Corynebacterium jeikeium (strain K411)
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2064 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4JSY0|Q4JSY0_CORJK Putative cation-transporting P-type ATPase OS=Corynebacterium jeikeium (strain K411) OX=306537 GN=ctpA2 PE=4 SV=1
MM1 pKa = 7.54 SVFQCPVCDD10 pKa = 3.62 YY11 pKa = 10.99 RR12 pKa = 11.84 FDD14 pKa = 3.9 EE15 pKa = 4.72 AVGDD19 pKa = 3.87 AAEE22 pKa = 4.72 GFDD25 pKa = 5.26 PGTSWSEE32 pKa = 4.04 VPDD35 pKa = 3.81 DD36 pKa = 4.71 WFCPDD41 pKa = 3.5 CGVRR45 pKa = 11.84 DD46 pKa = 3.76 KK47 pKa = 11.82 VDD49 pKa = 4.24 FEE51 pKa = 4.52 PVAA54 pKa = 4.3
Molecular weight: 6.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.803
IPC2_protein 3.579
IPC_protein 3.516
Toseland 3.312
ProMoST 3.732
Dawson 3.541
Bjellqvist 3.706
Wikipedia 3.528
Rodwell 3.363
Grimsley 3.236
Solomon 3.49
Lehninger 3.452
Nozaki 3.681
DTASelect 3.897
Thurlkill 3.401
EMBOSS 3.528
Sillero 3.643
Patrickios 0.604
IPC_peptide 3.49
IPC2_peptide 3.605
IPC2.peptide.svr19 3.669
Protein with the highest isoelectric point:
>tr|Q4JSD0|Q4JSD0_CORJK Putative hydrolase OS=Corynebacterium jeikeium (strain K411) OX=306537 GN=cwlM PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 NKK40 pKa = 9.24 GRR42 pKa = 11.84 KK43 pKa = 9.07 SLTAA47 pKa = 4.07
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2064
0
2064
716252
24
3618
347.0
37.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.473 ± 0.069
0.704 ± 0.013
6.055 ± 0.045
6.611 ± 0.055
3.19 ± 0.03
8.589 ± 0.048
2.074 ± 0.023
4.755 ± 0.036
3.778 ± 0.046
9.226 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.248 ± 0.022
3.1 ± 0.029
5.062 ± 0.036
3.559 ± 0.031
6.197 ± 0.049
5.919 ± 0.036
5.88 ± 0.034
8.033 ± 0.05
1.395 ± 0.021
2.152 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here