Pseudoalteromonas phage C7
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411CVU7|A0A411CVU7_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage C7 OX=2510494 PE=4 SV=1
MM1 pKa = 7.98 PLTITPALIQTIYY14 pKa = 10.93 ANSYY18 pKa = 11.52 DD19 pKa = 4.3 DD20 pKa = 4.04 ATLQALIDD28 pKa = 4.55 DD29 pKa = 4.52 YY30 pKa = 11.33 EE31 pKa = 5.07 SKK33 pKa = 10.51 IGEE36 pKa = 4.39 CLSASVGDD44 pKa = 3.9 AVGGLVLANYY54 pKa = 9.29 IAYY57 pKa = 8.56 TIYY60 pKa = 11.03 DD61 pKa = 3.4 PTLTVKK67 pKa = 10.33 SEE69 pKa = 3.87 KK70 pKa = 10.67 GSFGDD75 pKa = 3.96 SVTYY79 pKa = 10.47 QDD81 pKa = 3.36 NTMGADD87 pKa = 3.33 AYY89 pKa = 10.55 LQRR92 pKa = 11.84 AQKK95 pKa = 9.82 MDD97 pKa = 3.62 TNNCLLSLQNTSFGLMTLGQRR118 pKa = 11.84 SS119 pKa = 3.29
Molecular weight: 12.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.871
IPC2_protein 3.986
IPC_protein 3.935
Toseland 3.706
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.923
Rodwell 3.757
Grimsley 3.617
Solomon 3.923
Lehninger 3.884
Nozaki 4.075
DTASelect 4.342
Thurlkill 3.783
EMBOSS 3.923
Sillero 4.062
Patrickios 0.362
IPC_peptide 3.923
IPC2_peptide 4.024
IPC2.peptide.svr19 3.946
Protein with the highest isoelectric point:
>tr|A0A411CW28|A0A411CW28_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage C7 OX=2510494 PE=4 SV=1
MM1 pKa = 6.2 VTSIEE6 pKa = 4.03 RR7 pKa = 11.84 QFIIDD12 pKa = 3.86 NPHH15 pKa = 6.38 LKK17 pKa = 10.72 SNAIQKK23 pKa = 10.4 FIDD26 pKa = 3.47 VSITHH31 pKa = 7.02 INRR34 pKa = 11.84 IKK36 pKa = 10.68 QEE38 pKa = 3.71 AHH40 pKa = 6.47 GKK42 pKa = 9.5 IKK44 pKa = 10.06 WFHH47 pKa = 6.71 KK48 pKa = 9.41 GNKK51 pKa = 8.86 YY52 pKa = 10.23 PYY54 pKa = 9.93 LNKK57 pKa = 10.58 GEE59 pKa = 3.9 NGYY62 pKa = 10.25 RR63 pKa = 11.84 IRR65 pKa = 11.84 YY66 pKa = 8.24 AGNLILRR73 pKa = 11.84 TRR75 pKa = 11.84 NPNTAFYY82 pKa = 10.47 VIDD85 pKa = 3.51 IMISGIEE92 pKa = 3.75 QGYY95 pKa = 10.15 FDD97 pKa = 5.24 GDD99 pKa = 4.29 RR100 pKa = 11.84 IPKK103 pKa = 8.86 PKK105 pKa = 10.47 KK106 pKa = 8.02 FTRR109 pKa = 11.84 LSFEE113 pKa = 4.97 LPSLMQIARR122 pKa = 11.84 KK123 pKa = 9.77 SDD125 pKa = 3.51 FKK127 pKa = 10.66 EE128 pKa = 3.78 TARR131 pKa = 11.84 TYY133 pKa = 10.75 KK134 pKa = 10.7
Molecular weight: 15.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.16
IPC2_protein 9.531
IPC_protein 9.589
Toseland 10.218
ProMoST 9.897
Dawson 10.394
Bjellqvist 10.028
Wikipedia 10.54
Rodwell 10.906
Grimsley 10.452
Solomon 10.423
Lehninger 10.394
Nozaki 10.189
DTASelect 10.028
Thurlkill 10.248
EMBOSS 10.613
Sillero 10.292
Patrickios 10.599
IPC_peptide 10.423
IPC2_peptide 8.536
IPC2.peptide.svr19 8.586
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
12731
29
837
174.4
19.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.965 ± 0.522
1.304 ± 0.154
6.19 ± 0.236
6.747 ± 0.228
4.045 ± 0.211
6.527 ± 0.203
1.626 ± 0.199
6.488 ± 0.211
7.258 ± 0.37
7.666 ± 0.258
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.781 ± 0.172
5.71 ± 0.23
3.495 ± 0.232
3.574 ± 0.226
4.163 ± 0.185
6.575 ± 0.205
6.315 ± 0.409
6.441 ± 0.233
1.249 ± 0.153
3.88 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here