Amino acid dipepetide frequency for Pseudoalteromonas phage C7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.463AlaAla: 7.463 ± 1.351
0.55AlaCys: 0.55 ± 0.214
4.635AlaAsp: 4.635 ± 0.503
4.085AlaGlu: 4.085 ± 0.621
3.456AlaPhe: 3.456 ± 0.544
4.478AlaGly: 4.478 ± 0.774
1.335AlaHis: 1.335 ± 0.317
5.342AlaIle: 5.342 ± 0.665
6.049AlaLys: 6.049 ± 0.892
6.991AlaLeu: 6.991 ± 0.969
2.749AlaMet: 2.749 ± 0.412
5.027AlaAsn: 5.027 ± 0.475
2.828AlaPro: 2.828 ± 0.454
2.985AlaGln: 2.985 ± 0.478
3.142AlaArg: 3.142 ± 0.534
3.849AlaSer: 3.849 ± 0.533
5.499AlaThr: 5.499 ± 0.892
5.106AlaVal: 5.106 ± 0.551
1.335AlaTrp: 1.335 ± 0.281
2.749AlaTyr: 2.749 ± 0.461
0.0AlaXaa: 0.0 ± 0.0
Cys
0.707CysAla: 0.707 ± 0.256
0.157CysCys: 0.157 ± 0.109
0.864CysAsp: 0.864 ± 0.328
0.943CysGlu: 0.943 ± 0.279
0.393CysPhe: 0.393 ± 0.156
0.943CysGly: 0.943 ± 0.272
0.314CysHis: 0.314 ± 0.146
0.943CysIle: 0.943 ± 0.248
1.257CysLys: 1.257 ± 0.347
1.021CysLeu: 1.021 ± 0.457
0.393CysMet: 0.393 ± 0.167
0.786CysAsn: 0.786 ± 0.312
0.314CysPro: 0.314 ± 0.298
0.393CysGln: 0.393 ± 0.156
0.393CysArg: 0.393 ± 0.175
0.943CysSer: 0.943 ± 0.281
0.786CysThr: 0.786 ± 0.227
0.628CysVal: 0.628 ± 0.251
0.236CysTrp: 0.236 ± 0.137
0.628CysTyr: 0.628 ± 0.187
0.0CysXaa: 0.0 ± 0.0
Asp
4.556AspAla: 4.556 ± 0.617
1.65AspCys: 1.65 ± 0.37
3.221AspAsp: 3.221 ± 0.477
4.635AspGlu: 4.635 ± 0.672
3.221AspPhe: 3.221 ± 0.452
5.106AspGly: 5.106 ± 0.621
0.707AspHis: 0.707 ± 0.273
4.006AspIle: 4.006 ± 0.529
4.399AspLys: 4.399 ± 0.578
4.556AspLeu: 4.556 ± 0.555
1.571AspMet: 1.571 ± 0.35
2.828AspAsn: 2.828 ± 0.471
1.65AspPro: 1.65 ± 0.346
1.178AspGln: 1.178 ± 0.252
2.514AspArg: 2.514 ± 0.354
4.949AspSer: 4.949 ± 0.505
4.321AspThr: 4.321 ± 0.588
3.692AspVal: 3.692 ± 0.56
0.393AspTrp: 0.393 ± 0.148
2.749AspTyr: 2.749 ± 0.396
0.0AspXaa: 0.0 ± 0.0
Glu
4.949GluAla: 4.949 ± 0.887
0.628GluCys: 0.628 ± 0.273
2.514GluAsp: 2.514 ± 0.576
2.514GluGlu: 2.514 ± 0.516
3.771GluPhe: 3.771 ± 0.49
3.142GluGly: 3.142 ± 0.49
1.178GluHis: 1.178 ± 0.297
4.87GluIle: 4.87 ± 0.608
6.049GluLys: 6.049 ± 0.633
6.756GluLeu: 6.756 ± 0.756
2.2GluMet: 2.2 ± 0.347
2.749GluAsn: 2.749 ± 0.401
2.514GluPro: 2.514 ± 0.418
3.456GluGln: 3.456 ± 0.536
3.614GluArg: 3.614 ± 0.499
4.949GluSer: 4.949 ± 0.556
2.592GluThr: 2.592 ± 0.509
3.692GluVal: 3.692 ± 0.561
1.335GluTrp: 1.335 ± 0.32
4.006GluTyr: 4.006 ± 0.502
0.0GluXaa: 0.0 ± 0.0
Phe
3.221PheAla: 3.221 ± 0.53
0.471PheCys: 0.471 ± 0.185
3.064PheAsp: 3.064 ± 0.451
2.907PheGlu: 2.907 ± 0.467
1.257PhePhe: 1.257 ± 0.334
3.299PheGly: 3.299 ± 0.502
0.314PheHis: 0.314 ± 0.153
3.535PheIle: 3.535 ± 0.462
2.828PheLys: 2.828 ± 0.631
1.807PheLeu: 1.807 ± 0.319
0.707PheMet: 0.707 ± 0.23
3.849PheAsn: 3.849 ± 0.581
1.1PhePro: 1.1 ± 0.315
0.55PheGln: 0.55 ± 0.216
1.493PheArg: 1.493 ± 0.369
2.907PheSer: 2.907 ± 0.523
3.064PheThr: 3.064 ± 0.727
2.042PheVal: 2.042 ± 0.524
0.55PheTrp: 0.55 ± 0.197
1.493PheTyr: 1.493 ± 0.272
0.0PheXaa: 0.0 ± 0.0
Gly
5.263GlyAla: 5.263 ± 0.666
0.786GlyCys: 0.786 ± 0.252
4.085GlyAsp: 4.085 ± 0.509
5.342GlyGlu: 5.342 ± 0.545
3.064GlyPhe: 3.064 ± 0.535
5.499GlyGly: 5.499 ± 0.6
0.943GlyHis: 0.943 ± 0.277
3.771GlyIle: 3.771 ± 0.509
5.734GlyLys: 5.734 ± 0.711
3.614GlyLeu: 3.614 ± 0.51
1.65GlyMet: 1.65 ± 0.358
4.163GlyAsn: 4.163 ± 0.581
0.864GlyPro: 0.864 ± 0.239
1.493GlyGln: 1.493 ± 0.28
2.985GlyArg: 2.985 ± 0.539
4.556GlySer: 4.556 ± 0.707
3.535GlyThr: 3.535 ± 0.721
5.106GlyVal: 5.106 ± 0.643
0.628GlyTrp: 0.628 ± 0.291
2.121GlyTyr: 2.121 ± 0.404
0.0GlyXaa: 0.0 ± 0.0
His
1.1HisAla: 1.1 ± 0.267
0.786HisCys: 0.786 ± 0.186
0.864HisAsp: 0.864 ± 0.279
0.864HisGlu: 0.864 ± 0.274
0.786HisPhe: 0.786 ± 0.263
1.021HisGly: 1.021 ± 0.305
0.236HisHis: 0.236 ± 0.132
1.335HisIle: 1.335 ± 0.403
0.943HisLys: 0.943 ± 0.308
1.335HisLeu: 1.335 ± 0.353
0.157HisMet: 0.157 ± 0.11
0.864HisAsn: 0.864 ± 0.234
1.021HisPro: 1.021 ± 0.269
0.393HisGln: 0.393 ± 0.186
0.471HisArg: 0.471 ± 0.191
1.021HisSer: 1.021 ± 0.293
1.1HisThr: 1.1 ± 0.274
1.1HisVal: 1.1 ± 0.349
0.079HisTrp: 0.079 ± 0.076
0.786HisTyr: 0.786 ± 0.244
0.0HisXaa: 0.0 ± 0.0
Ile
5.185IleAla: 5.185 ± 0.76
1.1IleCys: 1.1 ± 0.259
5.813IleAsp: 5.813 ± 0.699
5.656IleGlu: 5.656 ± 0.641
1.964IlePhe: 1.964 ± 0.376
3.535IleGly: 3.535 ± 0.592
0.786IleHis: 0.786 ± 0.301
3.849IleIle: 3.849 ± 0.651
5.263IleLys: 5.263 ± 0.609
3.221IleLeu: 3.221 ± 0.493
1.807IleMet: 1.807 ± 0.379
3.614IleAsn: 3.614 ± 0.469
2.828IlePro: 2.828 ± 0.497
1.807IleGln: 1.807 ± 0.347
2.042IleArg: 2.042 ± 0.415
4.478IleSer: 4.478 ± 0.554
4.949IleThr: 4.949 ± 0.623
3.614IleVal: 3.614 ± 0.455
0.707IleTrp: 0.707 ± 0.28
2.671IleTyr: 2.671 ± 0.455
0.0IleXaa: 0.0 ± 0.0
Lys
5.97LysAla: 5.97 ± 0.739
0.943LysCys: 0.943 ± 0.351
4.085LysAsp: 4.085 ± 0.479
4.478LysGlu: 4.478 ± 0.66
2.435LysPhe: 2.435 ± 0.453
4.556LysGly: 4.556 ± 0.567
1.964LysHis: 1.964 ± 0.458
4.635LysIle: 4.635 ± 0.514
4.949LysLys: 4.949 ± 0.672
6.363LysLeu: 6.363 ± 0.72
2.121LysMet: 2.121 ± 0.346
3.378LysAsn: 3.378 ± 0.488
3.928LysPro: 3.928 ± 0.686
3.456LysGln: 3.456 ± 0.451
2.907LysArg: 2.907 ± 0.454
4.556LysSer: 4.556 ± 0.601
3.299LysThr: 3.299 ± 0.478
4.792LysVal: 4.792 ± 0.566
1.178LysTrp: 1.178 ± 0.276
2.592LysTyr: 2.592 ± 0.525
0.0LysXaa: 0.0 ± 0.0
Leu
6.049LeuAla: 6.049 ± 0.72
1.257LeuCys: 1.257 ± 0.402
4.478LeuAsp: 4.478 ± 0.596
4.713LeuGlu: 4.713 ± 0.648
2.278LeuPhe: 2.278 ± 0.456
4.635LeuGly: 4.635 ± 0.422
1.65LeuHis: 1.65 ± 0.311
5.656LeuIle: 5.656 ± 0.739
5.734LeuLys: 5.734 ± 0.634
6.206LeuLeu: 6.206 ± 0.697
3.064LeuMet: 3.064 ± 0.497
4.321LeuAsn: 4.321 ± 0.567
1.885LeuPro: 1.885 ± 0.283
2.435LeuGln: 2.435 ± 0.409
3.535LeuArg: 3.535 ± 0.619
5.185LeuSer: 5.185 ± 0.47
5.342LeuThr: 5.342 ± 0.507
4.87LeuVal: 4.87 ± 0.71
0.707LeuTrp: 0.707 ± 0.226
2.671LeuTyr: 2.671 ± 0.435
0.0LeuXaa: 0.0 ± 0.0
Met
3.378MetAla: 3.378 ± 0.598
0.157MetCys: 0.157 ± 0.105
1.178MetAsp: 1.178 ± 0.277
1.335MetGlu: 1.335 ± 0.365
1.178MetPhe: 1.178 ± 0.305
1.807MetGly: 1.807 ± 0.371
0.707MetHis: 0.707 ± 0.256
1.414MetIle: 1.414 ± 0.303
2.121MetLys: 2.121 ± 0.432
1.964MetLeu: 1.964 ± 0.321
0.707MetMet: 0.707 ± 0.213
1.571MetAsn: 1.571 ± 0.434
1.493MetPro: 1.493 ± 0.33
0.628MetGln: 0.628 ± 0.214
1.257MetArg: 1.257 ± 0.351
2.2MetSer: 2.2 ± 0.496
2.749MetThr: 2.749 ± 0.468
1.178MetVal: 1.178 ± 0.251
0.314MetTrp: 0.314 ± 0.154
0.471MetTyr: 0.471 ± 0.163
0.0MetXaa: 0.0 ± 0.0
Asn
4.478AsnAla: 4.478 ± 0.525
0.707AsnCys: 0.707 ± 0.243
3.299AsnAsp: 3.299 ± 0.491
4.635AsnGlu: 4.635 ± 0.615
1.885AsnPhe: 1.885 ± 0.385
4.635AsnGly: 4.635 ± 0.606
0.786AsnHis: 0.786 ± 0.225
3.692AsnIle: 3.692 ± 0.437
4.635AsnLys: 4.635 ± 0.576
3.535AsnLeu: 3.535 ± 0.51
1.1AsnMet: 1.1 ± 0.293
2.749AsnAsn: 2.749 ± 0.514
2.592AsnPro: 2.592 ± 0.424
2.278AsnGln: 2.278 ± 0.377
2.749AsnArg: 2.749 ± 0.428
3.142AsnSer: 3.142 ± 0.446
2.985AsnThr: 2.985 ± 0.446
3.771AsnVal: 3.771 ± 0.553
0.55AsnTrp: 0.55 ± 0.192
2.907AsnTyr: 2.907 ± 0.435
0.0AsnXaa: 0.0 ± 0.0
Pro
2.749ProAla: 2.749 ± 0.583
0.079ProCys: 0.079 ± 0.073
2.278ProAsp: 2.278 ± 0.546
2.828ProGlu: 2.828 ± 0.518
1.021ProPhe: 1.021 ± 0.286
0.864ProGly: 0.864 ± 0.234
0.55ProHis: 0.55 ± 0.207
1.807ProIle: 1.807 ± 0.411
2.671ProLys: 2.671 ± 0.468
2.749ProLeu: 2.749 ± 0.524
1.021ProMet: 1.021 ± 0.313
2.278ProAsn: 2.278 ± 0.443
1.178ProPro: 1.178 ± 0.292
1.65ProGln: 1.65 ± 0.331
1.021ProArg: 1.021 ± 0.244
2.907ProSer: 2.907 ± 0.442
2.828ProThr: 2.828 ± 0.49
3.064ProVal: 3.064 ± 0.479
0.393ProTrp: 0.393 ± 0.168
1.021ProTyr: 1.021 ± 0.279
0.0ProXaa: 0.0 ± 0.0
Gln
3.142GlnAla: 3.142 ± 0.512
0.157GlnCys: 0.157 ± 0.097
2.278GlnAsp: 2.278 ± 0.403
2.514GlnGlu: 2.514 ± 0.428
0.786GlnPhe: 0.786 ± 0.208
1.335GlnGly: 1.335 ± 0.329
0.55GlnHis: 0.55 ± 0.267
2.749GlnIle: 2.749 ± 0.528
2.671GlnLys: 2.671 ± 0.436
3.064GlnLeu: 3.064 ± 0.466
1.414GlnMet: 1.414 ± 0.299
1.885GlnAsn: 1.885 ± 0.412
1.414GlnPro: 1.414 ± 0.43
2.514GlnGln: 2.514 ± 0.469
1.493GlnArg: 1.493 ± 0.271
2.671GlnSer: 2.671 ± 0.502
1.885GlnThr: 1.885 ± 0.493
1.885GlnVal: 1.885 ± 0.522
0.471GlnTrp: 0.471 ± 0.186
0.864GlnTyr: 0.864 ± 0.22
0.0GlnXaa: 0.0 ± 0.0
Arg
3.064ArgAla: 3.064 ± 0.398
0.314ArgCys: 0.314 ± 0.148
3.064ArgAsp: 3.064 ± 0.416
2.592ArgGlu: 2.592 ± 0.411
2.121ArgPhe: 2.121 ± 0.451
2.357ArgGly: 2.357 ± 0.427
1.1ArgHis: 1.1 ± 0.436
2.671ArgIle: 2.671 ± 0.438
3.064ArgLys: 3.064 ± 0.334
3.456ArgLeu: 3.456 ± 0.466
0.786ArgMet: 0.786 ± 0.203
1.885ArgAsn: 1.885 ± 0.301
1.021ArgPro: 1.021 ± 0.305
1.021ArgGln: 1.021 ± 0.242
2.121ArgArg: 2.121 ± 0.426
2.357ArgSer: 2.357 ± 0.482
2.749ArgThr: 2.749 ± 0.595
3.142ArgVal: 3.142 ± 0.507
0.707ArgTrp: 0.707 ± 0.193
2.042ArgTyr: 2.042 ± 0.418
0.0ArgXaa: 0.0 ± 0.0
Ser
4.163SerAla: 4.163 ± 0.622
0.943SerCys: 0.943 ± 0.353
4.321SerAsp: 4.321 ± 0.576
4.556SerGlu: 4.556 ± 0.611
3.221SerPhe: 3.221 ± 0.485
5.97SerGly: 5.97 ± 0.609
0.864SerHis: 0.864 ± 0.252
4.556SerIle: 4.556 ± 0.57
3.614SerLys: 3.614 ± 0.548
5.499SerLeu: 5.499 ± 0.607
2.2SerMet: 2.2 ± 0.414
4.085SerAsn: 4.085 ± 0.425
2.121SerPro: 2.121 ± 0.395
2.121SerGln: 2.121 ± 0.398
2.278SerArg: 2.278 ± 0.482
3.849SerSer: 3.849 ± 0.504
3.614SerThr: 3.614 ± 0.524
4.87SerVal: 4.87 ± 0.729
0.707SerTrp: 0.707 ± 0.244
2.2SerTyr: 2.2 ± 0.39
0.0SerXaa: 0.0 ± 0.0
Thr
5.892ThrAla: 5.892 ± 0.902
0.471ThrCys: 0.471 ± 0.217
3.299ThrAsp: 3.299 ± 0.489
4.635ThrGlu: 4.635 ± 0.577
2.357ThrPhe: 2.357 ± 0.468
4.085ThrGly: 4.085 ± 0.687
0.943ThrHis: 0.943 ± 0.257
4.006ThrIle: 4.006 ± 0.674
3.378ThrLys: 3.378 ± 0.529
4.399ThrLeu: 4.399 ± 0.722
1.178ThrMet: 1.178 ± 0.323
4.006ThrAsn: 4.006 ± 0.567
2.671ThrPro: 2.671 ± 0.473
3.064ThrGln: 3.064 ± 0.482
2.2ThrArg: 2.2 ± 0.457
3.456ThrSer: 3.456 ± 0.468
4.635ThrThr: 4.635 ± 0.889
5.185ThrVal: 5.185 ± 0.636
0.628ThrTrp: 0.628 ± 0.226
2.671ThrTyr: 2.671 ± 0.52
0.0ThrXaa: 0.0 ± 0.0
Val
4.478ValAla: 4.478 ± 0.586
0.943ValCys: 0.943 ± 0.25
4.635ValAsp: 4.635 ± 0.6
4.713ValGlu: 4.713 ± 0.537
2.514ValPhe: 2.514 ± 0.36
4.635ValGly: 4.635 ± 0.53
0.471ValHis: 0.471 ± 0.183
3.378ValIle: 3.378 ± 0.695
3.771ValLys: 3.771 ± 0.422
5.027ValLeu: 5.027 ± 0.604
1.728ValMet: 1.728 ± 0.36
5.027ValAsn: 5.027 ± 0.699
1.65ValPro: 1.65 ± 0.33
2.278ValGln: 2.278 ± 0.532
2.435ValArg: 2.435 ± 0.335
4.399ValSer: 4.399 ± 0.61
4.163ValThr: 4.163 ± 0.693
4.635ValVal: 4.635 ± 0.626
1.1ValTrp: 1.1 ± 0.289
2.435ValTyr: 2.435 ± 0.419
0.0ValXaa: 0.0 ± 0.0
Trp
0.864TrpAla: 0.864 ± 0.232
0.471TrpCys: 0.471 ± 0.196
0.707TrpAsp: 0.707 ± 0.211
0.786TrpGlu: 0.786 ± 0.224
0.786TrpPhe: 0.786 ± 0.215
0.314TrpGly: 0.314 ± 0.149
0.236TrpHis: 0.236 ± 0.12
0.471TrpIle: 0.471 ± 0.2
0.707TrpLys: 0.707 ± 0.293
1.65TrpLeu: 1.65 ± 0.356
0.314TrpMet: 0.314 ± 0.154
0.393TrpAsn: 0.393 ± 0.203
0.471TrpPro: 0.471 ± 0.191
0.943TrpGln: 0.943 ± 0.253
1.021TrpArg: 1.021 ± 0.251
0.471TrpSer: 0.471 ± 0.184
0.157TrpThr: 0.157 ± 0.102
0.864TrpVal: 0.864 ± 0.282
0.236TrpTrp: 0.236 ± 0.129
0.628TrpTyr: 0.628 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.985TyrAla: 2.985 ± 0.465
0.471TyrCys: 0.471 ± 0.176
3.221TyrAsp: 3.221 ± 0.407
2.749TyrGlu: 2.749 ± 0.492
1.964TyrPhe: 1.964 ± 0.334
3.064TyrGly: 3.064 ± 0.577
0.628TyrHis: 0.628 ± 0.244
2.2TyrIle: 2.2 ± 0.38
2.749TyrLys: 2.749 ± 0.44
3.456TyrLeu: 3.456 ± 0.633
0.864TyrMet: 0.864 ± 0.293
1.65TyrAsn: 1.65 ± 0.314
1.414TyrPro: 1.414 ± 0.328
1.1TyrGln: 1.1 ± 0.315
1.964TyrArg: 1.964 ± 0.326
2.907TyrSer: 2.907 ± 0.459
2.907TyrThr: 2.907 ± 0.538
1.178TyrVal: 1.178 ± 0.363
0.236TyrTrp: 0.236 ± 0.122
1.1TyrTyr: 1.1 ± 0.335
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (12731 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski