Mesonia algae
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2856 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W7HW89|A0A2W7HW89_9FLAO DNA helicase OS=Mesonia algae OX=213248 GN=LX95_02870 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 9.28 KK3 pKa = 8.54 TYY5 pKa = 10.72 LSILIALISITSFAQEE21 pKa = 3.96 ALITGYY27 pKa = 10.66 VDD29 pKa = 4.1 SPCSGANGRR38 pKa = 11.84 VLEE41 pKa = 4.38 IYY43 pKa = 11.17 VNGTIDD49 pKa = 3.52 FTGWNIQRR57 pKa = 11.84 QSNGGGYY64 pKa = 7.78 STNIDD69 pKa = 3.49 LSSFATISDD78 pKa = 3.52 DD79 pKa = 5.77 FIYY82 pKa = 9.52 LTNDD86 pKa = 3.11 QAILNAEE93 pKa = 4.19 FGITANVIEE102 pKa = 4.62 SSVISSNGNDD112 pKa = 3.16 AFQIIDD118 pKa = 3.74 NNSVVIDD125 pKa = 3.76 RR126 pKa = 11.84 FGEE129 pKa = 4.1 EE130 pKa = 4.37 NIDD133 pKa = 3.71 GAGTAWEE140 pKa = 4.68 HH141 pKa = 6.37 LDD143 pKa = 4.52 SYY145 pKa = 9.99 YY146 pKa = 11.02 LRR148 pKa = 11.84 NNSSIANAGSFDD160 pKa = 3.79 VNNWTFGALNSLDD173 pKa = 3.71 NQGLCNSSSALSTMVNLGSFTPVVTTVAEE202 pKa = 4.14 IEE204 pKa = 3.94 FDD206 pKa = 3.26 EE207 pKa = 5.34 AYY209 pKa = 9.78 ISAVEE214 pKa = 4.22 GSGTATLSVTISEE227 pKa = 5.02 LPNSDD232 pKa = 3.88 ANIDD236 pKa = 3.58 VAILVNEE243 pKa = 4.08 STAIDD248 pKa = 3.9 NQHH251 pKa = 4.75 YY252 pKa = 8.7 TYY254 pKa = 10.9 TNEE257 pKa = 4.0 TLTFTTTGSLSQSITITIPDD277 pKa = 3.93 NFDD280 pKa = 3.58 AEE282 pKa = 4.61 ADD284 pKa = 3.68 TFLALEE290 pKa = 4.23 LTNPVNANLGEE301 pKa = 4.79 DD302 pKa = 3.73 NLSVVYY308 pKa = 10.56 ILDD311 pKa = 4.01 NEE313 pKa = 4.26 AHH315 pKa = 6.44 SPIATQNLDD324 pKa = 3.21 INFATSYY331 pKa = 10.88 AITGPNPGSEE341 pKa = 3.89 IVAHH345 pKa = 6.86 DD346 pKa = 3.74 ATTEE350 pKa = 3.89 RR351 pKa = 11.84 LFVMNSGNASVEE363 pKa = 3.91 ILDD366 pKa = 4.23 FSSPLNITSISTIDD380 pKa = 3.45 LSSYY384 pKa = 10.61 GDD386 pKa = 3.33 SSTSVAVHH394 pKa = 6.38 NGLVAATAVPNDD406 pKa = 3.34 KK407 pKa = 10.66 NLNGKK412 pKa = 7.96 VVFMDD417 pKa = 3.52 TNGVIITSLEE427 pKa = 4.21 VGSLPDD433 pKa = 4.48 MITFSPDD440 pKa = 2.53 GNMLLVANEE449 pKa = 4.39 GEE451 pKa = 4.3 PNSDD455 pKa = 3.33 YY456 pKa = 11.21 TIDD459 pKa = 3.77 PEE461 pKa = 4.49 GTISIIDD468 pKa = 3.78 LSIATANLTQANVTTLNFNAFDD490 pKa = 3.83 TQEE493 pKa = 4.21 TQLKK497 pKa = 9.62 ADD499 pKa = 3.94 GVRR502 pKa = 11.84 IFGPNATVSQDD513 pKa = 3.74 LEE515 pKa = 4.14 PEE517 pKa = 4.42 YY518 pKa = 10.37 ITVSQDD524 pKa = 2.49 NTTAWVSLQEE534 pKa = 4.06 NNAIAVVDD542 pKa = 4.43 LTTMQITNILPLGLKK557 pKa = 10.03 DD558 pKa = 4.43 HH559 pKa = 6.77 SLPNNALDD567 pKa = 3.84 TSNEE571 pKa = 3.49 QDD573 pKa = 4.31 FIFMANWPIKK583 pKa = 9.56 GMYY586 pKa = 8.77 MPDD589 pKa = 4.13 AIASYY594 pKa = 10.4 SVNGNTYY601 pKa = 9.88 IVTANEE607 pKa = 3.7 GDD609 pKa = 3.3 ARR611 pKa = 11.84 EE612 pKa = 3.91 YY613 pKa = 10.67 DD614 pKa = 3.6 TFEE617 pKa = 4.23 EE618 pKa = 4.52 EE619 pKa = 4.79 VNIEE623 pKa = 4.74 DD624 pKa = 3.55 IVLDD628 pKa = 3.68 ASVFPNQDD636 pKa = 3.06 FLEE639 pKa = 4.97 LDD641 pKa = 3.7 EE642 pKa = 4.99 NLGKK646 pKa = 10.31 IKK648 pKa = 9.35 ITSEE652 pKa = 4.02 FGDD655 pKa = 3.45 IDD657 pKa = 3.88 NDD659 pKa = 4.22 GEE661 pKa = 4.13 FEE663 pKa = 4.31 EE664 pKa = 4.39 IHH666 pKa = 6.1 VFGGRR671 pKa = 11.84 SFSIYY676 pKa = 10.51 DD677 pKa = 4.77 AITGAQVYY685 pKa = 10.54 DD686 pKa = 3.59 SGSDD690 pKa = 3.42 FEE692 pKa = 5.74 RR693 pKa = 11.84 IIAEE697 pKa = 4.06 DD698 pKa = 3.55 PVYY701 pKa = 10.99 NAIFNATDD709 pKa = 4.27 DD710 pKa = 3.99 EE711 pKa = 4.8 NEE713 pKa = 4.08 LKK715 pKa = 10.75 NRR717 pKa = 11.84 SDD719 pKa = 3.58 NKK721 pKa = 10.23 GPEE724 pKa = 3.98 PEE726 pKa = 3.86 AVIVQEE732 pKa = 4.3 IDD734 pKa = 3.06 GTFYY738 pKa = 11.49 AFIALEE744 pKa = 4.06 RR745 pKa = 11.84 VGGFMVYY752 pKa = 10.47 DD753 pKa = 3.68 ITNPNTPVFEE763 pKa = 5.01 GYY765 pKa = 10.42 FNNRR769 pKa = 11.84 SVTPGEE775 pKa = 3.98 GDD777 pKa = 3.51 LANLGDD783 pKa = 4.7 LAPEE787 pKa = 4.38 SIVYY791 pKa = 9.66 IAPEE795 pKa = 3.79 NNAEE799 pKa = 4.1 NKK801 pKa = 10.15 GLIVIANEE809 pKa = 3.7 VSATISVYY817 pKa = 8.27 TLEE820 pKa = 4.51 NNVLTTEE827 pKa = 4.04 SFEE830 pKa = 4.42 VNNSFVVYY838 pKa = 8.74 PNPATSSRR846 pKa = 11.84 VFFAQPTTYY855 pKa = 10.61 EE856 pKa = 4.23 LYY858 pKa = 10.44 DD859 pKa = 3.27 IQGRR863 pKa = 11.84 KK864 pKa = 7.94 VKK866 pKa = 10.17 AAQNSTFINVDD877 pKa = 3.19 GLNAGTYY884 pKa = 8.76 IVKK887 pKa = 10.35 NDD889 pKa = 3.33 GGQTQKK895 pKa = 11.4 LIIKK899 pKa = 9.66
Molecular weight: 97.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.706
IPC_protein 3.732
Toseland 3.516
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.554
Grimsley 3.414
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.554
EMBOSS 3.63
Sillero 3.846
Patrickios 1.494
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|A0A2W7JUA2|A0A2W7JUA2_9FLAO Uncharacterized protein OS=Mesonia algae OX=213248 GN=LX95_02170 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.15 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 KK13 pKa = 8.29 NKK15 pKa = 9.34 HH16 pKa = 4.03 GFRR19 pKa = 11.84 EE20 pKa = 4.05 RR21 pKa = 11.84 MASVNGRR28 pKa = 11.84 KK29 pKa = 9.21 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.73 LSVSSEE47 pKa = 3.76 NRR49 pKa = 11.84 HH50 pKa = 4.47 KK51 pKa = 10.5 HH52 pKa = 4.49
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2856
0
2856
933224
40
2403
326.8
37.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.09 ± 0.041
0.71 ± 0.016
5.351 ± 0.041
7.24 ± 0.046
5.262 ± 0.035
6.062 ± 0.045
1.798 ± 0.024
8.009 ± 0.041
8.133 ± 0.074
9.504 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.103 ± 0.025
6.241 ± 0.055
3.268 ± 0.022
3.763 ± 0.032
3.244 ± 0.033
6.559 ± 0.042
5.569 ± 0.042
6.029 ± 0.034
0.976 ± 0.018
4.09 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here