Microbacterium phage AvGardian

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dismasvirus; unclassified Dismasvirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3TA24|A0A6M3TA24_9CAUD Uncharacterized protein OS=Microbacterium phage AvGardian OX=2725619 GN=29 PE=4 SV=1
MM1 pKa = 7.58DD2 pKa = 3.08TWFCAFRR9 pKa = 11.84FDD11 pKa = 5.09HH12 pKa = 6.54QDD14 pKa = 3.12QPVVVIDD21 pKa = 3.92GEE23 pKa = 4.74AYY25 pKa = 10.18CQACAPEE32 pKa = 4.72APSKK36 pKa = 10.82EE37 pKa = 4.13GAAA40 pKa = 3.97

Molecular weight: 4.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3SXH7|A0A6M3SXH7_9CAUD Uncharacterized protein OS=Microbacterium phage AvGardian OX=2725619 GN=65 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 4.53KK3 pKa = 10.96HH4 pKa = 6.04PATPRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84LYY14 pKa = 8.11EE15 pKa = 3.38AAIRR19 pKa = 11.84VVDD22 pKa = 5.41LIVYY26 pKa = 9.46AAVFLGGIYY35 pKa = 10.61ALVATPNSVIDD46 pKa = 4.03EE47 pKa = 4.33LQGAEE52 pKa = 3.98WLLGLWAALLLGGGLVGFLGRR73 pKa = 11.84LTRR76 pKa = 11.84YY77 pKa = 8.79WLVEE81 pKa = 3.97NPGTVAAFFGILIYY95 pKa = 10.16FVVLGKK101 pKa = 10.09FAFASVTSSVAVVLVLVAMGFMIRR125 pKa = 11.84RR126 pKa = 11.84WFEE129 pKa = 3.44LQIFGTEE136 pKa = 4.23PNAKK140 pKa = 9.06WKK142 pKa = 10.47VRR144 pKa = 11.84LQLALARR151 pKa = 11.84RR152 pKa = 11.84TANAVSRR159 pKa = 11.84SSS161 pKa = 3.05

Molecular weight: 17.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13065

40

873

192.1

20.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.841 ± 0.348

0.528 ± 0.1

6.024 ± 0.259

6.414 ± 0.241

3.276 ± 0.151

8.603 ± 0.328

1.661 ± 0.177

3.919 ± 0.214

4.095 ± 0.264

8.68 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.141

3.138 ± 0.228

5.335 ± 0.222

3.651 ± 0.209

6.399 ± 0.411

5.404 ± 0.225

6.238 ± 0.286

8.129 ± 0.342

2.036 ± 0.163

2.572 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski