Amino acid dipepetide frequency for Actinoplanes sp. N902-109

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.249AlaAla: 24.249 ± 0.173
0.984AlaCys: 0.984 ± 0.021
8.754AlaAsp: 8.754 ± 0.065
8.018AlaGlu: 8.018 ± 0.077
3.441AlaPhe: 3.441 ± 0.036
15.78AlaGly: 15.78 ± 0.119
2.552AlaHis: 2.552 ± 0.035
4.196AlaIle: 4.196 ± 0.045
2.406AlaLys: 2.406 ± 0.045
14.145AlaLeu: 14.145 ± 0.098
2.625AlaMet: 2.625 ± 0.033
2.222AlaAsn: 2.222 ± 0.033
7.217AlaPro: 7.217 ± 0.09
4.065AlaGln: 4.065 ± 0.047
9.845AlaArg: 9.845 ± 0.082
5.682AlaSer: 5.682 ± 0.059
8.536AlaThr: 8.536 ± 0.068
12.878AlaVal: 12.878 ± 0.106
1.945AlaTrp: 1.945 ± 0.031
2.639AlaTyr: 2.639 ± 0.032
0.0AlaXaa: 0.0 ± 0.0
Cys
0.921CysAla: 0.921 ± 0.02
0.092CysCys: 0.092 ± 0.007
0.443CysAsp: 0.443 ± 0.015
0.286CysGlu: 0.286 ± 0.011
0.198CysPhe: 0.198 ± 0.009
0.812CysGly: 0.812 ± 0.021
0.167CysHis: 0.167 ± 0.007
0.158CysIle: 0.158 ± 0.008
0.104CysLys: 0.104 ± 0.008
0.673CysLeu: 0.673 ± 0.019
0.108CysMet: 0.108 ± 0.006
0.132CysAsn: 0.132 ± 0.008
0.45CysPro: 0.45 ± 0.015
0.166CysGln: 0.166 ± 0.008
0.63CysArg: 0.63 ± 0.018
0.475CysSer: 0.475 ± 0.015
0.486CysThr: 0.486 ± 0.02
0.541CysVal: 0.541 ± 0.014
0.111CysTrp: 0.111 ± 0.007
0.151CysTyr: 0.151 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.643AspAla: 7.643 ± 0.065
0.376AspCys: 0.376 ± 0.014
3.911AspAsp: 3.911 ± 0.046
3.576AspGlu: 3.576 ± 0.044
1.493AspPhe: 1.493 ± 0.029
5.956AspGly: 5.956 ± 0.059
1.323AspHis: 1.323 ± 0.025
1.911AspIle: 1.911 ± 0.03
1.103AspLys: 1.103 ± 0.028
6.574AspLeu: 6.574 ± 0.054
0.763AspMet: 0.763 ± 0.018
1.144AspAsn: 1.144 ± 0.025
4.737AspPro: 4.737 ± 0.043
1.768AspGln: 1.768 ± 0.03
5.097AspArg: 5.097 ± 0.049
2.458AspSer: 2.458 ± 0.033
3.004AspThr: 3.004 ± 0.036
5.031AspVal: 5.031 ± 0.047
0.995AspTrp: 0.995 ± 0.023
1.219AspTyr: 1.219 ± 0.022
0.0AspXaa: 0.0 ± 0.0
Glu
6.152GluAla: 6.152 ± 0.057
0.32GluCys: 0.32 ± 0.013
2.154GluAsp: 2.154 ± 0.029
2.229GluGlu: 2.229 ± 0.033
1.401GluPhe: 1.401 ± 0.025
3.018GluGly: 3.018 ± 0.037
1.423GluHis: 1.423 ± 0.025
2.126GluIle: 2.126 ± 0.032
0.992GluLys: 0.992 ± 0.024
6.271GluLeu: 6.271 ± 0.057
0.818GluMet: 0.818 ± 0.018
0.824GluAsn: 0.824 ± 0.019
3.321GluPro: 3.321 ± 0.046
2.342GluGln: 2.342 ± 0.03
4.472GluArg: 4.472 ± 0.047
2.105GluSer: 2.105 ± 0.03
2.508GluThr: 2.508 ± 0.034
4.131GluVal: 4.131 ± 0.042
0.658GluTrp: 0.658 ± 0.015
0.917GluTyr: 0.917 ± 0.02
0.0GluXaa: 0.0 ± 0.0
Phe
3.818PheAla: 3.818 ± 0.038
0.243PheCys: 0.243 ± 0.009
2.045PheAsp: 2.045 ± 0.029
1.253PheGlu: 1.253 ± 0.021
0.862PhePhe: 0.862 ± 0.02
2.958PheGly: 2.958 ± 0.043
0.575PheHis: 0.575 ± 0.015
0.729PheIle: 0.729 ± 0.02
0.428PheLys: 0.428 ± 0.014
2.484PheLeu: 2.484 ± 0.039
0.331PheMet: 0.331 ± 0.011
0.519PheAsn: 0.519 ± 0.014
1.432PhePro: 1.432 ± 0.026
0.723PheGln: 0.723 ± 0.018
1.828PheArg: 1.828 ± 0.028
1.462PheSer: 1.462 ± 0.027
1.973PheThr: 1.973 ± 0.029
2.355PheVal: 2.355 ± 0.034
0.427PheTrp: 0.427 ± 0.015
0.578PheTyr: 0.578 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
11.156GlyAla: 11.156 ± 0.088
0.804GlyCys: 0.804 ± 0.02
5.298GlyAsp: 5.298 ± 0.051
4.34GlyGlu: 4.34 ± 0.046
2.946GlyPhe: 2.946 ± 0.037
8.514GlyGly: 8.514 ± 0.083
2.267GlyHis: 2.267 ± 0.034
3.487GlyIle: 3.487 ± 0.043
2.148GlyLys: 2.148 ± 0.039
9.457GlyLeu: 9.457 ± 0.076
1.959GlyMet: 1.959 ± 0.031
1.939GlyAsn: 1.939 ± 0.029
5.075GlyPro: 5.075 ± 0.055
2.804GlyGln: 2.804 ± 0.038
7.511GlyArg: 7.511 ± 0.071
5.517GlySer: 5.517 ± 0.054
6.752GlyThr: 6.752 ± 0.062
7.81GlyVal: 7.81 ± 0.065
1.848GlyTrp: 1.848 ± 0.031
2.413GlyTyr: 2.413 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.565HisAla: 2.565 ± 0.039
0.164HisCys: 0.164 ± 0.008
1.428HisAsp: 1.428 ± 0.027
1.11HisGlu: 1.11 ± 0.019
0.572HisPhe: 0.572 ± 0.014
2.094HisGly: 2.094 ± 0.032
0.641HisHis: 0.641 ± 0.019
0.616HisIle: 0.616 ± 0.014
0.286HisLys: 0.286 ± 0.01
2.429HisLeu: 2.429 ± 0.034
0.281HisMet: 0.281 ± 0.01
0.388HisAsn: 0.388 ± 0.012
1.815HisPro: 1.815 ± 0.031
0.678HisGln: 0.678 ± 0.016
2.126HisArg: 2.126 ± 0.035
0.983HisSer: 0.983 ± 0.022
1.181HisThr: 1.181 ± 0.021
1.657HisVal: 1.657 ± 0.028
0.312HisTrp: 0.312 ± 0.011
0.442HisTyr: 0.442 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
5.264IleAla: 5.264 ± 0.056
0.277IleCys: 0.277 ± 0.01
2.553IleAsp: 2.553 ± 0.032
2.007IleGlu: 2.007 ± 0.029
0.829IlePhe: 0.829 ± 0.018
3.699IleGly: 3.699 ± 0.039
0.602IleHis: 0.602 ± 0.014
1.059IleIle: 1.059 ± 0.02
0.723IleLys: 0.723 ± 0.018
2.563IleLeu: 2.563 ± 0.039
0.492IleMet: 0.492 ± 0.017
0.833IleAsn: 0.833 ± 0.019
1.732IlePro: 1.732 ± 0.027
0.756IleGln: 0.756 ± 0.017
2.388IleArg: 2.388 ± 0.029
1.885IleSer: 1.885 ± 0.026
2.505IleThr: 2.505 ± 0.031
3.053IleVal: 3.053 ± 0.038
0.412IleTrp: 0.412 ± 0.013
