Capsicum annuum (Capsicum pepper)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae; Capsicum

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35548 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G3AJW8|A0A2G3AJW8_CAPAN Uncharacterized protein OS=Capsicum annuum OX=4072 GN=T459_02360 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 5.23GDD4 pKa = 3.8EE5 pKa = 4.87SEE7 pKa = 4.17SDD9 pKa = 3.27NYY11 pKa = 10.98FEE13 pKa = 4.51TFVPNLYY20 pKa = 10.43YY21 pKa = 10.83LEE23 pKa = 4.94LSHH26 pKa = 7.42DD27 pKa = 3.81FHH29 pKa = 8.21DD30 pKa = 5.64FKK32 pKa = 11.23CRR34 pKa = 11.84PADD37 pKa = 3.33VSSVVNAKK45 pKa = 10.34DD46 pKa = 3.96NPDD49 pKa = 3.38QGSDD53 pKa = 3.49DD54 pKa = 5.18DD55 pKa = 5.37EE56 pKa = 4.61EE57 pKa = 4.47NSCNEE62 pKa = 3.82HH63 pKa = 6.42HH64 pKa = 6.8QDD66 pKa = 4.41SDD68 pKa = 4.51DD69 pKa = 4.54EE70 pKa = 4.53EE71 pKa = 5.52DD72 pKa = 3.51SCSDD76 pKa = 3.82YY77 pKa = 11.16FPDD80 pKa = 4.09SHH82 pKa = 9.04DD83 pKa = 3.78EE84 pKa = 4.47EE85 pKa = 4.35EE86 pKa = 4.82DD87 pKa = 3.12NWGEE91 pKa = 3.8YY92 pKa = 9.5HH93 pKa = 6.84QDD95 pKa = 3.41SEE97 pKa = 4.31EE98 pKa = 4.47DD99 pKa = 3.23SWGDD103 pKa = 3.5YY104 pKa = 10.3YY105 pKa = 11.3QDD107 pKa = 4.41SDD109 pKa = 4.97EE110 pKa = 5.04EE111 pKa = 5.1DD112 pKa = 4.27DD113 pKa = 5.83NADD116 pKa = 3.4QDD118 pKa = 3.98SDD120 pKa = 4.04EE121 pKa = 6.13EE122 pKa = 4.33DD123 pKa = 3.55MQWYY127 pKa = 9.65SVASEE132 pKa = 4.43MVCSTRR138 pKa = 11.84MSSLLALKK146 pKa = 10.54RR147 pKa = 11.84GGCFFCWCFGMEE159 pKa = 4.24VVVGSGSDD167 pKa = 3.2KK168 pKa = 10.98QSIICEE174 pKa = 4.31DD175 pKa = 4.33PNNMILIGVARR186 pKa = 11.84RR187 pKa = 11.84QGKK190 pKa = 10.23SIDD193 pKa = 3.74DD194 pKa = 3.87GFEE197 pKa = 3.89GVIKK201 pKa = 10.81DD202 pKa = 3.46KK203 pKa = 11.61GNLVKK208 pKa = 10.79VPTIVKK214 pKa = 7.61YY215 pKa = 9.19TSLISIVHH223 pKa = 6.04IPFLNTSTVVPAA235 pKa = 4.63

Molecular weight: 26.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G2ZIX5|A0A2G2ZIX5_CAPAN Uncharacterized protein OS=Capsicum annuum OX=4072 GN=T459_14957 PE=4 SV=1
MM1 pKa = 7.62DD2 pKa = 3.54RR3 pKa = 11.84WMDD6 pKa = 2.84RR7 pKa = 11.84WMGRR11 pKa = 11.84WMGRR15 pKa = 11.84RR16 pKa = 11.84MGRR19 pKa = 11.84RR20 pKa = 11.84MGRR23 pKa = 11.84RR24 pKa = 11.84MGRR27 pKa = 11.84RR28 pKa = 11.84MGRR31 pKa = 11.84RR32 pKa = 11.84MGRR35 pKa = 11.84RR36 pKa = 11.84MGRR39 pKa = 11.84RR40 pKa = 11.84MGRR43 pKa = 11.84RR44 pKa = 11.84MGRR47 pKa = 11.84RR48 pKa = 11.84MGRR51 pKa = 11.84RR52 pKa = 11.84MGRR55 pKa = 11.84RR56 pKa = 11.84MGRR59 pKa = 11.84RR60 pKa = 11.84MGRR63 pKa = 11.84RR64 pKa = 11.84MGRR67 pKa = 11.84RR68 pKa = 11.84MGRR71 pKa = 11.84RR72 pKa = 11.84MGRR75 pKa = 11.84RR76 pKa = 11.84MGRR79 pKa = 11.84RR80 pKa = 11.84MGRR83 pKa = 11.84RR84 pKa = 11.84MGRR87 pKa = 11.84RR88 pKa = 11.84MGRR91 pKa = 11.84WMGRR95 pKa = 11.84RR96 pKa = 11.84MDD98 pKa = 3.47RR99 pKa = 3.67

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35548

0

35548

12948852

25

5452

364.3

40.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.229 ± 0.014

1.9 ± 0.006

5.418 ± 0.01

6.487 ± 0.016

4.293 ± 0.01

6.279 ± 0.013

2.368 ± 0.005

5.708 ± 0.01

6.425 ± 0.013

9.903 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.005

4.735 ± 0.01

4.626 ± 0.013

3.574 ± 0.008

5.029 ± 0.009

8.746 ± 0.016

4.885 ± 0.008

6.575 ± 0.011

1.275 ± 0.005

2.999 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski