Streptococcus phage Javan102
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6APL2|A0A4D6APL2_9CAUD DUF4314 domain-containing protein OS=Streptococcus phage Javan102 OX=2547975 GN=Javan102_0030 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 5.45 KK3 pKa = 10.69 LLEE6 pKa = 4.69 IIEE9 pKa = 4.72 KK10 pKa = 10.26 IGFPSAYY17 pKa = 9.15 HH18 pKa = 6.19 HH19 pKa = 6.06 FAEE22 pKa = 5.44 GEE24 pKa = 4.4 SPNPPFLIYY33 pKa = 10.31 ILPASDD39 pKa = 3.92 NFSADD44 pKa = 2.46 GRR46 pKa = 11.84 VYY48 pKa = 9.99 FKK50 pKa = 11.14 ANEE53 pKa = 3.89 VHH55 pKa = 6.62 IEE57 pKa = 3.99 VYY59 pKa = 10.1 TDD61 pKa = 3.34 YY62 pKa = 11.6 KK63 pKa = 11.26 NLDD66 pKa = 3.22 IEE68 pKa = 4.55 KK69 pKa = 9.87 KK70 pKa = 10.05 VEE72 pKa = 4.13 VVLDD76 pKa = 4.03 EE77 pKa = 4.57 YY78 pKa = 11.46 GIFYY82 pKa = 10.57 NKK84 pKa = 10.27 SEE86 pKa = 4.22 VYY88 pKa = 10.04 IEE90 pKa = 4.13 SEE92 pKa = 3.7 KK93 pKa = 10.87 LYY95 pKa = 9.42 EE96 pKa = 3.81 VLYY99 pKa = 10.08 IFEE102 pKa = 4.88 MEE104 pKa = 4.16 VKK106 pKa = 9.97 TNGKK110 pKa = 9.41
Molecular weight: 12.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.525
IPC2_protein 4.685
IPC_protein 4.558
Toseland 4.444
ProMoST 4.66
Dawson 4.495
Bjellqvist 4.647
Wikipedia 4.329
Rodwell 4.418
Grimsley 4.355
Solomon 4.495
Lehninger 4.444
Nozaki 4.609
DTASelect 4.698
Thurlkill 4.431
EMBOSS 4.355
Sillero 4.673
Patrickios 3.198
IPC_peptide 4.507
IPC2_peptide 4.673
IPC2.peptide.svr19 4.622
Protein with the highest isoelectric point:
>tr|A0A4D6AP42|A0A4D6AP42_9CAUD DNA polymerase I OS=Streptococcus phage Javan102 OX=2547975 GN=Javan102_0046 PE=4 SV=1
MM1 pKa = 7.46 WKK3 pKa = 9.89 RR4 pKa = 11.84 VRR6 pKa = 11.84 DD7 pKa = 4.08 AYY9 pKa = 11.0 VKK11 pKa = 8.9 EE12 pKa = 4.28 HH13 pKa = 6.85 PFCEE17 pKa = 4.09 EE18 pKa = 3.87 CFKK21 pKa = 11.23 KK22 pKa = 10.57 KK23 pKa = 10.2 ILVPVEE29 pKa = 4.23 EE30 pKa = 4.27 VHH32 pKa = 7.14 HH33 pKa = 6.28 IKK35 pKa = 10.36 PLSEE39 pKa = 4.2 GGNHH43 pKa = 6.06 NKK45 pKa = 10.23 SNLISLCKK53 pKa = 9.56 SCHH56 pKa = 5.11 ARR58 pKa = 11.84 IHH60 pKa = 6.45 ASRR63 pKa = 11.84 GDD65 pKa = 3.07 RR66 pKa = 11.84 WNKK69 pKa = 9.96 NKK71 pKa = 10.47 GG72 pKa = 3.27
Molecular weight: 8.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.853
IPC2_protein 8.741
IPC_protein 8.682
Toseland 9.706
ProMoST 9.253
Dawson 9.823
Bjellqvist 9.545
Wikipedia 9.897
Rodwell 10.482
Grimsley 9.823
Solomon 9.911
Lehninger 9.911
Nozaki 9.911
DTASelect 9.458
Thurlkill 9.736
EMBOSS 10.087
Sillero 9.823
Patrickios 10.35
IPC_peptide 9.911
IPC2_peptide 8.331
IPC2.peptide.svr19 7.737
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
65
0
65
16606
38
941
255.5
29.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.191 ± 0.371
1.186 ± 0.115
6.185 ± 0.208
8.148 ± 0.29
3.968 ± 0.171
6.257 ± 0.297
1.626 ± 0.115
8.033 ± 0.315
8.924 ± 0.26
8.328 ± 0.24
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.632 ± 0.137
5.552 ± 0.209
2.987 ± 0.2
2.939 ± 0.147
4.239 ± 0.261
6.51 ± 0.283
5.149 ± 0.206
5.811 ± 0.203
1.168 ± 0.071
4.167 ± 0.284
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here