± 0.018	2.876AlaAsp: 2.876 ± 0.045	3.445AlaGlu: 3.445 ± 0.053	2.976AlaPhe: 2.976 ± 0.043	3.467AlaGly: 3.467 ± 0.058	0.989AlaHis: 0.989 ± 0.024	5.343AlaIle: 5.343 ± 0.068	4.423AlaLys: 4.423 ± 0.062	5.617AlaLeu: 5.617 ± 0.074	1.281AlaMet: 1.281 ± 0.028	3.155AlaAsn: 3.155 ± 0.052	1.726AlaPro: 1.726 ± 0.036	2.166AlaGln: 2.166 ± 0.04	1.809AlaArg: 1.809 ± 0.032	3.929AlaSer: 3.929 ± 0.05	3.43AlaThr: 3.43 ± 0.054	3.426AlaVal: 3.426 ± 0.054	0.523AlaTrp: 0.523 ± 0.018	2.272AlaTyr: 2.272 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	0.399CysAla: 0.399 ± 0.014	0.116CysCys: 0.116 ± 0.01	0.43CysAsp: 0.43 ± 0.016	0.459CysGlu: 0.459 ± 0.02	0.418CysPhe: 0.418 ± 0.016	0.56CysGly: 0.56 ± 0.017	0.167CysHis: 0.167 ± 0.011	0.696CysIle: 0.696 ± 0.021	0.553CysLys: 0.553 ± 0.019	0.647CysLeu: 0.647 ± 0.019	0.152CysMet: 0.152 ± 0.009	0.465CysAsn: 0.465 ± 0.018	0.306CysPro: 0.306 ± 0.017	0.221CysGln: 0.221 ± 0.012	0.216CysArg: 0.216 ± 0.011	0.621CysSer: 0.621 ± 0.022	0.47CysThr: 0.47 ± 0.02	0.419CysVal: 0.419 ± 0.018	0.073CysTrp: 0.073 ± 0.006	0.35CysTyr: 0.35 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.316AspAla: 3.316 ± 0.051	0.435AspCys: 0.435 ± 0.016	3.22AspAsp: 3.22 ± 0.056	3.561AspGlu: 3.561 ± 0.05	3.659AspPhe: 3.659 ± 0.059	3.418AspGly: 3.418 ± 0.06	1.215AspHis: 1.215 ± 0.027	5.204AspIle: 5.204 ± 0.062	4.208AspLys: 4.208 ± 0.059	5.013AspLeu: 5.013 ± 0.058	1.03AspMet: 1.03 ± 0.026	3.492AspAsn: 3.492 ± 0.051	1.91AspPro: 1.91 ± 0.049	2.319AspGln: 2.319 ± 0.032	2.018AspArg: 2.018 ± 0.033	3.258AspSer: 3.258 ± 0.055	3.391AspThr: 3.391 ± 0.05	3.292AspVal: 3.292 ± 0.048	0.822AspTrp: 0.822 ± 0.023	2.625AspTyr: 2.625 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	3.999GluAla: 3.999 ± 0.054	0.366GluCys: 0.366 ± 0.017	3.711GluAsp: 3.711 ± 0.048	5.52GluGlu: 5.52 ± 0.079	2.892GluPhe: 2.892 ± 0.04	3.711GluGly: 3.711 ± 0.049	1.217GluHis: 1.217 ± 0.029	5.999GluIle: 5.999 ± 0.069	6.032GluLys: 6.032 ± 0.076	6.152GluLeu: 6.152 ± 0.07	1.442GluMet: 1.442 ± 0.029	4.682GluAsn: 4.682 ± 0.055	1.539GluPro: 1.539 ± 0.032	2.491GluGln: 2.491 ± 0.042	2.465GluArg: 2.465 ± 0.047	3.495GluSer: 3.495 ± 0.043	3.597GluThr: 3.597 ± 0.052	4.179GluVal: 4.179 ± 0.052	0.682GluTrp: 0.682 ± 0.021	2.853GluTyr: 2.853 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	2.691PheAla: 2.691 ± 0.043	0.472PheCys: 0.472 ± 0.018	3.521PheAsp: 3.521 ± 0.047	3.578PheGlu: 3.578 ± 0.048	2.917PhePhe: 2.917 ± 0.05	3.411PheGly: 3.411 ± 0.056	0.779PheHis: 0.779 ± 0.024	4.144PheIle: 4.144 ± 0.057	3.698PheLys: 3.698 ± 0.051	4.717PheLeu: 4.717 ± 0.058	1.042PheMet: 1.042 ± 0.024	3.131PheAsn: 3.131 ± 0.055	1.617PhePro: 1.617 ± 0.036	1.503PheGln: 1.503 ± 0.029	1.724PheArg: 1.724 ± 0.033	3.976PheSer: 3.976 ± 0.055	3.253PheThr: 3.253 ± 0.053	2.905PheVal: 2.905 ± 0.044	0.604PheTrp: 0.604 ± 0.022	2.303PheTyr: 2.303 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	3.667GlyAla: 3.667 ± 0.065	0.569GlyCys: 0.569 ± 0.024	3.296GlyAsp: 3.296 ± 0.058	3.491GlyGlu: 3.491 ± 0.052	3.572GlyPhe: 3.572 ± 0.054	4.096GlyGly: 4.096 ± 0.078	0.995GlyHis: 0.995 ± 0.028	5.447GlyIle: 5.447 ± 0.066	4.736GlyLys: 4.736 ± 0.066	5.126GlyLeu: 5.126 ± 0.067	1.336GlyMet: 1.336 ± 0.03	3.718GlyAsn: 3.718 ± 0.064	1.207GlyPro: 1.207 ± 0.038	1.791GlyGln: 1.791 ± 0.04	1.988GlyArg: 1.988 ± 0.033	3.758GlySer: 3.758 ± 0.057	3.779GlyThr: 3.779 ± 0.07	4.021GlyVal: 4.021 ± 0.06	0.751GlyTrp: 0.751 ± 0.025	2.852GlyTyr: 2.852 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	0.856HisAla: 0.856 ± 0.026	0.189HisCys: 0.189 ± 0.011	0.903HisAsp: 0.903 ± 0.024	0.966HisGlu: 0.966 ± 0.023	1.159HisPhe: 1.159 ± 0.03	0.952HisGly: 0.952 ± 0.025	0.507HisHis: 0.507 ± 0.02	1.56HisIle: 1.56 ± 0.034	1.495HisLys: 1.495 ± 0.027	1.745HisLeu: 1.745 ± 0.033	0.314HisMet: 0.314 ± 0.012	1.055HisAsn: 1.055 ± 0.026	0.905HisPro: 0.905 ± 0.027	0.8HisGln: 0.8 ± 0.026	0.69HisArg: 0.69 ± 0.021	1.067HisSer: 1.067 ± 0.027	1.092HisThr: 1.092 ± 0.025	0.853HisVal: 0.853 ± 0.022	0.231HisTrp: 0.231 ± 0.013	0.871HisTyr: 0.871 ± 0.023	0.001HisXaa: 0.001 ± 0.001
Ile	5.567IleAla: 5.567 ± 0.066	0.676IleCys: 0.676 ± 0.022	5.568IleAsp: 5.568 ± 0.062	6.096IleGlu: 6.096 ± 0.066	3.892IlePhe: 3.892 ± 0.055	5.149IleGly: 5.149 ± 0.069	1.563IleHis: 1.563 ± 0.033	6.972IleIle: 6.972 ± 0.077	6.791IleLys: 6.791 ± 0.08	7.535IleLeu: 7.535 ± 0.082	1.371IleMet: 1.371 ± 0.03	5.168IleAsn: 5.168 ± 0.062	3.488IlePro: 3.488 ± 0.05	3.078IleGln: 3.078 ± 0.045	2.908IleArg: 2.908 ± 0.046	6.323IleSer: 6.323 ± 0.063	5.487IleThr: 5.487 ± 0.074	4.919IleVal: 4.919 ± 0.06	0.79IleTrp: 0.79 ± 0.021	2.996IleTyr: 2.996 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	4.54LysAla: 4.54 ± 0.061	0.372LysCys: 0.372 ± 0.016	4.726LysAsp: 4.726 ± 0.059	6.99LysGlu: 6.99 ± 0.081	2.802LysPhe: 2.802 ± 0.044	4.593LysGly: 4.593 ± 0.066	1.477LysHis: 1.477 ± 0.034	6.879LysIle: 6.879 ± 0.088	7.722LysLys: 7.722 ± 0.087	6.456LysLeu: 6.456 ± 0.076	1.841LysMet: 1.841 ± 0.032	5.813LysAsn: 5.813 ± 0.065	2.346LysPro: 2.346 ± 0.045	2.867LysGln: 2.867 ± 0.045	3.05LysArg: 3.05 ± 0.053	4.803LysSer: 4.803 ± 0.062	4.872LysThr: 4.872 ± 0.054	4.633LysVal: 4.633 ± 0.064	0.825LysTrp: 0.825 ± 0.021	3.268LysTyr: 3.268 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	4.955LeuAla: 4.955 ± 0.062	0.727LeuCys: 0.727 ± 0.021	5.171LeuAsp: 5.171 ± 0.063	6.037LeuGlu: 6.037 ± 0.064	4.872LeuPhe: 4.872 ± 0.068	5.419LeuGly: 5.419 ± 0.059	1.64LeuHis: 1.64 ± 0.032	7.283LeuIle: 7.283 ± 0.085	7.576LeuLys: 7.576 ± 0.087	8.425LeuLeu: 8.425 ± 0.098	1.83LeuMet: 1.83 ± 0.033	5.269LeuAsn: 5.269 ± 0.055	3.368LeuPro: 3.368 ± 0.044	3.57LeuGln: 3.57 ± 0.055	3.058LeuArg: 3.058 ± 0.042	6.884LeuSer: 6.884 ± 0.063	4.919LeuThr: 4.919 ± 0.061	4.834LeuVal: 4.834 ± 0.058	0.826LeuTrp: 0.826 ± 0.026	3.451LeuTyr: 3.451 ± 0.05	0.001LeuXaa: 0.001 ± 0.001
Met	1.247MetAla: 1.247 ± 0.031	0.12MetCys: 0.12 ± 0.008	0.979MetAsp: 0.979 ± 0.024	1.036MetGlu: 1.036 ± 0.027	0.833MetPhe: 0.833 ± 0.035	1.18MetGly: 1.18 ± 0.026	0.378MetHis: 0.378 ± 0.013	1.72MetIle: 1.72 ± 0.037	2.018MetLys: 2.018 ± 0.036	1.792MetLeu: 1.792 ± 0.038	0.525MetMet: 0.525 ± 0.017	1.311MetAsn: 1.311 ± 0.029	0.758MetPro: 0.758 ± 0.021	0.79MetGln: 0.79 ± 0.023	0.79MetArg: 0.79 ± 0.022	1.322MetSer: 1.322 ± 0.029	1.119MetThr: 1.119 ± 0.028	1.125MetVal: 1.125 ± 0.025	0.139MetTrp: 0.139 ± 0.009	0.723MetTyr: 0.723 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.652AsnAla: 3.652 ± 0.055	0.479AsnCys: 0.479 ± 0.018	3.6AsnAsp: 3.6 ± 0.053	3.81AsnGlu: 3.81 ± 0.054	3.013AsnPhe: 3.013 ± 0.048	3.939AsnGly: 3.939 ± 0.058	1.147AsnHis: 1.147 ± 0.026	5.389AsnIle: 5.389 ± 0.064	4.443AsnLys: 4.443 ± 0.054	5.305AsnLeu: 5.305 ± 0.061	1.211AsnMet: 1.211 ± 0.029	4.054AsnAsn: 4.054 ± 0.067	2.687AsnPro: 2.687 ± 0.046	2.501AsnGln: 2.501 ± 0.045	2.334AsnArg: 2.334 ± 0.04	3.918AsnSer: 3.918 ± 0.051	4.407AsnThr: 4.407 ± 0.062	3.362AsnVal: 3.362 ± 0.06	0.796AsnTrp: 0.796 ± 0.028	2.722AsnTyr: 2.722 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	1.599ProAla: 1.599 ± 0.035	0.216ProCys: 0.216 ± 0.013	2.237ProAsp: 2.237 ± 0.04	2.745ProGlu: 2.745 ± 0.043	1.812ProPhe: 1.812 ± 0.036	1.812ProGly: 1.812 ± 0.043	0.574ProHis: 0.574 ± 0.02	2.856ProIle: 2.856 ± 0.05	2.789ProLys: 2.789 ± 0.043	2.843ProLeu: 2.843 ± 0.043	0.644ProMet: 0.644 ± 0.021	2.323ProAsn: 2.323 ± 0.039	0.853ProPro: 0.853 ± 0.028	1.122ProGln: 1.122 ± 0.026	0.932ProArg: 0.932 ± 0.026	2.115ProSer: 2.115 ± 0.036	1.83ProThr: 1.83 ± 0.048	2.192ProVal: 2.192 ± 0.041	0.334ProTrp: 0.334 ± 0.016	1.374ProTyr: 1.374 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	1.833GlnAla: 1.833 ± 0.037	0.221GlnCys: 0.221 ± 0.013	1.805GlnAsp: 1.805 ± 0.034	2.743GlnGlu: 2.743 ± 0.046	1.707GlnPhe: 1.707 ± 0.036	1.836GlnGly: 1.836 ± 0.035	0.685GlnHis: 0.685 ± 0.021	2.965GlnIle: 2.965 ± 0.045	3.625GlnLys: 3.625 ± 0.053	3.486GlnLeu: 3.486 ± 0.045	0.74GlnMet: 0.74 ± 0.02	2.548GlnAsn: 2.548 ± 0.039	1.108GlnPro: 1.108 ± 0.029	1.664GlnGln: 1.664 ± 0.037	1.309GlnArg: 1.309 ± 0.031	2.162GlnSer: 2.162 ± 0.036	1.872GlnThr: 1.872 ± 0.037	1.888GlnVal: 1.888 ± 0.031	0.42GlnTrp: 0.42 ± 0.018	1.615GlnTyr: 1.615 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	1.92ArgAla: 1.92 ± 0.037	0.231ArgCys: 0.231 ± 0.012	1.841ArgAsp: 1.841 ± 0.036	2.217ArgGlu: 2.217 ± 0.041	2.003ArgPhe: 2.003 ± 0.039	1.939ArgGly: 1.939 ± 0.037	0.567ArgHis: 0.567 ± 0.017	3.169ArgIle: 3.169 ± 0.042	2.881ArgLys: 2.881 ± 0.049	3.357ArgLeu: 3.357 ± 0.052	0.851ArgMet: 0.851 ± 0.022	2.221ArgAsn: 2.221 ± 0.041	1.078ArgPro: 1.078 ± 0.028	1.093ArgGln: 1.093 ± 0.028	1.388ArgArg: 1.388 ± 0.035	2.033ArgSer: 2.033 ± 0.043	1.869ArgThr: 1.869 ± 0.032	2.059ArgVal: 2.059 ± 0.031	0.397ArgTrp: 0.397 ± 0.015	1.645ArgTyr: 1.645 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	3.29SerAla: 3.29 ± 0.047	0.724SerCys: 0.724 ± 0.023	3.711SerAsp: 3.711 ± 0.051	4.117SerGlu: 4.117 ± 0.049	3.982SerPhe: 3.982 ± 0.045	4.553SerGly: 4.553 ± 0.063	1.084SerHis: 1.084 ± 0.025	5.981SerIle: 5.981 ± 0.06	5.368SerLys: 5.368 ± 0.063	5.958SerLeu: 5.958 ± 0.056	1.201SerMet: 1.201 ± 0.027	4.123SerAsn: 4.123 ± 0.058	2.063SerPro: 2.063 ± 0.035	2.156SerGln: 2.156 ± 0.039	2.214SerArg: 2.214 ± 0.041	4.586SerSer: 4.586 ± 0.069	3.687SerThr: 3.687 ± 0.05	3.71SerVal: 3.71 ± 0.048	0.72SerTrp: 0.72 ± 0.024	2.873SerTyr: 2.873 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	3.389ThrAla: 3.389 ± 0.05	0.374ThrCys: 0.374 ± 0.017	3.271ThrAsp: 3.271 ± 0.057	3.457ThrGlu: 3.457 ± 0.051	3.159ThrPhe: 3.159 ± 0.048	3.783ThrGly: 3.783 ± 0.07	1.071ThrHis: 1.071 ± 0.027	5.838ThrIle: 5.838 ± 0.078	4.232ThrLys: 4.232 ± 0.047	5.521ThrLeu: 5.521 ± 0.055	0.932ThrMet: 0.932 ± 0.025	3.531ThrAsn: 3.531 ± 0.064	2.571ThrPro: 2.571 ± 0.062	2.075ThrGln: 2.075 ± 0.038	1.785ThrArg: 1.785 ± 0.036	4.205ThrSer: 4.205 ± 0.061	3.82ThrThr: 3.82 ± 0.066	3.641ThrVal: 3.641 ± 0.068	0.569ThrTrp: 0.569 ± 0.023	2.489ThrTyr: 2.489 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	3.496ValAla: 3.496 ± 0.051	0.482ValCys: 0.482 ± 0.015	3.372ValAsp: 3.372 ± 0.048	3.684ValGlu: 3.684 ± 0.046	3.258ValPhe: 3.258 ± 0.049	3.296ValGly: 3.296 ± 0.051	0.949ValHis: 0.949 ± 0.022	4.903ValIle: 4.903 ± 0.058	4.082ValLys: 4.082 ± 0.057	5.622ValLeu: 5.622 ± 0.069	1.177ValMet: 1.177 ± 0.025	3.369ValAsn: 3.369 ± 0.05	1.982ValPro: 1.982 ± 0.036	1.809ValGln: 1.809 ± 0.032	1.885ValArg: 1.885 ± 0.039	4.33ValSer: 4.33 ± 0.056	3.618ValThr: 3.618 ± 0.08	3.665ValVal: 3.665 ± 0.054	0.558ValTrp: 0.558 ± 0.018	2.328ValTyr: 2.328 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.574TrpAla: 0.574 ± 0.021	0.105TrpCys: 0.105 ± 0.009	0.656TrpAsp: 0.656 ± 0.023	0.649TrpGlu: 0.649 ± 0.023	0.565TrpPhe: 0.565 ± 0.021	0.624TrpGly: 0.624 ± 0.017	0.221TrpHis: 0.221 ± 0.011	0.861TrpIle: 0.861 ± 0.024	0.875TrpLys: 0.875 ± 0.026	0.975TrpLeu: 0.975 ± 0.023	0.295TrpMet: 0.295 ± 0.011	0.714TrpAsn: 0.714 ± 0.029	0.239TrpPro: 0.239 ± 0.013	0.432TrpGln: 0.432 ± 0.016	0.435TrpArg: 0.435 ± 0.017	0.675TrpSer: 0.675 ± 0.025	0.538TrpThr: 0.538 ± 0.023	0.63TrpVal: 0.63 ± 0.019	0.161TrpTrp: 0.161 ± 0.01	0.477TrpTyr: 0.477 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.365TyrAla: 2.365 ± 0.035	0.363TyrCys: 0.363 ± 0.014	2.511TyrAsp: 2.511 ± 0.047	2.419TyrGlu: 2.419 ± 0.041	2.497TyrPhe: 2.497 ± 0.041	2.456TyrGly: 2.456 ± 0.047	0.986TyrHis: 0.986 ± 0.025	3.128TyrIle: 3.128 ± 0.046	3.328TyrLys: 3.328 ± 0.048	3.824TyrLeu: 3.824 ± 0.048	0.688TyrMet: 0.688 ± 0.019	2.625TyrAsn: 2.625 ± 0.046	1.452TyrPro: 1.452 ± 0.03	1.785TyrGln: 1.785 ± 0.037	1.759TyrArg: 1.759 ± 0.034	2.642TyrSer: 2.642 ± 0.045	2.662TyrThr: 2.662 ± 0.047	2.113TyrVal: 2.113 ± 0.035	0.48TyrTrp: 0.48 ± 0.017	2.011TyrTyr: 2.011 ± 0.04	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5099 proteins (1710689 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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