Piscirickettsia salmonis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Piscirickettsia

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2947 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095BRR8|A0A095BRR8_PISSA 30S ribosomal protein S18 OS=Piscirickettsia salmonis OX=1238 GN=rpsR PE=3 SV=1
MM1 pKa = 7.79TDD3 pKa = 3.18TVNLDD8 pKa = 3.86DD9 pKa = 5.16EE10 pKa = 5.27FKK12 pKa = 11.13QSIQDD17 pKa = 3.42WAYY20 pKa = 10.67GIADD24 pKa = 4.12VLDD27 pKa = 3.68EE28 pKa = 4.27CQRR31 pKa = 11.84NITEE35 pKa = 4.1YY36 pKa = 10.94SDD38 pKa = 5.48EE39 pKa = 4.03EE40 pKa = 4.21FQYY43 pKa = 9.86LTQCLIEE50 pKa = 4.77CDD52 pKa = 3.3QAGFAKK58 pKa = 10.46LMAEE62 pKa = 4.4MRR64 pKa = 11.84FEE66 pKa = 4.51SNKK69 pKa = 9.21QQVNN73 pKa = 3.32

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095E249|A0A095E249_PISSA Cold shock protein CspA OS=Piscirickettsia salmonis OX=1238 GN=cspA PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84LVIKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2947

0

2947

831806

29

1992

282.3

31.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.186 ± 0.045

1.236 ± 0.02

4.934 ± 0.037

5.733 ± 0.055

4.181 ± 0.037

6.089 ± 0.053

2.648 ± 0.028

7.062 ± 0.049

6.234 ± 0.062

10.725 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.023

4.641 ± 0.037

3.632 ± 0.029

5.044 ± 0.046

4.551 ± 0.04

6.769 ± 0.044

5.263 ± 0.037

6.221 ± 0.048

1.064 ± 0.017

3.473 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski