Mesorhizobium sp. J18

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; unclassified Mesorhizobium

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4171 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562C1S7|A0A562C1S7_9RHIZ TetR family transcriptional regulator OS=Mesorhizobium sp. J18 OX=935263 GN=L598_000600001070 PE=4 SV=1
MM1 pKa = 7.03KK2 pKa = 9.7TVANARR8 pKa = 11.84GVNLYY13 pKa = 10.92YY14 pKa = 10.57SDD16 pKa = 3.34TSINHH21 pKa = 6.06FSATNSGPQLYY32 pKa = 7.54GTPDD36 pKa = 3.61NDD38 pKa = 4.44SLWGDD43 pKa = 3.62SSVDD47 pKa = 3.09VTMYY51 pKa = 10.91GGAGDD56 pKa = 5.07DD57 pKa = 3.1IYY59 pKa = 11.79YY60 pKa = 10.33LYY62 pKa = 11.2SPINRR67 pKa = 11.84AAEE70 pKa = 4.16DD71 pKa = 3.7PGEE74 pKa = 4.3GVDD77 pKa = 5.57TIVTWMSTRR86 pKa = 11.84LPDD89 pKa = 3.46NFEE92 pKa = 4.19NLTVTGSGRR101 pKa = 11.84YY102 pKa = 9.45AFGNAADD109 pKa = 4.48NIIKK113 pKa = 10.38GGSGQQTLDD122 pKa = 3.43GGSGNDD128 pKa = 3.37VLIGGGGADD137 pKa = 2.89IFVVSKK143 pKa = 11.4GNGSDD148 pKa = 4.54LIFDD152 pKa = 4.63FEE154 pKa = 4.39TTDD157 pKa = 3.22RR158 pKa = 11.84VRR160 pKa = 11.84LDD162 pKa = 3.47GYY164 pKa = 10.45GWNSFDD170 pKa = 4.33AVQDD174 pKa = 3.08RR175 pKa = 11.84MTQVGSDD182 pKa = 3.19VRR184 pKa = 11.84LDD186 pKa = 3.7LGVGEE191 pKa = 4.43ILVFKK196 pKa = 9.84DD197 pKa = 3.11TTIDD201 pKa = 3.54QFQADD206 pKa = 4.31QFEE209 pKa = 4.68LPLDD213 pKa = 4.11RR214 pKa = 11.84SAMSLSFAEE223 pKa = 4.46HH224 pKa = 6.76FNTLSLWDD232 pKa = 4.08GEE234 pKa = 4.41SGRR237 pKa = 11.84WDD239 pKa = 3.69SNFWWGAPNGSTLSGNSEE257 pKa = 4.2LQWYY261 pKa = 9.34IDD263 pKa = 3.61ANYY266 pKa = 10.53GPTSSVNPFSVEE278 pKa = 3.87NGVLTITAAPTPDD291 pKa = 3.9EE292 pKa = 4.61IKK294 pKa = 10.73PLINNYY300 pKa = 10.02EE301 pKa = 4.22YY302 pKa = 10.86TSGLLTTYY310 pKa = 10.59HH311 pKa = 6.53SFSQTYY317 pKa = 10.0GYY319 pKa = 11.18FEE321 pKa = 4.44MRR323 pKa = 11.84ADD325 pKa = 3.45MPEE328 pKa = 3.94NQGAWPAFWLLPADD342 pKa = 4.39GSWPPEE348 pKa = 3.55IDD350 pKa = 3.58VVEE353 pKa = 4.39MRR355 pKa = 11.84GQDD358 pKa = 3.36PNTIHH363 pKa = 5.66MTAHH367 pKa = 5.54TNEE370 pKa = 4.58TGSHH374 pKa = 4.81TMVSSAINVSDD385 pKa = 3.48TEE387 pKa = 4.23GFHH390 pKa = 6.52TYY392 pKa = 9.82GVLWTEE398 pKa = 5.5DD399 pKa = 3.37EE400 pKa = 4.44LVWYY404 pKa = 10.09FDD406 pKa = 3.89DD407 pKa = 3.91VAVARR412 pKa = 11.84AEE414 pKa = 4.49TPSDD418 pKa = 3.33MHH420 pKa = 8.33DD421 pKa = 3.43PMYY424 pKa = 10.44MLVNLAIGGPAGEE437 pKa = 5.01PDD439 pKa = 4.54DD440 pKa = 4.92GLAVPAEE447 pKa = 3.99MQIDD451 pKa = 4.22YY452 pKa = 10.79IRR454 pKa = 11.84AYY456 pKa = 9.92EE457 pKa = 4.08LDD459 pKa = 3.79EE460 pKa = 4.33VPEE463 pKa = 4.14VSLLANLFDD472 pKa = 4.02TLDD475 pKa = 3.37WLII478 pKa = 4.2

Molecular weight: 52.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562CPY8|A0A562CPY8_9RHIZ AbrB family looped-hinge helix DNA binding protein OS=Mesorhizobium sp. J18 OX=935263 GN=L598_001500000800 PE=4 SV=1
MM1 pKa = 6.42TAGMKK6 pKa = 10.29RR7 pKa = 11.84EE8 pKa = 4.12DD9 pKa = 3.36RR10 pKa = 11.84SSRR13 pKa = 11.84EE14 pKa = 3.59KK15 pKa = 10.16MRR17 pKa = 11.84VSRR20 pKa = 11.84FLAAGPATIAVAGSGDD36 pKa = 3.67NLLLEE41 pKa = 4.44TEE43 pKa = 4.12NRR45 pKa = 11.84GTISVRR51 pKa = 11.84RR52 pKa = 11.84GEE54 pKa = 4.12FRR56 pKa = 11.84ALLAQGLVVVSGKK69 pKa = 9.12CARR72 pKa = 11.84LSAEE76 pKa = 3.91GQALARR82 pKa = 11.84RR83 pKa = 11.84AAATVEE89 pKa = 4.21PFRR92 pKa = 11.84AQHH95 pKa = 6.34GEE97 pKa = 3.93TGSVVMEE104 pKa = 3.94VQGMRR109 pKa = 11.84EE110 pKa = 3.83TVEE113 pKa = 3.92INLAEE118 pKa = 4.34SPLAQLASRR127 pKa = 11.84RR128 pKa = 11.84TRR130 pKa = 11.84KK131 pKa = 10.18GEE133 pKa = 3.83RR134 pKa = 11.84FLTGAEE140 pKa = 3.5IRR142 pKa = 11.84AGEE145 pKa = 4.41RR146 pKa = 11.84LRR148 pKa = 11.84ADD150 pKa = 3.46YY151 pKa = 10.1TRR153 pKa = 11.84GQIMPRR159 pKa = 11.84ITANWQAAVASGRR172 pKa = 11.84RR173 pKa = 11.84AGGSGGMVEE182 pKa = 4.5LTDD185 pKa = 3.45AALAARR191 pKa = 11.84QRR193 pKa = 11.84VDD195 pKa = 2.92RR196 pKa = 11.84ALQAVGPEE204 pKa = 4.07LAGVLVDD211 pKa = 3.39VCCFLKK217 pKa = 10.77GLEE220 pKa = 4.07RR221 pKa = 11.84VEE223 pKa = 4.49TEE225 pKa = 3.63RR226 pKa = 11.84GWPVRR231 pKa = 11.84SAKK234 pKa = 10.22VVLKK238 pKa = 9.34TALGALSRR246 pKa = 11.84HH247 pKa = 5.43YY248 pKa = 10.26EE249 pKa = 3.73PHH251 pKa = 6.53LCSGSQSRR259 pKa = 11.84PILHH263 pKa = 6.97WGAEE267 pKa = 4.56GYY269 pKa = 9.82RR270 pKa = 11.84PKK272 pKa = 10.84IMGG275 pKa = 3.69

Molecular weight: 29.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4171

0

4171

1255211

22

2297

300.9

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.801 ± 0.045

0.811 ± 0.013

5.556 ± 0.034

6.456 ± 0.039

3.874 ± 0.026

8.604 ± 0.041

2.011 ± 0.02

5.629 ± 0.028

3.433 ± 0.029

9.774 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.616 ± 0.017

2.657 ± 0.021

5.041 ± 0.026

2.935 ± 0.021

7.253 ± 0.036

5.459 ± 0.027

5.135 ± 0.026

7.339 ± 0.03

1.294 ± 0.015

2.307 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski