Amino acid dipepetide frequency for Mesorhizobium sp. J18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.511AlaAla: 15.511 ± 0.169	0.975AlaCys: 0.975 ± 0.029	6.353AlaAsp: 6.353 ± 0.074	8.325AlaGlu: 8.325 ± 0.095	4.53AlaPhe: 4.53 ± 0.066	10.684AlaGly: 10.684 ± 0.114	2.039AlaHis: 2.039 ± 0.039	6.769AlaIle: 6.769 ± 0.093	3.915AlaLys: 3.915 ± 0.072	12.121AlaLeu: 12.121 ± 0.107	3.437AlaMet: 3.437 ± 0.056	2.852AlaAsn: 2.852 ± 0.048	4.73AlaPro: 4.73 ± 0.068	3.313AlaGln: 3.313 ± 0.053	8.331AlaArg: 8.331 ± 0.084	6.086AlaSer: 6.086 ± 0.07	5.441AlaThr: 5.441 ± 0.08	8.481AlaVal: 8.481 ± 0.09	1.409AlaTrp: 1.409 ± 0.037	2.686AlaTyr: 2.686 ± 0.046	0.022AlaXaa: 0.022 ± 0.004
Cys	0.826CysAla: 0.826 ± 0.029	0.09CysCys: 0.09 ± 0.01	0.513CysAsp: 0.513 ± 0.021	0.466CysGlu: 0.466 ± 0.02	0.343CysPhe: 0.343 ± 0.017	0.885CysGly: 0.885 ± 0.034	0.22CysHis: 0.22 ± 0.012	0.376CysIle: 0.376 ± 0.018	0.194CysLys: 0.194 ± 0.014	0.796CysLeu: 0.796 ± 0.026	0.185CysMet: 0.185 ± 0.013	0.185CysAsn: 0.185 ± 0.013	0.39CysPro: 0.39 ± 0.019	0.214CysGln: 0.214 ± 0.013	0.668CysArg: 0.668 ± 0.024	0.489CysSer: 0.489 ± 0.019	0.355CysThr: 0.355 ± 0.019	0.58CysVal: 0.58 ± 0.024	0.117CysTrp: 0.117 ± 0.009	0.216CysTyr: 0.216 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.122AspAla: 6.122 ± 0.073	0.484AspCys: 0.484 ± 0.021	2.918AspAsp: 2.918 ± 0.053	3.81AspGlu: 3.81 ± 0.064	2.265AspPhe: 2.265 ± 0.048	4.936AspGly: 4.936 ± 0.076	1.222AspHis: 1.222 ± 0.031	3.311AspIle: 3.311 ± 0.053	1.634AspLys: 1.634 ± 0.042	5.578AspLeu: 5.578 ± 0.076	1.431AspMet: 1.431 ± 0.035	1.369AspAsn: 1.369 ± 0.036	3.384AspPro: 3.384 ± 0.054	1.577AspGln: 1.577 ± 0.039	4.557AspArg: 4.557 ± 0.066	2.173AspSer: 2.173 ± 0.045	2.495AspThr: 2.495 ± 0.044	3.851AspVal: 3.851 ± 0.062	0.903AspTrp: 0.903 ± 0.026	1.531AspTyr: 1.531 ± 0.035	0.003AspXaa: 0.003 ± 0.002
Glu	8.252GluAla: 8.252 ± 0.082	0.402GluCys: 0.402 ± 0.017	3.149GluAsp: 3.149 ± 0.061	4.406GluGlu: 4.406 ± 0.081	2.012GluPhe: 2.012 ± 0.039	4.908GluGly: 4.908 ± 0.066	1.287GluHis: 1.287 ± 0.036	4.183GluIle: 4.183 ± 0.059	3.069GluLys: 3.069 ± 0.057	5.675GluLeu: 5.675 ± 0.073	1.739GluMet: 1.739 ± 0.039	2.01GluAsn: 2.01 ± 0.04	3.05GluPro: 3.05 ± 0.053	2.224GluGln: 2.224 ± 0.045	5.496GluArg: 5.496 ± 0.081	2.679GluSer: 2.679 ± 0.045	3.904GluThr: 3.904 ± 0.062	4.167GluVal: 4.167 ± 0.065	0.788GluTrp: 0.788 ± 0.026	1.151GluTyr: 1.151 ± 0.036	0.005GluXaa: 0.005 ± 0.002
Phe	4.55PheAla: 4.55 ± 0.063	0.385PheCys: 0.385 ± 0.017	2.666PheAsp: 2.666 ± 0.049	2.432PheGlu: 2.432 ± 0.051	1.555PhePhe: 1.555 ± 0.038	3.829PheGly: 3.829 ± 0.054	0.825PheHis: 0.825 ± 0.028	1.913PheIle: 1.913 ± 0.034	0.952PheLys: 0.952 ± 0.025	3.662PheLeu: 3.662 ± 0.061	0.94PheMet: 0.94 ± 0.026	1.048PheAsn: 1.048 ± 0.029	1.661PhePro: 1.661 ± 0.037	1.04PheGln: 1.04 ± 0.029	2.545PheArg: 2.545 ± 0.047	2.35PheSer: 2.35 ± 0.042	1.937PheThr: 1.937 ± 0.041	2.951PheVal: 2.951 ± 0.059	0.553PheTrp: 0.553 ± 0.023	0.95PheTyr: 0.95 ± 0.027	0.002PheXaa: 0.002 ± 0.002
Gly	8.578GlyAla: 8.578 ± 0.098	0.827GlyCys: 0.827 ± 0.028	4.291GlyAsp: 4.291 ± 0.062	5.368GlyGlu: 5.368 ± 0.063	3.764GlyPhe: 3.764 ± 0.068	7.285GlyGly: 7.285 ± 0.101	1.863GlyHis: 1.863 ± 0.039	5.291GlyIle: 5.291 ± 0.07	3.5GlyLys: 3.5 ± 0.071	8.607GlyLeu: 8.607 ± 0.081	2.558GlyMet: 2.558 ± 0.043	2.286GlyAsn: 2.286 ± 0.05	3.471GlyPro: 3.471 ± 0.053	2.725GlyGln: 2.725 ± 0.048	6.424GlyArg: 6.424 ± 0.07	4.925GlySer: 4.925 ± 0.082	4.704GlyThr: 4.704 ± 0.08	5.923GlyVal: 5.923 ± 0.059	1.284GlyTrp: 1.284 ± 0.034	2.355GlyTyr: 2.355 ± 0.047	0.015GlyXaa: 0.015 ± 0.003
His	2.188HisAla: 2.188 ± 0.044	0.233HisCys: 0.233 ± 0.013	1.219HisAsp: 1.219 ± 0.039	1.146HisGlu: 1.146 ± 0.032	0.855HisPhe: 0.855 ± 0.029	1.892HisGly: 1.892 ± 0.04	0.539HisHis: 0.539 ± 0.025	1.025HisIle: 1.025 ± 0.029	0.527HisLys: 0.527 ± 0.02	1.948HisLeu: 1.948 ± 0.042	0.489HisMet: 0.489 ± 0.02	0.439HisAsn: 0.439 ± 0.02	1.27HisPro: 1.27 ± 0.036	0.588HisGln: 0.588 ± 0.019	1.441HisArg: 1.441 ± 0.036	1.02HisSer: 1.02 ± 0.03	0.841HisThr: 0.841 ± 0.024	1.561HisVal: 1.561 ± 0.036	0.308HisTrp: 0.308 ± 0.017	0.58HisTyr: 0.58 ± 0.025	0.001HisXaa: 0.001 ± 0.001
Ile	7.567IleAla: 7.567 ± 0.088	0.56IleCys: 0.56 ± 0.021	3.685IleAsp: 3.685 ± 0.051	4.0IleGlu: 4.0 ± 0.059	2.0IlePhe: 2.0 ± 0.046	5.444IleGly: 5.444 ± 0.074	1.012IleHis: 1.012 ± 0.025	2.734IleIle: 2.734 ± 0.058	1.384IleLys: 1.384 ± 0.033	5.009IleLeu: 5.009 ± 0.071	1.187IleMet: 1.187 ± 0.03	1.464IleAsn: 1.464 ± 0.034	2.556IlePro: 2.556 ± 0.047	1.246IleGln: 1.246 ± 0.028	3.832IleArg: 3.832 ± 0.059	3.274IleSer: 3.274 ± 0.056	2.811IleThr: 2.811 ± 0.046	4.64IleVal: 4.64 ± 0.075	0.653IleTrp: 0.653 ± 0.025	1.232IleTyr: 1.232 ± 0.029	0.002IleXaa: 0.002 ± 0.001
Lys	4.502LysAla: 4.502 ± 0.064	0.164LysCys: 0.164 ± 0.012	1.78LysAsp: 1.78 ± 0.042	2.052LysGlu: 2.052 ± 0.04	0.936LysPhe: 0.936 ± 0.024	2.781LysGly: 2.781 ± 0.054	0.605LysHis: 0.605 ± 0.019	1.849LysIle: 1.849 ± 0.047	1.498LysLys: 1.498 ± 0.039	3.329LysLeu: 3.329 ± 0.052	0.798LysMet: 0.798 ± 0.029	0.884LysAsn: 0.884 ± 0.027	2.184LysPro: 2.184 ± 0.04	1.051LysGln: 1.051 ± 0.031	2.533LysArg: 2.533 ± 0.044	1.827LysSer: 1.827 ± 0.04	2.005LysThr: 2.005 ± 0.039	2.526LysVal: 2.526 ± 0.047	0.376LysTrp: 0.376 ± 0.015	0.648LysTyr: 0.648 ± 0.023	0.001LysXaa: 0.001 ± 0.001
Leu	12.629LeuAla: 12.629 ± 0.127	0.821LeuCys: 0.821 ± 0.024	5.754LeuAsp: 5.754 ± 0.072	5.727LeuGlu: 5.727 ± 0.069	3.799LeuPhe: 3.799 ± 0.063	8.008LeuGly: 8.008 ± 0.089	1.79LeuHis: 1.79 ± 0.04	5.139LeuIle: 5.139 ± 0.075	3.723LeuLys: 3.723 ± 0.054	9.199LeuLeu: 9.199 ± 0.113	2.343LeuMet: 2.343 ± 0.042	2.471LeuAsn: 2.471 ± 0.046	5.311LeuPro: 5.311 ± 0.073	2.617LeuGln: 2.617 ± 0.046	6.395LeuArg: 6.395 ± 0.086	6.205LeuSer: 6.205 ± 0.072	5.096LeuThr: 5.096 ± 0.07	7.539LeuVal: 7.539 ± 0.089	1.085LeuTrp: 1.085 ± 0.032	2.081LeuTyr: 2.081 ± 0.041	0.012LeuXaa: 0.012 ± 0.003
Met	3.274MetAla: 3.274 ± 0.052	0.151MetCys: 0.151 ± 0.009	1.162MetAsp: 1.162 ± 0.034	1.413MetGlu: 1.413 ± 0.032	0.748MetPhe: 0.748 ± 0.022	1.924MetGly: 1.924 ± 0.042	0.442MetHis: 0.442 ± 0.019	1.507MetIle: 1.507 ± 0.036	1.093MetLys: 1.093 ± 0.03	2.58MetLeu: 2.58 ± 0.051	0.714MetMet: 0.714 ± 0.023	0.843MetAsn: 0.843 ± 0.028	1.579MetPro: 1.579 ± 0.036	0.856MetGln: 0.856 ± 0.026	2.047MetArg: 2.047 ± 0.044	1.635MetSer: 1.635 ± 0.038	1.82MetThr: 1.82 ± 0.036	1.836MetVal: 1.836 ± 0.042	0.233MetTrp: 0.233 ± 0.013	0.304MetTyr: 0.304 ± 0.014	0.002MetXaa: 0.002 ± 0.001
Asn	3.156AsnAla: 3.156 ± 0.05	0.226AsnCys: 0.226 ± 0.017	1.377AsnAsp: 1.377 ± 0.033	1.452AsnGlu: 1.452 ± 0.039	0.97AsnPhe: 0.97 ± 0.029	2.536AsnGly: 2.536 ± 0.05	0.523AsnHis: 0.523 ± 0.02	1.497AsnIle: 1.497 ± 0.031	0.687AsnLys: 0.687 ± 0.025	2.564AsnLeu: 2.564 ± 0.047	0.662AsnMet: 0.662 ± 0.026	0.717AsnAsn: 0.717 ± 0.023	1.956AsnPro: 1.956 ± 0.045	0.739AsnGln: 0.739 ± 0.026	1.934AsnArg: 1.934 ± 0.042	1.291AsnSer: 1.291 ± 0.035	1.181AsnThr: 1.181 ± 0.031	1.94AsnVal: 1.94 ± 0.044	0.439AsnTrp: 0.439 ± 0.021	0.725AsnTyr: 0.725 ± 0.027	0.002AsnXaa: 0.002 ± 0.001
Pro	5.668ProAla: 5.668 ± 0.081	0.327ProCys: 0.327 ± 0.017	3.542ProAsp: 3.542 ± 0.061	4.162ProGlu: 4.162 ± 0.066	2.056ProPhe: 2.056 ± 0.04	4.304ProGly: 4.304 ± 0.065	1.05ProHis: 1.05 ± 0.029	2.425ProIle: 2.425 ± 0.047	1.692ProLys: 1.692 ± 0.036	4.533ProLeu: 4.533 ± 0.062	1.162ProMet: 1.162 ± 0.031	1.366ProAsn: 1.366 ± 0.034	2.412ProPro: 2.412 ± 0.066	1.584ProGln: 1.584 ± 0.038	2.916ProArg: 2.916 ± 0.046	2.827ProSer: 2.827 ± 0.045	2.177ProThr: 2.177 ± 0.041	4.299ProVal: 4.299 ± 0.058	0.661ProTrp: 0.661 ± 0.026	1.238ProTyr: 1.238 ± 0.033	0.01ProXaa: 0.01 ± 0.003
Gln	3.724GlnAla: 3.724 ± 0.068	0.182GlnCys: 0.182 ± 0.013	1.351GlnAsp: 1.351 ± 0.033	1.783GlnGlu: 1.783 ± 0.043	1.083GlnPhe: 1.083 ± 0.034	2.192GlnGly: 2.192 ± 0.044	0.584GlnHis: 0.584 ± 0.019	1.842GlnIle: 1.842 ± 0.039	1.135GlnLys: 1.135 ± 0.032	2.527GlnLeu: 2.527 ± 0.046	0.889GlnMet: 0.889 ± 0.027	0.844GlnAsn: 0.844 ± 0.026	1.644GlnPro: 1.644 ± 0.04	1.038GlnGln: 1.038 ± 0.033	2.237GlnArg: 2.237 ± 0.046	1.677GlnSer: 1.677 ± 0.037	1.57GlnThr: 1.57 ± 0.039	2.082GlnVal: 2.082 ± 0.042	0.359GlnTrp: 0.359 ± 0.017	0.599GlnTyr: 0.599 ± 0.021	0.005GlnXaa: 0.005 ± 0.002
Arg	7.298ArgAla: 7.298 ± 0.08	0.496ArgCys: 0.496 ± 0.022	4.007ArgAsp: 4.007 ± 0.062	4.802ArgGlu: 4.802 ± 0.065	3.113ArgPhe: 3.113 ± 0.052	4.785ArgGly: 4.785 ± 0.065	1.796ArgHis: 1.796 ± 0.043	4.427ArgIle: 4.427 ± 0.065	2.778ArgLys: 2.778 ± 0.051	8.004ArgLeu: 8.004 ± 0.093	2.067ArgMet: 2.067 ± 0.038	2.068ArgAsn: 2.068 ± 0.043	3.594ArgPro: 3.594 ± 0.06	2.669ArgGln: 2.669 ± 0.049	6.13ArgArg: 6.13 ± 0.09	3.859ArgSer: 3.859 ± 0.058	3.375ArgThr: 3.375 ± 0.051	4.459ArgVal: 4.459 ± 0.064	0.969ArgTrp: 0.969 ± 0.029	1.823ArgTyr: 1.823 ± 0.038	0.012ArgXaa: 0.012 ± 0.003
Ser	6.048SerAla: 6.048 ± 0.08	0.398SerCys: 0.398 ± 0.02	3.007SerAsp: 3.007 ± 0.051	3.242SerGlu: 3.242 ± 0.055	2.384SerPhe: 2.384 ± 0.046	5.767SerGly: 5.767 ± 0.078	1.078SerHis: 1.078 ± 0.028	3.02SerIle: 3.02 ± 0.044	1.581SerLys: 1.581 ± 0.035	5.343SerLeu: 5.343 ± 0.078	1.391SerMet: 1.391 ± 0.037	1.352SerAsn: 1.352 ± 0.033	2.725SerPro: 2.725 ± 0.053	1.584SerGln: 1.584 ± 0.042	3.89SerArg: 3.89 ± 0.067	3.089SerSer: 3.089 ± 0.056	2.616SerThr: 2.616 ± 0.049	4.018SerVal: 4.018 ± 0.06	0.739SerTrp: 0.739 ± 0.024	1.312SerTyr: 1.312 ± 0.034	0.002SerXaa: 0.002 ± 0.001
Thr	5.907ThrAla: 5.907 ± 0.07	0.4ThrCys: 0.4 ± 0.02	2.729ThrAsp: 2.729 ± 0.053	2.855ThrGlu: 2.855 ± 0.048	1.944ThrPhe: 1.944 ± 0.039	5.256ThrGly: 5.256 ± 0.068	0.961ThrHis: 0.961 ± 0.028	3.122ThrIle: 3.122 ± 0.057	1.444ThrLys: 1.444 ± 0.039	5.165ThrLeu: 5.165 ± 0.073	1.332ThrMet: 1.332 ± 0.032	1.311ThrAsn: 1.311 ± 0.032	2.851ThrPro: 2.851 ± 0.052	1.209ThrGln: 1.209 ± 0.032	3.255ThrArg: 3.255 ± 0.053	2.678ThrSer: 2.678 ± 0.047	2.737ThrThr: 2.737 ± 0.054	4.404ThrVal: 4.404 ± 0.063	0.602ThrTrp: 0.602 ± 0.026	1.181ThrTyr: 1.181 ± 0.028	0.004ThrXaa: 0.004 ± 0.002
Val	8.618ValAla: 8.618 ± 0.085	0.58ValCys: 0.58 ± 0.024	3.959ValAsp: 3.959 ± 0.063	5.135ValGlu: 5.135 ± 0.062	2.897ValPhe: 2.897 ± 0.042	5.461ValGly: 5.461 ± 0.075	1.405ValHis: 1.405 ± 0.039	4.198ValIle: 4.198 ± 0.064	2.376ValLys: 2.376 ± 0.047	7.216ValLeu: 7.216 ± 0.08	1.97ValMet: 1.97 ± 0.043	1.991ValAsn: 1.991 ± 0.046	3.801ValPro: 3.801 ± 0.057	1.853ValGln: 1.853 ± 0.037	4.894ValArg: 4.894 ± 0.054	4.415ValSer: 4.415 ± 0.068	4.444ValThr: 4.444 ± 0.059	5.685ValVal: 5.685 ± 0.069	0.909ValTrp: 0.909 ± 0.03	1.578ValTyr: 1.578 ± 0.038	0.006ValXaa: 0.006 ± 0.002
Trp	1.177TrpAla: 1.177 ± 0.036	0.146TrpCys: 0.146 ± 0.009	0.61TrpAsp: 0.61 ± 0.024	0.585TrpGlu: 0.585 ± 0.024	0.532TrpPhe: 0.532 ± 0.02	0.856TrpGly: 0.856 ± 0.026	0.327TrpHis: 0.327 ± 0.017	0.685TrpIle: 0.685 ± 0.027	0.518TrpLys: 0.518 ± 0.02	1.55TrpLeu: 1.55 ± 0.042	0.366TrpMet: 0.366 ± 0.019	0.464TrpAsn: 0.464 ± 0.018	0.688TrpPro: 0.688 ± 0.028	0.528TrpGln: 0.528 ± 0.02	1.127TrpArg: 1.127 ± 0.035	0.789TrpSer: 0.789 ± 0.024	0.742TrpThr: 0.742 ± 0.028	0.761TrpVal: 0.761 ± 0.027	0.21TrpTrp: 0.21 ± 0.015	0.282TrpTyr: 0.282 ± 0.016	0.003TrpXaa: 0.003 ± 0.002
Tyr	2.39TyrAla: 2.39 ± 0.042	0.263TyrCys: 0.263 ± 0.015	1.479TyrAsp: 1.479 ± 0.036	1.385TyrGlu: 1.385 ± 0.035	0.959TyrPhe: 0.959 ± 0.03	2.299TyrGly: 2.299 ± 0.045	0.537TyrHis: 0.537 ± 0.021	0.975TyrIle: 0.975 ± 0.032	0.63TyrLys: 0.63 ± 0.021	2.332TyrLeu: 2.332 ± 0.044	0.5TyrMet: 0.5 ± 0.022	0.606TyrAsn: 0.606 ± 0.024	1.142TyrPro: 1.142 ± 0.03	0.689TyrGln: 0.689 ± 0.024	1.871TyrArg: 1.871 ± 0.038	1.292TyrSer: 1.292 ± 0.032	1.093TyrThr: 1.093 ± 0.03	1.684TyrVal: 1.684 ± 0.035	0.343TyrTrp: 0.343 ± 0.017	0.598TyrTyr: 0.598 ± 0.026	0.003TyrXaa: 0.003 ± 0.002
Xaa	0.025XaaAla: 0.025 ± 0.006	0.001XaaCys: 0.001 ± 0.001	0.004XaaAsp: 0.004 ± 0.001	0.01XaaGlu: 0.01 ± 0.003	0.001XaaPhe: 0.001 ± 0.001	0.008XaaGly: 0.008 ± 0.003	0.006XaaHis: 0.006 ± 0.002	0.003XaaIle: 0.003 ± 0.002	0.002XaaLys: 0.002 ± 0.001	0.007XaaLeu: 0.007 ± 0.002	0.001XaaMet: 0.001 ± 0.001	0.002XaaAsn: 0.002 ± 0.001	0.014XaaPro: 0.014 ± 0.003	0.002XaaGln: 0.002 ± 0.001	0.007XaaArg: 0.007 ± 0.002	0.006XaaSer: 0.006 ± 0.002	0.004XaaThr: 0.004 ± 0.002	0.006XaaVal: 0.006 ± 0.003	0.002XaaTrp: 0.002 ± 0.001	0.002XaaTyr: 0.002 ± 0.001	0.036XaaXaa: 0.036 ± 0.015

Statistics based on 4171 proteins (1255211 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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