Amino acid dipepetide frequency for Streptococcus phage CHPC1005

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.635AlaAla: 2.635 ± 0.995
0.176AlaCys: 0.176 ± 0.123
4.303AlaAsp: 4.303 ± 0.779
3.425AlaGlu: 3.425 ± 0.473
2.371AlaPhe: 2.371 ± 0.574
3.864AlaGly: 3.864 ± 0.61
0.878AlaHis: 0.878 ± 0.271
4.918AlaIle: 4.918 ± 0.814
6.323AlaLys: 6.323 ± 1.176
5.884AlaLeu: 5.884 ± 0.759
1.493AlaMet: 1.493 ± 0.357
4.215AlaAsn: 4.215 ± 0.657
1.844AlaPro: 1.844 ± 0.332
2.459AlaGln: 2.459 ± 0.466
2.722AlaArg: 2.722 ± 0.455
4.479AlaSer: 4.479 ± 0.712
4.303AlaThr: 4.303 ± 0.775
3.337AlaVal: 3.337 ± 0.666
0.878AlaTrp: 0.878 ± 0.272
2.459AlaTyr: 2.459 ± 0.497
0.0AlaXaa: 0.0 ± 0.0
Cys
0.176CysAla: 0.176 ± 0.114
0.088CysCys: 0.088 ± 0.077
0.878CysAsp: 0.878 ± 0.367
0.439CysGlu: 0.439 ± 0.206
0.351CysPhe: 0.351 ± 0.21
0.263CysGly: 0.263 ± 0.152
0.176CysHis: 0.176 ± 0.107
0.351CysIle: 0.351 ± 0.178
0.527CysLys: 0.527 ± 0.275
0.439CysLeu: 0.439 ± 0.229
0.088CysMet: 0.088 ± 0.077
0.439CysAsn: 0.439 ± 0.258
0.439CysPro: 0.439 ± 0.228
0.176CysGln: 0.176 ± 0.114
0.79CysArg: 0.79 ± 0.365
0.615CysSer: 0.615 ± 0.262
0.263CysThr: 0.263 ± 0.149
0.176CysVal: 0.176 ± 0.099
0.176CysTrp: 0.176 ± 0.119
0.263CysTyr: 0.263 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
3.249AspAla: 3.249 ± 0.747
0.527AspCys: 0.527 ± 0.222
3.864AspAsp: 3.864 ± 0.571
4.04AspGlu: 4.04 ± 0.72
3.601AspPhe: 3.601 ± 0.546
5.972AspGly: 5.972 ± 1.031
1.142AspHis: 1.142 ± 0.3
4.567AspIle: 4.567 ± 0.717
4.918AspLys: 4.918 ± 0.681
4.128AspLeu: 4.128 ± 0.632
2.02AspMet: 2.02 ± 0.406
4.654AspAsn: 4.654 ± 0.946
1.932AspPro: 1.932 ± 0.43
1.229AspGln: 1.229 ± 0.272
3.249AspArg: 3.249 ± 0.53
3.513AspSer: 3.513 ± 0.692
3.864AspThr: 3.864 ± 0.629
3.864AspVal: 3.864 ± 0.691
0.966AspTrp: 0.966 ± 0.311
2.898AspTyr: 2.898 ± 0.473
0.0AspXaa: 0.0 ± 0.0
Glu
4.04GluAla: 4.04 ± 0.649
0.439GluCys: 0.439 ± 0.164
3.601GluAsp: 3.601 ± 0.56
4.479GluGlu: 4.479 ± 0.858
2.898GluPhe: 2.898 ± 0.556
3.425GluGly: 3.425 ± 0.487
0.966GluHis: 0.966 ± 0.317
5.972GluIle: 5.972 ± 0.801
4.918GluLys: 4.918 ± 1.167
6.411GluLeu: 6.411 ± 0.85
1.932GluMet: 1.932 ± 0.482
3.864GluAsn: 3.864 ± 0.521
1.932GluPro: 1.932 ± 0.531
3.161GluGln: 3.161 ± 0.592
2.986GluArg: 2.986 ± 0.584
3.337GluSer: 3.337 ± 0.572
3.337GluThr: 3.337 ± 0.565
5.006GluVal: 5.006 ± 0.819
1.142GluTrp: 1.142 ± 0.254
3.425GluTyr: 3.425 ± 0.713
0.0GluXaa: 0.0 ± 0.0
Phe
3.337PheAla: 3.337 ± 0.481
0.351PheCys: 0.351 ± 0.201
3.513PheAsp: 3.513 ± 0.586
2.547PheGlu: 2.547 ± 0.548
1.405PhePhe: 1.405 ± 0.316
3.249PheGly: 3.249 ± 0.689
0.615PheHis: 0.615 ± 0.182
2.635PheIle: 2.635 ± 0.53
4.215PheLys: 4.215 ± 0.492
3.074PheLeu: 3.074 ± 0.457
0.439PheMet: 0.439 ± 0.185
3.776PheAsn: 3.776 ± 0.672
0.527PhePro: 0.527 ± 0.203
1.054PheGln: 1.054 ± 0.271
1.669PheArg: 1.669 ± 0.336
2.898PheSer: 2.898 ± 0.519
3.161PheThr: 3.161 ± 0.688
2.371PheVal: 2.371 ± 0.405
0.703PheTrp: 0.703 ± 0.251
1.756PheTyr: 1.756 ± 0.361
0.0PheXaa: 0.0 ± 0.0
Gly
2.986GlyAla: 2.986 ± 0.508
0.615GlyCys: 0.615 ± 0.276
4.04GlyAsp: 4.04 ± 0.504
4.303GlyGlu: 4.303 ± 0.698
3.337GlyPhe: 3.337 ± 0.5
4.128GlyGly: 4.128 ± 0.726
0.79GlyHis: 0.79 ± 0.241
4.918GlyIle: 4.918 ± 0.706
7.289GlyLys: 7.289 ± 0.883
5.884GlyLeu: 5.884 ± 0.727
1.317GlyMet: 1.317 ± 0.324
4.215GlyAsn: 4.215 ± 0.785
1.229GlyPro: 1.229 ± 0.452
2.722GlyGln: 2.722 ± 0.663
2.635GlyArg: 2.635 ± 0.417
4.567GlySer: 4.567 ± 0.638
4.04GlyThr: 4.04 ± 0.769
3.864GlyVal: 3.864 ± 0.715
1.317GlyTrp: 1.317 ± 0.339
3.249GlyTyr: 3.249 ± 0.502
0.0GlyXaa: 0.0 ± 0.0
His
0.439HisAla: 0.439 ± 0.144
0.088HisCys: 0.088 ± 0.083
0.79HisAsp: 0.79 ± 0.301
0.527HisGlu: 0.527 ± 0.21
0.527HisPhe: 0.527 ± 0.207
0.878HisGly: 0.878 ± 0.26
0.439HisHis: 0.439 ± 0.169
1.142HisIle: 1.142 ± 0.3
1.142HisLys: 1.142 ± 0.351
1.317HisLeu: 1.317 ± 0.303
0.351HisMet: 0.351 ± 0.206
0.615HisAsn: 0.615 ± 0.215
0.527HisPro: 0.527 ± 0.205
0.615HisGln: 0.615 ± 0.25
0.966HisArg: 0.966 ± 0.242
1.142HisSer: 1.142 ± 0.26
0.615HisThr: 0.615 ± 0.218
1.405HisVal: 1.405 ± 0.265
0.088HisTrp: 0.088 ± 0.089
0.79HisTyr: 0.79 ± 0.245
0.0HisXaa: 0.0 ± 0.0
Ile
4.479IleAla: 4.479 ± 0.781
0.527IleCys: 0.527 ± 0.252
5.006IleAsp: 5.006 ± 0.684
4.83IleGlu: 4.83 ± 0.822
1.932IlePhe: 1.932 ± 0.425
4.479IleGly: 4.479 ± 0.616
0.439IleHis: 0.439 ± 0.174
3.776IleIle: 3.776 ± 0.562
7.377IleLys: 7.377 ± 0.908
3.864IleLeu: 3.864 ± 0.659
2.02IleMet: 2.02 ± 0.468
4.215IleAsn: 4.215 ± 0.514
3.425IlePro: 3.425 ± 0.57
2.635IleGln: 2.635 ± 0.418
3.074IleArg: 3.074 ± 0.553
4.391IleSer: 4.391 ± 0.609
3.601IleThr: 3.601 ± 0.661
3.337IleVal: 3.337 ± 0.574
0.878IleTrp: 0.878 ± 0.253
2.371IleTyr: 2.371 ± 0.52
0.0IleXaa: 0.0 ± 0.0
Lys
5.972LysAla: 5.972 ± 0.594
0.527LysCys: 0.527 ± 0.299
4.918LysAsp: 4.918 ± 0.701
7.816LysGlu: 7.816 ± 1.237
3.249LysPhe: 3.249 ± 0.758
6.411LysGly: 6.411 ± 0.744
1.317LysHis: 1.317 ± 0.419
5.796LysIle: 5.796 ± 0.9
8.343LysLys: 8.343 ± 1.308
6.499LysLeu: 6.499 ± 0.761
2.195LysMet: 2.195 ± 0.561
5.269LysAsn: 5.269 ± 0.612
2.81LysPro: 2.81 ± 0.389
3.952LysGln: 3.952 ± 0.522
4.303LysArg: 4.303 ± 0.594
4.479LysSer: 4.479 ± 0.612
5.796LysThr: 5.796 ± 0.702
4.128LysVal: 4.128 ± 0.687
1.317LysTrp: 1.317 ± 0.321
3.688LysTyr: 3.688 ± 0.811
0.0LysXaa: 0.0 ± 0.0
Leu
6.323LeuAla: 6.323 ± 0.941
0.527LeuCys: 0.527 ± 0.203
5.533LeuAsp: 5.533 ± 0.66
5.972LeuGlu: 5.972 ± 0.843
2.898LeuPhe: 2.898 ± 0.414
5.445LeuGly: 5.445 ± 1.076
0.878LeuHis: 0.878 ± 0.25
4.128LeuIle: 4.128 ± 0.57
7.201LeuLys: 7.201 ± 0.773
5.445LeuLeu: 5.445 ± 0.726
2.459LeuMet: 2.459 ± 0.407
5.708LeuAsn: 5.708 ± 0.606
2.722LeuPro: 2.722 ± 0.426
2.722LeuGln: 2.722 ± 0.464
4.128LeuArg: 4.128 ± 0.774
4.83LeuSer: 4.83 ± 0.765
5.445LeuThr: 5.445 ± 0.708
3.688LeuVal: 3.688 ± 0.593
0.703LeuTrp: 0.703 ± 0.267
1.669LeuTyr: 1.669 ± 0.423
0.0LeuXaa: 0.0 ± 0.0
Met
1.756MetAla: 1.756 ± 0.374
0.0MetCys: 0.0 ± 0.0
0.703MetAsp: 0.703 ± 0.225
1.405MetGlu: 1.405 ± 0.301
1.229MetPhe: 1.229 ± 0.278
0.878MetGly: 0.878 ± 0.279
0.263MetHis: 0.263 ± 0.167
1.669MetIle: 1.669 ± 0.398
2.371MetLys: 2.371 ± 0.492
1.581MetLeu: 1.581 ± 0.301
0.351MetMet: 0.351 ± 0.179
1.142MetAsn: 1.142 ± 0.282
0.966MetPro: 0.966 ± 0.266
0.966MetGln: 0.966 ± 0.26
0.703MetArg: 0.703 ± 0.248
1.844MetSer: 1.844 ± 0.462
1.317MetThr: 1.317 ± 0.277
2.02MetVal: 2.02 ± 0.458
0.088MetTrp: 0.088 ± 0.074
0.966MetTyr: 0.966 ± 0.301
0.0MetXaa: 0.0 ± 0.0
Asn
4.918AsnAla: 4.918 ± 1.04
0.351AsnCys: 0.351 ± 0.18
3.601AsnAsp: 3.601 ± 0.492
4.391AsnGlu: 4.391 ± 0.705
2.81AsnPhe: 2.81 ± 0.562
7.026AsnGly: 7.026 ± 1.26
1.142AsnHis: 1.142 ± 0.238
3.776AsnIle: 3.776 ± 0.54
4.83AsnLys: 4.83 ± 0.609
5.533AsnLeu: 5.533 ± 0.732
1.142AsnMet: 1.142 ± 0.33
4.742AsnAsn: 4.742 ± 0.731
2.986AsnPro: 2.986 ± 0.575
2.371AsnGln: 2.371 ± 0.386
2.02AsnArg: 2.02 ± 0.459
4.04AsnSer: 4.04 ± 0.589
3.425AsnThr: 3.425 ± 0.442
3.513AsnVal: 3.513 ± 0.463
1.317AsnTrp: 1.317 ± 0.248
1.844AsnTyr: 1.844 ± 0.408
0.0AsnXaa: 0.0 ± 0.0
Pro
1.669ProAla: 1.669 ± 0.324
0.088ProCys: 0.088 ± 0.077
1.493ProAsp: 1.493 ± 0.387
2.722ProGlu: 2.722 ± 0.53
1.142ProPhe: 1.142 ± 0.261
1.142ProGly: 1.142 ± 0.387
0.527ProHis: 0.527 ± 0.186
1.669ProIle: 1.669 ± 0.301
3.864ProLys: 3.864 ± 0.509
2.635ProLeu: 2.635 ± 0.483
0.176ProMet: 0.176 ± 0.118
2.459ProAsn: 2.459 ± 0.406
0.703ProPro: 0.703 ± 0.303
1.317ProGln: 1.317 ± 0.262
0.79ProArg: 0.79 ± 0.351
2.547ProSer: 2.547 ± 0.447
2.371ProThr: 2.371 ± 0.393
1.493ProVal: 1.493 ± 0.383
0.615ProTrp: 0.615 ± 0.194
1.142ProTyr: 1.142 ± 0.337
0.0ProXaa: 0.0 ± 0.0
Gln
3.864GlnAla: 3.864 ± 0.615
0.263GlnCys: 0.263 ± 0.141
1.932GlnAsp: 1.932 ± 0.359
2.459GlnGlu: 2.459 ± 0.527
1.581GlnPhe: 1.581 ± 0.403
3.161GlnGly: 3.161 ± 0.625
0.439GlnHis: 0.439 ± 0.191
2.195GlnIle: 2.195 ± 0.562
2.986GlnLys: 2.986 ± 0.534
3.249GlnLeu: 3.249 ± 0.457
1.229GlnMet: 1.229 ± 0.361
2.722GlnAsn: 2.722 ± 0.425
0.527GlnPro: 0.527 ± 0.213
2.459GlnGln: 2.459 ± 0.485
1.581GlnArg: 1.581 ± 0.376
2.371GlnSer: 2.371 ± 0.417
2.635GlnThr: 2.635 ± 0.574
1.932GlnVal: 1.932 ± 0.521
0.263GlnTrp: 0.263 ± 0.155
2.371GlnTyr: 2.371 ± 0.395
0.0GlnXaa: 0.0 ± 0.0
Arg
2.02ArgAla: 2.02 ± 0.464
0.527ArgCys: 0.527 ± 0.301
2.722ArgAsp: 2.722 ± 0.37
2.635ArgGlu: 2.635 ± 0.435
2.283ArgPhe: 2.283 ± 0.434
2.986ArgGly: 2.986 ± 0.516
0.878ArgHis: 0.878 ± 0.27
3.601ArgIle: 3.601 ± 0.577
2.81ArgLys: 2.81 ± 0.487
3.688ArgLeu: 3.688 ± 0.599
0.878ArgMet: 0.878 ± 0.304
3.074ArgAsn: 3.074 ± 0.464
1.317ArgPro: 1.317 ± 0.278
1.756ArgGln: 1.756 ± 0.393
1.142ArgArg: 1.142 ± 0.333
1.581ArgSer: 1.581 ± 0.339
2.81ArgThr: 2.81 ± 0.659
2.635ArgVal: 2.635 ± 0.365
1.142ArgTrp: 1.142 ± 0.308
2.635ArgTyr: 2.635 ± 0.508
0.0ArgXaa: 0.0 ± 0.0
Ser
3.601SerAla: 3.601 ± 0.639
0.527SerCys: 0.527 ± 0.253
4.479SerAsp: 4.479 ± 0.655
3.601SerGlu: 3.601 ± 0.409
2.986SerPhe: 2.986 ± 0.538
4.303SerGly: 4.303 ± 0.869
0.615SerHis: 0.615 ± 0.214
4.391SerIle: 4.391 ± 0.477
5.445SerLys: 5.445 ± 0.799
4.479SerLeu: 4.479 ± 0.584
1.142SerMet: 1.142 ± 0.26
4.654SerAsn: 4.654 ± 0.633
1.932SerPro: 1.932 ± 0.333
2.722SerGln: 2.722 ± 0.52
3.074SerArg: 3.074 ± 0.631
3.249SerSer: 3.249 ± 0.507
3.337SerThr: 3.337 ± 0.556
5.445SerVal: 5.445 ± 0.671
0.966SerTrp: 0.966 ± 0.345
1.493SerTyr: 1.493 ± 0.378
0.0SerXaa: 0.0 ± 0.0
Thr
4.303ThrAla: 4.303 ± 0.617
0.439ThrCys: 0.439 ± 0.182
4.04ThrAsp: 4.04 ± 0.577
3.513ThrGlu: 3.513 ± 0.458
3.249ThrPhe: 3.249 ± 0.734
3.425ThrGly: 3.425 ± 0.551
1.317ThrHis: 1.317 ± 0.273
4.479ThrIle: 4.479 ± 0.717
5.006ThrLys: 5.006 ± 0.605
6.06ThrLeu: 6.06 ± 0.681
0.966ThrMet: 0.966 ± 0.242
3.688ThrAsn: 3.688 ± 0.558
1.581ThrPro: 1.581 ± 0.434
2.547ThrGln: 2.547 ± 0.456
1.756ThrArg: 1.756 ± 0.331
3.776ThrSer: 3.776 ± 0.533
2.81ThrThr: 2.81 ± 0.626
3.776ThrVal: 3.776 ± 0.519
0.878ThrTrp: 0.878 ± 0.275
3.249ThrTyr: 3.249 ± 0.497
0.0ThrXaa: 0.0 ± 0.0
Val
4.215ValAla: 4.215 ± 0.566
0.263ValCys: 0.263 ± 0.141
5.269ValAsp: 5.269 ± 0.652
4.128ValGlu: 4.128 ± 0.62
3.074ValPhe: 3.074 ± 0.621
4.04ValGly: 4.04 ± 0.608
0.615ValHis: 0.615 ± 0.206
3.688ValIle: 3.688 ± 0.464
4.83ValLys: 4.83 ± 0.634
3.513ValLeu: 3.513 ± 0.49
0.966ValMet: 0.966 ± 0.275
3.601ValAsn: 3.601 ± 0.589
1.669ValPro: 1.669 ± 0.335
2.195ValGln: 2.195 ± 0.427
2.195ValArg: 2.195 ± 0.483
4.479ValSer: 4.479 ± 0.674
5.006ValThr: 5.006 ± 0.874
3.074ValVal: 3.074 ± 0.559
0.878ValTrp: 0.878 ± 0.23
1.405ValTyr: 1.405 ± 0.411
0.0ValXaa: 0.0 ± 0.0
Trp
0.527TrpAla: 0.527 ± 0.212
0.088TrpCys: 0.088 ± 0.081
0.878TrpAsp: 0.878 ± 0.353
1.054TrpGlu: 1.054 ± 0.222
0.79TrpPhe: 0.79 ± 0.239
0.703TrpGly: 0.703 ± 0.222
0.263TrpHis: 0.263 ± 0.133
0.439TrpIle: 0.439 ± 0.158
0.878TrpLys: 0.878 ± 0.278
1.317TrpLeu: 1.317 ± 0.339
0.263TrpMet: 0.263 ± 0.145
0.878TrpAsn: 0.878 ± 0.314
0.088TrpPro: 0.088 ± 0.083
0.966TrpGln: 0.966 ± 0.214
0.79TrpArg: 0.79 ± 0.23
1.844TrpSer: 1.844 ± 0.654
0.878TrpThr: 0.878 ± 0.258
1.493TrpVal: 1.493 ± 0.287
0.263TrpTrp: 0.263 ± 0.174
0.439TrpTyr: 0.439 ± 0.207
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.459TyrAla: 2.459 ± 0.327
0.703TyrCys: 0.703 ± 0.341
2.635TyrAsp: 2.635 ± 0.45
3.074TyrGlu: 3.074 ± 0.537
1.581TyrPhe: 1.581 ± 0.372
1.669TyrGly: 1.669 ± 0.375
0.703TyrHis: 0.703 ± 0.28
2.547TyrIle: 2.547 ± 0.41
3.513TyrLys: 3.513 ± 0.673
3.425TyrLeu: 3.425 ± 0.486
0.703TyrMet: 0.703 ± 0.228
1.844TyrAsn: 1.844 ± 0.372
1.405TyrPro: 1.405 ± 0.448
2.195TyrGln: 2.195 ± 0.38
2.547TyrArg: 2.547 ± 0.591
2.459TyrSer: 2.459 ± 0.512
1.844TyrThr: 1.844 ± 0.46
2.547TyrVal: 2.547 ± 0.485
0.263TyrTrp: 0.263 ± 0.141
2.195TyrTyr: 2.195 ± 0.625
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (11388 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski