Amino acid dipepetide frequency for Arsenophonus sp. ENCA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.855AlaAla: 6.855 ± 0.125	1.001AlaCys: 1.001 ± 0.039	4.215AlaAsp: 4.215 ± 0.077	5.357AlaGlu: 5.357 ± 0.107	2.948AlaPhe: 2.948 ± 0.063	5.295AlaGly: 5.295 ± 0.092	1.544AlaHis: 1.544 ± 0.047	6.452AlaIle: 6.452 ± 0.107	5.259AlaLys: 5.259 ± 0.081	8.785AlaLeu: 8.785 ± 0.138	2.345AlaMet: 2.345 ± 0.058	3.718AlaAsn: 3.718 ± 0.082	2.362AlaPro: 2.362 ± 0.059	3.442AlaGln: 3.442 ± 0.071	3.982AlaArg: 3.982 ± 0.084	4.253AlaSer: 4.253 ± 0.092	4.168AlaThr: 4.168 ± 0.077	4.78AlaVal: 4.78 ± 0.09	0.973AlaTrp: 0.973 ± 0.039	2.286AlaTyr: 2.286 ± 0.056	0.011AlaXaa: 0.011 ± 0.005
Cys	0.735CysAla: 0.735 ± 0.033	0.251CysCys: 0.251 ± 0.017	0.662CysAsp: 0.662 ± 0.029	0.565CysGlu: 0.565 ± 0.029	0.561CysPhe: 0.561 ± 0.028	0.933CysGly: 0.933 ± 0.036	0.345CysHis: 0.345 ± 0.022	0.708CysIle: 0.708 ± 0.033	0.494CysLys: 0.494 ± 0.024	1.12CysLeu: 1.12 ± 0.038	0.277CysMet: 0.277 ± 0.018	0.374CysAsn: 0.374 ± 0.022	0.469CysPro: 0.469 ± 0.029	0.666CysGln: 0.666 ± 0.033	0.672CysArg: 0.672 ± 0.032	0.67CysSer: 0.67 ± 0.028	0.44CysThr: 0.44 ± 0.021	0.691CysVal: 0.691 ± 0.032	0.198CysTrp: 0.198 ± 0.017	0.496CysTyr: 0.496 ± 0.028	0.001CysXaa: 0.001 ± 0.001
Asp	3.64AspAla: 3.64 ± 0.074	0.611AspCys: 0.611 ± 0.026	2.477AspAsp: 2.477 ± 0.064	3.571AspGlu: 3.571 ± 0.065	2.33AspPhe: 2.33 ± 0.065	3.108AspGly: 3.108 ± 0.072	0.89AspHis: 0.89 ± 0.036	4.242AspIle: 4.242 ± 0.078	3.608AspLys: 3.608 ± 0.079	4.515AspLeu: 4.515 ± 0.09	1.405AspMet: 1.405 ± 0.044	2.753AspAsn: 2.753 ± 0.07	1.902AspPro: 1.902 ± 0.056	1.535AspGln: 1.535 ± 0.046	2.249AspArg: 2.249 ± 0.064	2.858AspSer: 2.858 ± 0.069	2.499AspThr: 2.499 ± 0.062	3.149AspVal: 3.149 ± 0.079	0.728AspTrp: 0.728 ± 0.036	2.023AspTyr: 2.023 ± 0.059	0.014AspXaa: 0.014 ± 0.005
Glu	4.432GluAla: 4.432 ± 0.092	0.501GluCys: 0.501 ± 0.023	2.174GluAsp: 2.174 ± 0.056	3.359GluGlu: 3.359 ± 0.08	2.113GluPhe: 2.113 ± 0.059	3.089GluGly: 3.089 ± 0.078	1.432GluHis: 1.432 ± 0.047	4.604GluIle: 4.604 ± 0.097	5.198GluLys: 5.198 ± 0.106	6.17GluLeu: 6.17 ± 0.101	1.686GluMet: 1.686 ± 0.045	3.054GluAsn: 3.054 ± 0.064	1.685GluPro: 1.685 ± 0.055	3.665GluGln: 3.665 ± 0.079	3.625GluArg: 3.625 ± 0.094	3.0GluSer: 3.0 ± 0.072	3.003GluThr: 3.003 ± 0.064	3.529GluVal: 3.529 ± 0.073	0.651GluTrp: 0.651 ± 0.032	1.598GluTyr: 1.598 ± 0.046	0.012GluXaa: 0.012 ± 0.005
Phe	3.05PheAla: 3.05 ± 0.077	0.619PheCys: 0.619 ± 0.03	2.334PheAsp: 2.334 ± 0.055	1.987PheGlu: 1.987 ± 0.056	1.941PhePhe: 1.941 ± 0.065	2.71PheGly: 2.71 ± 0.072	0.96PheHis: 0.96 ± 0.038	3.311PheIle: 3.311 ± 0.079	2.026PheLys: 2.026 ± 0.055	3.593PheLeu: 3.593 ± 0.092	1.004PheMet: 1.004 ± 0.039	2.272PheAsn: 2.272 ± 0.055	1.418PhePro: 1.418 ± 0.049	1.21PheGln: 1.21 ± 0.04	1.771PheArg: 1.771 ± 0.052	3.334PheSer: 3.334 ± 0.082	2.345PheThr: 2.345 ± 0.058	2.196PheVal: 2.196 ± 0.058	0.565PheTrp: 0.565 ± 0.032	1.533PheTyr: 1.533 ± 0.048	0.01PheXaa: 0.01 ± 0.004
Gly	4.325GlyAla: 4.325 ± 0.102	0.897GlyCys: 0.897 ± 0.031	2.91GlyAsp: 2.91 ± 0.072	3.698GlyGlu: 3.698 ± 0.076	2.917GlyPhe: 2.917 ± 0.073	4.122GlyGly: 4.122 ± 0.09	1.497GlyHis: 1.497 ± 0.044	5.272GlyIle: 5.272 ± 0.103	4.526GlyLys: 4.526 ± 0.094	6.423GlyLeu: 6.423 ± 0.118	1.783GlyMet: 1.783 ± 0.056	2.71GlyAsn: 2.71 ± 0.068	1.225GlyPro: 1.225 ± 0.045	2.762GlyGln: 2.762 ± 0.069	3.297GlyArg: 3.297 ± 0.079	3.502GlySer: 3.502 ± 0.066	2.915GlyThr: 2.915 ± 0.063	4.068GlyVal: 4.068 ± 0.091	0.96GlyTrp: 0.96 ± 0.037	2.355GlyTyr: 2.355 ± 0.055	0.015GlyXaa: 0.015 ± 0.005
His	1.61HisAla: 1.61 ± 0.049	0.414HisCys: 0.414 ± 0.026	1.138HisAsp: 1.138 ± 0.041	1.225HisGlu: 1.225 ± 0.047	1.097HisPhe: 1.097 ± 0.045	1.451HisGly: 1.451 ± 0.042	0.739HisHis: 0.739 ± 0.036	1.661HisIle: 1.661 ± 0.048	1.192HisLys: 1.192 ± 0.041	2.362HisLeu: 2.362 ± 0.052	0.492HisMet: 0.492 ± 0.023	0.968HisAsn: 0.968 ± 0.032	1.192HisPro: 1.192 ± 0.044	1.254HisGln: 1.254 ± 0.046	1.336HisArg: 1.336 ± 0.049	1.27HisSer: 1.27 ± 0.046	1.033HisThr: 1.033 ± 0.036	1.062HisVal: 1.062 ± 0.044	0.386HisTrp: 0.386 ± 0.021	0.979HisTyr: 0.979 ± 0.037	0.007HisXaa: 0.007 ± 0.003
Ile	7.056IleAla: 7.056 ± 0.102	0.821IleCys: 0.821 ± 0.032	4.605IleAsp: 4.605 ± 0.089	4.773IleGlu: 4.773 ± 0.085	2.674IlePhe: 2.674 ± 0.076	4.897IleGly: 4.897 ± 0.094	1.421IleHis: 1.421 ± 0.046	5.516IleIle: 5.516 ± 0.104	4.698IleLys: 4.698 ± 0.086	6.47IleLeu: 6.47 ± 0.109	1.661IleMet: 1.661 ± 0.055	4.422IleAsn: 4.422 ± 0.081	3.096IlePro: 3.096 ± 0.063	2.754IleGln: 2.754 ± 0.064	3.563IleArg: 3.563 ± 0.083	5.128IleSer: 5.128 ± 0.105	4.595IleThr: 4.595 ± 0.072	4.332IleVal: 4.332 ± 0.077	0.806IleTrp: 0.806 ± 0.037	2.316IleTyr: 2.316 ± 0.053	0.006IleXaa: 0.006 ± 0.002
Lys	4.948LysAla: 4.948 ± 0.098	0.39LysCys: 0.39 ± 0.025	2.88LysAsp: 2.88 ± 0.069	3.697LysGlu: 3.697 ± 0.077	1.764LysPhe: 1.764 ± 0.056	3.72LysGly: 3.72 ± 0.069	1.335LysHis: 1.335 ± 0.046	4.792LysIle: 4.792 ± 0.088	4.791LysLys: 4.791 ± 0.094	6.31LysLeu: 6.31 ± 0.099	1.792LysMet: 1.792 ± 0.054	3.644LysAsn: 3.644 ± 0.074	2.539LysPro: 2.539 ± 0.067	3.614LysGln: 3.614 ± 0.077	3.603LysArg: 3.603 ± 0.071	3.723LysSer: 3.723 ± 0.072	3.733LysThr: 3.733 ± 0.079	3.633LysVal: 3.633 ± 0.081	0.598LysTrp: 0.598 ± 0.031	1.746LysTyr: 1.746 ± 0.05	0.011LysXaa: 0.011 ± 0.006
Leu	9.303LeuAla: 9.303 ± 0.127	1.189LeuCys: 1.189 ± 0.036	5.152LeuAsp: 5.152 ± 0.091	5.421LeuGlu: 5.421 ± 0.086	4.252LeuPhe: 4.252 ± 0.089	5.808LeuGly: 5.808 ± 0.095	2.082LeuHis: 2.082 ± 0.057	7.412LeuIle: 7.412 ± 0.119	6.138LeuLys: 6.138 ± 0.118	10.964LeuLeu: 10.964 ± 0.161	2.658LeuMet: 2.658 ± 0.06	5.073LeuAsn: 5.073 ± 0.087	5.257LeuPro: 5.257 ± 0.111	4.0LeuGln: 4.0 ± 0.087	5.16LeuArg: 5.16 ± 0.089	7.682LeuSer: 7.682 ± 0.112	6.451LeuThr: 6.451 ± 0.104	5.774LeuVal: 5.774 ± 0.089	1.194LeuTrp: 1.194 ± 0.042	2.903LeuTyr: 2.903 ± 0.06	0.015LeuXaa: 0.015 ± 0.006
Met	2.459MetAla: 2.459 ± 0.057	0.179MetCys: 0.179 ± 0.015	1.151MetAsp: 1.151 ± 0.034	1.223MetGlu: 1.223 ± 0.04	0.735MetPhe: 0.735 ± 0.036	1.649MetGly: 1.649 ± 0.046	0.45MetHis: 0.45 ± 0.024	1.71MetIle: 1.71 ± 0.057	1.735MetLys: 1.735 ± 0.053	2.815MetLeu: 2.815 ± 0.073	0.773MetMet: 0.773 ± 0.038	1.214MetAsn: 1.214 ± 0.038	1.119MetPro: 1.119 ± 0.041	1.23MetGln: 1.23 ± 0.04	1.311MetArg: 1.311 ± 0.038	1.686MetSer: 1.686 ± 0.04	1.782MetThr: 1.782 ± 0.054	1.623MetVal: 1.623 ± 0.053	0.205MetTrp: 0.205 ± 0.017	0.529MetTyr: 0.529 ± 0.029	0.003MetXaa: 0.003 ± 0.002
Asn	3.571AsnAla: 3.571 ± 0.078	0.485AsnCys: 0.485 ± 0.023	2.47AsnAsp: 2.47 ± 0.06	2.732AsnGlu: 2.732 ± 0.074	1.828AsnPhe: 1.828 ± 0.05	3.031AsnGly: 3.031 ± 0.067	1.221AsnHis: 1.221 ± 0.041	3.94AsnIle: 3.94 ± 0.083	3.311AsnLys: 3.311 ± 0.074	4.6AsnLeu: 4.6 ± 0.086	1.176AsnMet: 1.176 ± 0.047	2.967AsnAsn: 2.967 ± 0.075	2.146AsnPro: 2.146 ± 0.056	2.571AsnGln: 2.571 ± 0.071	2.459AsnArg: 2.459 ± 0.054	2.892AsnSer: 2.892 ± 0.074	2.656AsnThr: 2.656 ± 0.067	2.395AsnVal: 2.395 ± 0.058	0.684AsnTrp: 0.684 ± 0.034	1.685AsnTyr: 1.685 ± 0.051	0.007AsnXaa: 0.007 ± 0.003
Pro	2.969ProAla: 2.969 ± 0.066	0.402ProCys: 0.402 ± 0.024	2.412ProAsp: 2.412 ± 0.062	2.955ProGlu: 2.955 ± 0.061	1.725ProPhe: 1.725 ± 0.048	1.981ProGly: 1.981 ± 0.059	0.854ProHis: 0.854 ± 0.035	2.779ProIle: 2.779 ± 0.065	2.159ProLys: 2.159 ± 0.058	4.111ProLeu: 4.111 ± 0.077	0.908ProMet: 0.908 ± 0.035	1.804ProAsn: 1.804 ± 0.048	1.313ProPro: 1.313 ± 0.051	1.692ProGln: 1.692 ± 0.048	1.509ProArg: 1.509 ± 0.049	2.208ProSer: 2.208 ± 0.055	2.289ProThr: 2.289 ± 0.06	2.783ProVal: 2.783 ± 0.072	0.49ProTrp: 0.49 ± 0.028	1.367ProTyr: 1.367 ± 0.045	0.012ProXaa: 0.012 ± 0.007
Gln	4.026GlnAla: 4.026 ± 0.082	0.439GlnCys: 0.439 ± 0.026	1.858GlnAsp: 1.858 ± 0.056	2.422GlnGlu: 2.422 ± 0.067	1.817GlnPhe: 1.817 ± 0.051	2.825GlnGly: 2.825 ± 0.066	1.373GlnHis: 1.373 ± 0.045	3.547GlnIle: 3.547 ± 0.073	2.867GlnLys: 2.867 ± 0.059	5.368GlnLeu: 5.368 ± 0.124	1.123GlnMet: 1.123 ± 0.042	1.933GlnAsn: 1.933 ± 0.06	1.879GlnPro: 1.879 ± 0.058	3.686GlnGln: 3.686 ± 0.089	2.696GlnArg: 2.696 ± 0.064	2.682GlnSer: 2.682 ± 0.067	2.357GlnThr: 2.357 ± 0.059	2.718GlnVal: 2.718 ± 0.054	0.623GlnTrp: 0.623 ± 0.032	1.462GlnTyr: 1.462 ± 0.041	0.003GlnXaa: 0.003 ± 0.002
Arg	3.661ArgAla: 3.661 ± 0.08	0.62ArgCys: 0.62 ± 0.031	2.467ArgAsp: 2.467 ± 0.062	3.319ArgGlu: 3.319 ± 0.078	2.391ArgPhe: 2.391 ± 0.056	2.717ArgGly: 2.717 ± 0.063	1.387ArgHis: 1.387 ± 0.045	3.806ArgIle: 3.806 ± 0.071	3.459ArgLys: 3.459 ± 0.071	5.857ArgLeu: 5.857 ± 0.087	1.253ArgMet: 1.253 ± 0.04	2.289ArgAsn: 2.289 ± 0.048	1.865ArgPro: 1.865 ± 0.052	3.009ArgGln: 3.009 ± 0.073	3.132ArgArg: 3.132 ± 0.08	2.563ArgSer: 2.563 ± 0.064	2.361ArgThr: 2.361 ± 0.052	2.873ArgVal: 2.873 ± 0.073	0.811ArgTrp: 0.811 ± 0.037	2.077ArgTyr: 2.077 ± 0.058	0.014ArgXaa: 0.014 ± 0.004
Ser	4.872SerAla: 4.872 ± 0.067	0.678SerCys: 0.678 ± 0.033	3.086SerAsp: 3.086 ± 0.072	3.564SerGlu: 3.564 ± 0.079	2.449SerPhe: 2.449 ± 0.054	4.469SerGly: 4.469 ± 0.076	1.657SerHis: 1.657 ± 0.052	4.161SerIle: 4.161 ± 0.08	3.119SerLys: 3.119 ± 0.062	6.715SerLeu: 6.715 ± 0.094	1.472SerMet: 1.472 ± 0.048	2.537SerAsn: 2.537 ± 0.07	2.501SerPro: 2.501 ± 0.064	3.011SerGln: 3.011 ± 0.063	3.092SerArg: 3.092 ± 0.066	3.7SerSer: 3.7 ± 0.096	3.146SerThr: 3.146 ± 0.072	3.954SerVal: 3.954 ± 0.077	0.745SerTrp: 0.745 ± 0.033	1.877SerTyr: 1.877 ± 0.054	0.006SerXaa: 0.006 ± 0.003
Thr	4.44ThrAla: 4.44 ± 0.081	0.474ThrCys: 0.474 ± 0.026	2.891ThrAsp: 2.891 ± 0.063	3.154ThrGlu: 3.154 ± 0.069	2.078ThrPhe: 2.078 ± 0.059	3.899ThrGly: 3.899 ± 0.077	1.311ThrHis: 1.311 ± 0.044	3.903ThrIle: 3.903 ± 0.091	2.812ThrLys: 2.812 ± 0.06	6.602ThrLeu: 6.602 ± 0.105	1.08ThrMet: 1.08 ± 0.041	2.269ThrAsn: 2.269 ± 0.06	2.777ThrPro: 2.777 ± 0.065	2.445ThrGln: 2.445 ± 0.069	2.646ThrArg: 2.646 ± 0.056	3.038ThrSer: 3.038 ± 0.066	3.093ThrThr: 3.093 ± 0.066	3.574ThrVal: 3.574 ± 0.075	0.583ThrTrp: 0.583 ± 0.033	1.404ThrTyr: 1.404 ± 0.05	0.011ThrXaa: 0.011 ± 0.004
Val	5.054ValAla: 5.054 ± 0.081	0.644ValCys: 0.644 ± 0.028	3.151ValAsp: 3.151 ± 0.065	3.646ValGlu: 3.646 ± 0.081	2.391ValPhe: 2.391 ± 0.064	3.686ValGly: 3.686 ± 0.086	0.969ValHis: 0.969 ± 0.041	4.965ValIle: 4.965 ± 0.1	3.943ValLys: 3.943 ± 0.076	5.508ValLeu: 5.508 ± 0.087	1.756ValMet: 1.756 ± 0.053	3.028ValAsn: 3.028 ± 0.065	2.003ValPro: 2.003 ± 0.053	1.841ValGln: 1.841 ± 0.048	2.993ValArg: 2.993 ± 0.068	3.867ValSer: 3.867 ± 0.089	3.82ValThr: 3.82 ± 0.076	3.857ValVal: 3.857 ± 0.08	0.672ValTrp: 0.672 ± 0.033	1.643ValTyr: 1.643 ± 0.048	0.007ValXaa: 0.007 ± 0.003
Trp	0.753TrpAla: 0.753 ± 0.03	0.184TrpCys: 0.184 ± 0.015	0.472TrpAsp: 0.472 ± 0.023	0.464TrpGlu: 0.464 ± 0.026	0.637TrpPhe: 0.637 ± 0.028	0.708TrpGly: 0.708 ± 0.032	0.343TrpHis: 0.343 ± 0.023	0.853TrpIle: 0.853 ± 0.036	0.505TrpLys: 0.505 ± 0.027	1.994TrpLeu: 1.994 ± 0.057	0.309TrpMet: 0.309 ± 0.021	0.486TrpAsn: 0.486 ± 0.026	0.489TrpPro: 0.489 ± 0.025	1.102TrpGln: 1.102 ± 0.038	0.788TrpArg: 0.788 ± 0.034	0.705TrpSer: 0.705 ± 0.03	0.397TrpThr: 0.397 ± 0.024	0.825TrpVal: 0.825 ± 0.031	0.177TrpTrp: 0.177 ± 0.012	0.382TrpTyr: 0.382 ± 0.023	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.257TyrAla: 2.257 ± 0.058	0.526TyrCys: 0.526 ± 0.031	1.591TyrAsp: 1.591 ± 0.05	1.44TyrGlu: 1.44 ± 0.048	1.533TyrPhe: 1.533 ± 0.052	2.078TyrGly: 2.078 ± 0.057	1.054TyrHis: 1.054 ± 0.038	1.973TyrIle: 1.973 ± 0.055	1.408TyrLys: 1.408 ± 0.049	3.587TyrLeu: 3.587 ± 0.072	0.619TyrMet: 0.619 ± 0.031	1.31TyrAsn: 1.31 ± 0.042	1.472TyrPro: 1.472 ± 0.051	2.199TyrGln: 2.199 ± 0.057	2.034TyrArg: 2.034 ± 0.054	2.038TyrSer: 2.038 ± 0.05	1.396TyrThr: 1.396 ± 0.047	1.623TyrVal: 1.623 ± 0.044	0.522TyrTrp: 0.522 ± 0.028	1.025TyrTyr: 1.025 ± 0.045	0.006TyrXaa: 0.006 ± 0.003
Xaa	0.012XaaAla: 0.012 ± 0.004	0.003XaaCys: 0.003 ± 0.002	0.011XaaAsp: 0.011 ± 0.004	0.011XaaGlu: 0.011 ± 0.006	0.007XaaPhe: 0.007 ± 0.003	0.004XaaGly: 0.004 ± 0.003	0.004XaaHis: 0.004 ± 0.002	0.004XaaIle: 0.004 ± 0.002	0.015XaaLys: 0.015 ± 0.005	0.021XaaLeu: 0.021 ± 0.008	0.006XaaMet: 0.006 ± 0.003	0.001XaaAsn: 0.001 ± 0.001	0.011XaaPro: 0.011 ± 0.003	0.014XaaGln: 0.014 ± 0.005	0.014XaaArg: 0.014 ± 0.004	0.012XaaSer: 0.012 ± 0.005	0.007XaaThr: 0.007 ± 0.003	0.007XaaVal: 0.007 ± 0.003	0.004XaaTrp: 0.004 ± 0.002	0.003XaaTyr: 0.003 ± 0.002	0.024XaaXaa: 0.024 ± 0.007

Statistics based on 3133 proteins (722245 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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