Amino acid dipepetide frequency for Microbacterium phage Gina

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.378AlaAla: 17.378 ± 1.861
0.822AlaCys: 0.822 ± 0.243
7.632AlaAsp: 7.632 ± 0.77
7.574AlaGlu: 7.574 ± 0.881
3.757AlaPhe: 3.757 ± 0.518
9.452AlaGly: 9.452 ± 0.863
2.055AlaHis: 2.055 ± 0.407
4.579AlaIle: 4.579 ± 0.52
3.405AlaLys: 3.405 ± 0.478
11.683AlaLeu: 11.683 ± 1.388
2.818AlaMet: 2.818 ± 0.336
2.407AlaAsn: 2.407 ± 0.477
6.282AlaPro: 6.282 ± 0.604
3.934AlaGln: 3.934 ± 0.515
7.75AlaArg: 7.75 ± 0.832
7.28AlaSer: 7.28 ± 0.718
8.924AlaThr: 8.924 ± 0.899
7.221AlaVal: 7.221 ± 0.737
2.759AlaTrp: 2.759 ± 0.41
2.172AlaTyr: 2.172 ± 0.381
0.0AlaXaa: 0.0 ± 0.0
Cys
0.939CysAla: 0.939 ± 0.217
0.235CysCys: 0.235 ± 0.147
1.174CysAsp: 1.174 ± 0.285
0.646CysGlu: 0.646 ± 0.199
0.176CysPhe: 0.176 ± 0.101
1.409CysGly: 1.409 ± 0.267
0.411CysHis: 0.411 ± 0.151
0.294CysIle: 0.294 ± 0.117
0.0CysLys: 0.0 ± 0.0
0.705CysLeu: 0.705 ± 0.176
0.117CysMet: 0.117 ± 0.101
0.235CysAsn: 0.235 ± 0.111
0.822CysPro: 0.822 ± 0.218
0.117CysGln: 0.117 ± 0.072
0.822CysArg: 0.822 ± 0.261
0.235CysSer: 0.235 ± 0.116
0.294CysThr: 0.294 ± 0.115
0.822CysVal: 0.822 ± 0.216
0.059CysTrp: 0.059 ± 0.049
0.117CysTyr: 0.117 ± 0.076
0.0CysXaa: 0.0 ± 0.0
Asp
8.454AspAla: 8.454 ± 0.808
0.822AspCys: 0.822 ± 0.233
4.873AspAsp: 4.873 ± 0.577
5.343AspGlu: 5.343 ± 0.706
1.703AspPhe: 1.703 ± 0.333
6.341AspGly: 6.341 ± 0.617
1.174AspHis: 1.174 ± 0.262
2.701AspIle: 2.701 ± 0.383
1.585AspLys: 1.585 ± 0.284
5.695AspLeu: 5.695 ± 0.529
1.996AspMet: 1.996 ± 0.341
1.703AspAsn: 1.703 ± 0.327
3.699AspPro: 3.699 ± 0.568
1.996AspGln: 1.996 ± 0.338
4.932AspArg: 4.932 ± 0.63
2.818AspSer: 2.818 ± 0.387
3.64AspThr: 3.64 ± 0.476
4.697AspVal: 4.697 ± 0.563
1.526AspTrp: 1.526 ± 0.303
1.174AspTyr: 1.174 ± 0.342
0.0AspXaa: 0.0 ± 0.0
Glu
7.75GluAla: 7.75 ± 0.822
0.528GluCys: 0.528 ± 0.179
4.051GluAsp: 4.051 ± 0.581
4.697GluGlu: 4.697 ± 0.659
1.761GluPhe: 1.761 ± 0.277
4.403GluGly: 4.403 ± 0.595
1.937GluHis: 1.937 ± 0.383
1.292GluIle: 1.292 ± 0.301
1.937GluLys: 1.937 ± 0.299
3.346GluLeu: 3.346 ± 0.426
1.233GluMet: 1.233 ± 0.247
1.644GluAsn: 1.644 ± 0.29
4.403GluPro: 4.403 ± 0.524
2.172GluGln: 2.172 ± 0.362
5.343GluArg: 5.343 ± 0.607
4.051GluSer: 4.051 ± 0.589
3.757GluThr: 3.757 ± 0.557
5.695GluVal: 5.695 ± 0.689
1.35GluTrp: 1.35 ± 0.276
1.585GluTyr: 1.585 ± 0.397
0.0GluXaa: 0.0 ± 0.0
Phe
2.231PheAla: 2.231 ± 0.429
0.352PheCys: 0.352 ± 0.139
2.348PheAsp: 2.348 ± 0.331
2.114PheGlu: 2.114 ± 0.354
0.646PhePhe: 0.646 ± 0.226
2.114PheGly: 2.114 ± 0.365
0.294PheHis: 0.294 ± 0.129
1.174PheIle: 1.174 ± 0.306
0.587PheLys: 0.587 ± 0.225
1.585PheLeu: 1.585 ± 0.309
0.587PheMet: 0.587 ± 0.162
0.587PheAsn: 0.587 ± 0.197
1.057PhePro: 1.057 ± 0.257
0.646PheGln: 0.646 ± 0.205
1.526PheArg: 1.526 ± 0.268
1.233PheSer: 1.233 ± 0.267
2.348PheThr: 2.348 ± 0.37
2.231PheVal: 2.231 ± 0.372
0.47PheTrp: 0.47 ± 0.181
0.705PheTyr: 0.705 ± 0.196
0.0PheXaa: 0.0 ± 0.0
Gly
7.808GlyAla: 7.808 ± 0.891
0.881GlyCys: 0.881 ± 0.246
6.223GlyAsp: 6.223 ± 0.599
4.579GlyGlu: 4.579 ± 0.485
2.407GlyPhe: 2.407 ± 0.298
9.1GlyGly: 9.1 ± 0.861
2.348GlyHis: 2.348 ± 0.393
3.934GlyIle: 3.934 ± 0.554
2.466GlyLys: 2.466 ± 0.445
7.339GlyLeu: 7.339 ± 0.68
2.994GlyMet: 2.994 ± 0.464
2.407GlyAsn: 2.407 ± 0.448
3.875GlyPro: 3.875 ± 0.41
2.818GlyGln: 2.818 ± 0.48
5.577GlyArg: 5.577 ± 0.608
5.871GlySer: 5.871 ± 0.755
6.517GlyThr: 6.517 ± 0.88
6.106GlyVal: 6.106 ± 0.583
2.583GlyTrp: 2.583 ± 0.376
2.466GlyTyr: 2.466 ± 0.397
0.0GlyXaa: 0.0 ± 0.0
His
1.761HisAla: 1.761 ± 0.377
0.176HisCys: 0.176 ± 0.1
1.174HisAsp: 1.174 ± 0.252
1.468HisGlu: 1.468 ± 0.313
0.646HisPhe: 0.646 ± 0.163
1.82HisGly: 1.82 ± 0.376
0.528HisHis: 0.528 ± 0.229
0.998HisIle: 0.998 ± 0.241
0.352HisLys: 0.352 ± 0.165
1.761HisLeu: 1.761 ± 0.317
0.411HisMet: 0.411 ± 0.133
0.294HisAsn: 0.294 ± 0.126
1.82HisPro: 1.82 ± 0.366
0.235HisGln: 0.235 ± 0.124
1.409HisArg: 1.409 ± 0.288
1.468HisSer: 1.468 ± 0.252
1.057HisThr: 1.057 ± 0.258
0.998HisVal: 0.998 ± 0.26
0.528HisTrp: 0.528 ± 0.19
0.587HisTyr: 0.587 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
4.873IleAla: 4.873 ± 0.544
0.235IleCys: 0.235 ± 0.137
3.523IleAsp: 3.523 ± 0.449
3.816IleGlu: 3.816 ± 0.424
0.294IlePhe: 0.294 ± 0.121
3.757IleGly: 3.757 ± 0.405
0.998IleHis: 0.998 ± 0.206
2.114IleIle: 2.114 ± 0.437
0.822IleLys: 0.822 ± 0.199
2.525IleLeu: 2.525 ± 0.295
0.646IleMet: 0.646 ± 0.182
0.998IleAsn: 0.998 ± 0.307
2.466IlePro: 2.466 ± 0.625
1.409IleGln: 1.409 ± 0.285
2.525IleArg: 2.525 ± 0.4
2.348IleSer: 2.348 ± 0.445
3.992IleThr: 3.992 ± 0.5
4.168IleVal: 4.168 ± 0.424
0.763IleTrp: 0.763 ± 0.196
0.998IleTyr: 0.998 ± 0.253
0.0IleXaa: 0.0 ± 0.0
Lys
3.64LysAla: 3.64 ± 0.556
0.587LysCys: 0.587 ± 0.173
1.292LysAsp: 1.292 ± 0.368
0.646LysGlu: 0.646 ± 0.201
0.587LysPhe: 0.587 ± 0.198
2.407LysGly: 2.407 ± 0.367
0.587LysHis: 0.587 ± 0.201
0.881LysIle: 0.881 ± 0.263
0.411LysLys: 0.411 ± 0.179
0.939LysLeu: 0.939 ± 0.286
0.352LysMet: 0.352 ± 0.126
0.646LysAsn: 0.646 ± 0.166
2.055LysPro: 2.055 ± 0.377
0.705LysGln: 0.705 ± 0.194
2.466LysArg: 2.466 ± 0.42
1.703LysSer: 1.703 ± 0.341
0.881LysThr: 0.881 ± 0.263
2.818LysVal: 2.818 ± 0.44
0.294LysTrp: 0.294 ± 0.138
0.822LysTyr: 0.822 ± 0.217
0.0LysXaa: 0.0 ± 0.0
Leu
9.981LeuAla: 9.981 ± 0.768
0.705LeuCys: 0.705 ± 0.193
5.343LeuAsp: 5.343 ± 0.614
4.11LeuGlu: 4.11 ± 0.496
1.468LeuPhe: 1.468 ± 0.351
7.28LeuGly: 7.28 ± 0.989
0.939LeuHis: 0.939 ± 0.224
4.403LeuIle: 4.403 ± 0.639
0.646LeuLys: 0.646 ± 0.177
6.282LeuLeu: 6.282 ± 0.889
1.526LeuMet: 1.526 ± 0.26
1.703LeuAsn: 1.703 ± 0.343
4.99LeuPro: 4.99 ± 0.444
1.526LeuGln: 1.526 ± 0.253
5.049LeuArg: 5.049 ± 0.625
5.519LeuSer: 5.519 ± 0.74
7.045LeuThr: 7.045 ± 0.746
6.165LeuVal: 6.165 ± 0.62
1.057LeuTrp: 1.057 ± 0.22
2.055LeuTyr: 2.055 ± 0.382
0.0LeuXaa: 0.0 ± 0.0
Met
2.642MetAla: 2.642 ± 0.397
0.176MetCys: 0.176 ± 0.099
1.526MetAsp: 1.526 ± 0.293
1.057MetGlu: 1.057 ± 0.245
0.47MetPhe: 0.47 ± 0.193
1.937MetGly: 1.937 ± 0.389
0.352MetHis: 0.352 ± 0.147
1.585MetIle: 1.585 ± 0.324
0.47MetLys: 0.47 ± 0.176
1.761MetLeu: 1.761 ± 0.31
0.646MetMet: 0.646 ± 0.213
0.881MetAsn: 0.881 ± 0.237
1.233MetPro: 1.233 ± 0.284
0.646MetGln: 0.646 ± 0.197
1.703MetArg: 1.703 ± 0.294
2.583MetSer: 2.583 ± 0.366
2.348MetThr: 2.348 ± 0.313
1.174MetVal: 1.174 ± 0.266
0.352MetTrp: 0.352 ± 0.13
0.646MetTyr: 0.646 ± 0.215
0.0MetXaa: 0.0 ± 0.0
Asn
3.934AsnAla: 3.934 ± 0.463
0.294AsnCys: 0.294 ± 0.124
1.468AsnAsp: 1.468 ± 0.258
0.939AsnGlu: 0.939 ± 0.238
0.352AsnPhe: 0.352 ± 0.144
3.17AsnGly: 3.17 ± 0.417
0.411AsnHis: 0.411 ± 0.178
1.057AsnIle: 1.057 ± 0.304
0.176AsnLys: 0.176 ± 0.086
2.642AsnLeu: 2.642 ± 0.338
0.47AsnMet: 0.47 ± 0.17
0.47AsnAsn: 0.47 ± 0.17
1.526AsnPro: 1.526 ± 0.378
0.646AsnGln: 0.646 ± 0.216
2.055AsnArg: 2.055 ± 0.316
1.585AsnSer: 1.585 ± 0.317
1.82AsnThr: 1.82 ± 0.288
1.761AsnVal: 1.761 ± 0.32
0.47AsnTrp: 0.47 ± 0.138
0.763AsnTyr: 0.763 ± 0.225
0.0AsnXaa: 0.0 ± 0.0
Pro
5.93ProAla: 5.93 ± 0.652
0.528ProCys: 0.528 ± 0.209
4.11ProAsp: 4.11 ± 0.694
4.873ProGlu: 4.873 ± 0.554
1.585ProPhe: 1.585 ± 0.287
5.166ProGly: 5.166 ± 0.571
1.35ProHis: 1.35 ± 0.256
2.114ProIle: 2.114 ± 0.301
1.996ProLys: 1.996 ± 0.396
3.992ProLeu: 3.992 ± 0.43
1.35ProMet: 1.35 ± 0.266
1.996ProAsn: 1.996 ± 0.422
3.757ProPro: 3.757 ± 0.577
2.055ProGln: 2.055 ± 0.532
3.288ProArg: 3.288 ± 0.468
3.699ProSer: 3.699 ± 0.522
3.581ProThr: 3.581 ± 0.38
5.284ProVal: 5.284 ± 0.588
0.939ProTrp: 0.939 ± 0.223
1.585ProTyr: 1.585 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
4.286GlnAla: 4.286 ± 0.731
0.117GlnCys: 0.117 ± 0.086
1.292GlnAsp: 1.292 ± 0.3
1.35GlnGlu: 1.35 ± 0.302
1.174GlnPhe: 1.174 ± 0.259
2.172GlnGly: 2.172 ± 0.392
0.998GlnHis: 0.998 ± 0.24
1.761GlnIle: 1.761 ± 0.377
0.763GlnLys: 0.763 ± 0.239
1.233GlnLeu: 1.233 ± 0.38
0.998GlnMet: 0.998 ± 0.242
1.115GlnAsn: 1.115 ± 0.302
1.937GlnPro: 1.937 ± 0.318
0.998GlnGln: 0.998 ± 0.214
2.407GlnArg: 2.407 ± 0.484
1.585GlnSer: 1.585 ± 0.327
1.996GlnThr: 1.996 ± 0.301
2.114GlnVal: 2.114 ± 0.303
0.822GlnTrp: 0.822 ± 0.226
0.763GlnTyr: 0.763 ± 0.195
0.0GlnXaa: 0.0 ± 0.0
Arg
7.985ArgAla: 7.985 ± 0.677
0.763ArgCys: 0.763 ± 0.188
4.579ArgAsp: 4.579 ± 0.627
4.697ArgGlu: 4.697 ± 0.614
1.937ArgPhe: 1.937 ± 0.39
5.225ArgGly: 5.225 ± 0.651
1.174ArgHis: 1.174 ± 0.288
2.877ArgIle: 2.877 ± 0.44
2.114ArgLys: 2.114 ± 0.371
5.93ArgLeu: 5.93 ± 0.556
1.996ArgMet: 1.996 ± 0.417
1.879ArgAsn: 1.879 ± 0.376
3.523ArgPro: 3.523 ± 0.524
2.172ArgGln: 2.172 ± 0.356
6.693ArgArg: 6.693 ± 1.005
3.464ArgSer: 3.464 ± 0.575
3.992ArgThr: 3.992 ± 0.503
4.932ArgVal: 4.932 ± 0.605
1.82ArgTrp: 1.82 ± 0.327
2.348ArgTyr: 2.348 ± 0.428
0.0ArgXaa: 0.0 ± 0.0
Ser
6.869SerAla: 6.869 ± 0.866
0.294SerCys: 0.294 ± 0.125
4.462SerAsp: 4.462 ± 0.594
3.992SerGlu: 3.992 ± 0.506
1.409SerPhe: 1.409 ± 0.328
5.225SerGly: 5.225 ± 0.668
0.939SerHis: 0.939 ± 0.241
3.17SerIle: 3.17 ± 0.5
1.526SerLys: 1.526 ± 0.337
4.873SerLeu: 4.873 ± 0.48
1.82SerMet: 1.82 ± 0.313
2.055SerAsn: 2.055 ± 0.363
3.464SerPro: 3.464 ± 0.388
1.468SerGln: 1.468 ± 0.267
3.581SerArg: 3.581 ± 0.46
2.935SerSer: 2.935 ± 0.507
5.108SerThr: 5.108 ± 0.624
4.403SerVal: 4.403 ± 0.515
0.822SerTrp: 0.822 ± 0.256
1.703SerTyr: 1.703 ± 0.358
0.0SerXaa: 0.0 ± 0.0
Thr
9.687ThrAla: 9.687 ± 1.152
0.587ThrCys: 0.587 ± 0.178
4.521ThrAsp: 4.521 ± 0.544
3.523ThrGlu: 3.523 ± 0.521
1.703ThrPhe: 1.703 ± 0.301
7.515ThrGly: 7.515 ± 1.058
0.998ThrHis: 0.998 ± 0.234
3.17ThrIle: 3.17 ± 0.39
1.937ThrLys: 1.937 ± 0.366
6.106ThrLeu: 6.106 ± 0.692
1.115ThrMet: 1.115 ± 0.236
1.761ThrAsn: 1.761 ± 0.314
5.284ThrPro: 5.284 ± 0.644
2.231ThrGln: 2.231 ± 0.28
4.168ThrArg: 4.168 ± 0.527
3.699ThrSer: 3.699 ± 0.497
4.99ThrThr: 4.99 ± 0.772
5.871ThrVal: 5.871 ± 0.559
1.174ThrTrp: 1.174 ± 0.251
1.644ThrTyr: 1.644 ± 0.258
0.0ThrXaa: 0.0 ± 0.0
Val
9.1ValAla: 9.1 ± 0.625
0.705ValCys: 0.705 ± 0.19
3.64ValAsp: 3.64 ± 0.56
4.697ValGlu: 4.697 ± 0.545
1.585ValPhe: 1.585 ± 0.251
5.871ValGly: 5.871 ± 0.519
1.409ValHis: 1.409 ± 0.331
3.816ValIle: 3.816 ± 0.543
2.466ValLys: 2.466 ± 0.404
5.754ValLeu: 5.754 ± 0.487
1.996ValMet: 1.996 ± 0.307
2.231ValAsn: 2.231 ± 0.374
4.99ValPro: 4.99 ± 0.516
2.407ValGln: 2.407 ± 0.356
4.99ValArg: 4.99 ± 0.53
5.108ValSer: 5.108 ± 0.616
6.399ValThr: 6.399 ± 0.628
5.519ValVal: 5.519 ± 0.631
1.703ValTrp: 1.703 ± 0.376
1.115ValTyr: 1.115 ± 0.209
0.0ValXaa: 0.0 ± 0.0
Trp
2.055TrpAla: 2.055 ± 0.317
0.47TrpCys: 0.47 ± 0.144
1.233TrpAsp: 1.233 ± 0.258
1.468TrpGlu: 1.468 ± 0.372
0.763TrpPhe: 0.763 ± 0.223
1.468TrpGly: 1.468 ± 0.306
0.47TrpHis: 0.47 ± 0.162
0.763TrpIle: 0.763 ± 0.205
0.646TrpLys: 0.646 ± 0.183
1.761TrpLeu: 1.761 ± 0.37
0.176TrpMet: 0.176 ± 0.108
0.587TrpAsn: 0.587 ± 0.143
0.763TrpPro: 0.763 ± 0.22
0.822TrpGln: 0.822 ± 0.257
1.409TrpArg: 1.409 ± 0.291
1.703TrpSer: 1.703 ± 0.349
1.292TrpThr: 1.292 ± 0.274
1.35TrpVal: 1.35 ± 0.276
0.881TrpTrp: 0.881 ± 0.295
0.822TrpTyr: 0.822 ± 0.204
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.583TyrAla: 2.583 ± 0.343
0.352TyrCys: 0.352 ± 0.163
2.407TyrAsp: 2.407 ± 0.333
1.35TyrGlu: 1.35 ± 0.28
0.411TyrPhe: 0.411 ± 0.15
2.525TyrGly: 2.525 ± 0.421
0.117TyrHis: 0.117 ± 0.081
0.587TyrIle: 0.587 ± 0.161
0.411TyrLys: 0.411 ± 0.158
1.879TyrLeu: 1.879 ± 0.414
0.881TyrMet: 0.881 ± 0.238
0.411TyrAsn: 0.411 ± 0.153
1.233TyrPro: 1.233 ± 0.269
0.881TyrGln: 0.881 ± 0.23
2.407TyrArg: 2.407 ± 0.365
1.233TyrSer: 1.233 ± 0.277
1.703TyrThr: 1.703 ± 0.259
2.172TyrVal: 2.172 ± 0.376
0.528TyrTrp: 0.528 ± 0.166
0.646TyrTyr: 0.646 ± 0.197
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (17034 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski