Enterobacterales bacterium CwR94

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; unclassified Enterobacterales

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4133 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I0FR38|A0A2I0FR38_9GAMM Starvation-sensing protein RspA OS=Enterobacterales bacterium CwR94 OX=2025587 GN=CIG19_19135 PE=4 SV=1
MM1 pKa = 7.49AKK3 pKa = 9.87IGIFVGTVYY12 pKa = 11.16GNALLVAEE20 pKa = 4.78EE21 pKa = 4.15AQEE24 pKa = 3.92ILQSAGHH31 pKa = 5.54EE32 pKa = 4.07VGVFEE37 pKa = 6.61DD38 pKa = 3.84PTFEE42 pKa = 4.05NWTAYY47 pKa = 9.69QDD49 pKa = 3.65QVALIVTSTTGQGDD63 pKa = 3.89LPDD66 pKa = 4.56SIRR69 pKa = 11.84EE70 pKa = 4.04LFAALQDD77 pKa = 3.66QYY79 pKa = 11.65QPSLRR84 pKa = 11.84YY85 pKa = 9.62GVIALGDD92 pKa = 3.48SSYY95 pKa = 11.86DD96 pKa = 3.69DD97 pKa = 3.46FCGAGKK103 pKa = 10.56KK104 pKa = 9.69FDD106 pKa = 4.87ALLQEE111 pKa = 4.29QAAQRR116 pKa = 11.84VGDD119 pKa = 4.05VLLVDD124 pKa = 3.73AGEE127 pKa = 4.19YY128 pKa = 10.02PEE130 pKa = 4.74PEE132 pKa = 4.51EE133 pKa = 4.21VTSPWVEE140 pKa = 3.76SWAAQLL146 pKa = 4.2

Molecular weight: 15.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I0FW35|A0A2I0FW35_9GAMM LysR family transcriptional regulator OS=Enterobacterales bacterium CwR94 OX=2025587 GN=CIG19_09370 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSKK46 pKa = 11.03

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4133

0

4133

1317768

23

5454

318.8

35.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.293 ± 0.045

0.993 ± 0.014

5.106 ± 0.028

5.426 ± 0.042

3.726 ± 0.03

7.422 ± 0.048

2.308 ± 0.018

5.512 ± 0.03

3.861 ± 0.036

11.079 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.023

3.63 ± 0.041

4.472 ± 0.026

4.918 ± 0.036

5.762 ± 0.04

5.976 ± 0.032

5.523 ± 0.047

7.113 ± 0.028

1.558 ± 0.019

2.633 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski