Amino acid dipepetide frequency for Alkalihalobacillus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4) (Bacillus pseudofirmus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.61AlaAla: 6.61 ± 0.089	0.619AlaCys: 0.619 ± 0.027	3.554AlaAsp: 3.554 ± 0.059	5.079AlaGlu: 5.079 ± 0.07	3.35AlaPhe: 3.35 ± 0.063	5.533AlaGly: 5.533 ± 0.079	1.413AlaHis: 1.413 ± 0.037	5.787AlaIle: 5.787 ± 0.072	4.543AlaLys: 4.543 ± 0.075	7.575AlaLeu: 7.575 ± 0.076	2.132AlaMet: 2.132 ± 0.05	2.693AlaAsn: 2.693 ± 0.05	2.211AlaPro: 2.211 ± 0.047	2.228AlaGln: 2.228 ± 0.045	2.85AlaArg: 2.85 ± 0.051	4.394AlaSer: 4.394 ± 0.057	3.633AlaThr: 3.633 ± 0.055	5.604AlaVal: 5.604 ± 0.083	0.617AlaTrp: 0.617 ± 0.026	2.389AlaTyr: 2.389 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.482CysAla: 0.482 ± 0.021	0.106CysCys: 0.106 ± 0.009	0.367CysAsp: 0.367 ± 0.02	0.502CysGlu: 0.502 ± 0.024	0.289CysPhe: 0.289 ± 0.015	0.641CysGly: 0.641 ± 0.024	0.193CysHis: 0.193 ± 0.012	0.494CysIle: 0.494 ± 0.021	0.319CysLys: 0.319 ± 0.016	0.635CysLeu: 0.635 ± 0.023	0.193CysMet: 0.193 ± 0.011	0.262CysAsn: 0.262 ± 0.016	0.335CysPro: 0.335 ± 0.016	0.221CysGln: 0.221 ± 0.014	0.276CysArg: 0.276 ± 0.016	0.48CysSer: 0.48 ± 0.022	0.373CysThr: 0.373 ± 0.019	0.394CysVal: 0.394 ± 0.017	0.054CysTrp: 0.054 ± 0.008	0.213CysTyr: 0.213 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.339AspAla: 3.339 ± 0.051	0.358AspCys: 0.358 ± 0.019	2.514AspAsp: 2.514 ± 0.052	4.704AspGlu: 4.704 ± 0.074	2.248AspPhe: 2.248 ± 0.04	3.347AspGly: 3.347 ± 0.054	1.409AspHis: 1.409 ± 0.042	3.741AspIle: 3.741 ± 0.056	2.838AspLys: 2.838 ± 0.051	5.188AspLeu: 5.188 ± 0.069	1.369AspMet: 1.369 ± 0.036	1.576AspAsn: 1.576 ± 0.04	2.077AspPro: 2.077 ± 0.043	2.432AspGln: 2.432 ± 0.05	2.447AspArg: 2.447 ± 0.05	2.631AspSer: 2.631 ± 0.048	2.483AspThr: 2.483 ± 0.047	4.175AspVal: 4.175 ± 0.055	0.578AspTrp: 0.578 ± 0.025	1.988AspTyr: 1.988 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	6.393GluAla: 6.393 ± 0.081	0.4GluCys: 0.4 ± 0.021	4.204GluAsp: 4.204 ± 0.067	8.383GluGlu: 8.383 ± 0.115	2.582GluPhe: 2.582 ± 0.05	4.888GluGly: 4.888 ± 0.067	1.695GluHis: 1.695 ± 0.037	5.092GluIle: 5.092 ± 0.074	5.754GluLys: 5.754 ± 0.071	7.856GluLeu: 7.856 ± 0.094	2.509GluMet: 2.509 ± 0.047	3.164GluAsn: 3.164 ± 0.061	2.268GluPro: 2.268 ± 0.047	3.637GluGln: 3.637 ± 0.06	4.139GluArg: 4.139 ± 0.06	3.791GluSer: 3.791 ± 0.056	3.994GluThr: 3.994 ± 0.06	5.907GluVal: 5.907 ± 0.085	0.914GluTrp: 0.914 ± 0.028	2.314GluTyr: 2.314 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	2.983PheAla: 2.983 ± 0.06	0.306PheCys: 0.306 ± 0.02	2.347PheAsp: 2.347 ± 0.046	2.896PheGlu: 2.896 ± 0.051	2.331PhePhe: 2.331 ± 0.059	3.136PheGly: 3.136 ± 0.067	0.946PheHis: 0.946 ± 0.028	3.88PheIle: 3.88 ± 0.081	2.318PheLys: 2.318 ± 0.046	4.403PheLeu: 4.403 ± 0.079	1.174PheMet: 1.174 ± 0.037	1.884PheAsn: 1.884 ± 0.04	1.504PhePro: 1.504 ± 0.032	1.426PheGln: 1.426 ± 0.033	1.503PheArg: 1.503 ± 0.036	3.093PheSer: 3.093 ± 0.052	2.608PheThr: 2.608 ± 0.045	3.092PheVal: 3.092 ± 0.062	0.451PheTrp: 0.451 ± 0.022	1.681PheTyr: 1.681 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	5.162GlyAla: 5.162 ± 0.08	0.593GlyCys: 0.593 ± 0.021	3.218GlyAsp: 3.218 ± 0.046	4.948GlyGlu: 4.948 ± 0.078	3.386GlyPhe: 3.386 ± 0.057	4.885GlyGly: 4.885 ± 0.083	1.436GlyHis: 1.436 ± 0.031	5.495GlyIle: 5.495 ± 0.082	4.06GlyLys: 4.06 ± 0.057	6.761GlyLeu: 6.761 ± 0.096	2.186GlyMet: 2.186 ± 0.045	2.435GlyAsn: 2.435 ± 0.06	1.774GlyPro: 1.774 ± 0.039	2.281GlyGln: 2.281 ± 0.05	2.84GlyArg: 2.84 ± 0.054	3.997GlySer: 3.997 ± 0.065	3.878GlyThr: 3.878 ± 0.066	5.412GlyVal: 5.412 ± 0.075	0.765GlyTrp: 0.765 ± 0.026	2.71GlyTyr: 2.71 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	1.483HisAla: 1.483 ± 0.042	0.185HisCys: 0.185 ± 0.013	1.081HisAsp: 1.081 ± 0.029	1.467HisGlu: 1.467 ± 0.034	1.064HisPhe: 1.064 ± 0.032	1.402HisGly: 1.402 ± 0.037	0.746HisHis: 0.746 ± 0.024	1.574HisIle: 1.574 ± 0.033	1.171HisLys: 1.171 ± 0.034	2.45HisLeu: 2.45 ± 0.053	0.558HisMet: 0.558 ± 0.024	0.799HisAsn: 0.799 ± 0.026	1.174HisPro: 1.174 ± 0.032	0.985HisGln: 0.985 ± 0.029	0.887HisArg: 0.887 ± 0.027	1.404HisSer: 1.404 ± 0.035	1.223HisThr: 1.223 ± 0.033	1.536HisVal: 1.536 ± 0.039	0.228HisTrp: 0.228 ± 0.013	0.953HisTyr: 0.953 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.646IleAla: 5.646 ± 0.082	0.55IleCys: 0.55 ± 0.018	4.323IleAsp: 4.323 ± 0.056	6.127IleGlu: 6.127 ± 0.08	2.93IlePhe: 2.93 ± 0.069	5.804IleGly: 5.804 ± 0.076	1.764IleHis: 1.764 ± 0.037	5.49IleIle: 5.49 ± 0.084	4.129IleLys: 4.129 ± 0.059	6.626IleLeu: 6.626 ± 0.098	1.728IleMet: 1.728 ± 0.036	3.128IleAsn: 3.128 ± 0.054	3.249IlePro: 3.249 ± 0.055	2.73IleGln: 2.73 ± 0.051	3.068IleArg: 3.068 ± 0.055	4.776IleSer: 4.776 ± 0.068	4.233IleThr: 4.233 ± 0.06	5.244IleVal: 5.244 ± 0.069	0.619IleTrp: 0.619 ± 0.025	2.247IleTyr: 2.247 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.341LysAla: 4.341 ± 0.066	0.273LysCys: 0.273 ± 0.016	3.309LysAsp: 3.309 ± 0.059	6.918LysGlu: 6.918 ± 0.077	1.45LysPhe: 1.45 ± 0.037	4.111LysGly: 4.111 ± 0.059	1.358LysHis: 1.358 ± 0.035	3.555LysIle: 3.555 ± 0.061	5.327LysLys: 5.327 ± 0.069	5.317LysLeu: 5.317 ± 0.072	2.059LysMet: 2.059 ± 0.044	2.516LysAsn: 2.516 ± 0.048	2.16LysPro: 2.16 ± 0.041	3.196LysGln: 3.196 ± 0.054	3.493LysArg: 3.493 ± 0.053	3.234LysSer: 3.234 ± 0.053	3.214LysThr: 3.214 ± 0.051	4.497LysVal: 4.497 ± 0.063	0.784LysTrp: 0.784 ± 0.027	1.667LysTyr: 1.667 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	7.602LeuAla: 7.602 ± 0.091	0.656LeuCys: 0.656 ± 0.026	5.065LeuAsp: 5.065 ± 0.071	6.936LeuGlu: 6.936 ± 0.083	4.918LeuPhe: 4.918 ± 0.093	6.427LeuGly: 6.427 ± 0.089	2.084LeuHis: 2.084 ± 0.041	7.426LeuIle: 7.426 ± 0.106	6.114LeuLys: 6.114 ± 0.076	10.382LeuLeu: 10.382 ± 0.136	2.682LeuMet: 2.682 ± 0.052	4.192LeuAsn: 4.192 ± 0.062	3.833LeuPro: 3.833 ± 0.056	3.343LeuGln: 3.343 ± 0.055	3.884LeuArg: 3.884 ± 0.063	6.917LeuSer: 6.917 ± 0.082	6.097LeuThr: 6.097 ± 0.07	6.53LeuVal: 6.53 ± 0.086	0.782LeuTrp: 0.782 ± 0.028	3.106LeuTyr: 3.106 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.074MetAla: 2.074 ± 0.047	0.128MetCys: 0.128 ± 0.01	1.425MetAsp: 1.425 ± 0.034	1.884MetGlu: 1.884 ± 0.045	1.101MetPhe: 1.101 ± 0.035	1.737MetGly: 1.737 ± 0.043	0.464MetHis: 0.464 ± 0.019	2.352MetIle: 2.352 ± 0.047	2.415MetLys: 2.415 ± 0.051	2.644MetLeu: 2.644 ± 0.048	0.932MetMet: 0.932 ± 0.033	1.744MetAsn: 1.744 ± 0.04	0.985MetPro: 0.985 ± 0.033	1.029MetGln: 1.029 ± 0.031	1.261MetArg: 1.261 ± 0.034	1.862MetSer: 1.862 ± 0.04	1.798MetThr: 1.798 ± 0.039	1.885MetVal: 1.885 ± 0.04	0.201MetTrp: 0.201 ± 0.01	0.81MetTyr: 0.81 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.492AsnAla: 2.492 ± 0.051	0.256AsnCys: 0.256 ± 0.016	2.203AsnAsp: 2.203 ± 0.041	3.791AsnGlu: 3.791 ± 0.069	1.363AsnPhe: 1.363 ± 0.036	3.114AsnGly: 3.114 ± 0.06	1.057AsnHis: 1.057 ± 0.027	2.793AsnIle: 2.793 ± 0.059	2.588AsnLys: 2.588 ± 0.049	3.402AsnLeu: 3.402 ± 0.056	1.093AsnMet: 1.093 ± 0.03	1.729AsnAsn: 1.729 ± 0.047	1.944AsnPro: 1.944 ± 0.043	2.102AsnGln: 2.102 ± 0.057	1.972AsnArg: 1.972 ± 0.043	1.993AsnSer: 1.993 ± 0.044	1.979AsnThr: 1.979 ± 0.044	3.021AsnVal: 3.021 ± 0.052	0.472AsnTrp: 0.472 ± 0.018	1.366AsnTyr: 1.366 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	2.517ProAla: 2.517 ± 0.046	0.221ProCys: 0.221 ± 0.014	1.957ProAsp: 1.957 ± 0.043	2.956ProGlu: 2.956 ± 0.045	2.022ProPhe: 2.022 ± 0.042	2.188ProGly: 2.188 ± 0.05	0.902ProHis: 0.902 ± 0.03	2.719ProIle: 2.719 ± 0.052	2.059ProLys: 2.059 ± 0.049	3.706ProLeu: 3.706 ± 0.062	0.909ProMet: 0.909 ± 0.03	1.639ProAsn: 1.639 ± 0.036	1.074ProPro: 1.074 ± 0.033	1.084ProGln: 1.084 ± 0.033	1.201ProArg: 1.201 ± 0.034	2.29ProSer: 2.29 ± 0.041	1.951ProThr: 1.951 ± 0.04	2.798ProVal: 2.798 ± 0.046	0.344ProTrp: 0.344 ± 0.021	1.411ProTyr: 1.411 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	3.085GlnAla: 3.085 ± 0.059	0.194GlnCys: 0.194 ± 0.015	1.613GlnAsp: 1.613 ± 0.038	2.962GlnGlu: 2.962 ± 0.056	1.626GlnPhe: 1.626 ± 0.038	2.138GlnGly: 2.138 ± 0.045	0.884GlnHis: 0.884 ± 0.029	2.45GlnIle: 2.45 ± 0.046	2.485GlnLys: 2.485 ± 0.049	4.281GlnLeu: 4.281 ± 0.068	1.276GlnMet: 1.276 ± 0.035	1.425GlnAsn: 1.425 ± 0.036	1.313GlnPro: 1.313 ± 0.035	1.82GlnGln: 1.82 ± 0.052	1.572GlnArg: 1.572 ± 0.038	2.389GlnSer: 2.389 ± 0.047	2.275GlnThr: 2.275 ± 0.04	2.634GlnVal: 2.634 ± 0.052	0.418GlnTrp: 0.418 ± 0.02	1.272GlnTyr: 1.272 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.785ArgAla: 2.785 ± 0.055	0.268ArgCys: 0.268 ± 0.017	2.327ArgAsp: 2.327 ± 0.045	3.662ArgGlu: 3.662 ± 0.069	1.981ArgPhe: 1.981 ± 0.04	2.738ArgGly: 2.738 ± 0.058	0.903ArgHis: 0.903 ± 0.03	3.048ArgIle: 3.048 ± 0.059	3.015ArgLys: 3.015 ± 0.053	4.226ArgLeu: 4.226 ± 0.068	1.355ArgMet: 1.355 ± 0.038	1.759ArgAsn: 1.759 ± 0.042	1.356ArgPro: 1.356 ± 0.04	1.67ArgGln: 1.67 ± 0.036	1.986ArgArg: 1.986 ± 0.044	2.433ArgSer: 2.433 ± 0.05	2.311ArgThr: 2.311 ± 0.044	2.903ArgVal: 2.903 ± 0.055	0.426ArgTrp: 0.426 ± 0.018	1.604ArgTyr: 1.604 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	3.825SerAla: 3.825 ± 0.056	0.399SerCys: 0.399 ± 0.019	2.811SerAsp: 2.811 ± 0.046	4.224SerGlu: 4.224 ± 0.076	3.293SerPhe: 3.293 ± 0.056	4.265SerGly: 4.265 ± 0.069	1.306SerHis: 1.306 ± 0.033	4.998SerIle: 4.998 ± 0.072	3.571SerLys: 3.571 ± 0.055	6.358SerLeu: 6.358 ± 0.079	1.85SerMet: 1.85 ± 0.043	2.537SerAsn: 2.537 ± 0.049	2.131SerPro: 2.131 ± 0.043	2.066SerGln: 2.066 ± 0.045	2.389SerArg: 2.389 ± 0.042	4.052SerSer: 4.052 ± 0.06	3.238SerThr: 3.238 ± 0.062	4.182SerVal: 4.182 ± 0.053	0.63SerTrp: 0.63 ± 0.028	2.413SerTyr: 2.413 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	4.004ThrAla: 4.004 ± 0.061	0.354ThrCys: 0.354 ± 0.018	2.719ThrAsp: 2.719 ± 0.051	3.709ThrGlu: 3.709 ± 0.065	2.788ThrPhe: 2.788 ± 0.047	3.997ThrGly: 3.997 ± 0.055	1.247ThrHis: 1.247 ± 0.031	4.538ThrIle: 4.538 ± 0.062	3.285ThrLys: 3.285 ± 0.046	5.498ThrLeu: 5.498 ± 0.065	1.391ThrMet: 1.391 ± 0.039	2.395ThrAsn: 2.395 ± 0.048	2.319ThrPro: 2.319 ± 0.043	1.585ThrGln: 1.585 ± 0.035	2.018ThrArg: 2.018 ± 0.041	3.398ThrSer: 3.398 ± 0.052	3.062ThrThr: 3.062 ± 0.056	4.015ThrVal: 4.015 ± 0.063	0.542ThrTrp: 0.542 ± 0.02	2.137ThrTyr: 2.137 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	5.24ValAla: 5.24 ± 0.076	0.618ValCys: 0.618 ± 0.026	3.993ValAsp: 3.993 ± 0.061	5.435ValGlu: 5.435 ± 0.08	3.037ValPhe: 3.037 ± 0.055	4.813ValGly: 4.813 ± 0.068	1.472ValHis: 1.472 ± 0.034	5.896ValIle: 5.896 ± 0.078	4.252ValLys: 4.252 ± 0.076	7.023ValLeu: 7.023 ± 0.085	2.101ValMet: 2.101 ± 0.044	3.056ValAsn: 3.056 ± 0.061	2.65ValPro: 2.65 ± 0.046	2.348ValGln: 2.348 ± 0.053	2.989ValArg: 2.989 ± 0.054	4.625ValSer: 4.625 ± 0.063	4.361ValThr: 4.361 ± 0.065	5.313ValVal: 5.313 ± 0.067	0.634ValTrp: 0.634 ± 0.023	2.327ValTyr: 2.327 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.612TrpAla: 0.612 ± 0.026	0.076TrpCys: 0.076 ± 0.008	0.498TrpAsp: 0.498 ± 0.022	0.672TrpGlu: 0.672 ± 0.024	0.519TrpPhe: 0.519 ± 0.025	0.633TrpGly: 0.633 ± 0.022	0.196TrpHis: 0.196 ± 0.013	0.761TrpIle: 0.761 ± 0.03	0.612TrpLys: 0.612 ± 0.02	1.182TrpLeu: 1.182 ± 0.042	0.385TrpMet: 0.385 ± 0.018	0.477TrpAsn: 0.477 ± 0.022	0.26TrpPro: 0.26 ± 0.016	0.367TrpGln: 0.367 ± 0.017	0.406TrpArg: 0.406 ± 0.02	0.658TrpSer: 0.658 ± 0.024	0.472TrpThr: 0.472 ± 0.02	0.69TrpVal: 0.69 ± 0.023	0.133TrpTrp: 0.133 ± 0.011	0.342TrpTyr: 0.342 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.138TyrAla: 2.138 ± 0.045	0.268TyrCys: 0.268 ± 0.015	1.914TyrAsp: 1.914 ± 0.043	2.628TyrGlu: 2.628 ± 0.046	1.686TyrPhe: 1.686 ± 0.045	2.426TyrGly: 2.426 ± 0.048	0.897TyrHis: 0.897 ± 0.029	2.322TyrIle: 2.322 ± 0.047	1.92TyrLys: 1.92 ± 0.043	3.463TyrLeu: 3.463 ± 0.062	0.848TyrMet: 0.848 ± 0.025	1.319TyrAsn: 1.319 ± 0.033	1.331TyrPro: 1.331 ± 0.033	1.56TyrGln: 1.56 ± 0.033	1.577TyrArg: 1.577 ± 0.043	2.124TyrSer: 2.124 ± 0.043	1.82TyrThr: 1.82 ± 0.041	2.35TyrVal: 2.35 ± 0.045	0.358TyrTrp: 0.358 ± 0.017	1.415TyrTyr: 1.415 ± 0.043	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4310 proteins (1191893 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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