Beet curly top virus (strain California/Logan) (BCTV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Curtovirus; Beet curly top virus

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q91J24|AC4_BCTVC Protein C4 OS=Beet curly top virus (strain California/Logan) OX=268960 GN=C4 PE=1 SV=2
MM1 pKa = 7.49GPFRR5 pKa = 11.84VDD7 pKa = 3.04QFPDD11 pKa = 3.13NYY13 pKa = 10.04PAFLAVSTSCFLRR26 pKa = 11.84YY27 pKa = 9.62NRR29 pKa = 11.84WCILGIHH36 pKa = 5.43QEE38 pKa = 4.06IGSLTLEE45 pKa = 4.12EE46 pKa = 4.92GEE48 pKa = 5.07VFRR51 pKa = 11.84QFQKK55 pKa = 10.6EE56 pKa = 4.32VKK58 pKa = 9.94KK59 pKa = 10.84LLRR62 pKa = 11.84CKK64 pKa = 10.85VNFHH68 pKa = 6.79RR69 pKa = 11.84KK70 pKa = 8.25CSLYY74 pKa = 10.36EE75 pKa = 4.06EE76 pKa = 4.87IYY78 pKa = 10.45KK79 pKa = 10.49KK80 pKa = 10.64YY81 pKa = 10.63VYY83 pKa = 10.35NVPEE87 pKa = 4.25KK88 pKa = 10.48KK89 pKa = 10.52GEE91 pKa = 4.03SSKK94 pKa = 11.04CVAEE98 pKa = 4.25EE99 pKa = 4.37EE100 pKa = 4.19EE101 pKa = 4.5DD102 pKa = 3.5

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P14991|REP_BCTVC Replication-associated protein OS=Beet curly top virus (strain California/Logan) OX=268960 GN=C1 PE=3 SV=2
MM1 pKa = 7.88RR2 pKa = 11.84KK3 pKa = 7.89YY4 pKa = 9.06TRR6 pKa = 11.84NTYY9 pKa = 9.32TMSQKK14 pKa = 10.61RR15 pKa = 11.84KK16 pKa = 10.04VNPQSAWPKK25 pKa = 9.85KK26 pKa = 9.73RR27 pKa = 11.84RR28 pKa = 11.84TSTTSRR34 pKa = 11.84KK35 pKa = 7.86YY36 pKa = 7.06QWRR39 pKa = 11.84RR40 pKa = 11.84PVTKK44 pKa = 10.6NRR46 pKa = 11.84TLKK49 pKa = 10.94LKK51 pKa = 10.1MYY53 pKa = 10.7DD54 pKa = 3.77DD55 pKa = 4.06MLGAGGIGSTISNNGMITMLNNYY78 pKa = 7.83VQGIGDD84 pKa = 3.7SQRR87 pKa = 11.84ARR89 pKa = 11.84NVTVTKK95 pKa = 10.4HH96 pKa = 6.17LKK98 pKa = 10.2FDD100 pKa = 3.62MALMGSSQFWEE111 pKa = 4.58TPNYY115 pKa = 5.21MTQYY119 pKa = 10.02HH120 pKa = 7.06WIIIDD125 pKa = 3.58KK126 pKa = 10.67DD127 pKa = 3.75VGSVFPTKK135 pKa = 10.48LSSIFDD141 pKa = 3.63IPDD144 pKa = 3.36NGQAMPSTYY153 pKa = 10.14RR154 pKa = 11.84IRR156 pKa = 11.84RR157 pKa = 11.84DD158 pKa = 3.1MNEE161 pKa = 3.47RR162 pKa = 11.84FIVKK166 pKa = 9.94KK167 pKa = 10.01KK168 pKa = 9.3WRR170 pKa = 11.84THH172 pKa = 5.48LMSTGTGYY180 pKa = 10.86GGKK183 pKa = 8.13EE184 pKa = 4.05TYY186 pKa = 9.63KK187 pKa = 10.75APSMPNYY194 pKa = 9.03KK195 pKa = 10.18KK196 pKa = 10.51PMNINVRR203 pKa = 11.84NLNMRR208 pKa = 11.84TIWKK212 pKa = 8.04DD213 pKa = 3.07TGGGKK218 pKa = 10.11YY219 pKa = 10.33EE220 pKa = 4.09DD221 pKa = 3.65VKK223 pKa = 11.35EE224 pKa = 3.97NALLYY229 pKa = 10.77VVVNDD234 pKa = 3.71NTDD237 pKa = 3.47NTNMYY242 pKa = 8.08ATLFGNCRR250 pKa = 11.84CYY252 pKa = 10.31FYY254 pKa = 11.55

Molecular weight: 29.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1196

85

358

170.9

19.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.93 ± 0.919

1.589 ± 0.418

4.097 ± 0.577

6.02 ± 1.131

4.097 ± 0.498

5.518 ± 0.432

2.508 ± 0.506

6.02 ± 0.569

6.355 ± 0.872

8.528 ± 1.17

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.908

6.522 ± 0.931

5.1 ± 0.44

5.268 ± 0.738

6.355 ± 0.774

7.86 ± 1.08

6.522 ± 1.079

5.017 ± 0.566

1.589 ± 0.253

4.515 ± 0.485
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski