Actinoplanes sp. N902-109

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Actinoplanes; unclassified Actinoplanes

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4L265|R4L265_9ACTN Putative ABC transporter OS=Actinoplanes sp. N902-109 OX=649831 GN=L083_0097 PE=4 SV=1
MM1 pKa = 7.63ILNALGDD8 pKa = 3.77NAEE11 pKa = 4.52DD12 pKa = 3.79VAHH15 pKa = 7.23LLQFGGWRR23 pKa = 11.84GLPHH27 pKa = 7.33NINACPVALYY37 pKa = 10.66LRR39 pKa = 11.84TVVTDD44 pKa = 3.34VTDD47 pKa = 3.68AAVSSDD53 pKa = 3.15YY54 pKa = 12.0AMVHH58 pKa = 5.59TSDD61 pKa = 3.59GAEE64 pKa = 3.9VSVDD68 pKa = 3.73LSPAVAGFVLAFDD81 pKa = 4.76LGTYY85 pKa = 9.95PEE87 pKa = 5.29LIEE90 pKa = 5.03TATDD94 pKa = 3.69DD95 pKa = 3.88NGDD98 pKa = 3.86VIDD101 pKa = 5.48DD102 pKa = 4.33LDD104 pKa = 3.51RR105 pKa = 6.36

Molecular weight: 11.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4LTP6|R4LTP6_9ACTN Peptidase c26 OS=Actinoplanes sp. N902-109 OX=649831 GN=L083_6821 PE=4 SV=1
MM1 pKa = 7.34HH2 pKa = 6.53VHH4 pKa = 6.32LAAVRR9 pKa = 11.84AHH11 pKa = 5.43VVRR14 pKa = 11.84AGSGVWGSGVRR25 pKa = 11.84RR26 pKa = 11.84SGIRR30 pKa = 11.84RR31 pKa = 11.84SGVRR35 pKa = 11.84RR36 pKa = 11.84SGSWPRR42 pKa = 11.84LSGGNRR48 pKa = 11.84LSHH51 pKa = 6.57GNQPGG56 pKa = 2.98

Molecular weight: 5.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8211

0

8211

2758869

37

10025

336.0

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.218 ± 0.049

0.71 ± 0.008

6.008 ± 0.021

4.818 ± 0.027

2.702 ± 0.016

9.404 ± 0.034

2.177 ± 0.017

3.42 ± 0.022

1.77 ± 0.019

10.333 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.695 ± 0.01

1.87 ± 0.019

6.205 ± 0.028

2.97 ± 0.017

7.881 ± 0.032

5.09 ± 0.028

6.477 ± 0.036

8.69 ± 0.029

1.541 ± 0.011

2.019 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski