Streptococcus phage Javan59

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AM64|A0A4D6AM64_9CAUD Lysozyme OS=Streptococcus phage Javan59 OX=2548277 GN=Javan59_0007 PE=3 SV=1
MM1 pKa = 7.34EE2 pKa = 5.04NKK4 pKa = 9.85EE5 pKa = 3.95RR6 pKa = 11.84MQIIANNITYY16 pKa = 9.28FRR18 pKa = 11.84KK19 pKa = 9.95QKK21 pKa = 10.95GITQKK26 pKa = 10.8EE27 pKa = 3.97LAEE30 pKa = 4.72KK31 pKa = 10.39IGIQPSTLSDD41 pKa = 3.94YY42 pKa = 11.33INLRR46 pKa = 11.84SAPSFGIIQKK56 pKa = 10.25LADD59 pKa = 3.75FFEE62 pKa = 4.97INKK65 pKa = 10.02SDD67 pKa = 3.21IDD69 pKa = 3.85TTFKK73 pKa = 10.78EE74 pKa = 4.49DD75 pKa = 3.99SPPTSDD81 pKa = 2.95TARR84 pKa = 11.84AISDD88 pKa = 3.52TVEE91 pKa = 3.95KK92 pKa = 10.81LEE94 pKa = 3.88EE95 pKa = 3.9PRR97 pKa = 11.84RR98 pKa = 11.84VIVLDD103 pKa = 3.5TAKK106 pKa = 9.4TQLRR110 pKa = 11.84EE111 pKa = 3.87QEE113 pKa = 4.1RR114 pKa = 11.84ASSEE118 pKa = 3.71VHH120 pKa = 5.82EE121 pKa = 5.07PIRR124 pKa = 11.84LYY126 pKa = 11.05NYY128 pKa = 10.44DD129 pKa = 4.48YY130 pKa = 11.34YY131 pKa = 11.15DD132 pKa = 3.59QPASAGTGQYY142 pKa = 11.07LNDD145 pKa = 3.43VKK147 pKa = 11.11VEE149 pKa = 4.02QVQLPIDD156 pKa = 3.76VKK158 pKa = 11.26ADD160 pKa = 3.57FVIPIYY166 pKa = 10.25GDD168 pKa = 3.39SMEE171 pKa = 4.74PEE173 pKa = 4.48YY174 pKa = 11.08HH175 pKa = 6.24SGDD178 pKa = 3.56YY179 pKa = 11.02VFIRR183 pKa = 11.84LSVDD187 pKa = 3.55LSDD190 pKa = 4.28GDD192 pKa = 3.49IGVFEE197 pKa = 4.94YY198 pKa = 11.34YY199 pKa = 10.64GDD201 pKa = 4.19AYY203 pKa = 9.89IKK205 pKa = 10.35QLIINEE211 pKa = 4.09NGAFLHH217 pKa = 6.33SFNSDD222 pKa = 3.07YY223 pKa = 11.7DD224 pKa = 4.7DD225 pKa = 4.4IPIDD229 pKa = 3.8ADD231 pKa = 3.45SDD233 pKa = 3.65FRR235 pKa = 11.84IIGEE239 pKa = 4.24VVEE242 pKa = 4.92SFSQII247 pKa = 3.22

Molecular weight: 28.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AJZ7|A0A4D6AJZ7_9CAUD Capsid and scaffold protein OS=Streptococcus phage Javan59 OX=2548277 GN=Javan59_0026 PE=4 SV=1
MM1 pKa = 7.58EE2 pKa = 5.87LPYY5 pKa = 11.29SNAKK9 pKa = 10.43LEE11 pKa = 4.17ATNNLIKK18 pKa = 10.53VIKK21 pKa = 10.19RR22 pKa = 11.84NAFGFRR28 pKa = 11.84NFDD31 pKa = 3.48NFKK34 pKa = 10.67KK35 pKa = 10.4RR36 pKa = 11.84VLIALNIKK44 pKa = 9.9RR45 pKa = 11.84EE46 pKa = 4.07RR47 pKa = 11.84TNLVLSRR54 pKa = 11.84CC55 pKa = 3.84

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12684

43

1166

215.0

24.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.277 ± 0.343

0.536 ± 0.095

6.173 ± 0.278

7.86 ± 0.364

3.918 ± 0.239

5.937 ± 0.433

1.498 ± 0.157

6.835 ± 0.294

8.452 ± 0.313

8.641 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.16

5.527 ± 0.202

3.083 ± 0.236

4.533 ± 0.258

4.518 ± 0.281

5.944 ± 0.265

5.629 ± 0.341

6.11 ± 0.246

1.332 ± 0.101

3.997 ± 0.287
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski