Pacific flying fox faeces associated circular DNA virus-5
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140CTT9|A0A140CTT9_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-5 OX=1796014 PE=4 SV=1
MM1 pKa = 7.09 GWRR4 pKa = 11.84 RR5 pKa = 11.84 YY6 pKa = 8.83 HH7 pKa = 5.04 RR8 pKa = 11.84 HH9 pKa = 5.05 SRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 7.64 YY14 pKa = 8.06 HH15 pKa = 7.42 KK16 pKa = 10.45 ARR18 pKa = 11.84 QAYY21 pKa = 10.22 DD22 pKa = 3.03 NFAFFIPHH30 pKa = 5.19 SVIYY34 pKa = 10.31 LALQGNRR41 pKa = 11.84 FPGGGEE47 pKa = 4.01 PIPYY51 pKa = 9.4 YY52 pKa = 10.04 PRR54 pKa = 11.84 VYY56 pKa = 11.1 GNILNFTEE64 pKa = 4.72 PYY66 pKa = 9.94 IGISVTQGAPQLFYY80 pKa = 10.88 FQLNDD85 pKa = 3.13 IPNYY89 pKa = 10.39 LQFTGLYY96 pKa = 8.14 DD97 pKa = 2.99 QYY99 pKa = 10.85 RR100 pKa = 11.84 ISRR103 pKa = 11.84 ITASFFPSHH112 pKa = 6.48 TSQPINNSAYY122 pKa = 9.74 MKK124 pKa = 10.45 TDD126 pKa = 3.48 GTWHH130 pKa = 5.19 QTSDD134 pKa = 3.57 PDD136 pKa = 4.71 AVTEE140 pKa = 4.05 QYY142 pKa = 7.36 TTGVSSHH149 pKa = 6.71 SDD151 pKa = 2.5 SGTYY155 pKa = 10.8 YY156 pKa = 9.79 NTFGDD161 pKa = 4.65 FMMTCWVDD169 pKa = 3.21 VDD171 pKa = 4.21 GTNSSLTQSSYY182 pKa = 10.4 QNVTQLHH189 pKa = 5.65 KK190 pKa = 10.44 WRR192 pKa = 11.84 WTEE195 pKa = 3.57 KK196 pKa = 10.73 HH197 pKa = 6.28 EE198 pKa = 4.17 FTFWPKK204 pKa = 10.44 PYY206 pKa = 10.14 LVSANVGGTTGPAPDD221 pKa = 4.21 LWQDD225 pKa = 3.53 CSDD228 pKa = 4.06 VDD230 pKa = 3.45 IQYY233 pKa = 10.2 WGLWVQALPQLPTGQLYY250 pKa = 10.2 NQTQQYY256 pKa = 7.39 DD257 pKa = 3.66 TTNIMLYY264 pKa = 8.85 CTYY267 pKa = 10.06 HH268 pKa = 6.71 VEE270 pKa = 4.33 FRR272 pKa = 11.84 FRR274 pKa = 11.84 QQ275 pKa = 3.35
Molecular weight: 32.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.054
IPC2_protein 6.084
IPC_protein 6.237
Toseland 6.664
ProMoST 6.576
Dawson 6.59
Bjellqvist 6.532
Wikipedia 6.62
Rodwell 6.59
Grimsley 6.839
Solomon 6.605
Lehninger 6.605
Nozaki 6.854
DTASelect 7.059
Thurlkill 7.088
EMBOSS 7.059
Sillero 6.985
Patrickios 0.693
IPC_peptide 6.62
IPC2_peptide 6.912
IPC2.peptide.svr19 6.859
Protein with the highest isoelectric point:
>tr|A0A140CTT9|A0A140CTT9_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-5 OX=1796014 PE=4 SV=1
MM1 pKa = 7.55 GSRR4 pKa = 11.84 RR5 pKa = 11.84 YY6 pKa = 10.29 VFTIFVSSDD15 pKa = 3.28 RR16 pKa = 11.84 DD17 pKa = 3.56 WITEE21 pKa = 4.18 GIEE24 pKa = 4.1 RR25 pKa = 11.84 QNVKK29 pKa = 10.72 YY30 pKa = 10.41 LVAQQEE36 pKa = 4.53 ICSEE40 pKa = 4.15 TGRR43 pKa = 11.84 LHH45 pKa = 5.03 WQGYY49 pKa = 9.76 AEE51 pKa = 4.17 FKK53 pKa = 10.1 KK54 pKa = 10.48 VCRR57 pKa = 11.84 IPGAKK62 pKa = 9.8 SILGCDD68 pKa = 3.51 WAHH71 pKa = 6.33 LAVPNGDD78 pKa = 2.94 RR79 pKa = 11.84 KK80 pKa = 10.79 ANKK83 pKa = 9.88 DD84 pKa = 3.61 YY85 pKa = 9.52 CTKK88 pKa = 10.29 LASRR92 pKa = 11.84 APGGEE97 pKa = 4.0 SIEE100 pKa = 3.84 IGKK103 pKa = 10.02 FEE105 pKa = 4.68 EE106 pKa = 5.35 GGQGRR111 pKa = 11.84 KK112 pKa = 7.1 STSVAEE118 pKa = 4.06 LRR120 pKa = 11.84 EE121 pKa = 4.3 EE122 pKa = 3.84 IRR124 pKa = 11.84 GGQTTLRR131 pKa = 11.84 QEE133 pKa = 3.95 IVEE136 pKa = 4.08 GRR138 pKa = 11.84 VLNIGAIRR146 pKa = 11.84 YY147 pKa = 7.51 FEE149 pKa = 4.32 RR150 pKa = 11.84 LQSEE154 pKa = 4.57 LDD156 pKa = 3.06 SGYY159 pKa = 10.62 QRR161 pKa = 11.84 GHH163 pKa = 6.19 QIAAYY168 pKa = 10.4 LFGTPGTGKK177 pKa = 9.75 SHH179 pKa = 6.88 IAHH182 pKa = 6.78 AAVEE186 pKa = 4.09 EE187 pKa = 4.26 RR188 pKa = 11.84 TWYY191 pKa = 10.97 SKK193 pKa = 11.18 DD194 pKa = 3.1 ATTKK198 pKa = 8.77 WWDD201 pKa = 3.79 GYY203 pKa = 10.84 SGEE206 pKa = 4.36 EE207 pKa = 4.13 VVIVDD212 pKa = 4.01 DD213 pKa = 3.94 WSGPSTQAEE222 pKa = 3.75 ADD224 pKa = 3.42 QWKK227 pKa = 9.48 KK228 pKa = 9.3 WVDD231 pKa = 3.54 RR232 pKa = 11.84 YY233 pKa = 9.54 PCRR236 pKa = 11.84 VEE238 pKa = 4.08 TKK240 pKa = 10.41 GGHH243 pKa = 4.53 VWLKK247 pKa = 7.19 TKK249 pKa = 9.64 QWIFTSNVRR258 pKa = 11.84 WVALPVFFDD267 pKa = 3.36 AALKK271 pKa = 10.55 RR272 pKa = 11.84 RR273 pKa = 11.84 FIGNGG278 pKa = 3.03
Molecular weight: 31.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.845
IPC2_protein 8.024
IPC_protein 7.966
Toseland 8.009
ProMoST 8.448
Dawson 8.697
Bjellqvist 8.858
Wikipedia 8.697
Rodwell 8.756
Grimsley 7.878
Solomon 8.858
Lehninger 8.843
Nozaki 9.019
DTASelect 8.668
Thurlkill 8.741
EMBOSS 8.887
Sillero 9.004
Patrickios 4.609
IPC_peptide 8.843
IPC2_peptide 7.688
IPC2.peptide.svr19 7.784
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
553
275
278
276.5
31.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.787 ± 1.353
1.447 ± 0.269
5.063 ± 0.296
5.063 ± 2.182
4.702 ± 0.846
8.68 ± 1.341
2.893 ± 0.563
5.244 ± 0.667
4.34 ± 1.634
5.425 ± 0.298
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.085 ± 0.555
3.617 ± 1.116
4.34 ± 1.395
6.329 ± 1.265
6.329 ± 1.213
6.51 ± 0.302
7.595 ± 1.408
5.787 ± 0.802
3.797 ± 0.397
5.967 ± 1.815
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here