Metarhizium anisopliae BRIP 53293

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium; Metarhizium anisopliae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11404 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D9P874|A0A0D9P874_METAN Uncharacterized protein OS=Metarhizium anisopliae BRIP 53293 OX=1291518 GN=H634G_02613 PE=4 SV=1
MM1 pKa = 7.71SDD3 pKa = 4.43DD4 pKa = 4.42EE5 pKa = 4.57NLSIYY10 pKa = 10.65DD11 pKa = 3.91EE12 pKa = 4.49IEE14 pKa = 4.29IEE16 pKa = 5.12DD17 pKa = 3.73MSFDD21 pKa = 3.65EE22 pKa = 5.51ALQLYY27 pKa = 9.0HH28 pKa = 6.45YY29 pKa = 7.63PCPCGDD35 pKa = 3.77RR36 pKa = 11.84FQIALDD42 pKa = 3.95DD43 pKa = 4.77LRR45 pKa = 11.84DD46 pKa = 3.68EE47 pKa = 4.38QDD49 pKa = 2.94IAVCPSCSLMIRR61 pKa = 11.84VIFDD65 pKa = 4.38LDD67 pKa = 4.33DD68 pKa = 4.16LPKK71 pKa = 10.42PPPSGNGAQVPIAAA85 pKa = 4.83

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D9NSE5|A0A0D9NSE5_METAN Eukaryotic translation initiation factor 6 OS=Metarhizium anisopliae BRIP 53293 OX=1291518 GN=TIF6 PE=3 SV=1
MM1 pKa = 7.53VKK3 pKa = 9.75QQLTPSCLFSGAAACMAVCFTHH25 pKa = 7.49PLDD28 pKa = 3.45QTKK31 pKa = 10.4YY32 pKa = 9.63RR33 pKa = 11.84MQVLKK38 pKa = 10.63SSNSMLSTMYY48 pKa = 10.68RR49 pKa = 11.84FGLRR53 pKa = 11.84DD54 pKa = 4.86GIPSLWGGLSASILRR69 pKa = 11.84QGTYY73 pKa = 7.97STARR77 pKa = 11.84FGLHH81 pKa = 5.43NHH83 pKa = 6.96LSSQLLQTSGRR94 pKa = 11.84DD95 pKa = 3.34RR96 pKa = 11.84LPASWNIACAGVSGGIAGLIGNPTEE121 pKa = 4.15VVLVRR126 pKa = 11.84MCADD130 pKa = 3.45GAKK133 pKa = 9.66PARR136 pKa = 11.84DD137 pKa = 2.99RR138 pKa = 11.84FRR140 pKa = 11.84YY141 pKa = 9.95SNPLVGLWRR150 pKa = 11.84VWKK153 pKa = 10.65EE154 pKa = 3.7EE155 pKa = 3.76GAKK158 pKa = 10.64AFGRR162 pKa = 11.84GIGPNVTRR170 pKa = 11.84SVLMNVSQIASYY182 pKa = 11.36ASAKK186 pKa = 10.06QSLLASNNMTDD197 pKa = 4.29NITTHH202 pKa = 6.77ALASLIAGTGLLQIVRR218 pKa = 11.84NGLRR222 pKa = 11.84DD223 pKa = 3.53EE224 pKa = 4.72GPRR227 pKa = 11.84FLMKK231 pKa = 10.29GWTPAWLRR239 pKa = 11.84LTPHH243 pKa = 6.13TVLTFVFMEE252 pKa = 3.71QLRR255 pKa = 11.84RR256 pKa = 11.84LSQLTVSQQQRR267 pKa = 11.84DD268 pKa = 3.68TAKK271 pKa = 9.5MM272 pKa = 3.43

Molecular weight: 29.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11404

0

11404

5535669

50

10258

485.4

53.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.901 ± 0.02

1.301 ± 0.009

5.896 ± 0.017

5.967 ± 0.025

3.668 ± 0.014

7.022 ± 0.022

2.425 ± 0.01

4.795 ± 0.016

4.819 ± 0.019

8.934 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.008

3.649 ± 0.013

5.87 ± 0.025

4.053 ± 0.017

6.168 ± 0.018

8.017 ± 0.024

5.808 ± 0.016

6.282 ± 0.017

1.485 ± 0.008

2.691 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski