Alkalihalobacillus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4) (Bacillus pseudofirmus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus; Alkalihalobacillus pseudofirmus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4310 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3FSL0|D3FSL0_ALKPO Putative threonine aldolase OS=Alkalihalobacillus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4) OX=398511 GN=BpOF4_09615 PE=3 SV=1
MM1 pKa = 7.51EE2 pKa = 5.28KK3 pKa = 10.55VLLFCDD9 pKa = 3.91PGIDD13 pKa = 3.64DD14 pKa = 4.09SVAIMFALLHH24 pKa = 6.48PDD26 pKa = 3.08IEE28 pKa = 4.83VVGIVSSYY36 pKa = 11.17GNVDD40 pKa = 3.77KK41 pKa = 11.17EE42 pKa = 4.15QATNNAAYY50 pKa = 10.66LLDD53 pKa = 3.85LAGRR57 pKa = 11.84LDD59 pKa = 3.62VPLIGAAAYY68 pKa = 7.84PLSGEE73 pKa = 3.98MAVYY77 pKa = 10.11YY78 pKa = 9.51PEE80 pKa = 3.76IHH82 pKa = 6.42GEE84 pKa = 4.05EE85 pKa = 4.37GLGPITPPDD94 pKa = 4.25TISGTLEE101 pKa = 3.47NFSRR105 pKa = 11.84VFSIIDD111 pKa = 3.52EE112 pKa = 4.61YY113 pKa = 11.14EE114 pKa = 4.49GEE116 pKa = 4.16LVIVDD121 pKa = 3.59VGRR124 pKa = 11.84QTSLASAFILGEE136 pKa = 4.02DD137 pKa = 3.83VMDD140 pKa = 3.91KK141 pKa = 11.19VKK143 pKa = 10.95AFYY146 pKa = 11.59VMGGAFLVPGNVTPLAEE163 pKa = 4.54ANFFGDD169 pKa = 4.45PIAANVVATRR179 pKa = 11.84AHH181 pKa = 6.49SLTIVPLNVTNYY193 pKa = 10.8AILTDD198 pKa = 3.87EE199 pKa = 5.4VIDD202 pKa = 4.14QITDD206 pKa = 3.18QEE208 pKa = 4.96YY209 pKa = 8.12NTFTPLIRR217 pKa = 11.84PIYY220 pKa = 9.88DD221 pKa = 3.56YY222 pKa = 11.22YY223 pKa = 11.62AEE225 pKa = 5.01AYY227 pKa = 9.56QEE229 pKa = 4.09LVLGIEE235 pKa = 4.43GAPFHH240 pKa = 7.34DD241 pKa = 4.08VVTLVALTNPEE252 pKa = 3.96LFDD255 pKa = 3.85FVEE258 pKa = 4.79RR259 pKa = 11.84EE260 pKa = 4.1VTVVDD265 pKa = 4.22DD266 pKa = 3.88PSVRR270 pKa = 11.84GLSVADD276 pKa = 4.26FRR278 pKa = 11.84PLSAEE283 pKa = 3.85EE284 pKa = 3.81EE285 pKa = 4.18ASIEE289 pKa = 4.07GAQIAMDD296 pKa = 4.18FNYY299 pKa = 10.71DD300 pKa = 3.2EE301 pKa = 5.13YY302 pKa = 11.11INIFIQVMKK311 pKa = 10.48MEE313 pKa = 4.72FF314 pKa = 3.09

Molecular weight: 34.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3FQI1|D3FQI1_ALKPO Uncharacterized protein OS=Alkalihalobacillus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4) OX=398511 GN=BpOF4_07975 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.46KK3 pKa = 8.43PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.12KK15 pKa = 8.46VHH17 pKa = 5.5GFRR20 pKa = 11.84TRR22 pKa = 11.84MSTKK26 pKa = 9.9NGRR29 pKa = 11.84NVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4310

0

4310

1191893

26

2613

276.5

31.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.281 ± 0.036

0.683 ± 0.012

5.144 ± 0.03

7.988 ± 0.052

4.396 ± 0.029

6.822 ± 0.038

2.237 ± 0.021

7.441 ± 0.039

6.278 ± 0.037

9.898 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.873 ± 0.019

4.073 ± 0.026

3.595 ± 0.022

3.811 ± 0.025

4.276 ± 0.028

6.054 ± 0.029

5.5 ± 0.024

7.218 ± 0.034

0.995 ± 0.014

3.437 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski