Arsenophonus sp. ENCA
Average proteome isoelectric point is 7.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3133 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A2HJZ7|A0A2A2HJZ7_9GAMM Peptidyl-prolyl cis-trans isomerase OS=Arsenophonus sp. ENCA OX=1987579 GN=CBG25_02745 PE=3 SV=1
MM1 pKa = 7.81 KK2 pKa = 10.19 IGLFYY7 pKa = 10.96 GSSTCYY13 pKa = 10.32 TEE15 pKa = 4.37 MVAEE19 pKa = 5.03 KK20 pKa = 10.17 IQYY23 pKa = 10.03 NLGKK27 pKa = 10.3 EE28 pKa = 4.15 LVDD31 pKa = 4.96 LYY33 pKa = 10.91 NLKK36 pKa = 10.64 DD37 pKa = 3.43 ISPQLMEE44 pKa = 4.84 NYY46 pKa = 9.89 QILILGIPTWDD57 pKa = 3.34 FGEE60 pKa = 4.75 LQEE63 pKa = 4.66 DD64 pKa = 3.83 WQAIWDD70 pKa = 4.11 EE71 pKa = 4.36 LCSLNLAGKK80 pKa = 9.53 IVSLYY85 pKa = 11.17 GLGDD89 pKa = 3.72 QIDD92 pKa = 3.93 YY93 pKa = 11.36 SDD95 pKa = 3.9 WFLDD99 pKa = 3.65 ALGMLYY105 pKa = 10.22 EE106 pKa = 4.12 QLLRR110 pKa = 11.84 MQVKK114 pKa = 9.12 FVGFWPTTGYY124 pKa = 8.74 TFSSLKK130 pKa = 10.38 PLTADD135 pKa = 3.79 GSQFVGLALDD145 pKa = 3.57 EE146 pKa = 4.55 TNQFEE151 pKa = 4.5 QTDD154 pKa = 3.61 EE155 pKa = 6.02 RR156 pKa = 11.84 IEE158 pKa = 3.97 QWCMQILNEE167 pKa = 4.84 IEE169 pKa = 4.09 ALLL172 pKa = 4.07
Molecular weight: 19.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 3.923
IPC_protein 3.872
Toseland 3.668
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.592
Solomon 3.834
Lehninger 3.783
Nozaki 3.961
DTASelect 4.126
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.986
Patrickios 0.693
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.883
Protein with the highest isoelectric point:
>tr|A0A2A2GZU8|A0A2A2GZU8_9GAMM Uncharacterized protein OS=Arsenophonus sp. ENCA OX=1987579 GN=CBG25_10255 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSSKK47 pKa = 11.16
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3133
0
3133
722245
25
1715
230.5
25.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.003 ± 0.048
1.133 ± 0.018
5.011 ± 0.036
5.858 ± 0.05
4.019 ± 0.035
6.218 ± 0.039
2.287 ± 0.023
7.561 ± 0.052
6.326 ± 0.049
10.389 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.558 ± 0.023
4.883 ± 0.035
3.872 ± 0.027
4.77 ± 0.041
5.193 ± 0.042
6.081 ± 0.039
5.448 ± 0.032
5.945 ± 0.037
1.257 ± 0.019
3.169 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here