Spring viremia of carp virus (Rhabdovirus carpia)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sprivivirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q91DS3|NCAP_SVCV Nucleoprotein OS=Spring viremia of carp virus OX=696863 GN=N PE=3 SV=1
MM1 pKa = 7.47SLHH4 pKa = 6.48SKK6 pKa = 10.52LSEE9 pKa = 4.14SLKK12 pKa = 10.81AYY14 pKa = 10.49ADD16 pKa = 3.53LDD18 pKa = 3.84KK19 pKa = 10.69TVKK22 pKa = 10.43EE23 pKa = 4.03IEE25 pKa = 4.28EE26 pKa = 4.29QVSSMEE32 pKa = 4.0EE33 pKa = 3.94PVPKK37 pKa = 8.94TVKK40 pKa = 10.36YY41 pKa = 8.65VTFEE45 pKa = 4.16EE46 pKa = 4.28NLSEE50 pKa = 4.52EE51 pKa = 4.36EE52 pKa = 4.3WEE54 pKa = 4.37SDD56 pKa = 4.86SGDD59 pKa = 3.9DD60 pKa = 5.7DD61 pKa = 4.62EE62 pKa = 7.84DD63 pKa = 6.23SIDD66 pKa = 4.52DD67 pKa = 4.25SLIPDD72 pKa = 3.93YY73 pKa = 11.01LRR75 pKa = 11.84EE76 pKa = 4.16SSSITVDD83 pKa = 3.94EE84 pKa = 5.01DD85 pKa = 4.33EE86 pKa = 4.87EE87 pKa = 4.2DD88 pKa = 3.3QKK90 pKa = 11.88EE91 pKa = 4.13DD92 pKa = 3.76MEE94 pKa = 4.43EE95 pKa = 3.98HH96 pKa = 6.64LPTVSWEE103 pKa = 4.05EE104 pKa = 4.03EE105 pKa = 3.86PTGIDD110 pKa = 2.72IGFGPGIVMPSVSNHH125 pKa = 5.61EE126 pKa = 4.11GGTYY130 pKa = 9.91VRR132 pKa = 11.84YY133 pKa = 10.16NGLGGVDD140 pKa = 4.38PNCKK144 pKa = 10.08DD145 pKa = 4.62LISKK149 pKa = 7.7MMRR152 pKa = 11.84SLIGQIGNKK161 pKa = 9.32YY162 pKa = 10.51GYY164 pKa = 10.63DD165 pKa = 3.29IDD167 pKa = 5.26LFDD170 pKa = 3.78YY171 pKa = 11.0QGDD174 pKa = 3.78FLEE177 pKa = 4.56VFLPHH182 pKa = 6.85KK183 pKa = 9.94PSKK186 pKa = 10.61EE187 pKa = 3.82DD188 pKa = 3.1VRR190 pKa = 11.84PDD192 pKa = 2.86IRR194 pKa = 11.84IGKK197 pKa = 9.19KK198 pKa = 9.77NEE200 pKa = 3.82EE201 pKa = 4.3GTSKK205 pKa = 10.39QVSKK209 pKa = 10.77PRR211 pKa = 11.84GKK213 pKa = 10.51EE214 pKa = 3.76KK215 pKa = 10.45IVLKK219 pKa = 10.17TGDD222 pKa = 3.28EE223 pKa = 4.34CGRR226 pKa = 11.84FPMNKK231 pKa = 8.37EE232 pKa = 3.59AKK234 pKa = 9.41KK235 pKa = 10.55RR236 pKa = 11.84EE237 pKa = 4.09PEE239 pKa = 4.18GLWEE243 pKa = 4.12VMKK246 pKa = 10.93VLSVQFDD253 pKa = 3.36PWKK256 pKa = 10.41EE257 pKa = 3.96DD258 pKa = 3.66EE259 pKa = 5.12PPLSLTIRR267 pKa = 11.84DD268 pKa = 3.82LFISEE273 pKa = 4.35SEE275 pKa = 4.08FRR277 pKa = 11.84LHH279 pKa = 6.92CNHH282 pKa = 6.13SQTEE286 pKa = 4.08RR287 pKa = 11.84EE288 pKa = 3.94MALVGIKK295 pKa = 10.07LRR297 pKa = 11.84RR298 pKa = 11.84LYY300 pKa = 11.05NKK302 pKa = 10.09LYY304 pKa = 10.24QKK306 pKa = 10.81YY307 pKa = 9.63RR308 pKa = 11.84LL309 pKa = 3.67

Molecular weight: 35.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q91DR9|L_SVCV RNA-directed RNA polymerase L OS=Spring viremia of carp virus OX=696863 GN=L PE=1 SV=1
MM1 pKa = 6.82STLRR5 pKa = 11.84KK6 pKa = 9.99LFGIKK11 pKa = 9.6KK12 pKa = 10.28SKK14 pKa = 8.75GTPPTYY20 pKa = 10.74EE21 pKa = 4.01EE22 pKa = 4.31TLATAPVLMDD32 pKa = 2.99THH34 pKa = 6.8DD35 pKa = 3.75THH37 pKa = 7.47SHH39 pKa = 4.98SLQWMRR45 pKa = 11.84YY46 pKa = 7.96HH47 pKa = 7.71VEE49 pKa = 4.16LDD51 pKa = 3.69VKK53 pKa = 11.05LDD55 pKa = 3.82TPLKK59 pKa = 9.96TMSDD63 pKa = 3.5LLGLLKK69 pKa = 10.79NWDD72 pKa = 3.16VDD74 pKa = 4.11YY75 pKa = 11.22KK76 pKa = 10.99GSRR79 pKa = 11.84NKK81 pKa = 10.08RR82 pKa = 11.84RR83 pKa = 11.84FYY85 pKa = 10.36RR86 pKa = 11.84LIMFRR91 pKa = 11.84CALEE95 pKa = 4.25LKK97 pKa = 9.73HH98 pKa = 7.61VSGTYY103 pKa = 10.34SVDD106 pKa = 3.04GSALYY111 pKa = 10.53SNKK114 pKa = 9.44VQGSCYY120 pKa = 8.93VPHH123 pKa = 7.7RR124 pKa = 11.84FGQMPPFKK132 pKa = 10.6RR133 pKa = 11.84EE134 pKa = 3.71IEE136 pKa = 4.18VFRR139 pKa = 11.84YY140 pKa = 8.67PVHH143 pKa = 4.51QHH145 pKa = 5.98GYY147 pKa = 10.25NGMVDD152 pKa = 4.26LRR154 pKa = 11.84MSICDD159 pKa = 3.82LNGEE163 pKa = 4.34KK164 pKa = 9.87IGLNLLKK171 pKa = 9.71EE172 pKa = 4.53CQVAHH177 pKa = 6.87PNHH180 pKa = 5.61FQKK183 pKa = 11.0YY184 pKa = 8.03LEE186 pKa = 4.23EE187 pKa = 4.94VGLEE191 pKa = 4.13AACSATGEE199 pKa = 4.48WILDD203 pKa = 3.37WTFPMPVDD211 pKa = 3.55VVPRR215 pKa = 11.84VPSLFMGDD223 pKa = 3.19

Molecular weight: 25.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3554

223

2095

710.8

80.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.221 ± 0.544

1.829 ± 0.25

5.684 ± 0.667

6.95 ± 0.856

3.545 ± 0.145

6.781 ± 0.405

2.842 ± 0.314

6.64 ± 0.673

6.303 ± 0.533

9.398 ± 0.9

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.87 ± 0.253

4.192 ± 0.292

4.502 ± 0.299

3.18 ± 0.345

5.402 ± 0.337

8.329 ± 0.627

5.712 ± 0.425

6.078 ± 0.606

1.998 ± 0.12

3.545 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski