Amino acid dipepetide frequency for Microbacterium phage AvGardian

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.544AlaAla: 14.544 ± 1.332
0.23AlaCys: 0.23 ± 0.126
6.889AlaAsp: 6.889 ± 1.021
7.348AlaGlu: 7.348 ± 0.85
3.445AlaPhe: 3.445 ± 0.525
10.41AlaGly: 10.41 ± 0.79
1.837AlaHis: 1.837 ± 0.427
5.511AlaIle: 5.511 ± 0.705
5.511AlaLys: 5.511 ± 0.796
10.028AlaLeu: 10.028 ± 0.995
2.603AlaMet: 2.603 ± 0.567
3.521AlaAsn: 3.521 ± 0.465
6.43AlaPro: 6.43 ± 0.707
5.129AlaGln: 5.129 ± 0.604
7.272AlaArg: 7.272 ± 0.905
5.511AlaSer: 5.511 ± 0.63
6.277AlaThr: 6.277 ± 0.699
10.181AlaVal: 10.181 ± 0.804
2.679AlaTrp: 2.679 ± 0.423
3.062AlaTyr: 3.062 ± 0.508
0.0AlaXaa: 0.0 ± 0.0
Cys
0.459CysAla: 0.459 ± 0.216
0.077CysCys: 0.077 ± 0.075
0.383CysAsp: 0.383 ± 0.184
0.077CysGlu: 0.077 ± 0.076
0.23CysPhe: 0.23 ± 0.14
0.842CysGly: 0.842 ± 0.255
0.153CysHis: 0.153 ± 0.1
0.077CysIle: 0.077 ± 0.068
0.0CysLys: 0.0 ± 0.0
0.153CysLeu: 0.153 ± 0.105
0.077CysMet: 0.077 ± 0.07
0.23CysAsn: 0.23 ± 0.146
0.919CysPro: 0.919 ± 0.27
0.23CysGln: 0.23 ± 0.139
0.459CysArg: 0.459 ± 0.203
0.383CysSer: 0.383 ± 0.183
0.153CysThr: 0.153 ± 0.095
0.23CysVal: 0.23 ± 0.141
0.0CysTrp: 0.0 ± 0.0
0.153CysTyr: 0.153 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
6.43AspAla: 6.43 ± 0.738
0.153AspCys: 0.153 ± 0.103
3.291AspAsp: 3.291 ± 0.664
3.215AspGlu: 3.215 ± 0.473
2.143AspPhe: 2.143 ± 0.415
6.889AspGly: 6.889 ± 0.893
1.301AspHis: 1.301 ± 0.349
1.531AspIle: 1.531 ± 0.379
1.99AspLys: 1.99 ± 0.339
6.124AspLeu: 6.124 ± 0.707
1.378AspMet: 1.378 ± 0.369
1.378AspAsn: 1.378 ± 0.294
4.746AspPro: 4.746 ± 0.561
1.914AspGln: 1.914 ± 0.331
3.215AspArg: 3.215 ± 0.685
2.985AspSer: 2.985 ± 0.513
3.215AspThr: 3.215 ± 0.486
4.669AspVal: 4.669 ± 0.658
1.148AspTrp: 1.148 ± 0.286
2.526AspTyr: 2.526 ± 0.38
0.0AspXaa: 0.0 ± 0.0
Glu
8.114GluAla: 8.114 ± 0.773
0.153GluCys: 0.153 ± 0.099
4.593GluAsp: 4.593 ± 0.567
4.976GluGlu: 4.976 ± 0.638
1.99GluPhe: 1.99 ± 0.342
4.287GluGly: 4.287 ± 0.615
1.531GluHis: 1.531 ± 0.37
1.607GluIle: 1.607 ± 0.377
1.837GluLys: 1.837 ± 0.367
7.655GluLeu: 7.655 ± 0.713
1.607GluMet: 1.607 ± 0.355
1.531GluAsn: 1.531 ± 0.564
3.138GluPro: 3.138 ± 0.489
2.526GluGln: 2.526 ± 0.434
3.674GluArg: 3.674 ± 0.554
2.067GluSer: 2.067 ± 0.383
3.674GluThr: 3.674 ± 0.419
6.2GluVal: 6.2 ± 0.67
1.531GluTrp: 1.531 ± 0.358
1.454GluTyr: 1.454 ± 0.306
0.0GluXaa: 0.0 ± 0.0
Phe
3.368PheAla: 3.368 ± 0.474
0.153PheCys: 0.153 ± 0.109
2.22PheAsp: 2.22 ± 0.348
1.99PheGlu: 1.99 ± 0.358
0.919PhePhe: 0.919 ± 0.264
2.296PheGly: 2.296 ± 0.421
0.383PheHis: 0.383 ± 0.198
1.072PheIle: 1.072 ± 0.293
2.067PheLys: 2.067 ± 0.33
2.756PheLeu: 2.756 ± 0.519
0.919PheMet: 0.919 ± 0.242
1.148PheAsn: 1.148 ± 0.287
1.225PhePro: 1.225 ± 0.281
1.301PheGln: 1.301 ± 0.311
3.445PheArg: 3.445 ± 0.489
2.22PheSer: 2.22 ± 0.42
2.526PheThr: 2.526 ± 0.434
1.837PheVal: 1.837 ± 0.444
0.459PheTrp: 0.459 ± 0.21
0.459PheTyr: 0.459 ± 0.16
0.0PheXaa: 0.0 ± 0.0
Gly
8.42GlyAla: 8.42 ± 0.938
0.995GlyCys: 0.995 ± 0.307
5.052GlyAsp: 5.052 ± 0.622
5.129GlyGlu: 5.129 ± 0.665
2.909GlyPhe: 2.909 ± 0.565
6.66GlyGly: 6.66 ± 0.759
1.225GlyHis: 1.225 ± 0.363
3.215GlyIle: 3.215 ± 0.465
3.751GlyLys: 3.751 ± 0.54
7.425GlyLeu: 7.425 ± 0.464
2.296GlyMet: 2.296 ± 0.488
2.909GlyAsn: 2.909 ± 0.52
3.521GlyPro: 3.521 ± 0.725
4.21GlyGln: 4.21 ± 0.738
5.052GlyArg: 5.052 ± 0.503
5.052GlySer: 5.052 ± 0.684
6.889GlyThr: 6.889 ± 0.947
6.736GlyVal: 6.736 ± 0.795
1.761GlyTrp: 1.761 ± 0.334
2.832GlyTyr: 2.832 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
1.684HisAla: 1.684 ± 0.39
0.153HisCys: 0.153 ± 0.104
0.536HisAsp: 0.536 ± 0.182
1.072HisGlu: 1.072 ± 0.257
0.842HisPhe: 0.842 ± 0.306
1.225HisGly: 1.225 ± 0.332
0.23HisHis: 0.23 ± 0.126
0.765HisIle: 0.765 ± 0.227
0.689HisLys: 0.689 ± 0.222
1.607HisLeu: 1.607 ± 0.305
0.383HisMet: 0.383 ± 0.171
0.612HisAsn: 0.612 ± 0.208
1.225HisPro: 1.225 ± 0.26
0.383HisGln: 0.383 ± 0.164
1.301HisArg: 1.301 ± 0.305
0.689HisSer: 0.689 ± 0.288
0.765HisThr: 0.765 ± 0.209
1.454HisVal: 1.454 ± 0.346
0.23HisTrp: 0.23 ± 0.135
0.765HisTyr: 0.765 ± 0.219
0.0HisXaa: 0.0 ± 0.0
Ile
3.904IleAla: 3.904 ± 0.484
0.0IleCys: 0.0 ± 0.0
2.756IleAsp: 2.756 ± 0.43
2.449IleGlu: 2.449 ± 0.443
1.072IlePhe: 1.072 ± 0.244
3.751IleGly: 3.751 ± 0.67
1.225IleHis: 1.225 ± 0.282
1.837IleIle: 1.837 ± 0.361
2.449IleLys: 2.449 ± 0.539
3.751IleLeu: 3.751 ± 0.509
0.153IleMet: 0.153 ± 0.094
1.225IleAsn: 1.225 ± 0.295
1.607IlePro: 1.607 ± 0.322
0.765IleGln: 0.765 ± 0.248
2.067IleArg: 2.067 ± 0.41
2.373IleSer: 2.373 ± 0.444
2.985IleThr: 2.985 ± 0.537
3.368IleVal: 3.368 ± 0.495
0.459IleTrp: 0.459 ± 0.189
0.995IleTyr: 0.995 ± 0.293
0.0IleXaa: 0.0 ± 0.0
Lys
6.277LysAla: 6.277 ± 0.96
0.383LysCys: 0.383 ± 0.144
2.22LysAsp: 2.22 ± 0.466
2.296LysGlu: 2.296 ± 0.391
1.072LysPhe: 1.072 ± 0.352
3.598LysGly: 3.598 ± 0.494
0.536LysHis: 0.536 ± 0.171
1.454LysIle: 1.454 ± 0.378
1.684LysLys: 1.684 ± 0.36
3.751LysLeu: 3.751 ± 0.653
0.995LysMet: 0.995 ± 0.221
0.919LysAsn: 0.919 ± 0.248
2.449LysPro: 2.449 ± 0.529
1.761LysGln: 1.761 ± 0.373
2.603LysArg: 2.603 ± 0.374
2.603LysSer: 2.603 ± 0.398
2.296LysThr: 2.296 ± 0.392
3.062LysVal: 3.062 ± 0.514
0.459LysTrp: 0.459 ± 0.188
0.536LysTyr: 0.536 ± 0.198
0.0LysXaa: 0.0 ± 0.0
Leu
10.334LeuAla: 10.334 ± 0.954
0.459LeuCys: 0.459 ± 0.172
5.818LeuAsp: 5.818 ± 0.687
5.282LeuGlu: 5.282 ± 0.749
2.296LeuPhe: 2.296 ± 0.508
8.114LeuGly: 8.114 ± 0.763
1.148LeuHis: 1.148 ± 0.294
3.521LeuIle: 3.521 ± 0.674
3.521LeuLys: 3.521 ± 0.563
7.195LeuLeu: 7.195 ± 0.65
2.603LeuMet: 2.603 ± 0.407
2.679LeuAsn: 2.679 ± 0.457
4.363LeuPro: 4.363 ± 0.593
4.21LeuGln: 4.21 ± 0.625
5.971LeuArg: 5.971 ± 0.791
4.363LeuSer: 4.363 ± 0.499
5.435LeuThr: 5.435 ± 0.563
6.506LeuVal: 6.506 ± 0.775
1.301LeuTrp: 1.301 ± 0.335
1.684LeuTyr: 1.684 ± 0.341
0.0LeuXaa: 0.0 ± 0.0
Met
3.98MetAla: 3.98 ± 0.536
0.0MetCys: 0.0 ± 0.0
1.684MetAsp: 1.684 ± 0.351
0.842MetGlu: 0.842 ± 0.209
0.23MetPhe: 0.23 ± 0.155
1.531MetGly: 1.531 ± 0.348
0.0MetHis: 0.0 ± 0.0
0.612MetIle: 0.612 ± 0.191
0.842MetLys: 0.842 ± 0.289
1.301MetLeu: 1.301 ± 0.286
0.23MetMet: 0.23 ± 0.122
1.301MetAsn: 1.301 ± 0.313
0.919MetPro: 0.919 ± 0.263
0.383MetGln: 0.383 ± 0.159
1.301MetArg: 1.301 ± 0.273
1.454MetSer: 1.454 ± 0.34
1.837MetThr: 1.837 ± 0.352
1.378MetVal: 1.378 ± 0.32
0.383MetTrp: 0.383 ± 0.174
0.383MetTyr: 0.383 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
3.368AsnAla: 3.368 ± 0.56
0.23AsnCys: 0.23 ± 0.118
1.378AsnAsp: 1.378 ± 0.344
1.454AsnGlu: 1.454 ± 0.316
0.842AsnPhe: 0.842 ± 0.29
3.062AsnGly: 3.062 ± 0.485
0.612AsnHis: 0.612 ± 0.226
1.148AsnIle: 1.148 ± 0.277
1.378AsnLys: 1.378 ± 0.34
2.526AsnLeu: 2.526 ± 0.381
0.23AsnMet: 0.23 ± 0.17
1.378AsnAsn: 1.378 ± 0.359
2.373AsnPro: 2.373 ± 0.313
0.842AsnGln: 0.842 ± 0.249
2.22AsnArg: 2.22 ± 0.452
2.679AsnSer: 2.679 ± 0.497
1.607AsnThr: 1.607 ± 0.352
2.143AsnVal: 2.143 ± 0.457
0.842AsnTrp: 0.842 ± 0.271
1.072AsnTyr: 1.072 ± 0.328
0.0AsnXaa: 0.0 ± 0.0
Pro
7.272ProAla: 7.272 ± 0.611
0.23ProCys: 0.23 ± 0.125
3.062ProAsp: 3.062 ± 0.445
5.282ProGlu: 5.282 ± 0.658
2.143ProPhe: 2.143 ± 0.466
4.899ProGly: 4.899 ± 0.577
0.919ProHis: 0.919 ± 0.242
2.067ProIle: 2.067 ± 0.44
1.914ProLys: 1.914 ± 0.463
3.521ProLeu: 3.521 ± 0.515
0.765ProMet: 0.765 ± 0.247
2.143ProAsn: 2.143 ± 0.492
2.373ProPro: 2.373 ± 0.4
1.607ProGln: 1.607 ± 0.355
2.143ProArg: 2.143 ± 0.352
3.368ProSer: 3.368 ± 0.393
3.445ProThr: 3.445 ± 0.433
3.827ProVal: 3.827 ± 0.502
1.531ProTrp: 1.531 ± 0.35
0.842ProTyr: 0.842 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
5.664GlnAla: 5.664 ± 0.636
0.23GlnCys: 0.23 ± 0.149
1.99GlnAsp: 1.99 ± 0.403
2.373GlnGlu: 2.373 ± 0.352
1.454GlnPhe: 1.454 ± 0.405
3.215GlnGly: 3.215 ± 0.519
0.383GlnHis: 0.383 ± 0.156
0.995GlnIle: 0.995 ± 0.214
1.148GlnLys: 1.148 ± 0.325
3.98GlnLeu: 3.98 ± 0.411
0.689GlnMet: 0.689 ± 0.223
0.919GlnAsn: 0.919 ± 0.222
1.531GlnPro: 1.531 ± 0.406
1.225GlnGln: 1.225 ± 0.325
2.067GlnArg: 2.067 ± 0.41
1.454GlnSer: 1.454 ± 0.308
1.761GlnThr: 1.761 ± 0.328
3.751GlnVal: 3.751 ± 0.676
0.612GlnTrp: 0.612 ± 0.268
1.072GlnTyr: 1.072 ± 0.263
0.0GlnXaa: 0.0 ± 0.0
Arg
6.506ArgAla: 6.506 ± 0.879
0.536ArgCys: 0.536 ± 0.236
3.674ArgAsp: 3.674 ± 0.516
4.363ArgGlu: 4.363 ± 0.679
2.22ArgPhe: 2.22 ± 0.432
4.746ArgGly: 4.746 ± 0.634
1.301ArgHis: 1.301 ± 0.337
2.373ArgIle: 2.373 ± 0.417
2.679ArgLys: 2.679 ± 0.474
5.664ArgLeu: 5.664 ± 0.693
0.919ArgMet: 0.919 ± 0.232
1.684ArgAsn: 1.684 ± 0.299
3.674ArgPro: 3.674 ± 0.589
2.373ArgGln: 2.373 ± 0.381
6.43ArgArg: 6.43 ± 0.915
3.598ArgSer: 3.598 ± 0.572
2.832ArgThr: 2.832 ± 0.411
5.588ArgVal: 5.588 ± 0.697
1.454ArgTrp: 1.454 ± 0.321
1.378ArgTyr: 1.378 ± 0.297
0.0ArgXaa: 0.0 ± 0.0
Ser
6.506SerAla: 6.506 ± 0.749
0.306SerCys: 0.306 ± 0.156
3.521SerAsp: 3.521 ± 0.422
2.296SerGlu: 2.296 ± 0.425
2.067SerPhe: 2.067 ± 0.492
5.358SerGly: 5.358 ± 0.644
0.995SerHis: 0.995 ± 0.309
2.756SerIle: 2.756 ± 0.424
2.909SerLys: 2.909 ± 0.414
4.21SerLeu: 4.21 ± 0.562
1.378SerMet: 1.378 ± 0.355
1.837SerAsn: 1.837 ± 0.379
2.373SerPro: 2.373 ± 0.398
1.684SerGln: 1.684 ± 0.393
2.603SerArg: 2.603 ± 0.457
2.909SerSer: 2.909 ± 0.591
3.291SerThr: 3.291 ± 0.579
4.21SerVal: 4.21 ± 0.621
1.454SerTrp: 1.454 ± 0.245
1.301SerTyr: 1.301 ± 0.294
0.0SerXaa: 0.0 ± 0.0
Thr
7.119ThrAla: 7.119 ± 0.841
0.306ThrCys: 0.306 ± 0.193
3.674ThrAsp: 3.674 ± 0.501
3.521ThrGlu: 3.521 ± 0.404
2.449ThrPhe: 2.449 ± 0.453
5.435ThrGly: 5.435 ± 0.661
1.072ThrHis: 1.072 ± 0.331
3.751ThrIle: 3.751 ± 0.602
2.603ThrLys: 2.603 ± 0.476
4.44ThrLeu: 4.44 ± 0.517
0.842ThrMet: 0.842 ± 0.278
1.531ThrAsn: 1.531 ± 0.368
4.133ThrPro: 4.133 ± 0.574
1.148ThrGln: 1.148 ± 0.235
3.598ThrArg: 3.598 ± 0.512
3.674ThrSer: 3.674 ± 0.588
4.133ThrThr: 4.133 ± 0.711
5.741ThrVal: 5.741 ± 0.825
1.531ThrTrp: 1.531 ± 0.282
1.684ThrTyr: 1.684 ± 0.305
0.0ThrXaa: 0.0 ± 0.0
Val
9.951ValAla: 9.951 ± 0.8
0.153ValCys: 0.153 ± 0.117
4.669ValAsp: 4.669 ± 0.65
6.736ValGlu: 6.736 ± 0.666
2.832ValPhe: 2.832 ± 0.524
5.664ValGly: 5.664 ± 0.672
1.301ValHis: 1.301 ± 0.297
3.138ValIle: 3.138 ± 0.437
3.138ValLys: 3.138 ± 0.469
7.195ValLeu: 7.195 ± 0.921
1.301ValMet: 1.301 ± 0.309
2.679ValAsn: 2.679 ± 0.44
4.363ValPro: 4.363 ± 0.791
2.526ValGln: 2.526 ± 0.431
5.511ValArg: 5.511 ± 0.641
3.598ValSer: 3.598 ± 0.502
5.741ValThr: 5.741 ± 0.731
6.583ValVal: 6.583 ± 0.586
1.761ValTrp: 1.761 ± 0.42
2.449ValTyr: 2.449 ± 0.477
0.0ValXaa: 0.0 ± 0.0
Trp
2.22TrpAla: 2.22 ± 0.319
0.077TrpCys: 0.077 ± 0.08
0.919TrpAsp: 0.919 ± 0.272
1.454TrpGlu: 1.454 ± 0.361
0.919TrpPhe: 0.919 ± 0.284
1.454TrpGly: 1.454 ± 0.305
0.153TrpHis: 0.153 ± 0.097
0.919TrpIle: 0.919 ± 0.227
0.765TrpLys: 0.765 ± 0.213
1.914TrpLeu: 1.914 ± 0.396
0.612TrpMet: 0.612 ± 0.172
0.995TrpAsn: 0.995 ± 0.406
0.306TrpPro: 0.306 ± 0.137
1.148TrpGln: 1.148 ± 0.366
0.842TrpArg: 0.842 ± 0.326
1.531TrpSer: 1.531 ± 0.341
1.148TrpThr: 1.148 ± 0.375
2.22TrpVal: 2.22 ± 0.363
0.077TrpTrp: 0.077 ± 0.077
0.689TrpTyr: 0.689 ± 0.239
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.296TyrAla: 2.296 ± 0.438
0.459TyrCys: 0.459 ± 0.199
1.914TyrAsp: 1.914 ± 0.35
1.99TyrGlu: 1.99 ± 0.406
0.689TyrPhe: 0.689 ± 0.249
2.603TyrGly: 2.603 ± 0.451
0.306TyrHis: 0.306 ± 0.138
0.842TyrIle: 0.842 ± 0.271
0.077TyrLys: 0.077 ± 0.064
1.607TyrLeu: 1.607 ± 0.418
0.536TyrMet: 0.536 ± 0.203
0.765TyrAsn: 0.765 ± 0.249
1.684TyrPro: 1.684 ± 0.353
1.148TyrGln: 1.148 ± 0.293
2.22TyrArg: 2.22 ± 0.492
1.531TyrSer: 1.531 ± 0.346
2.373TyrThr: 2.373 ± 0.349
1.607TyrVal: 1.607 ± 0.359
0.689TyrTrp: 0.689 ± 0.237
0.383TyrTyr: 0.383 ± 0.156
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (13065 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski