Murinocardiopsis flavida

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Murinocardiopsis

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P8D114|A0A2P8D114_9ACTN Hypoxanthine phosphoribosyltransferase OS=Murinocardiopsis flavida OX=645275 GN=CLV63_12129 PE=3 SV=1
MM1 pKa = 7.59KK2 pKa = 10.47KK3 pKa = 10.22LFAAGAFTATVAAGVLFAGAPAFADD28 pKa = 3.74DD29 pKa = 4.97NEE31 pKa = 4.76TNGDD35 pKa = 3.79GAVLSGNQVDD45 pKa = 3.93VDD47 pKa = 3.52INTAVNVCGNAVAVVGVANGNCGNAGATVVNHH79 pKa = 6.44YY80 pKa = 11.31DD81 pKa = 3.46DD82 pKa = 4.07

Molecular weight: 7.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P8CW00|A0A2P8CW00_9ACTN Uncharacterized protein DUF1116 OS=Murinocardiopsis flavida OX=645275 GN=CLV63_12721 PE=4 SV=1
MM1 pKa = 7.39WPPRR5 pKa = 11.84IAGGTAVGTAAGSAVHH21 pKa = 6.95RR22 pKa = 11.84GPIGPRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84PSAIRR35 pKa = 11.84LRR37 pKa = 11.84PVVGAGGFAAAARR50 pKa = 11.84ALSVV54 pKa = 3.15

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6614

0

6614

2111645

29

6142

319.3

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.549 ± 0.051

0.733 ± 0.008

6.308 ± 0.028

5.617 ± 0.026

2.792 ± 0.017

9.732 ± 0.035

2.254 ± 0.015

3.438 ± 0.022

1.732 ± 0.02

10.096 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.86 ± 0.013

1.639 ± 0.012

6.269 ± 0.029

2.396 ± 0.017

8.27 ± 0.031

4.886 ± 0.021

5.681 ± 0.019

8.343 ± 0.028

1.468 ± 0.011

1.939 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski