Amino acid dipepetide frequency for Piscirickettsia salmonis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.351AlaAla: 7.351 ± 0.139
1.057AlaCys: 1.057 ± 0.033
3.848AlaAsp: 3.848 ± 0.073
5.017AlaGlu: 5.017 ± 0.121
3.063AlaPhe: 3.063 ± 0.06
5.475AlaGly: 5.475 ± 0.11
1.921AlaHis: 1.921 ± 0.05
5.825AlaIle: 5.825 ± 0.102
5.293AlaLys: 5.293 ± 0.099
9.844AlaLeu: 9.844 ± 0.146
2.166AlaMet: 2.166 ± 0.055
3.193AlaAsn: 3.193 ± 0.076
2.48AlaPro: 2.48 ± 0.068
3.706AlaGln: 3.706 ± 0.071
3.622AlaArg: 3.622 ± 0.09
5.023AlaSer: 5.023 ± 0.089
4.316AlaThr: 4.316 ± 0.084
5.438AlaVal: 5.438 ± 0.093
0.804AlaTrp: 0.804 ± 0.029
2.42AlaTyr: 2.42 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.924CysAla: 0.924 ± 0.031
0.215CysCys: 0.215 ± 0.018
0.632CysAsp: 0.632 ± 0.031
0.65CysGlu: 0.65 ± 0.029
0.618CysPhe: 0.618 ± 0.029
0.811CysGly: 0.811 ± 0.034
0.397CysHis: 0.397 ± 0.022
0.84CysIle: 0.84 ± 0.033
0.689CysLys: 0.689 ± 0.031
1.414CysLeu: 1.414 ± 0.04
0.273CysMet: 0.273 ± 0.02
0.53CysAsn: 0.53 ± 0.027
0.474CysPro: 0.474 ± 0.03
0.714CysGln: 0.714 ± 0.025
0.495CysArg: 0.495 ± 0.022
0.875CysSer: 0.875 ± 0.037
0.463CysThr: 0.463 ± 0.022
0.626CysVal: 0.626 ± 0.027
0.185CysTrp: 0.185 ± 0.015
0.534CysTyr: 0.534 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.302AspAla: 3.302 ± 0.074
0.643AspCys: 0.643 ± 0.035
2.792AspAsp: 2.792 ± 0.086
3.247AspGlu: 3.247 ± 0.074
2.329AspPhe: 2.329 ± 0.061
2.695AspGly: 2.695 ± 0.065
1.405AspHis: 1.405 ± 0.043
3.745AspIle: 3.745 ± 0.075
3.108AspLys: 3.108 ± 0.07
5.337AspLeu: 5.337 ± 0.087
0.998AspMet: 0.998 ± 0.036
2.14AspAsn: 2.14 ± 0.055
1.718AspPro: 1.718 ± 0.05
2.679AspGln: 2.679 ± 0.061
2.051AspArg: 2.051 ± 0.063
2.903AspSer: 2.903 ± 0.068
2.184AspThr: 2.184 ± 0.045
3.152AspVal: 3.152 ± 0.076
0.644AspTrp: 0.644 ± 0.026
2.264AspTyr: 2.264 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
4.457GluAla: 4.457 ± 0.109
0.67GluCys: 0.67 ± 0.03
2.45GluAsp: 2.45 ± 0.064
3.611GluGlu: 3.611 ± 0.118
2.201GluPhe: 2.201 ± 0.054
3.072GluGly: 3.072 ± 0.066
1.791GluHis: 1.791 ± 0.053
3.959GluIle: 3.959 ± 0.068
4.434GluLys: 4.434 ± 0.1
6.375GluLeu: 6.375 ± 0.096
1.249GluMet: 1.249 ± 0.045
2.712GluAsn: 2.712 ± 0.059
1.554GluPro: 1.554 ± 0.046
3.426GluGln: 3.426 ± 0.085
3.17GluArg: 3.17 ± 0.072
3.401GluSer: 3.401 ± 0.077
2.784GluThr: 2.784 ± 0.078
3.728GluVal: 3.728 ± 0.097
0.469GluTrp: 0.469 ± 0.025
1.814GluTyr: 1.814 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.419PheAla: 3.419 ± 0.082
0.665PheCys: 0.665 ± 0.03
2.296PheAsp: 2.296 ± 0.057
2.135PheGlu: 2.135 ± 0.057
2.105PhePhe: 2.105 ± 0.058
2.669PheGly: 2.669 ± 0.061
0.939PheHis: 0.939 ± 0.037
3.557PheIle: 3.557 ± 0.074
2.574PheLys: 2.574 ± 0.058
3.962PheLeu: 3.962 ± 0.085
0.911PheMet: 0.911 ± 0.036
2.366PheAsn: 2.366 ± 0.061
1.285PhePro: 1.285 ± 0.038
1.331PheGln: 1.331 ± 0.043
1.5PheArg: 1.5 ± 0.04
3.666PheSer: 3.666 ± 0.076
2.309PheThr: 2.309 ± 0.053
2.079PheVal: 2.079 ± 0.057
0.445PheTrp: 0.445 ± 0.024
1.597PheTyr: 1.597 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
4.524GlyAla: 4.524 ± 0.111
0.839GlyCys: 0.839 ± 0.031
2.909GlyAsp: 2.909 ± 0.077
3.401GlyGlu: 3.401 ± 0.084
2.949GlyPhe: 2.949 ± 0.063
4.008GlyGly: 4.008 ± 0.108
1.653GlyHis: 1.653 ± 0.046
4.393GlyIle: 4.393 ± 0.084
3.578GlyLys: 3.578 ± 0.083
6.729GlyLeu: 6.729 ± 0.11
1.459GlyMet: 1.459 ± 0.044
2.178GlyAsn: 2.178 ± 0.062
1.601GlyPro: 1.601 ± 0.054
3.08GlyGln: 3.08 ± 0.067
2.931GlyArg: 2.931 ± 0.067
3.866GlySer: 3.866 ± 0.076
2.848GlyThr: 2.848 ± 0.067
4.716GlyVal: 4.716 ± 0.095
0.808GlyTrp: 0.808 ± 0.032
2.415GlyTyr: 2.415 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
2.092HisAla: 2.092 ± 0.053
0.376HisCys: 0.376 ± 0.025
1.151HisAsp: 1.151 ± 0.039
1.271HisGlu: 1.271 ± 0.041
1.386HisPhe: 1.386 ± 0.04
1.521HisGly: 1.521 ± 0.046
0.963HisHis: 0.963 ± 0.04
1.897HisIle: 1.897 ± 0.047
1.288HisLys: 1.288 ± 0.038
2.666HisLeu: 2.666 ± 0.062
0.563HisMet: 0.563 ± 0.026
1.023HisAsn: 1.023 ± 0.039
1.138HisPro: 1.138 ± 0.039
1.528HisGln: 1.528 ± 0.046
1.137HisArg: 1.137 ± 0.042
1.744HisSer: 1.744 ± 0.052
1.422HisThr: 1.422 ± 0.051
1.498HisVal: 1.498 ± 0.048
0.444HisTrp: 0.444 ± 0.025
1.372HisTyr: 1.372 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
6.419IleAla: 6.419 ± 0.108
0.891IleCys: 0.891 ± 0.03
4.448IleAsp: 4.448 ± 0.078
4.442IleGlu: 4.442 ± 0.083
2.93IlePhe: 2.93 ± 0.074
4.322IleGly: 4.322 ± 0.091
1.622IleHis: 1.622 ± 0.049
5.106IleIle: 5.106 ± 0.103
4.584IleLys: 4.584 ± 0.096
6.31IleLeu: 6.31 ± 0.101
1.338IleMet: 1.338 ± 0.041
4.289IleAsn: 4.289 ± 0.097
2.563IlePro: 2.563 ± 0.059
2.777IleGln: 2.777 ± 0.062
2.855IleArg: 2.855 ± 0.061
5.054IleSer: 5.054 ± 0.077
4.094IleThr: 4.094 ± 0.081
3.877IleVal: 3.877 ± 0.079
0.484IleTrp: 0.484 ± 0.023
2.214IleTyr: 2.214 ± 0.053
0.0IleXaa: 0.0 ± 0.0
Lys
4.916LysAla: 4.916 ± 0.099
0.505LysCys: 0.505 ± 0.03
2.876LysAsp: 2.876 ± 0.063
4.05LysGlu: 4.05 ± 0.083
1.839LysPhe: 1.839 ± 0.053
3.076LysGly: 3.076 ± 0.063
1.641LysHis: 1.641 ± 0.044
4.567LysIle: 4.567 ± 0.087
5.077LysLys: 5.077 ± 0.1
6.022LysLeu: 6.022 ± 0.099
1.47LysMet: 1.47 ± 0.044
3.663LysAsn: 3.663 ± 0.079
2.198LysPro: 2.198 ± 0.051
3.336LysGln: 3.336 ± 0.066
3.32LysArg: 3.32 ± 0.075
4.084LysSer: 4.084 ± 0.094
3.611LysThr: 3.611 ± 0.083
3.917LysVal: 3.917 ± 0.09
0.447LysTrp: 0.447 ± 0.032
1.72LysTyr: 1.72 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
9.661LeuAla: 9.661 ± 0.137
1.309LeuCys: 1.309 ± 0.042
5.344LeuAsp: 5.344 ± 0.097
6.052LeuGlu: 6.052 ± 0.102
4.513LeuPhe: 4.513 ± 0.107
6.405LeuGly: 6.405 ± 0.109
2.283LeuHis: 2.283 ± 0.05
7.639LeuIle: 7.639 ± 0.111
7.056LeuLys: 7.056 ± 0.124
10.827LeuLeu: 10.827 ± 0.163
2.561LeuMet: 2.561 ± 0.057
5.392LeuAsn: 5.392 ± 0.097
4.372LeuPro: 4.372 ± 0.068
4.481LeuGln: 4.481 ± 0.081
4.451LeuArg: 4.451 ± 0.079
8.401LeuSer: 8.401 ± 0.107
6.1LeuThr: 6.1 ± 0.083
6.016LeuVal: 6.016 ± 0.101
1.126LeuTrp: 1.126 ± 0.041
3.258LeuTyr: 3.258 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
2.019MetAla: 2.019 ± 0.051
0.226MetCys: 0.226 ± 0.017
0.998MetAsp: 0.998 ± 0.034
0.891MetGlu: 0.891 ± 0.036
0.674MetPhe: 0.674 ± 0.033
1.425MetGly: 1.425 ± 0.046
0.509MetHis: 0.509 ± 0.026
1.629MetIle: 1.629 ± 0.047
1.557MetLys: 1.557 ± 0.041
2.477MetLeu: 2.477 ± 0.056
0.769MetMet: 0.769 ± 0.036
1.12MetAsn: 1.12 ± 0.037
1.209MetPro: 1.209 ± 0.037
1.262MetGln: 1.262 ± 0.043
1.151MetArg: 1.151 ± 0.036
1.717MetSer: 1.717 ± 0.051
1.384MetThr: 1.384 ± 0.045
1.473MetVal: 1.473 ± 0.042
0.159MetTrp: 0.159 ± 0.013
0.51MetTyr: 0.51 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.093AsnAla: 3.093 ± 0.065
0.486AsnCys: 0.486 ± 0.022
2.181AsnAsp: 2.181 ± 0.056
2.259AsnGlu: 2.259 ± 0.06
1.865AsnPhe: 1.865 ± 0.05
2.19AsnGly: 2.19 ± 0.054
1.599AsnHis: 1.599 ± 0.053
3.828AsnIle: 3.828 ± 0.091
3.242AsnLys: 3.242 ± 0.071
4.566AsnLeu: 4.566 ± 0.091
0.997AsnMet: 0.997 ± 0.035
2.795AsnAsn: 2.795 ± 0.081
1.997AsnPro: 1.997 ± 0.057
2.924AsnGln: 2.924 ± 0.068
2.087AsnArg: 2.087 ± 0.057
3.091AsnSer: 3.091 ± 0.082
2.45AsnThr: 2.45 ± 0.066
2.318AsnVal: 2.318 ± 0.059
0.564AsnTrp: 0.564 ± 0.025
1.884AsnTyr: 1.884 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
2.742ProAla: 2.742 ± 0.066
0.405ProCys: 0.405 ± 0.022
1.786ProAsp: 1.786 ± 0.049
2.784ProGlu: 2.784 ± 0.064
1.599ProPhe: 1.599 ± 0.046
2.348ProGly: 2.348 ± 0.062
0.932ProHis: 0.932 ± 0.032
2.397ProIle: 2.397 ± 0.056
2.133ProLys: 2.133 ± 0.058
3.763ProLeu: 3.763 ± 0.076
0.938ProMet: 0.938 ± 0.036
1.558ProAsn: 1.558 ± 0.045
1.118ProPro: 1.118 ± 0.044
1.504ProGln: 1.504 ± 0.05
1.303ProArg: 1.303 ± 0.048
2.323ProSer: 2.323 ± 0.06
2.046ProThr: 2.046 ± 0.055
2.695ProVal: 2.695 ± 0.057
0.457ProTrp: 0.457 ± 0.027
1.488ProTyr: 1.488 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
4.857GlnAla: 4.857 ± 0.09
0.65GlnCys: 0.65 ± 0.031
2.059GlnAsp: 2.059 ± 0.049
2.987GlnGlu: 2.987 ± 0.066
1.861GlnPhe: 1.861 ± 0.047
3.44GlnGly: 3.44 ± 0.067
1.5GlnHis: 1.5 ± 0.053
2.629GlnIle: 2.629 ± 0.059
2.57GlnLys: 2.57 ± 0.054
5.715GlnLeu: 5.715 ± 0.091
1.007GlnMet: 1.007 ± 0.033
1.963GlnAsn: 1.963 ± 0.054
1.89GlnPro: 1.89 ± 0.046
3.49GlnGln: 3.49 ± 0.076
2.679GlnArg: 2.679 ± 0.075
3.198GlnSer: 3.198 ± 0.066
2.392GlnThr: 2.392 ± 0.056
3.403GlnVal: 3.403 ± 0.064
0.542GlnTrp: 0.542 ± 0.029
1.603GlnTyr: 1.603 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
3.377ArgAla: 3.377 ± 0.075
0.599ArgCys: 0.599 ± 0.025
2.184ArgAsp: 2.184 ± 0.056
2.674ArgGlu: 2.674 ± 0.073
2.293ArgPhe: 2.293 ± 0.05
2.549ArgGly: 2.549 ± 0.066
1.31ArgHis: 1.31 ± 0.042
2.777ArgIle: 2.777 ± 0.06
2.633ArgLys: 2.633 ± 0.065
5.386ArgLeu: 5.386 ± 0.094
1.082ArgMet: 1.082 ± 0.037
1.735ArgAsn: 1.735 ± 0.047
1.657ArgPro: 1.657 ± 0.05
2.416ArgGln: 2.416 ± 0.062
2.52ArgArg: 2.52 ± 0.068
2.665ArgSer: 2.665 ± 0.056
1.89ArgThr: 1.89 ± 0.044
3.002ArgVal: 3.002 ± 0.065
0.703ArgTrp: 0.703 ± 0.031
2.059ArgTyr: 2.059 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
5.4SerAla: 5.4 ± 0.09
0.789SerCys: 0.789 ± 0.033
3.3SerAsp: 3.3 ± 0.069
3.573SerGlu: 3.573 ± 0.067
3.063SerPhe: 3.063 ± 0.08
4.342SerGly: 4.342 ± 0.079
1.937SerHis: 1.937 ± 0.051
4.412SerIle: 4.412 ± 0.075
3.747SerLys: 3.747 ± 0.085
7.647SerLeu: 7.647 ± 0.103
1.696SerMet: 1.696 ± 0.049
2.81SerAsn: 2.81 ± 0.075
2.736SerPro: 2.736 ± 0.07
3.54SerGln: 3.54 ± 0.078
2.943SerArg: 2.943 ± 0.064
4.921SerSer: 4.921 ± 0.102
3.657SerThr: 3.657 ± 0.071
3.97SerVal: 3.97 ± 0.078
0.735SerTrp: 0.735 ± 0.03
2.469SerTyr: 2.469 ± 0.066
0.0SerXaa: 0.0 ± 0.0
Thr
4.745ThrAla: 4.745 ± 0.093
0.506ThrCys: 0.506 ± 0.024
2.632ThrAsp: 2.632 ± 0.054
2.924ThrGlu: 2.924 ± 0.068
1.826ThrPhe: 1.826 ± 0.054
3.787ThrGly: 3.787 ± 0.075
1.417ThrHis: 1.417 ± 0.043
3.52ThrIle: 3.52 ± 0.072
2.51ThrLys: 2.51 ± 0.067
6.298ThrLeu: 6.298 ± 0.102
1.131ThrMet: 1.131 ± 0.038
2.091ThrAsn: 2.091 ± 0.057
2.462ThrPro: 2.462 ± 0.065
2.443ThrGln: 2.443 ± 0.056
2.169ThrArg: 2.169 ± 0.049
3.328ThrSer: 3.328 ± 0.067
3.088ThrThr: 3.088 ± 0.077
3.8ThrVal: 3.8 ± 0.08
0.475ThrTrp: 0.475 ± 0.023
1.476ThrTyr: 1.476 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
5.352ValAla: 5.352 ± 0.086
0.828ValCys: 0.828 ± 0.039
3.397ValAsp: 3.397 ± 0.071
3.412ValGlu: 3.412 ± 0.077
2.465ValPhe: 2.465 ± 0.066
3.899ValGly: 3.899 ± 0.085
1.273ValHis: 1.273 ± 0.043
5.031ValIle: 5.031 ± 0.086
3.937ValLys: 3.937 ± 0.082
6.158ValLeu: 6.158 ± 0.106
1.679ValMet: 1.679 ± 0.056
2.95ValAsn: 2.95 ± 0.071
2.169ValPro: 2.169 ± 0.05
2.285ValGln: 2.285 ± 0.061
2.829ValArg: 2.829 ± 0.065
4.24ValSer: 4.24 ± 0.077
3.578ValThr: 3.578 ± 0.063
4.167ValVal: 4.167 ± 0.093
0.648ValTrp: 0.648 ± 0.027
1.913ValTyr: 1.913 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.674TrpAla: 0.674 ± 0.032
0.127TrpCys: 0.127 ± 0.013
0.427TrpAsp: 0.427 ± 0.032
0.355TrpGlu: 0.355 ± 0.02
0.501TrpPhe: 0.501 ± 0.025
0.626TrpGly: 0.626 ± 0.026
0.376TrpHis: 0.376 ± 0.024
0.543TrpIle: 0.543 ± 0.028
0.497TrpLys: 0.497 ± 0.023
1.612TrpLeu: 1.612 ± 0.048
0.261TrpMet: 0.261 ± 0.021
0.412TrpAsn: 0.412 ± 0.024
0.39TrpPro: 0.39 ± 0.02
0.987TrpGln: 0.987 ± 0.036
0.585TrpArg: 0.585 ± 0.024
0.704TrpSer: 0.704 ± 0.027
0.349TrpThr: 0.349 ± 0.022
0.711TrpVal: 0.711 ± 0.027
0.127TrpTrp: 0.127 ± 0.011
0.371TrpTyr: 0.371 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.538TyrAla: 2.538 ± 0.063
0.575TyrCys: 0.575 ± 0.031
1.625TyrAsp: 1.625 ± 0.043
1.593TyrGlu: 1.593 ± 0.054
1.73TyrPhe: 1.73 ± 0.054
2.226TyrGly: 2.226 ± 0.053
1.011TyrHis: 1.011 ± 0.042
2.325TyrIle: 2.325 ± 0.049
1.831TyrLys: 1.831 ± 0.045
4.138TyrLeu: 4.138 ± 0.085
0.606TyrMet: 0.606 ± 0.028
1.493TyrAsn: 1.493 ± 0.05
1.308TyrPro: 1.308 ± 0.042
2.527TyrGln: 2.527 ± 0.067
1.712TyrArg: 1.712 ± 0.044
2.484TyrSer: 2.484 ± 0.056
1.663TyrThr: 1.663 ± 0.047
1.625TyrVal: 1.625 ± 0.045
0.37TyrTrp: 0.37 ± 0.023
1.348TyrTyr: 1.348 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2947 proteins (831806 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski