Fusarium sp. AF-6

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium solani species complex; unclassified Fusarium solani species complex

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A428QLQ3|A0A428QLQ3_9HYPO Uncharacterized protein OS=Fusarium sp. AF-6 OX=1325737 GN=CEP53_003524 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84FTSVFVAGAFAAMASAATTTVGLSPAQQSQADD34 pKa = 4.0CLEE37 pKa = 4.19ACDD40 pKa = 5.12AGDD43 pKa = 3.84VKK45 pKa = 10.92CQSYY49 pKa = 10.65CITVPSPNEE58 pKa = 3.75KK59 pKa = 9.91QVNATTEE66 pKa = 4.2CVANCDD72 pKa = 3.78QGDD75 pKa = 3.83GTEE78 pKa = 4.32AQTEE82 pKa = 4.55KK83 pKa = 11.01YY84 pKa = 9.62AACLQEE90 pKa = 4.61CVSDD94 pKa = 4.38NYY96 pKa = 10.64WKK98 pKa = 9.62TIDD101 pKa = 3.49GTPRR105 pKa = 11.84EE106 pKa = 4.29TSASGSSSDD115 pKa = 4.82DD116 pKa = 4.78DD117 pKa = 4.11EE118 pKa = 7.17DD119 pKa = 4.95KK120 pKa = 11.27DD121 pKa = 4.22SSSKK125 pKa = 11.07ASAKK129 pKa = 9.67ATGTDD134 pKa = 3.35ADD136 pKa = 3.92STAAASASASSDD148 pKa = 3.28SDD150 pKa = 3.77DD151 pKa = 4.88DD152 pKa = 4.76ASEE155 pKa = 4.04TASDD159 pKa = 4.12SEE161 pKa = 4.57ASGTATGTSSSSSSTEE177 pKa = 3.8SGNAAPALVGGVSLLGLFAAVLALL201 pKa = 4.06

Molecular weight: 19.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A428P6L7|A0A428P6L7_9HYPO DUF2236 domain-containing protein OS=Fusarium sp. AF-6 OX=1325737 GN=CEP53_009452 PE=4 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.67RR8 pKa = 11.84HH9 pKa = 3.83TTPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APATRR27 pKa = 11.84SHH29 pKa = 6.26HH30 pKa = 4.94TTVTTTTTTRR40 pKa = 11.84KK41 pKa = 8.96PRR43 pKa = 11.84RR44 pKa = 11.84GLLGGGRR51 pKa = 11.84RR52 pKa = 11.84THH54 pKa = 7.04AAPVHH59 pKa = 5.1HH60 pKa = 5.69QQRR63 pKa = 11.84RR64 pKa = 11.84PSMKK68 pKa = 10.24DD69 pKa = 2.95KK70 pKa = 11.41VSGALLKK77 pKa = 11.04LKK79 pKa = 10.68GSLTRR84 pKa = 11.84RR85 pKa = 11.84PGVKK89 pKa = 9.89AAGTRR94 pKa = 11.84RR95 pKa = 11.84MRR97 pKa = 11.84GTDD100 pKa = 2.74GRR102 pKa = 11.84GSHH105 pKa = 5.81RR106 pKa = 11.84RR107 pKa = 11.84ARR109 pKa = 11.84HH110 pKa = 4.06FF111 pKa = 3.77

Molecular weight: 12.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15273

0

15273

7097187

50

7829

464.7

51.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.455 ± 0.02

1.315 ± 0.008

5.882 ± 0.016

6.308 ± 0.024

3.82 ± 0.013

6.914 ± 0.023

2.358 ± 0.01

4.997 ± 0.016

4.867 ± 0.018

8.934 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.008

3.604 ± 0.009

5.985 ± 0.025

3.915 ± 0.015

5.936 ± 0.019

7.94 ± 0.025

5.968 ± 0.024

6.205 ± 0.014

1.6 ± 0.008

2.757 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski