Streptococcus phage CHPC1005
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8F8U2|A0A3G8F8U2_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1005 OX=2365005 GN=CHPC1005_0033 PE=4 SV=1
MM1 pKa = 7.12 VNWVDD6 pKa = 4.58 KK7 pKa = 11.1 DD8 pKa = 4.24 GNDD11 pKa = 3.97 LPDD14 pKa = 4.26 GADD17 pKa = 3.29 QEE19 pKa = 5.13 FKK21 pKa = 11.28 AGMFFSFAGDD31 pKa = 3.56 EE32 pKa = 4.15 NNITDD37 pKa = 3.82 TGEE40 pKa = 3.65 GGYY43 pKa = 10.61 YY44 pKa = 9.76 GGYY47 pKa = 8.0 YY48 pKa = 9.41 YY49 pKa = 10.76 RR50 pKa = 11.84 RR51 pKa = 11.84 FEE53 pKa = 4.06 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.617
ProMoST 4.024
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.948
Patrickios 0.477
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.851
Protein with the highest isoelectric point:
>tr|A0A3G8F8T9|A0A3G8F8T9_9CAUD N-acetylmuramoyl-L-alanine amidase OS=Streptococcus phage CHPC1005 OX=2365005 GN=CHPC1005_0027 PE=4 SV=1
MM1 pKa = 7.56 SKK3 pKa = 9.22 TKK5 pKa = 10.5 RR6 pKa = 11.84 GVCANCHH13 pKa = 4.61 TVFEE17 pKa = 4.52 VSKK20 pKa = 9.76 KK21 pKa = 7.91 QRR23 pKa = 11.84 YY24 pKa = 8.42 KK25 pKa = 10.36 IKK27 pKa = 9.76 NGKK30 pKa = 8.84 SVFCSQTCSLEE41 pKa = 4.28 KK42 pKa = 10.59 YY43 pKa = 9.48 GKK45 pKa = 8.13 TKK47 pKa = 9.25 ITISEE52 pKa = 4.39 IPCCRR57 pKa = 11.84 CGKK60 pKa = 9.23 MFIPTYY66 pKa = 9.85 SQYY69 pKa = 11.18 KK70 pKa = 8.15 RR71 pKa = 11.84 YY72 pKa = 10.09 KK73 pKa = 8.01 YY74 pKa = 10.86 NNYY77 pKa = 9.6 VSNSFCSNEE86 pKa = 3.99 CRR88 pKa = 11.84 WKK90 pKa = 10.71 KK91 pKa = 9.58 EE92 pKa = 3.98 YY93 pKa = 9.8 PYY95 pKa = 9.73 EE96 pKa = 3.96 LHH98 pKa = 7.28 DD99 pKa = 4.69 GYY101 pKa = 11.87 VSVFVNGKK109 pKa = 9.9 EE110 pKa = 3.7 ILLDD114 pKa = 3.19 VDD116 pKa = 4.06 VFEE119 pKa = 5.62 KK120 pKa = 10.88 YY121 pKa = 10.73 SKK123 pKa = 9.48 TLYY126 pKa = 8.88 VQNDD130 pKa = 2.67 KK131 pKa = 10.98 RR132 pKa = 11.84 SNYY135 pKa = 8.34 NSVMVFEE142 pKa = 4.77 EE143 pKa = 4.69 GKK145 pKa = 10.43 KK146 pKa = 10.03 RR147 pKa = 11.84 LSRR150 pKa = 11.84 LIMSVTDD157 pKa = 3.56 KK158 pKa = 11.12 NKK160 pKa = 10.88 SIDD163 pKa = 3.98 HH164 pKa = 6.59 INGNSLDD171 pKa = 3.69 NRR173 pKa = 11.84 RR174 pKa = 11.84 SNLRR178 pKa = 11.84 VVSHH182 pKa = 5.96 QEE184 pKa = 3.28 NMMNKK189 pKa = 8.37 ATYY192 pKa = 10.19 KK193 pKa = 10.88 NNTSEE198 pKa = 4.18 IKK200 pKa = 10.53 GVNLNKK206 pKa = 10.19 KK207 pKa = 8.12 GLWVARR213 pKa = 11.84 IQVRR217 pKa = 11.84 NQRR220 pKa = 11.84 IFLGSSKK227 pKa = 10.66 DD228 pKa = 3.3 KK229 pKa = 11.21 SVAEE233 pKa = 4.09 KK234 pKa = 10.85 LRR236 pKa = 11.84 TEE238 pKa = 4.03 AEE240 pKa = 3.72 KK241 pKa = 10.68 KK242 pKa = 10.73 YY243 pKa = 10.36 FGKK246 pKa = 10.57 YY247 pKa = 8.25 DD248 pKa = 3.53 RR249 pKa = 11.84 KK250 pKa = 10.2 YY251 pKa = 11.25 LKK253 pKa = 10.64
Molecular weight: 29.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.115
IPC2_protein 9.194
IPC_protein 9.077
Toseland 9.853
ProMoST 9.56
Dawson 10.072
Bjellqvist 9.75
Wikipedia 10.218
Rodwell 10.555
Grimsley 10.131
Solomon 10.087
Lehninger 10.043
Nozaki 9.911
DTASelect 9.721
Thurlkill 9.926
EMBOSS 10.262
Sillero 9.999
Patrickios 9.794
IPC_peptide 10.087
IPC2_peptide 8.448
IPC2.peptide.svr19 8.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
11388
35
1593
242.3
27.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.296 ± 0.541
0.711 ± 0.198
6.419 ± 0.264
6.735 ± 0.459
4.329 ± 0.211
6.744 ± 0.465
1.44 ± 0.103
6.665 ± 0.251
8.878 ± 0.549
7.842 ± 0.378
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.222 ± 0.187
6.577 ± 0.251
3.056 ± 0.197
4.022 ± 0.243
4.312 ± 0.264
6.243 ± 0.25
6.085 ± 0.346
5.91 ± 0.264
1.484 ± 0.166
4.031 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here