0.572IleTyr: 0.572 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.356LysAla: 2.356 ± 0.043
0.112LysCys: 0.112 ± 0.007
0.946LysAsp: 0.946 ± 0.025
0.749LysGlu: 0.749 ± 0.02
0.414LysPhe: 0.414 ± 0.015
1.283LysGly: 1.283 ± 0.027
0.372LysHis: 0.372 ± 0.012
0.787LysIle: 0.787 ± 0.021
0.561LysLys: 0.561 ± 0.02
1.95LysLeu: 1.95 ± 0.035
0.333LysMet: 0.333 ± 0.012
0.433LysAsn: 0.433 ± 0.015
1.261LysPro: 1.261 ± 0.03
0.638LysGln: 0.638 ± 0.018
1.204LysArg: 1.204 ± 0.028
0.94LysSer: 0.94 ± 0.019
1.086LysThr: 1.086 ± 0.026
1.649LysVal: 1.649 ± 0.033
0.234LysTrp: 0.234 ± 0.009
0.39LysTyr: 0.39 ± 0.012
0.0LysXaa: 0.0 ± 0.0
Leu
15.686LeuAla: 15.686 ± 0.108
0.682LeuCys: 0.682 ± 0.017
6.673LeuAsp: 6.673 ± 0.055
3.861LeuGlu: 3.861 ± 0.042
2.569LeuPhe: 2.569 ± 0.039
9.131LeuGly: 9.131 ± 0.058
2.347LeuHis: 2.347 ± 0.033
3.798LeuIle: 3.798 ± 0.049
1.528LeuLys: 1.528 ± 0.027
11.209LeuLeu: 11.209 ± 0.092
1.489LeuMet: 1.489 ± 0.027
1.72LeuAsn: 1.72 ± 0.027
6.506LeuPro: 6.506 ± 0.052
2.418LeuGln: 2.418 ± 0.03
8.779LeuArg: 8.779 ± 0.061
5.583LeuSer: 5.583 ± 0.054
7.57LeuThr: 7.57 ± 0.065
8.873LeuVal: 8.873 ± 0.067
1.293LeuTrp: 1.293 ± 0.024
1.611LeuTyr: 1.611 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.244MetAla: 2.244 ± 0.029
0.104MetCys: 0.104 ± 0.006
0.806MetAsp: 0.806 ± 0.018
0.531MetGlu: 0.531 ± 0.015
0.489MetPhe: 0.489 ± 0.014
1.158MetGly: 1.158 ± 0.024
0.341MetHis: 0.341 ± 0.01
0.727MetIle: 0.727 ± 0.021
0.331MetLys: 0.331 ± 0.011
1.845MetLeu: 1.845 ± 0.028
0.315MetMet: 0.315 ± 0.013
0.356MetAsn: 0.356 ± 0.012
1.127MetPro: 1.127 ± 0.022
0.495MetGln: 0.495 ± 0.014
1.355MetArg: 1.355 ± 0.024
1.222MetSer: 1.222 ± 0.02
1.62MetThr: 1.62 ± 0.026
1.364MetVal: 1.364 ± 0.023
0.205MetTrp: 0.205 ± 0.01
0.316MetTyr: 0.316 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.383AsnAla: 2.383 ± 0.033
0.153AsnCys: 0.153 ± 0.007
1.05AsnAsp: 1.05 ± 0.024
0.773AsnGlu: 0.773 ± 0.018
0.466AsnPhe: 0.466 ± 0.015
2.173AsnGly: 2.173 ± 0.036
0.388AsnHis: 0.388 ± 0.011
0.63AsnIle: 0.63 ± 0.017
0.35AsnLys: 0.35 ± 0.012
1.776AsnLeu: 1.776 ± 0.028
0.286AsnMet: 0.286 ± 0.013
0.513AsnAsn: 0.513 ± 0.021
1.498AsnPro: 1.498 ± 0.028
0.629AsnGln: 0.629 ± 0.02
1.446AsnArg: 1.446 ± 0.022
0.927AsnSer: 0.927 ± 0.021
1.117AsnThr: 1.117 ± 0.024
1.399AsnVal: 1.399 ± 0.025
0.309AsnTrp: 0.309 ± 0.012
0.432AsnTyr: 0.432 ± 0.012
0.0AsnXaa: 0.0 ± 0.0
Pro
10.563ProAla: 10.563 ± 0.098
0.278ProCys: 0.278 ± 0.011
4.479ProAsp: 4.479 ± 0.045
3.57ProGlu: 3.57 ± 0.038
1.468ProPhe: 1.468 ± 0.026
7.141ProGly: 7.141 ± 0.068
1.209ProHis: 1.209 ± 0.022
1.655ProIle: 1.655 ± 0.024
0.856ProLys: 0.856 ± 0.023
5.081ProLeu: 5.081 ± 0.051
1.021ProMet: 1.021 ± 0.019
0.898ProAsn: 0.898 ± 0.023
3.794ProPro: 3.794 ± 0.064
1.878ProGln: 1.878 ± 0.035
3.821ProArg: 3.821 ± 0.047
3.106ProSer: 3.106 ± 0.043
3.277ProThr: 3.277 ± 0.052
5.816ProVal: 5.816 ± 0.055
0.899ProTrp: 0.899 ± 0.02
1.241ProTyr: 1.241 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
4.227GlnAla: 4.227 ± 0.046
0.158GlnCys: 0.158 ± 0.008
1.344GlnAsp: 1.344 ± 0.023
1.179GlnGlu: 1.179 ± 0.022
0.786GlnPhe: 0.786 ± 0.017
2.052GlnGly: 2.052 ± 0.033
0.803GlnHis: 0.803 ± 0.017
1.169GlnIle: 1.169 ± 0.025
0.509GlnLys: 0.509 ± 0.018
3.421GlnLeu: 3.421 ± 0.036
0.487GlnMet: 0.487 ± 0.015
0.566GlnAsn: 0.566 ± 0.016
2.18GlnPro: 2.18 ± 0.038
1.448GlnGln: 1.448 ± 0.027
2.75GlnArg: 2.75 ± 0.032
1.301GlnSer: 1.301 ± 0.027
1.521GlnThr: 1.521 ± 0.028
2.651GlnVal: 2.651 ± 0.034
0.555GlnTrp: 0.555 ± 0.017
0.597GlnTyr: 0.597 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
9.692ArgAla: 9.692 ± 0.082
0.575ArgCys: 0.575 ± 0.019
4.4ArgAsp: 4.4 ± 0.048
3.909ArgGlu: 3.909 ± 0.043
2.284ArgPhe: 2.284 ± 0.03
5.544ArgGly: 5.544 ± 0.05
2.078ArgHis: 2.078 ± 0.029
3.173ArgIle: 3.173 ± 0.04
1.371ArgLys: 1.371 ± 0.031
8.827ArgLeu: 8.827 ± 0.075
1.751ArgMet: 1.751 ± 0.028
1.489ArgAsn: 1.489 ± 0.026
4.961ArgPro: 4.961 ± 0.056
2.45ArgGln: 2.45 ± 0.033
7.849ArgArg: 7.849 ± 0.085
4.222ArgSer: 4.222 ± 0.044
5.166ArgThr: 5.166 ± 0.05
5.846ArgVal: 5.846 ± 0.053
1.44ArgTrp: 1.44 ± 0.028
1.783ArgTyr: 1.783 ± 0.025
0.0ArgXaa: 0.0 ± 0.0
Ser
7.047SerAla: 7.047 ± 0.071
0.398SerCys: 0.398 ± 0.015
2.654SerAsp: 2.654 ± 0.04
1.967SerGlu: 1.967 ± 0.03
1.566SerPhe: 1.566 ± 0.025
5.775SerGly: 5.775 ± 0.067
0.959SerHis: 0.959 ± 0.019
1.718SerIle: 1.718 ± 0.025
0.859SerLys: 0.859 ± 0.021
4.621SerLeu: 4.621 ± 0.048
1.095SerMet: 1.095 ± 0.022
0.977SerAsn: 0.977 ± 0.021
3.265SerPro: 3.265 ± 0.049
1.332SerGln: 1.332 ± 0.026
3.73SerArg: 3.73 ± 0.045
3.169SerSer: 3.169 ± 0.044
3.296SerThr: 3.296 ± 0.046
4.164SerVal: 4.164 ± 0.044
1.025SerTrp: 1.025 ± 0.02
1.287SerTyr: 1.287 ± 0.024
0.0SerXaa: 0.0 ± 0.0
Thr
9.57ThrAla: 9.57 ± 0.085
0.443ThrCys: 0.443 ± 0.014
3.686ThrAsp: 3.686 ± 0.043
2.919ThrGlu: 2.919 ± 0.035
1.822ThrPhe: 1.822 ± 0.031
7.494ThrGly: 7.494 ± 0.077
1.065ThrHis: 1.065 ± 0.02
2.204ThrIle: 2.204 ± 0.037
1.076ThrLys: 1.076 ± 0.029
5.868ThrLeu: 5.868 ± 0.054
1.056ThrMet: 1.056 ± 0.022
1.172ThrAsn: 1.172 ± 0.025
4.282ThrPro: 4.282 ± 0.049
1.41ThrGln: 1.41 ± 0.024
3.944ThrArg: 3.944 ± 0.04
3.356ThrSer: 3.356 ± 0.044
4.47ThrThr: 4.47 ± 0.066
6.548ThrVal: 6.548 ± 0.064
0.97ThrTrp: 0.97 ± 0.023
1.417ThrTyr: 1.417 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
12.096ValAla: 12.096 ± 0.081
0.635ValCys: 0.635 ± 0.018
5.278ValAsp: 5.278 ± 0.062
4.242ValGlu: 4.242 ± 0.049
2.321ValPhe: 2.321 ± 0.034
6.351ValGly: 6.351 ± 0.059
1.841ValHis: 1.841 ± 0.031
3.074ValIle: 3.074 ± 0.046
1.463ValLys: 1.463 ± 0.028
10.087ValLeu: 10.087 ± 0.082
1.261ValMet: 1.261 ± 0.026
1.742ValAsn: 1.742 ± 0.027
5.382ValPro: 5.382 ± 0.049
2.293ValGln: 2.293 ± 0.028
6.945ValArg: 6.945 ± 0.059
4.559ValSer: 4.559 ± 0.056
6.402ValThr: 6.402 ± 0.072
8.473ValVal: 8.473 ± 0.078
1.059ValTrp: 1.059 ± 0.021
1.394ValTyr: 1.394 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.718TrpAla: 1.718 ± 0.024
0.14TrpCys: 0.14 ± 0.009
0.771TrpAsp: 0.771 ± 0.018
0.626TrpGlu: 0.626 ± 0.018
0.474TrpPhe: 0.474 ± 0.015
1.022TrpGly: 1.022 ± 0.021
0.408TrpHis: 0.408 ± 0.014
0.559TrpIle: 0.559 ± 0.015
0.281TrpLys: 0.281 ± 0.011
1.89TrpLeu: 1.89 ± 0.031
0.285TrpMet: 0.285 ± 0.01
0.411TrpAsn: 0.411 ± 0.014
0.872TrpPro: 0.872 ± 0.019
0.719TrpGln: 0.719 ± 0.019
1.369TrpArg: 1.369 ± 0.025
1.036TrpSer: 1.036 ± 0.02
1.082TrpThr: 1.082 ± 0.022
1.037TrpVal: 1.037 ± 0.023
0.365TrpTrp: 0.365 ± 0.012
0.347TrpTyr: 0.347 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.828TyrAla: 2.828 ± 0.036
0.165TyrCys: 0.165 ± 0.008
1.41TyrAsp: 1.41 ± 0.03
1.037TyrGlu: 1.037 ± 0.021
0.622TyrPhe: 0.622 ± 0.018
2.089TyrGly: 2.089 ± 0.03
0.406TyrHis: 0.406 ± 0.012
0.43TyrIle: 0.43 ± 0.017
0.323TyrLys: 0.323 ± 0.014
2.155TyrLeu: 2.155 ± 0.031
0.193TyrMet: 0.193 ± 0.01
0.419TyrAsn: 0.419 ± 0.015
1.146TyrPro: 1.146 ± 0.022
0.692TyrGln: 0.692 ± 0.018
1.721TyrArg: 1.721 ± 0.034
0.917TyrSer: 0.917 ± 0.021
1.22TyrThr: 1.22 ± 0.027
1.622TyrVal: 1.622 ± 0.025
0.351TyrTrp: 0.351 ± 0.012
0.444TyrTyr: 0.444 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8211 proteins (2758869 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski