Trueperella bialowiezensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Trueperella

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1821 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A448PFB6|A0A448PFB6_9ACTO Probable ATP-dependent Clp protease ATP-binding subunit OS=Trueperella bialowiezensis OX=312285 GN=clpC PE=3 SV=1
MM1 pKa = 6.5TTATTRR7 pKa = 11.84KK8 pKa = 9.18RR9 pKa = 11.84QLALALILPVGLALAACEE27 pKa = 4.07SDD29 pKa = 3.22TTIGINEE36 pKa = 4.97DD37 pKa = 3.34GVASITMEE45 pKa = 3.59IHH47 pKa = 6.47DD48 pKa = 4.47TSGMLAAGGMTSCDD62 pKa = 3.42EE63 pKa = 4.35FSSSIAEE70 pKa = 4.26GDD72 pKa = 3.64DD73 pKa = 3.47SFTVEE78 pKa = 5.81DD79 pKa = 3.68ISKK82 pKa = 10.62DD83 pKa = 3.33GNLGCRR89 pKa = 11.84ITGNSGVSAVDD100 pKa = 3.48DD101 pKa = 4.18DD102 pKa = 4.77VLVEE106 pKa = 4.24TDD108 pKa = 3.16DD109 pKa = 4.04TYY111 pKa = 11.5IFTIDD116 pKa = 4.12EE117 pKa = 4.48DD118 pKa = 4.24LGDD121 pKa = 3.9TGLTDD126 pKa = 4.03EE127 pKa = 5.28DD128 pKa = 4.34LAMLKK133 pKa = 10.51QMGFGFSFTVEE144 pKa = 3.93MPGDD148 pKa = 3.45IVKK151 pKa = 10.66ADD153 pKa = 3.53GAQISGNRR161 pKa = 11.84ATFDD165 pKa = 3.75DD166 pKa = 4.76PSVFSQGITVEE177 pKa = 4.88GYY179 pKa = 10.27KK180 pKa = 10.35SGSGGGSKK188 pKa = 8.09PTPSPTATTEE198 pKa = 4.28GPEE201 pKa = 4.41TPTPTEE207 pKa = 4.63DD208 pKa = 3.59PTDD211 pKa = 3.81EE212 pKa = 4.42PTDD215 pKa = 3.84EE216 pKa = 4.24PTEE219 pKa = 4.23APTTNEE225 pKa = 4.32DD226 pKa = 3.31EE227 pKa = 5.46DD228 pKa = 4.27GGFPVWGWILIGVGALAVIGGVVFAVTRR256 pKa = 11.84KK257 pKa = 10.47KK258 pKa = 10.78NDD260 pKa = 3.11GDD262 pKa = 3.56NGGFGGPGYY271 pKa = 9.62PGGPGGPGYY280 pKa = 9.88PGGYY284 pKa = 8.9GQGGYY289 pKa = 9.92GQGGYY294 pKa = 9.92GQGGYY299 pKa = 9.88GQGGQPTQQFAPGQGAPGQGGYY321 pKa = 9.99GQGGYY326 pKa = 9.88GQGGQPTQQFAPTQPLPTGQANYY349 pKa = 9.7GHH351 pKa = 6.68SGHH354 pKa = 7.18PAPRR358 pKa = 11.84NPAGYY363 pKa = 9.58DD364 pKa = 3.22AAPNVPQSPGAQGAYY379 pKa = 9.67GQVGSQGGYY388 pKa = 8.8QSSGQSTPAASSFSRR403 pKa = 11.84GGAEE407 pKa = 3.95GGLNEE412 pKa = 4.65TGNMPKK418 pKa = 9.89QDD420 pKa = 3.26

Molecular weight: 42.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A448PFN7|A0A448PFN7_9ACTO ATP synthase subunit alpha OS=Trueperella bialowiezensis OX=312285 GN=atpA PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1821

0

1821

618937

29

3317

339.9

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.666 ± 0.08

0.595 ± 0.014

6.375 ± 0.054

6.408 ± 0.063

3.182 ± 0.038

8.346 ± 0.047

2.085 ± 0.025

5.278 ± 0.05

3.255 ± 0.04

9.194 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.028

2.858 ± 0.03

5.1 ± 0.049

3.289 ± 0.031

6.357 ± 0.057

5.664 ± 0.041

5.952 ± 0.051

8.395 ± 0.044

1.373 ± 0.024

2.397 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski