Nocardia arthritidis
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8970 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G9YR97|A0A6G9YR97_9NOCA Transposase OS=Nocardia arthritidis OX=228602 GN=F5544_39045 PE=4 SV=1
MM1 pKa = 7.61 EE2 pKa = 5.5 NNLPALDD9 pKa = 3.82 INALEE14 pKa = 4.14 ISEE17 pKa = 4.62 FLDD20 pKa = 4.06 DD21 pKa = 5.35 SRR23 pKa = 11.84 MDD25 pKa = 3.84 DD26 pKa = 3.4 RR27 pKa = 11.84 DD28 pKa = 3.61 VVAKK32 pKa = 10.57 VMSASCTTCEE42 pKa = 4.57 CSCSCSSS49 pKa = 3.61
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.846
IPC_protein 3.719
Toseland 3.528
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.452
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.846
Patrickios 0.604
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|A0A6G9Y4E1|A0A6G9Y4E1_9NOCA CBS domain-containing protein OS=Nocardia arthritidis OX=228602 GN=F5544_00155 PE=4 SV=1
MM1 pKa = 7.07 LTVAKK6 pKa = 10.12 GKK8 pKa = 8.71 RR9 pKa = 11.84 TFQPNNRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 ARR20 pKa = 11.84 VHH22 pKa = 5.99 GFRR25 pKa = 11.84 LRR27 pKa = 11.84 MRR29 pKa = 11.84 TRR31 pKa = 11.84 AGRR34 pKa = 11.84 AIVSARR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 KK43 pKa = 10.17 GRR45 pKa = 11.84 GTLTAA50 pKa = 4.78
Molecular weight: 5.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.705
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.427
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.265
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8970
0
8970
3023542
29
14483
337.1
36.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.325 ± 0.041
0.841 ± 0.008
6.107 ± 0.02
5.213 ± 0.026
2.988 ± 0.015
8.875 ± 0.024
2.237 ± 0.013
4.321 ± 0.017
1.94 ± 0.019
10.034 ± 0.031
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.872 ± 0.01
2.137 ± 0.013
6.062 ± 0.024
2.84 ± 0.016
8.118 ± 0.026
5.167 ± 0.017
6.094 ± 0.02
8.187 ± 0.028
1.504 ± 0.01
2.139 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here