Marinomonas pollencensis
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3539 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3E0DH82|A0A3E0DH82_9GAMM SOS response UmuD protein OS=Marinomonas pollencensis OX=491954 GN=DFP81_11125 PE=3 SV=1
MM1 pKa = 7.47 KK2 pKa = 9.6 PYY4 pKa = 10.57 IMNYY8 pKa = 9.9 SEE10 pKa = 4.77 TIEE13 pKa = 3.96 ISPVCSNWRR22 pKa = 11.84 KK23 pKa = 9.71 IDD25 pKa = 3.58 TTTKK29 pKa = 8.59 TFTIEE34 pKa = 3.78 SHH36 pKa = 7.24 DD37 pKa = 3.88 NDD39 pKa = 5.01 EE40 pKa = 4.46 IHH42 pKa = 7.4 CDD44 pKa = 2.78 WTVQTNVTEE53 pKa = 4.21 PTDD56 pKa = 3.62 NDD58 pKa = 4.33 SILCLDD64 pKa = 3.6 EE65 pKa = 3.98 TAITKK70 pKa = 10.38 SLEE73 pKa = 4.13 PTDD76 pKa = 4.14 QDD78 pKa = 5.12 CMHH81 pKa = 7.32 LDD83 pKa = 3.14 ATYY86 pKa = 7.2 EE87 pKa = 4.07 TRR89 pKa = 11.84 VVEE92 pKa = 4.22 TSDD95 pKa = 3.34 NDD97 pKa = 4.38 SIYY100 pKa = 11.09 LDD102 pKa = 3.62 TTVMTEE108 pKa = 4.2 SIEE111 pKa = 4.38 GLDD114 pKa = 3.47 PDD116 pKa = 4.17 EE117 pKa = 4.71 TVLAHH122 pKa = 7.09
Molecular weight: 13.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.884
IPC_protein 3.859
Toseland 3.656
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.177
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.986
Patrickios 1.036
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>tr|A0A3E0DM61|A0A3E0DM61_9GAMM D D-heptose 1 7-bisphosphate phosphatase OS=Marinomonas pollencensis OX=491954 GN=DFP81_105217 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.12 SGRR28 pKa = 11.84 QIVARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.91 VLSAA44 pKa = 4.11
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3539
0
3539
1124909
25
1607
317.9
35.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.116 ± 0.047
1.034 ± 0.012
5.396 ± 0.03
6.062 ± 0.043
4.134 ± 0.026
6.793 ± 0.037
2.301 ± 0.019
6.264 ± 0.035
5.172 ± 0.038
10.739 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.634 ± 0.019
3.92 ± 0.028
4.027 ± 0.021
4.569 ± 0.039
4.667 ± 0.034
6.826 ± 0.036
5.197 ± 0.026
6.98 ± 0.037
1.255 ± 0.016
2.915 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here