Amino acid dipepetide frequency for Streptococcus phage Javan59

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.021AlaAla: 4.021 ± 0.619
0.473AlaCys: 0.473 ± 0.182
4.81AlaAsp: 4.81 ± 0.764
6.623AlaGlu: 6.623 ± 0.818
2.681AlaPhe: 2.681 ± 0.756
4.415AlaGly: 4.415 ± 0.671
1.34AlaHis: 1.34 ± 0.365
5.204AlaIle: 5.204 ± 0.607
5.677AlaLys: 5.677 ± 0.842
6.15AlaLeu: 6.15 ± 0.786
1.735AlaMet: 1.735 ± 0.379
4.258AlaAsn: 4.258 ± 0.601
2.76AlaPro: 2.76 ± 0.37
2.523AlaGln: 2.523 ± 0.511
3.39AlaArg: 3.39 ± 0.619
3.39AlaSer: 3.39 ± 0.374
3.785AlaThr: 3.785 ± 0.565
5.44AlaVal: 5.44 ± 1.162
1.183AlaTrp: 1.183 ± 0.383
2.917AlaTyr: 2.917 ± 0.398
0.0AlaXaa: 0.0 ± 0.0
Cys
0.473CysAla: 0.473 ± 0.188
0.0CysCys: 0.0 ± 0.0
0.158CysAsp: 0.158 ± 0.132
0.237CysGlu: 0.237 ± 0.139
0.079CysPhe: 0.079 ± 0.073
0.315CysGly: 0.315 ± 0.177
0.315CysHis: 0.315 ± 0.155
0.158CysIle: 0.158 ± 0.114
0.867CysLys: 0.867 ± 0.281
0.315CysLeu: 0.315 ± 0.181
0.237CysMet: 0.237 ± 0.132
0.315CysAsn: 0.315 ± 0.157
0.394CysPro: 0.394 ± 0.194
0.394CysGln: 0.394 ± 0.17
0.552CysArg: 0.552 ± 0.194
0.315CysSer: 0.315 ± 0.156
0.158CysThr: 0.158 ± 0.111
0.079CysVal: 0.079 ± 0.073
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
3.154AspAla: 3.154 ± 0.522
0.473AspCys: 0.473 ± 0.205
3.863AspAsp: 3.863 ± 0.479
4.967AspGlu: 4.967 ± 0.737
2.838AspPhe: 2.838 ± 0.397
3.469AspGly: 3.469 ± 0.484
0.473AspHis: 0.473 ± 0.187
4.258AspIle: 4.258 ± 0.568
5.677AspLys: 5.677 ± 0.689
4.967AspLeu: 4.967 ± 0.575
2.444AspMet: 2.444 ± 0.48
3.863AspAsn: 3.863 ± 0.601
2.129AspPro: 2.129 ± 0.392
1.262AspGln: 1.262 ± 0.26
2.602AspArg: 2.602 ± 0.45
3.312AspSer: 3.312 ± 0.537
3.469AspThr: 3.469 ± 0.574
3.706AspVal: 3.706 ± 0.486
1.892AspTrp: 1.892 ± 0.351
2.917AspTyr: 2.917 ± 0.683
0.0AspXaa: 0.0 ± 0.0
Glu
5.677GluAla: 5.677 ± 0.781
0.552GluCys: 0.552 ± 0.23
3.075GluAsp: 3.075 ± 0.45
4.81GluGlu: 4.81 ± 0.764
3.233GluPhe: 3.233 ± 0.612
4.1GluGly: 4.1 ± 0.636
1.34GluHis: 1.34 ± 0.245
6.544GluIle: 6.544 ± 0.657
6.229GluLys: 6.229 ± 0.747
9.698GluLeu: 9.698 ± 1.157
2.129GluMet: 2.129 ± 0.436
4.258GluAsn: 4.258 ± 0.623
1.735GluPro: 1.735 ± 0.433
4.888GluGln: 4.888 ± 0.764
4.1GluArg: 4.1 ± 0.642
3.627GluSer: 3.627 ± 0.422
3.154GluThr: 3.154 ± 0.484
5.362GluVal: 5.362 ± 0.903
1.025GluTrp: 1.025 ± 0.197
3.075GluTyr: 3.075 ± 0.542
0.0GluXaa: 0.0 ± 0.0
Phe
2.129PheAla: 2.129 ± 0.42
0.237PheCys: 0.237 ± 0.167
3.627PheAsp: 3.627 ± 0.516
3.785PheGlu: 3.785 ± 0.488
1.183PhePhe: 1.183 ± 0.276
2.365PheGly: 2.365 ± 0.482
0.473PheHis: 0.473 ± 0.225
1.971PheIle: 1.971 ± 0.426
3.706PheLys: 3.706 ± 0.784
2.996PheLeu: 2.996 ± 0.493
1.104PheMet: 1.104 ± 0.275
2.602PheAsn: 2.602 ± 0.455
0.946PhePro: 0.946 ± 0.293
0.946PheGln: 0.946 ± 0.221
2.287PheArg: 2.287 ± 0.423
2.996PheSer: 2.996 ± 0.72
2.208PheThr: 2.208 ± 0.525
2.129PheVal: 2.129 ± 0.335
0.394PheTrp: 0.394 ± 0.173
1.104PheTyr: 1.104 ± 0.3
0.0PheXaa: 0.0 ± 0.0
Gly
3.627GlyAla: 3.627 ± 0.665
0.315GlyCys: 0.315 ± 0.127
3.39GlyAsp: 3.39 ± 0.721
3.942GlyGlu: 3.942 ± 0.562
2.444GlyPhe: 2.444 ± 0.534
3.942GlyGly: 3.942 ± 0.75
0.867GlyHis: 0.867 ± 0.228
5.046GlyIle: 5.046 ± 0.813
5.362GlyLys: 5.362 ± 0.512
5.125GlyLeu: 5.125 ± 0.848
1.656GlyMet: 1.656 ± 0.344
2.838GlyAsn: 2.838 ± 0.539
0.788GlyPro: 0.788 ± 0.227
2.76GlyGln: 2.76 ± 0.61
2.996GlyArg: 2.996 ± 0.406
3.312GlySer: 3.312 ± 0.516
3.154GlyThr: 3.154 ± 0.562
4.1GlyVal: 4.1 ± 0.69
1.025GlyTrp: 1.025 ± 0.304
2.681GlyTyr: 2.681 ± 0.362
0.0GlyXaa: 0.0 ± 0.0
His
1.34HisAla: 1.34 ± 0.344
0.0HisCys: 0.0 ± 0.0
0.71HisAsp: 0.71 ± 0.236
1.183HisGlu: 1.183 ± 0.265
0.946HisPhe: 0.946 ± 0.268
0.946HisGly: 0.946 ± 0.235
0.315HisHis: 0.315 ± 0.163
0.788HisIle: 0.788 ± 0.235
1.498HisLys: 1.498 ± 0.371
1.577HisLeu: 1.577 ± 0.391
0.237HisMet: 0.237 ± 0.124
0.71HisAsn: 0.71 ± 0.218
0.552HisPro: 0.552 ± 0.189
0.394HisGln: 0.394 ± 0.163
0.552HisArg: 0.552 ± 0.204
1.025HisSer: 1.025 ± 0.274
0.867HisThr: 0.867 ± 0.254
0.788HisVal: 0.788 ± 0.297
0.237HisTrp: 0.237 ± 0.129
0.315HisTyr: 0.315 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
5.756IleAla: 5.756 ± 0.754
0.473IleCys: 0.473 ± 0.161
4.888IleAsp: 4.888 ± 0.606
5.992IleGlu: 5.992 ± 0.638
2.76IlePhe: 2.76 ± 0.587
3.942IleGly: 3.942 ± 0.935
0.788IleHis: 0.788 ± 0.266
4.258IleIle: 4.258 ± 0.604
6.623IleLys: 6.623 ± 0.863
4.337IleLeu: 4.337 ± 0.722
0.946IleMet: 0.946 ± 0.351
3.942IleAsn: 3.942 ± 0.558
2.287IlePro: 2.287 ± 0.416
2.365IleGln: 2.365 ± 0.466
2.917IleArg: 2.917 ± 0.527
4.021IleSer: 4.021 ± 0.704
4.337IleThr: 4.337 ± 0.909
3.863IleVal: 3.863 ± 0.514
0.631IleTrp: 0.631 ± 0.151
3.233IleTyr: 3.233 ± 0.572
0.0IleXaa: 0.0 ± 0.0
Lys
5.756LysAla: 5.756 ± 0.657
0.079LysCys: 0.079 ± 0.092
4.573LysAsp: 4.573 ± 0.573
7.411LysGlu: 7.411 ± 0.754
3.39LysPhe: 3.39 ± 0.473
4.652LysGly: 4.652 ± 0.45
0.946LysHis: 0.946 ± 0.282
4.731LysIle: 4.731 ± 0.705
8.831LysLys: 8.831 ± 0.936
7.569LysLeu: 7.569 ± 0.67
1.971LysMet: 1.971 ± 0.391
3.785LysAsn: 3.785 ± 0.486
1.892LysPro: 1.892 ± 0.37
4.573LysGln: 4.573 ± 0.843
4.258LysArg: 4.258 ± 0.605
5.519LysSer: 5.519 ± 0.61
5.835LysThr: 5.835 ± 0.684
5.125LysVal: 5.125 ± 0.811
0.946LysTrp: 0.946 ± 0.271
2.602LysTyr: 2.602 ± 0.477
0.0LysXaa: 0.0 ± 0.0
Leu
8.279LeuAla: 8.279 ± 0.76
0.552LeuCys: 0.552 ± 0.214
6.387LeuAsp: 6.387 ± 0.905
8.515LeuGlu: 8.515 ± 0.695
3.154LeuPhe: 3.154 ± 0.629
5.125LeuGly: 5.125 ± 0.983
1.577LeuHis: 1.577 ± 0.346
4.731LeuIle: 4.731 ± 0.626
7.411LeuLys: 7.411 ± 0.74
6.938LeuLeu: 6.938 ± 0.963
1.419LeuMet: 1.419 ± 0.306
4.888LeuAsn: 4.888 ± 0.503
3.39LeuPro: 3.39 ± 0.602
3.548LeuGln: 3.548 ± 0.463
4.1LeuArg: 4.1 ± 0.597
5.44LeuSer: 5.44 ± 0.933
4.1LeuThr: 4.1 ± 0.489
4.573LeuVal: 4.573 ± 0.659
0.631LeuTrp: 0.631 ± 0.169
1.656LeuTyr: 1.656 ± 0.38
0.0LeuXaa: 0.0 ± 0.0
Met
1.735MetAla: 1.735 ± 0.398
0.0MetCys: 0.0 ± 0.0
1.183MetAsp: 1.183 ± 0.333
1.892MetGlu: 1.892 ± 0.364
0.394MetPhe: 0.394 ± 0.141
1.025MetGly: 1.025 ± 0.262
0.158MetHis: 0.158 ± 0.12
1.104MetIle: 1.104 ± 0.351
1.577MetLys: 1.577 ± 0.352
2.208MetLeu: 2.208 ± 0.349
0.079MetMet: 0.079 ± 0.077
1.34MetAsn: 1.34 ± 0.328
0.788MetPro: 0.788 ± 0.253
1.183MetGln: 1.183 ± 0.298
1.498MetArg: 1.498 ± 0.375
1.656MetSer: 1.656 ± 0.351
2.05MetThr: 2.05 ± 0.32
0.71MetVal: 0.71 ± 0.213
0.394MetTrp: 0.394 ± 0.166
1.025MetTyr: 1.025 ± 0.252
0.0MetXaa: 0.0 ± 0.0
Asn
5.362AsnAla: 5.362 ± 1.09
0.079AsnCys: 0.079 ± 0.075
3.233AsnAsp: 3.233 ± 0.512
3.39AsnGlu: 3.39 ± 0.619
1.656AsnPhe: 1.656 ± 0.371
4.258AsnGly: 4.258 ± 0.479
0.788AsnHis: 0.788 ± 0.248
4.415AsnIle: 4.415 ± 0.63
4.179AsnLys: 4.179 ± 0.556
4.494AsnLeu: 4.494 ± 0.603
0.946AsnMet: 0.946 ± 0.246
3.312AsnAsn: 3.312 ± 0.436
1.892AsnPro: 1.892 ± 0.36
2.05AsnGln: 2.05 ± 0.463
2.208AsnArg: 2.208 ± 0.36
3.469AsnSer: 3.469 ± 0.405
2.681AsnThr: 2.681 ± 0.421
2.76AsnVal: 2.76 ± 0.453
1.025AsnTrp: 1.025 ± 0.245
3.075AsnTyr: 3.075 ± 0.575
0.0AsnXaa: 0.0 ± 0.0
Pro
1.735ProAla: 1.735 ± 0.34
0.079ProCys: 0.079 ± 0.071
2.05ProAsp: 2.05 ± 0.426
3.154ProGlu: 3.154 ± 0.463
1.025ProPhe: 1.025 ± 0.271
1.183ProGly: 1.183 ± 0.285
0.237ProHis: 0.237 ± 0.141
2.444ProIle: 2.444 ± 0.571
2.681ProLys: 2.681 ± 0.461
2.523ProLeu: 2.523 ± 0.44
0.315ProMet: 0.315 ± 0.126
1.735ProAsn: 1.735 ± 0.395
0.867ProPro: 0.867 ± 0.242
1.735ProGln: 1.735 ± 0.465
0.946ProArg: 0.946 ± 0.276
2.05ProSer: 2.05 ± 0.496
1.735ProThr: 1.735 ± 0.372
2.05ProVal: 2.05 ± 0.492
0.315ProTrp: 0.315 ± 0.135
1.971ProTyr: 1.971 ± 0.368
0.0ProXaa: 0.0 ± 0.0
Gln
3.706GlnAla: 3.706 ± 0.512
0.315GlnCys: 0.315 ± 0.185
1.419GlnAsp: 1.419 ± 0.385
3.154GlnGlu: 3.154 ± 0.662
1.735GlnPhe: 1.735 ± 0.417
2.523GlnGly: 2.523 ± 0.449
0.631GlnHis: 0.631 ± 0.192
3.312GlnIle: 3.312 ± 0.503
3.942GlnLys: 3.942 ± 0.478
3.706GlnLeu: 3.706 ± 0.513
1.419GlnMet: 1.419 ± 0.356
1.813GlnAsn: 1.813 ± 0.363
1.735GlnPro: 1.735 ± 0.402
3.39GlnGln: 3.39 ± 0.704
2.05GlnArg: 2.05 ± 0.367
2.917GlnSer: 2.917 ± 0.484
2.287GlnThr: 2.287 ± 0.568
2.996GlnVal: 2.996 ± 0.521
0.631GlnTrp: 0.631 ± 0.198
1.656GlnTyr: 1.656 ± 0.339
0.0GlnXaa: 0.0 ± 0.0
Arg
3.469ArgAla: 3.469 ± 0.412
0.237ArgCys: 0.237 ± 0.141
2.208ArgAsp: 2.208 ± 0.465
3.627ArgGlu: 3.627 ± 0.623
1.656ArgPhe: 1.656 ± 0.362
2.365ArgGly: 2.365 ± 0.436
1.025ArgHis: 1.025 ± 0.312
2.996ArgIle: 2.996 ± 0.525
4.258ArgLys: 4.258 ± 0.629
4.81ArgLeu: 4.81 ± 0.797
1.183ArgMet: 1.183 ± 0.291
2.208ArgAsn: 2.208 ± 0.409
0.788ArgPro: 0.788 ± 0.28
1.498ArgGln: 1.498 ± 0.256
1.971ArgArg: 1.971 ± 0.433
1.656ArgSer: 1.656 ± 0.388
2.76ArgThr: 2.76 ± 0.434
3.706ArgVal: 3.706 ± 0.605
0.473ArgTrp: 0.473 ± 0.206
2.287ArgTyr: 2.287 ± 0.599
0.0ArgXaa: 0.0 ± 0.0
Ser
4.415SerAla: 4.415 ± 0.669
0.079SerCys: 0.079 ± 0.089
4.021SerAsp: 4.021 ± 0.812
3.942SerGlu: 3.942 ± 0.759
2.602SerPhe: 2.602 ± 0.478
4.179SerGly: 4.179 ± 0.619
0.867SerHis: 0.867 ± 0.265
4.652SerIle: 4.652 ± 0.544
3.627SerLys: 3.627 ± 0.449
5.046SerLeu: 5.046 ± 0.561
1.262SerMet: 1.262 ± 0.26
3.39SerAsn: 3.39 ± 0.464
1.971SerPro: 1.971 ± 0.291
2.76SerGln: 2.76 ± 0.445
2.05SerArg: 2.05 ± 0.427
3.075SerSer: 3.075 ± 0.533
4.1SerThr: 4.1 ± 0.448
3.233SerVal: 3.233 ± 0.598
1.104SerTrp: 1.104 ± 0.254
3.075SerTyr: 3.075 ± 0.578
0.0SerXaa: 0.0 ± 0.0
Thr
4.415ThrAla: 4.415 ± 0.604
0.315ThrCys: 0.315 ± 0.162
3.627ThrAsp: 3.627 ± 0.492
4.1ThrGlu: 4.1 ± 0.7
2.523ThrPhe: 2.523 ± 0.465
3.785ThrGly: 3.785 ± 0.634
1.183ThrHis: 1.183 ± 0.359
4.1ThrIle: 4.1 ± 0.569
3.312ThrLys: 3.312 ± 0.618
4.258ThrLeu: 4.258 ± 0.611
1.183ThrMet: 1.183 ± 0.339
2.76ThrAsn: 2.76 ± 0.461
2.208ThrPro: 2.208 ± 0.532
3.469ThrGln: 3.469 ± 0.489
1.735ThrArg: 1.735 ± 0.325
3.548ThrSer: 3.548 ± 0.597
3.548ThrThr: 3.548 ± 0.632
3.627ThrVal: 3.627 ± 0.486
0.158ThrTrp: 0.158 ± 0.109
2.444ThrTyr: 2.444 ± 0.41
0.0ThrXaa: 0.0 ± 0.0
Val
4.652ValAla: 4.652 ± 0.658
0.552ValCys: 0.552 ± 0.211
4.179ValAsp: 4.179 ± 0.581
4.415ValGlu: 4.415 ± 0.715
2.365ValPhe: 2.365 ± 0.411
4.179ValGly: 4.179 ± 0.657
0.788ValHis: 0.788 ± 0.231
3.863ValIle: 3.863 ± 0.623
4.573ValLys: 4.573 ± 0.651
6.387ValLeu: 6.387 ± 0.738
0.946ValMet: 0.946 ± 0.34
3.233ValAsn: 3.233 ± 0.564
1.735ValPro: 1.735 ± 0.304
2.208ValGln: 2.208 ± 0.373
2.287ValArg: 2.287 ± 0.392
4.81ValSer: 4.81 ± 0.447
3.154ValThr: 3.154 ± 0.485
4.021ValVal: 4.021 ± 0.616
0.552ValTrp: 0.552 ± 0.162
2.208ValTyr: 2.208 ± 0.416
0.0ValXaa: 0.0 ± 0.0
Trp
0.788TrpAla: 0.788 ± 0.224
0.158TrpCys: 0.158 ± 0.109
1.34TrpAsp: 1.34 ± 0.357
1.104TrpGlu: 1.104 ± 0.27
1.104TrpPhe: 1.104 ± 0.566
0.394TrpGly: 0.394 ± 0.155
0.158TrpHis: 0.158 ± 0.095
0.946TrpIle: 0.946 ± 0.291
1.104TrpLys: 1.104 ± 0.336
0.71TrpLeu: 0.71 ± 0.252
0.237TrpMet: 0.237 ± 0.138
0.946TrpAsn: 0.946 ± 0.254
0.158TrpPro: 0.158 ± 0.113
0.71TrpGln: 0.71 ± 0.182
0.473TrpArg: 0.473 ± 0.203
0.946TrpSer: 0.946 ± 0.228
0.631TrpThr: 0.631 ± 0.215
0.946TrpVal: 0.946 ± 0.283
0.079TrpTrp: 0.079 ± 0.08
0.394TrpTyr: 0.394 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.287TyrAla: 2.287 ± 0.462
0.394TyrCys: 0.394 ± 0.167
2.996TyrAsp: 2.996 ± 0.508
2.365TyrGlu: 2.365 ± 0.523
1.419TyrPhe: 1.419 ± 0.314
2.208TyrGly: 2.208 ± 0.551
0.71TyrHis: 0.71 ± 0.233
2.838TyrIle: 2.838 ± 0.407
3.39TyrLys: 3.39 ± 0.63
2.602TyrLeu: 2.602 ± 0.401
0.473TyrMet: 0.473 ± 0.189
3.075TyrAsn: 3.075 ± 0.49
1.813TyrPro: 1.813 ± 0.338
2.681TyrGln: 2.681 ± 0.619
2.208TyrArg: 2.208 ± 0.407
2.365TyrSer: 2.365 ± 0.399
2.287TyrThr: 2.287 ± 0.423
1.892TyrVal: 1.892 ± 0.386
0.631TyrTrp: 0.631 ± 0.236
1.34TyrTyr: 1.34 ± 0.385
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (12684 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski