Microbacterium phage Gina

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Metamorphoovirus; unclassified Metamorphoovirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V966|A0A649V966_9CAUD DUF2786 domain-containing protein OS=Microbacterium phage Gina OX=2656548 GN=5 PE=4 SV=1
MM1 pKa = 7.99DD2 pKa = 4.43EE3 pKa = 4.85ALASLLDD10 pKa = 4.38GYY12 pKa = 8.25TLYY15 pKa = 11.17ASDD18 pKa = 6.15DD19 pKa = 3.53IAKK22 pKa = 10.28SDD24 pKa = 4.09LEE26 pKa = 4.3LVHH29 pKa = 7.02DD30 pKa = 3.62ICEE33 pKa = 4.33TVLCDD38 pKa = 5.54AEE40 pKa = 4.33ATDD43 pKa = 3.98TLSTLVSVAMNHH55 pKa = 5.51EE56 pKa = 4.53RR57 pKa = 11.84EE58 pKa = 4.45CAGG61 pKa = 3.56

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V9B1|A0A649V9B1_9CAUD UDP-glucose dehydrogenase OS=Microbacterium phage Gina OX=2656548 GN=90 PE=4 SV=1
MM1 pKa = 7.77TITTPTVPEE10 pKa = 3.97PVQEE14 pKa = 4.02VGRR17 pKa = 11.84AFEE20 pKa = 4.1KK21 pKa = 10.46RR22 pKa = 11.84RR23 pKa = 11.84EE24 pKa = 3.9IAQRR28 pKa = 11.84LADD31 pKa = 3.53QDD33 pKa = 3.98RR34 pKa = 11.84YY35 pKa = 10.52IGAAVRR41 pKa = 11.84TARR44 pKa = 11.84TAGHH48 pKa = 6.15SWAEE52 pKa = 3.84MARR55 pKa = 11.84AAKK58 pKa = 10.32VSDD61 pKa = 3.6VAILKK66 pKa = 9.57AARR69 pKa = 11.84RR70 pKa = 11.84PEE72 pKa = 3.85KK73 pKa = 10.61AAA75 pKa = 3.79

Molecular weight: 8.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

17034

32

955

177.4

19.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.988 ± 0.457

0.963 ± 0.122

6.687 ± 0.353

6.293 ± 0.348

2.483 ± 0.114

8.835 ± 0.352

1.949 ± 0.184

4.327 ± 0.276

2.472 ± 0.204

7.779 ± 0.388

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.419 ± 0.152

2.601 ± 0.176

5.918 ± 0.247

2.988 ± 0.174

7.01 ± 0.399

6.012 ± 0.26

7.168 ± 0.374

7.55 ± 0.217

2.09 ± 0.175

2.472 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski