Veillonellaceae bacterium DNF00626
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1608 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A134CHU1|A0A134CHU1_9FIRM Uncharacterized protein OS=Veillonellaceae bacterium DNF00626 OX=1588754 GN=HMPREF3191_00914 PE=4 SV=1
MM1 pKa = 6.98 QEE3 pKa = 3.68 KK4 pKa = 10.63 GIDD7 pKa = 3.45 VSEE10 pKa = 4.3 NNGVIDD16 pKa = 3.26 WDD18 pKa = 4.15 EE19 pKa = 4.22 IKK21 pKa = 11.16 NEE23 pKa = 4.17 GYY25 pKa = 9.67 EE26 pKa = 3.98 FAIIRR31 pKa = 11.84 CGYY34 pKa = 9.12 GKK36 pKa = 10.55 SSVDD40 pKa = 3.14 SMFYY44 pKa = 11.25 DD45 pKa = 3.72 NVNGAQDD52 pKa = 3.66 AGLKK56 pKa = 9.9 IGVYY60 pKa = 9.37 HH61 pKa = 6.34 YY62 pKa = 11.01 SYY64 pKa = 11.68 ALTVEE69 pKa = 4.44 DD70 pKa = 4.48 AMEE73 pKa = 3.97 EE74 pKa = 4.39 AEE76 pKa = 5.4 FVTALLEE83 pKa = 4.61 DD84 pKa = 4.85 CGLDD88 pKa = 3.6 DD89 pKa = 4.98 QSKK92 pKa = 11.35 LEE94 pKa = 4.02 MKK96 pKa = 9.98 VWFDD100 pKa = 3.74 MEE102 pKa = 5.9 DD103 pKa = 3.32 SDD105 pKa = 5.65 GYY107 pKa = 10.01 KK108 pKa = 10.14 ARR110 pKa = 11.84 HH111 pKa = 5.8 GVTDD115 pKa = 3.66 SATLTDD121 pKa = 3.3 ICNVFLNTLYY131 pKa = 11.02 DD132 pKa = 3.0 RR133 pKa = 11.84 GYY135 pKa = 11.31 DD136 pKa = 3.29 VGLYY140 pKa = 10.7 CNLDD144 pKa = 3.41 YY145 pKa = 11.6 AEE147 pKa = 4.54 NVLYY151 pKa = 8.04 MTEE154 pKa = 4.11 LKK156 pKa = 10.52 EE157 pKa = 4.0 FPIWIAQYY165 pKa = 11.43 NSTCDD170 pKa = 3.7 FPSAFMWQYY179 pKa = 10.96 TDD181 pKa = 4.73 SEE183 pKa = 4.98 DD184 pKa = 4.39 INGKK188 pKa = 9.72 CFDD191 pKa = 4.7 GDD193 pKa = 3.57 VLMYY197 pKa = 10.94
Molecular weight: 22.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.828
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.605
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.516
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.151
Thurlkill 3.656
EMBOSS 3.745
Sillero 3.935
Patrickios 0.273
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A134CMK9|A0A134CMK9_9FIRM Putative ferrichrome ABC transporter ATP-binding protein FhuC OS=Veillonellaceae bacterium DNF00626 OX=1588754 GN=HMPREF3191_00246 PE=4 SV=1
MM1 pKa = 7.7 AKK3 pKa = 7.99 MTFQPNNHH11 pKa = 5.38 WRR13 pKa = 11.84 KK14 pKa = 7.63 KK15 pKa = 6.32 THH17 pKa = 5.67 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MKK24 pKa = 8.69 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 IVLKK33 pKa = 10.39 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.89 GRR40 pKa = 11.84 KK41 pKa = 8.67 VLSAA45 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.676
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.398
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1608
0
1608
480156
34
4599
298.6
33.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.64 ± 0.076
1.097 ± 0.027
5.589 ± 0.052
6.947 ± 0.07
3.966 ± 0.056
7.65 ± 0.086
2.16 ± 0.032
7.794 ± 0.064
7.551 ± 0.069
8.633 ± 0.084
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.076 ± 0.035
4.418 ± 0.072
3.368 ± 0.044
3.026 ± 0.034
4.329 ± 0.05
5.582 ± 0.043
5.594 ± 0.06
6.948 ± 0.049
0.974 ± 0.017
3.659 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here