Apis mellifera associated microvirus 29

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U516|A0A3Q8U516_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 29 OX=2494757 PE=3 SV=1
MM1 pKa = 7.74KK2 pKa = 8.93FTNHH6 pKa = 4.73IQEE9 pKa = 3.74TRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84VTIDD17 pKa = 3.05CSEE20 pKa = 4.19PQVTDD25 pKa = 3.29QSFKK29 pKa = 10.91KK30 pKa = 10.63AVDD33 pKa = 3.14INNIMKK39 pKa = 10.14QYY41 pKa = 10.02EE42 pKa = 4.32KK43 pKa = 10.5TGVLPSGNNRR53 pKa = 11.84QPQFIDD59 pKa = 3.17TTLIPSLEE67 pKa = 3.96EE68 pKa = 3.56SFNIVNQAYY77 pKa = 10.29DD78 pKa = 3.93LFYY81 pKa = 10.73QLPATIRR88 pKa = 11.84KK89 pKa = 9.44LMDD92 pKa = 3.68NDD94 pKa = 3.54ASKK97 pKa = 11.55LEE99 pKa = 4.49AFLSDD104 pKa = 3.76PQNHH108 pKa = 5.79EE109 pKa = 4.03LCLQYY114 pKa = 11.72GLITKK119 pKa = 8.56PQEE122 pKa = 3.92TTPTEE127 pKa = 4.48GVKK130 pKa = 9.03STPGIIPSEE139 pKa = 3.95NTQQNN144 pKa = 3.35

Molecular weight: 16.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UU61|A0A3S8UU61_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 29 OX=2494757 PE=4 SV=1
MM1 pKa = 7.42SLATGLSLVDD11 pKa = 3.37TAANIWSARR20 pKa = 11.84NANKK24 pKa = 9.85QNQAMARR31 pKa = 11.84EE32 pKa = 3.94QMAFQEE38 pKa = 4.4RR39 pKa = 11.84MSNTAHH45 pKa = 5.48QRR47 pKa = 11.84EE48 pKa = 4.41VADD51 pKa = 4.87LKK53 pKa = 11.28AAGLNPILSAGGSGASSPGGASASVTPEE81 pKa = 3.81VQGSPLSTALARR93 pKa = 11.84HH94 pKa = 5.94NEE96 pKa = 3.88NATAKK101 pKa = 10.06QALNNAKK108 pKa = 10.21AQEE111 pKa = 4.33TLLGTQNEE119 pKa = 4.39KK120 pKa = 10.27TKK122 pKa = 10.89VDD124 pKa = 5.03AINSAADD131 pKa = 3.54TRR133 pKa = 11.84KK134 pKa = 10.45KK135 pKa = 9.65MAEE138 pKa = 4.01AKK140 pKa = 10.54NADD143 pKa = 3.91TLNLKK148 pKa = 10.31LSQDD152 pKa = 3.38IQNGAIQQQMLKK164 pKa = 10.48LALPKK169 pKa = 10.37LLNEE173 pKa = 4.21AQFEE177 pKa = 4.26KK178 pKa = 10.6DD179 pKa = 2.96YY180 pKa = 11.51GAAKK184 pKa = 10.07RR185 pKa = 11.84KK186 pKa = 9.89SDD188 pKa = 3.86ALLDD192 pKa = 3.92TVNKK196 pKa = 9.57GLHH199 pKa = 6.25GANQVKK205 pKa = 10.41NLFNPLRR212 pKa = 11.84GIFGGSNTEE221 pKa = 4.04TVIDD225 pKa = 4.37GNTGEE230 pKa = 4.96ILDD233 pKa = 4.17QIRR236 pKa = 11.84KK237 pKa = 8.51KK238 pKa = 10.22HH239 pKa = 5.12

Molecular weight: 25.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1309

80

545

261.8

29.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.327 ± 1.772

0.84 ± 0.359

5.042 ± 0.182

5.653 ± 0.926

4.736 ± 0.896

6.57 ± 0.967

2.674 ± 0.599

5.73 ± 0.51

6.188 ± 1.595

8.403 ± 0.634

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.833 ± 0.218

6.57 ± 0.744

5.195 ± 0.728

5.042 ± 0.983

4.66 ± 0.552

6.723 ± 0.241

7.563 ± 0.596

3.82 ± 0.478

0.764 ± 0.265

3.667 ± 0.925
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski