Amino acid dipepetide frequency for Enterobacterales bacterium CwR94

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.214AlaAla: 11.214 ± 0.121	1.034AlaCys: 1.034 ± 0.031	5.14AlaAsp: 5.14 ± 0.069	6.151AlaGlu: 6.151 ± 0.078	3.711AlaPhe: 3.711 ± 0.058	8.094AlaGly: 8.094 ± 0.123	2.048AlaHis: 2.048 ± 0.038	5.91AlaIle: 5.91 ± 0.078	3.598AlaLys: 3.598 ± 0.062	13.035AlaLeu: 13.035 ± 0.132	3.104AlaMet: 3.104 ± 0.052	3.188AlaAsn: 3.188 ± 0.049	3.838AlaPro: 3.838 ± 0.062	5.15AlaGln: 5.15 ± 0.075	5.789AlaArg: 5.789 ± 0.069	5.817AlaSer: 5.817 ± 0.087	5.28AlaThr: 5.28 ± 0.085	7.142AlaVal: 7.142 ± 0.084	1.682AlaTrp: 1.682 ± 0.036	2.004AlaTyr: 2.004 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	0.965CysAla: 0.965 ± 0.03	0.162CysCys: 0.162 ± 0.01	0.58CysAsp: 0.58 ± 0.023	0.52CysGlu: 0.52 ± 0.019	0.408CysPhe: 0.408 ± 0.017	0.971CysGly: 0.971 ± 0.028	0.288CysHis: 0.288 ± 0.015	0.46CysIle: 0.46 ± 0.02	0.272CysLys: 0.272 ± 0.014	1.012CysLeu: 1.012 ± 0.03	0.214CysMet: 0.214 ± 0.013	0.298CysAsn: 0.298 ± 0.015	0.435CysPro: 0.435 ± 0.023	0.469CysGln: 0.469 ± 0.018	0.598CysArg: 0.598 ± 0.021	0.587CysSer: 0.587 ± 0.023	0.454CysThr: 0.454 ± 0.02	0.727CysVal: 0.727 ± 0.028	0.206CysTrp: 0.206 ± 0.012	0.305CysTyr: 0.305 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	5.692AspAla: 5.692 ± 0.08	0.47AspCys: 0.47 ± 0.023	2.784AspAsp: 2.784 ± 0.055	3.243AspGlu: 3.243 ± 0.058	2.069AspPhe: 2.069 ± 0.044	3.602AspGly: 3.602 ± 0.091	0.993AspHis: 0.993 ± 0.031	3.334AspIle: 3.334 ± 0.056	2.296AspLys: 2.296 ± 0.047	4.535AspLeu: 4.535 ± 0.061	1.207AspMet: 1.207 ± 0.033	2.232AspAsn: 2.232 ± 0.052	2.174AspPro: 2.174 ± 0.041	1.679AspGln: 1.679 ± 0.036	2.797AspArg: 2.797 ± 0.048	2.892AspSer: 2.892 ± 0.051	2.582AspThr: 2.582 ± 0.046	3.867AspVal: 3.867 ± 0.057	0.826AspTrp: 0.826 ± 0.029	1.78AspTyr: 1.78 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.638GluAla: 5.638 ± 0.085	0.442GluCys: 0.442 ± 0.018	2.249GluAsp: 2.249 ± 0.047	3.107GluGlu: 3.107 ± 0.067	1.639GluPhe: 1.639 ± 0.038	3.707GluGly: 3.707 ± 0.053	1.389GluHis: 1.389 ± 0.031	3.168GluIle: 3.168 ± 0.054	2.991GluLys: 2.991 ± 0.054	5.44GluLeu: 5.44 ± 0.082	1.7GluMet: 1.7 ± 0.042	2.189GluAsn: 2.189 ± 0.041	1.994GluPro: 1.994 ± 0.045	3.461GluGln: 3.461 ± 0.054	3.568GluArg: 3.568 ± 0.061	2.948GluSer: 2.948 ± 0.05	2.95GluThr: 2.95 ± 0.048	3.613GluVal: 3.613 ± 0.063	0.762GluTrp: 0.762 ± 0.026	1.304GluTyr: 1.304 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	3.685PheAla: 3.685 ± 0.056	0.483PheCys: 0.483 ± 0.019	2.286PheAsp: 2.286 ± 0.043	1.661PheGlu: 1.661 ± 0.035	1.579PhePhe: 1.579 ± 0.043	3.045PheGly: 3.045 ± 0.056	0.814PheHis: 0.814 ± 0.028	2.344PheIle: 2.344 ± 0.048	1.182PheLys: 1.182 ± 0.031	3.265PheLeu: 3.265 ± 0.062	0.931PheMet: 0.931 ± 0.028	1.645PheAsn: 1.645 ± 0.037	1.539PhePro: 1.539 ± 0.034	1.292PheGln: 1.292 ± 0.031	1.833PheArg: 1.833 ± 0.041	3.018PheSer: 3.018 ± 0.051	2.464PheThr: 2.464 ± 0.043	2.409PheVal: 2.409 ± 0.051	0.644PheTrp: 0.644 ± 0.025	1.14PheTyr: 1.14 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	6.545GlyAla: 6.545 ± 0.094	0.929GlyCys: 0.929 ± 0.028	3.765GlyAsp: 3.765 ± 0.068	4.621GlyGlu: 4.621 ± 0.061	3.199GlyPhe: 3.199 ± 0.054	5.339GlyGly: 5.339 ± 0.086	1.594GlyHis: 1.594 ± 0.037	4.969GlyIle: 4.969 ± 0.07	3.809GlyLys: 3.809 ± 0.06	7.573GlyLeu: 7.573 ± 0.09	2.295GlyMet: 2.295 ± 0.044	2.834GlyAsn: 2.834 ± 0.086	2.012GlyPro: 2.012 ± 0.038	3.114GlyGln: 3.114 ± 0.063	3.778GlyArg: 3.778 ± 0.052	4.358GlySer: 4.358 ± 0.084	3.909GlyThr: 3.909 ± 0.092	5.734GlyVal: 5.734 ± 0.074	1.373GlyTrp: 1.373 ± 0.035	2.477GlyTyr: 2.477 ± 0.041	0.0GlyXaa: 0.0 ± 0.0
His	2.205HisAla: 2.205 ± 0.044	0.34HisCys: 0.34 ± 0.017	1.133HisAsp: 1.133 ± 0.028	1.09HisGlu: 1.09 ± 0.031	1.048HisPhe: 1.048 ± 0.03	1.58HisGly: 1.58 ± 0.035	0.82HisHis: 0.82 ± 0.027	1.246HisIle: 1.246 ± 0.028	0.666HisLys: 0.666 ± 0.023	2.395HisLeu: 2.395 ± 0.04	0.51HisMet: 0.51 ± 0.019	0.79HisAsn: 0.79 ± 0.024	1.413HisPro: 1.413 ± 0.032	1.357HisGln: 1.357 ± 0.031	1.342HisArg: 1.342 ± 0.036	1.286HisSer: 1.286 ± 0.034	1.106HisThr: 1.106 ± 0.03	1.329HisVal: 1.329 ± 0.033	0.482HisTrp: 0.482 ± 0.02	0.947HisTyr: 0.947 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.523IleAla: 6.523 ± 0.079	0.615IleCys: 0.615 ± 0.02	3.321IleAsp: 3.321 ± 0.058	3.157IleGlu: 3.157 ± 0.058	1.882IlePhe: 1.882 ± 0.046	4.481IleGly: 4.481 ± 0.075	1.153IleHis: 1.153 ± 0.03	3.032IleIle: 3.032 ± 0.062	2.14IleLys: 2.14 ± 0.044	4.761IleLeu: 4.761 ± 0.073	1.209IleMet: 1.209 ± 0.035	2.439IleAsn: 2.439 ± 0.051	2.52IlePro: 2.52 ± 0.042	1.863IleGln: 1.863 ± 0.039	2.875IleArg: 2.875 ± 0.049	3.642IleSer: 3.642 ± 0.051	3.64IleThr: 3.64 ± 0.06	3.79IleVal: 3.79 ± 0.06	0.681IleTrp: 0.681 ± 0.025	1.395IleTyr: 1.395 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	3.922LysAla: 3.922 ± 0.075	0.225LysCys: 0.225 ± 0.014	1.817LysAsp: 1.817 ± 0.041	2.097LysGlu: 2.097 ± 0.049	0.995LysPhe: 0.995 ± 0.026	2.823LysGly: 2.823 ± 0.053	0.854LysHis: 0.854 ± 0.027	2.189LysIle: 2.189 ± 0.046	1.987LysLys: 1.987 ± 0.051	3.892LysLeu: 3.892 ± 0.057	1.143LysMet: 1.143 ± 0.03	1.531LysAsn: 1.531 ± 0.037	1.997LysPro: 1.997 ± 0.039	1.918LysGln: 1.918 ± 0.039	2.438LysArg: 2.438 ± 0.047	2.257LysSer: 2.257 ± 0.048	2.353LysThr: 2.353 ± 0.048	2.763LysVal: 2.763 ± 0.053	0.434LysTrp: 0.434 ± 0.018	0.969LysTyr: 0.969 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	11.908LeuAla: 11.908 ± 0.121	1.218LeuCys: 1.218 ± 0.032	5.416LeuAsp: 5.416 ± 0.074	5.194LeuGlu: 5.194 ± 0.073	4.281LeuPhe: 4.281 ± 0.067	7.378LeuGly: 7.378 ± 0.091	2.431LeuHis: 2.431 ± 0.043	5.81LeuIle: 5.81 ± 0.086	4.234LeuLys: 4.234 ± 0.06	12.504LeuLeu: 12.504 ± 0.176	3.084LeuMet: 3.084 ± 0.051	4.221LeuAsn: 4.221 ± 0.071	5.91LeuPro: 5.91 ± 0.074	5.018LeuGln: 5.018 ± 0.073	6.425LeuArg: 6.425 ± 0.078	7.871LeuSer: 7.871 ± 0.073	6.831LeuThr: 6.831 ± 0.09	7.074LeuVal: 7.074 ± 0.091	1.509LeuTrp: 1.509 ± 0.04	2.476LeuTyr: 2.476 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	2.893MetAla: 2.893 ± 0.044	0.168MetCys: 0.168 ± 0.013	1.16MetAsp: 1.16 ± 0.03	1.164MetGlu: 1.164 ± 0.034	0.817MetPhe: 0.817 ± 0.03	1.873MetGly: 1.873 ± 0.039	0.547MetHis: 0.547 ± 0.018	1.446MetIle: 1.446 ± 0.04	1.358MetLys: 1.358 ± 0.035	3.152MetLeu: 3.152 ± 0.056	0.939MetMet: 0.939 ± 0.029	1.014MetAsn: 1.014 ± 0.032	1.277MetPro: 1.277 ± 0.032	1.3MetGln: 1.3 ± 0.037	1.569MetArg: 1.569 ± 0.031	1.88MetSer: 1.88 ± 0.038	1.745MetThr: 1.745 ± 0.035	1.884MetVal: 1.884 ± 0.044	0.243MetTrp: 0.243 ± 0.015	0.464MetTyr: 0.464 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.87AsnAla: 3.87 ± 0.061	0.271AsnCys: 0.271 ± 0.014	1.937AsnAsp: 1.937 ± 0.039	1.67AsnGlu: 1.67 ± 0.034	1.189AsnPhe: 1.189 ± 0.03	3.059AsnGly: 3.059 ± 0.084	0.766AsnHis: 0.766 ± 0.023	2.125AsnIle: 2.125 ± 0.044	1.413AsnLys: 1.413 ± 0.04	3.522AsnLeu: 3.522 ± 0.055	0.848AsnMet: 0.848 ± 0.025	1.561AsnAsn: 1.561 ± 0.045	1.993AsnPro: 1.993 ± 0.043	1.657AsnGln: 1.657 ± 0.045	1.94AsnArg: 1.94 ± 0.037	2.071AsnSer: 2.071 ± 0.054	2.085AsnThr: 2.085 ± 0.051	2.618AsnVal: 2.618 ± 0.051	0.603AsnTrp: 0.603 ± 0.022	1.105AsnTyr: 1.105 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	4.929ProAla: 4.929 ± 0.071	0.349ProCys: 0.349 ± 0.016	2.68ProAsp: 2.68 ± 0.042	3.224ProGlu: 3.224 ± 0.059	1.799ProPhe: 1.799 ± 0.039	3.331ProGly: 3.331 ± 0.056	1.072ProHis: 1.072 ± 0.03	1.801ProIle: 1.801 ± 0.034	1.393ProLys: 1.393 ± 0.031	5.38ProLeu: 5.38 ± 0.067	1.122ProMet: 1.122 ± 0.031	1.292ProAsn: 1.292 ± 0.034	1.726ProPro: 1.726 ± 0.045	2.325ProGln: 2.325 ± 0.039	2.083ProArg: 2.083 ± 0.046	2.17ProSer: 2.17 ± 0.037	2.191ProThr: 2.191 ± 0.037	3.964ProVal: 3.964 ± 0.057	0.814ProTrp: 0.814 ± 0.027	1.079ProTyr: 1.079 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	5.301GlnAla: 5.301 ± 0.069	0.394GlnCys: 0.394 ± 0.02	2.016GlnAsp: 2.016 ± 0.04	2.154GlnGlu: 2.154 ± 0.045	1.492GlnPhe: 1.492 ± 0.039	3.583GlnGly: 3.583 ± 0.064	1.539GlnHis: 1.539 ± 0.037	2.289GlnIle: 2.289 ± 0.046	1.638GlnLys: 1.638 ± 0.041	5.551GlnLeu: 5.551 ± 0.076	1.263GlnMet: 1.263 ± 0.029	1.402GlnAsn: 1.402 ± 0.035	2.526GlnPro: 2.526 ± 0.047	4.245GlnGln: 4.245 ± 0.099	3.664GlnArg: 3.664 ± 0.068	2.513GlnSer: 2.513 ± 0.049	2.359GlnThr: 2.359 ± 0.053	3.27GlnVal: 3.27 ± 0.055	0.794GlnTrp: 0.794 ± 0.026	1.184GlnTyr: 1.184 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	4.869ArgAla: 4.869 ± 0.062	0.56ArgCys: 0.56 ± 0.021	3.243ArgAsp: 3.243 ± 0.051	3.832ArgGlu: 3.832 ± 0.068	2.608ArgPhe: 2.608 ± 0.047	3.486ArgGly: 3.486 ± 0.057	1.587ArgHis: 1.587 ± 0.044	3.312ArgIle: 3.312 ± 0.055	2.133ArgLys: 2.133 ± 0.042	6.656ArgLeu: 6.656 ± 0.091	1.569ArgMet: 1.569 ± 0.033	2.015ArgAsn: 2.015 ± 0.042	2.268ArgPro: 2.268 ± 0.045	3.329ArgGln: 3.329 ± 0.06	3.535ArgArg: 3.535 ± 0.07	2.731ArgSer: 2.731 ± 0.046	2.603ArgThr: 2.603 ± 0.043	4.065ArgVal: 4.065 ± 0.057	1.062ArgTrp: 1.062 ± 0.031	2.158ArgTyr: 2.158 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	6.286SerAla: 6.286 ± 0.082	0.536SerCys: 0.536 ± 0.023	3.086SerAsp: 3.086 ± 0.055	3.167SerGlu: 3.167 ± 0.053	2.135SerPhe: 2.135 ± 0.047	5.41SerGly: 5.41 ± 0.088	1.466SerHis: 1.466 ± 0.029	2.741SerIle: 2.741 ± 0.05	1.877SerLys: 1.877 ± 0.04	6.978SerLeu: 6.978 ± 0.086	1.493SerMet: 1.493 ± 0.038	1.939SerAsn: 1.939 ± 0.054	2.672SerPro: 2.672 ± 0.044	2.733SerGln: 2.733 ± 0.05	3.441SerArg: 3.441 ± 0.055	3.523SerSer: 3.523 ± 0.059	3.199SerThr: 3.199 ± 0.063	4.52SerVal: 4.52 ± 0.061	1.013SerTrp: 1.013 ± 0.031	1.546SerTyr: 1.546 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	5.518ThrAla: 5.518 ± 0.092	0.476ThrCys: 0.476 ± 0.019	2.673ThrAsp: 2.673 ± 0.05	2.684ThrGlu: 2.684 ± 0.046	2.072ThrPhe: 2.072 ± 0.035	4.53ThrGly: 4.53 ± 0.086	1.295ThrHis: 1.295 ± 0.031	2.5ThrIle: 2.5 ± 0.052	1.386ThrLys: 1.386 ± 0.039	8.388ThrLeu: 8.388 ± 0.107	0.999ThrMet: 0.999 ± 0.027	1.51ThrAsn: 1.51 ± 0.039	3.375ThrPro: 3.375 ± 0.056	2.629ThrGln: 2.629 ± 0.051	3.246ThrArg: 3.246 ± 0.056	2.912ThrSer: 2.912 ± 0.061	2.955ThrThr: 2.955 ± 0.069	4.124ThrVal: 4.124 ± 0.114	0.773ThrTrp: 0.773 ± 0.024	1.183ThrTyr: 1.183 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	7.452ValAla: 7.452 ± 0.089	0.706ValCys: 0.706 ± 0.025	3.713ValAsp: 3.713 ± 0.056	3.852ValGlu: 3.852 ± 0.067	2.541ValPhe: 2.541 ± 0.046	4.911ValGly: 4.911 ± 0.066	1.238ValHis: 1.238 ± 0.026	4.469ValIle: 4.469 ± 0.074	2.941ValLys: 2.941 ± 0.053	7.394ValLeu: 7.394 ± 0.087	2.283ValMet: 2.283 ± 0.047	2.767ValAsn: 2.767 ± 0.054	3.068ValPro: 3.068 ± 0.051	2.584ValGln: 2.584 ± 0.047	3.723ValArg: 3.723 ± 0.051	4.797ValSer: 4.797 ± 0.066	4.535ValThr: 4.535 ± 0.102	5.597ValVal: 5.597 ± 0.089	1.005ValTrp: 1.005 ± 0.032	1.549ValTyr: 1.549 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.065TrpAla: 1.065 ± 0.03	0.2TrpCys: 0.2 ± 0.012	0.627TrpAsp: 0.627 ± 0.022	0.556TrpGlu: 0.556 ± 0.021	0.71TrpPhe: 0.71 ± 0.027	0.965TrpGly: 0.965 ± 0.029	0.492TrpHis: 0.492 ± 0.02	0.772TrpIle: 0.772 ± 0.024	0.473TrpLys: 0.473 ± 0.018	2.44TrpLeu: 2.44 ± 0.046	0.437TrpMet: 0.437 ± 0.018	0.485TrpAsn: 0.485 ± 0.019	0.703TrpPro: 0.703 ± 0.022	1.383TrpGln: 1.383 ± 0.036	1.149TrpArg: 1.149 ± 0.038	0.881TrpSer: 0.881 ± 0.033	0.589TrpThr: 0.589 ± 0.024	0.969TrpVal: 0.969 ± 0.03	0.25TrpTrp: 0.25 ± 0.014	0.435TrpTyr: 0.435 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.45TyrAla: 2.45 ± 0.043	0.353TyrCys: 0.353 ± 0.018	1.432TyrAsp: 1.432 ± 0.036	1.114TyrGlu: 1.114 ± 0.026	1.083TyrPhe: 1.083 ± 0.028	2.059TyrGly: 2.059 ± 0.045	0.697TyrHis: 0.697 ± 0.025	1.2TyrIle: 1.2 ± 0.03	0.82TyrLys: 0.82 ± 0.033	2.919TyrLeu: 2.919 ± 0.049	0.543TyrMet: 0.543 ± 0.023	0.951TyrAsn: 0.951 ± 0.03	1.283TyrPro: 1.283 ± 0.031	1.673TyrGln: 1.673 ± 0.041	1.829TyrArg: 1.829 ± 0.036	1.604TyrSer: 1.604 ± 0.036	1.399TyrThr: 1.399 ± 0.039	1.667TyrVal: 1.667 ± 0.038	0.423TyrTrp: 0.423 ± 0.017	0.832TyrTyr: 0.832 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4133 proteins (1317768 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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