Histophilus somni (strain 2336) (Haemophilus somnus)
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1971 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|B0UWR3|DNAJ_HISS2 Chaperone protein DnaJ OS=Histophilus somni (strain 2336) OX=228400 GN=dnaJ PE=3 SV=1
MM1 pKa = 7.65 AIVGLFYY8 pKa = 11.01 GSDD11 pKa = 3.2 TGNTEE16 pKa = 4.47 NIAKK20 pKa = 9.24 MIQKK24 pKa = 9.59 QLGSEE29 pKa = 4.49 LIDD32 pKa = 3.17 IRR34 pKa = 11.84 DD35 pKa = 3.35 IAKK38 pKa = 10.31 SSKK41 pKa = 10.43 EE42 pKa = 4.02 DD43 pKa = 3.21 IEE45 pKa = 5.67 SYY47 pKa = 10.87 DD48 pKa = 3.5 FLMIGIPTWYY58 pKa = 9.99 YY59 pKa = 11.6 GEE61 pKa = 4.69 AQCDD65 pKa = 3.26 WDD67 pKa = 5.24 DD68 pKa = 4.09 FFPTLEE74 pKa = 4.91 EE75 pKa = 4.17 IDD77 pKa = 4.0 FTDD80 pKa = 3.19 KK81 pKa = 10.88 LVAIFGCGDD90 pKa = 3.25 QEE92 pKa = 5.04 DD93 pKa = 4.3 YY94 pKa = 11.79 AEE96 pKa = 4.32 YY97 pKa = 10.5 FCDD100 pKa = 4.34 AMGTVRR106 pKa = 11.84 NIIEE110 pKa = 4.26 PKK112 pKa = 8.68 GAIIVGHH119 pKa = 6.89 WPTEE123 pKa = 3.95 GYY125 pKa = 8.67 TFEE128 pKa = 4.28 VSQALVDD135 pKa = 4.36 DD136 pKa = 4.01 NTFVGLCIDD145 pKa = 4.19 EE146 pKa = 4.82 DD147 pKa = 3.92 RR148 pKa = 11.84 QPEE151 pKa = 4.14 LTAEE155 pKa = 4.16 RR156 pKa = 11.84 VEE158 pKa = 4.08 KK159 pKa = 9.67 WVKK162 pKa = 9.55 QVYY165 pKa = 10.7 DD166 pKa = 4.08 EE167 pKa = 4.4 MCLAEE172 pKa = 4.39 LAA174 pKa = 4.24
Molecular weight: 19.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.897
IPC_protein 3.872
Toseland 3.668
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.706
Grimsley 3.579
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.986
Patrickios 1.888
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|B0URV6|B0URV6_HISS2 Competence protein OS=Histophilus somni (strain 2336) OX=228400 GN=HSM_0511 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.68 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.28 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1971
0
1971
655091
32
4656
332.4
37.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.916 ± 0.064
0.98 ± 0.027
5.024 ± 0.043
6.303 ± 0.053
4.277 ± 0.049
6.822 ± 0.096
2.057 ± 0.032
7.427 ± 0.054
6.765 ± 0.082
10.203 ± 0.092
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.3 ± 0.039
5.146 ± 0.07
3.462 ± 0.045
4.815 ± 0.063
4.216 ± 0.067
6.096 ± 0.064
5.443 ± 0.076
6.466 ± 0.046
1.073 ± 0.025
3.207 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here