Hubei sobemo-like virus 13

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KF10|A0A1L3KF10_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 13 OX=1923198 PE=4 SV=1
MM1 pKa = 7.35NSVPGVCSLSRR12 pKa = 11.84LGTTNAQVFGMDD24 pKa = 3.13AEE26 pKa = 4.64GRR28 pKa = 11.84FDD30 pKa = 4.51KK31 pKa = 11.21SRR33 pKa = 11.84LDD35 pKa = 3.65LVYY38 pKa = 9.8TAVKK42 pKa = 10.29DD43 pKa = 3.63RR44 pKa = 11.84YY45 pKa = 10.39LAVTQGKK52 pKa = 7.38TEE54 pKa = 4.16SDD56 pKa = 3.5PLKK59 pKa = 10.9VFVKK63 pKa = 9.96QEE65 pKa = 3.67PHH67 pKa = 6.33KK68 pKa = 11.0VEE70 pKa = 3.92KK71 pKa = 10.99LKK73 pKa = 10.77DD74 pKa = 3.37DD75 pKa = 3.89RR76 pKa = 11.84LRR78 pKa = 11.84LIMSVSLVDD87 pKa = 3.79ALVDD91 pKa = 4.61RR92 pKa = 11.84ILFMRR97 pKa = 11.84LANQVIKK104 pKa = 10.79KK105 pKa = 8.14HH106 pKa = 5.15TLTNILIGWSPLGGGYY122 pKa = 10.49RR123 pKa = 11.84LIDD126 pKa = 3.4ALFPGEE132 pKa = 4.62TISIDD137 pKa = 3.14KK138 pKa = 10.46KK139 pKa = 9.49AWDD142 pKa = 3.34WSVPYY147 pKa = 9.7WLLEE151 pKa = 3.89AVKK154 pKa = 10.61GVIKK158 pKa = 10.58RR159 pKa = 11.84LAVDD163 pKa = 4.45APQWWHH169 pKa = 6.83DD170 pKa = 3.65AVDD173 pKa = 3.68TRR175 pKa = 11.84FSCLFGDD182 pKa = 4.17PEE184 pKa = 4.51FVFPDD189 pKa = 3.87GTRR192 pKa = 11.84GRR194 pKa = 11.84QEE196 pKa = 3.48QPGIMKK202 pKa = 10.01SGCYY206 pKa = 8.45LTILINSIAQLVLHH220 pKa = 6.47EE221 pKa = 4.2MAKK224 pKa = 10.38VSLGLGDD231 pKa = 3.67EE232 pKa = 4.4VEE234 pKa = 4.36PVVVLGDD241 pKa = 4.35DD242 pKa = 3.46SLQRR246 pKa = 11.84KK247 pKa = 8.11FDD249 pKa = 3.19RR250 pKa = 11.84WQEE253 pKa = 3.82YY254 pKa = 9.75VDD256 pKa = 4.4YY257 pKa = 9.43ITALGFRR264 pKa = 11.84CEE266 pKa = 4.28VEE268 pKa = 4.03HH269 pKa = 7.56HH270 pKa = 6.35SDD272 pKa = 3.4EE273 pKa = 4.68PEE275 pKa = 3.9FAGFRR280 pKa = 11.84YY281 pKa = 9.52RR282 pKa = 11.84DD283 pKa = 3.61KK284 pKa = 11.09FKK286 pKa = 10.09PAYY289 pKa = 8.58FQKK292 pKa = 10.78HH293 pKa = 5.62LFNLAHH299 pKa = 6.69LTTDD303 pKa = 3.91RR304 pKa = 11.84EE305 pKa = 4.56VAQQTLQNYY314 pKa = 8.23QLLYY318 pKa = 11.1YY319 pKa = 9.92FDD321 pKa = 3.91EE322 pKa = 4.41PMRR325 pKa = 11.84RR326 pKa = 11.84VITDD330 pKa = 3.53MIRR333 pKa = 11.84ILNMPEE339 pKa = 3.36AAADD343 pKa = 3.92EE344 pKa = 4.29LRR346 pKa = 11.84LRR348 pKa = 11.84ALAMGG353 pKa = 4.45

Molecular weight: 40.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF19|A0A1L3KF19_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 13 OX=1923198 PE=4 SV=1
MM1 pKa = 7.27LVVLRR6 pKa = 11.84YY7 pKa = 9.61IYY9 pKa = 10.19QKK11 pKa = 11.44GMLKK15 pKa = 9.61VASMLQKK22 pKa = 10.41SSRR25 pKa = 11.84NSKK28 pKa = 9.99LVYY31 pKa = 10.32GSIEE35 pKa = 3.79RR36 pKa = 11.84KK37 pKa = 9.31IRR39 pKa = 11.84IGPSLGMVGAWEE51 pKa = 4.11RR52 pKa = 11.84LSLQLPIILLKK63 pKa = 7.6MTHH66 pKa = 5.62YY67 pKa = 10.84VFAVLRR73 pKa = 11.84AFWTWNLSGFVIWVVTYY90 pKa = 10.14LQLISLTEE98 pKa = 3.91SGLSYY103 pKa = 10.6RR104 pKa = 11.84SRR106 pKa = 11.84LPGFQSWLSLILRR119 pKa = 11.84LYY121 pKa = 8.53MWAVQEE127 pKa = 4.01WHH129 pKa = 6.78LVACLSHH136 pKa = 6.49TLLILTLFMRR146 pKa = 11.84DD147 pKa = 3.2PLNPVFQAHH156 pKa = 6.86LCIQVILYY164 pKa = 7.16MACMWVQALAIWPWILIGSLPLLFATRR191 pKa = 11.84VPRR194 pKa = 11.84SLFSRR199 pKa = 11.84SFLLDD204 pKa = 3.17TNVQEE209 pKa = 4.25RR210 pKa = 11.84TLNSMVLHH218 pKa = 6.16LVNLGSCIMANIAFLMTRR236 pKa = 11.84IYY238 pKa = 10.71QNTYY242 pKa = 8.27GMCWNMLLRR251 pKa = 11.84QLNIMMRR258 pKa = 11.84GSRR261 pKa = 11.84TTHH264 pKa = 6.09FMIDD268 pKa = 3.44QEE270 pKa = 5.86DD271 pKa = 4.53PDD273 pKa = 4.03TDD275 pKa = 3.15QDD277 pKa = 3.93LRR279 pKa = 11.84LMIQPVSEE287 pKa = 4.11

Molecular weight: 33.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1144

287

504

381.3

43.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.381 ± 0.606

1.311 ± 0.14

5.07 ± 1.244

5.157 ± 0.899

4.371 ± 0.212

6.381 ± 0.821

2.273 ± 0.067

6.731 ± 0.812

4.196 ± 0.765

10.664 ± 2.52

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.234 ± 1.024

2.972 ± 0.296

4.808 ± 0.63

4.633 ± 0.21

6.643 ± 0.068

6.906 ± 0.986

5.157 ± 0.343

7.168 ± 0.455

2.535 ± 0.423

3.409 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski