Nocardioides sp. K1W22B-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3217 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A5HCK3|A0A3A5HCK3_9ACTN tRNA (adenine(58)-N(1))-methyltransferase TrmI OS=Nocardioides sp. K1W22B-1 OX=2321396 GN=D4739_13880 PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 5.02CVDD5 pKa = 4.12GAGQTLSLDD14 pKa = 3.44ADD16 pKa = 4.32LGYY19 pKa = 9.28STTDD23 pKa = 3.4PYY25 pKa = 11.86AVTTTFHH32 pKa = 6.35TGGGDD37 pKa = 3.98VIWTFARR44 pKa = 11.84DD45 pKa = 3.4LLIRR49 pKa = 11.84GLTSPSGDD57 pKa = 2.81GDD59 pKa = 3.9VHH61 pKa = 6.9VWPCLDD67 pKa = 3.45ASGQAVVIVEE77 pKa = 4.26LNSPDD82 pKa = 4.16GEE84 pKa = 4.34LLVQIPTKK92 pKa = 10.47DD93 pKa = 2.77VYY95 pKa = 11.14RR96 pKa = 11.84FVNRR100 pKa = 11.84TLSAVPLGSEE110 pKa = 4.49GDD112 pKa = 4.02YY113 pKa = 11.35LDD115 pKa = 5.55LDD117 pKa = 3.93ALINQLMAAA126 pKa = 4.53

Molecular weight: 13.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A5H5X1|A0A3A5H5X1_9ACTN UDP-N-acetylmuramoylalanine--D-glutamate ligase OS=Nocardioides sp. K1W22B-1 OX=2321396 GN=murD PE=3 SV=1
MM1 pKa = 7.15TPRR4 pKa = 11.84RR5 pKa = 11.84TRR7 pKa = 11.84SKK9 pKa = 10.4ASRR12 pKa = 11.84RR13 pKa = 11.84PSRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84TTGSKK23 pKa = 9.47PRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84TPRR30 pKa = 11.84LLVRR34 pKa = 11.84PTTAPCGTSRR44 pKa = 11.84PLRR47 pKa = 11.84PPRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84PRR54 pKa = 11.84PSPRR58 pKa = 11.84RR59 pKa = 11.84PSRR62 pKa = 11.84SSPATRR68 pKa = 11.84SLTPTT73 pKa = 3.41

Molecular weight: 8.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3217

0

3217

1049921

33

2989

326.4

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.025 ± 0.059

0.735 ± 0.012

6.209 ± 0.032

5.695 ± 0.044

2.991 ± 0.026

9.112 ± 0.037

2.162 ± 0.021

4.049 ± 0.025

2.475 ± 0.033

10.207 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.869 ± 0.016

2.007 ± 0.025

5.469 ± 0.032

2.766 ± 0.022

7.098 ± 0.045

5.489 ± 0.029

6.117 ± 0.046

9.064 ± 0.04

1.485 ± 0.02

1.976 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski