Mycobacterium virus Che12
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q1A0D4|Q1A0D4_9CAUD Uncharacterized protein OS=Mycobacterium virus Che12 OX=373405 GN=83 PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 4.2 EE3 pKa = 4.45 VKK5 pKa = 10.59 EE6 pKa = 3.86 ALNNFLEE13 pKa = 4.47 EE14 pKa = 3.85 QHH16 pKa = 6.58 KK17 pKa = 9.62 KK18 pKa = 8.83 EE19 pKa = 4.23 RR20 pKa = 11.84 YY21 pKa = 7.66 IEE23 pKa = 4.25 SVGGDD28 pKa = 3.71 EE29 pKa = 5.88 DD30 pKa = 4.51 EE31 pKa = 4.98 PSCGYY36 pKa = 11.09 ADD38 pKa = 4.1 YY39 pKa = 11.45 DD40 pKa = 4.6 EE41 pKa = 6.37 AIADD45 pKa = 4.08 YY46 pKa = 11.06 NEE48 pKa = 3.91 SLADD52 pKa = 4.22 LGAALADD59 pKa = 4.84 AIHH62 pKa = 5.99 NQLYY66 pKa = 10.7 DD67 pKa = 3.51 KK68 pKa = 10.85 EE69 pKa = 4.27
Molecular weight: 7.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.961
IPC_protein 3.884
Toseland 3.694
ProMoST 3.986
Dawson 3.859
Bjellqvist 4.062
Wikipedia 3.757
Rodwell 3.719
Grimsley 3.605
Solomon 3.846
Lehninger 3.795
Nozaki 3.973
DTASelect 4.139
Thurlkill 3.745
EMBOSS 3.77
Sillero 3.999
Patrickios 1.888
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>tr|Q1A0G2|Q1A0G2_9CAUD Uncharacterized protein OS=Mycobacterium virus Che12 OX=373405 GN=55 PE=4 SV=1
MM1 pKa = 7.66 TSTKK5 pKa = 10.47 LPYY8 pKa = 9.42 LHH10 pKa = 6.99 KK11 pKa = 10.35 NARR14 pKa = 11.84 SRR16 pKa = 11.84 QITSSEE22 pKa = 3.75 VRR24 pKa = 11.84 EE25 pKa = 4.23 VFAEE29 pKa = 4.02 EE30 pKa = 4.19 VTRR33 pKa = 11.84 HH34 pKa = 5.61 LDD36 pKa = 3.16 RR37 pKa = 11.84 RR38 pKa = 11.84 LDD40 pKa = 3.57 RR41 pKa = 11.84 KK42 pKa = 10.73 DD43 pKa = 3.18 YY44 pKa = 10.61 LRR46 pKa = 11.84 RR47 pKa = 11.84 VMPP50 pKa = 4.81
Molecular weight: 6.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.633
IPC_protein 10.555
Toseland 10.628
ProMoST 10.394
Dawson 10.745
Bjellqvist 10.467
Wikipedia 10.979
Rodwell 10.862
Grimsley 10.804
Solomon 10.877
Lehninger 10.847
Nozaki 10.599
DTASelect 10.467
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.672
Patrickios 10.672
IPC_peptide 10.891
IPC2_peptide 9.311
IPC2.peptide.svr19 8.673
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
98
0
98
16156
28
841
164.9
18.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.885 ± 0.391
0.904 ± 0.126
6.282 ± 0.223
6.759 ± 0.267
3.25 ± 0.151
8.09 ± 0.395
2.135 ± 0.169
5.15 ± 0.149
4.809 ± 0.229
8.053 ± 0.367
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.47 ± 0.135
3.466 ± 0.155
5.416 ± 0.284
3.658 ± 0.197
6.258 ± 0.366
5.342 ± 0.209
5.942 ± 0.225
7.18 ± 0.209
1.931 ± 0.141
3.021 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here