Pseudomonas phage PaBG

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Baikalvirus; Pseudomonas virus PaBG

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 308 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5WBB7|S5WBB7_9CAUD Uncharacterized protein OS=Pseudomonas phage PaBG OX=1335230 GN=PaBG_00179 PE=4 SV=1
MM1 pKa = 6.9NTVLLLGLAVFLFFWLTVIWSRR23 pKa = 11.84FVTDD27 pKa = 3.39VYY29 pKa = 11.52LNVAQAAIDD38 pKa = 3.7ASRR41 pKa = 11.84CCMLFDD47 pKa = 5.47QITAGDD53 pKa = 3.65WDD55 pKa = 4.51FSTVCNSCEE64 pKa = 3.61FDD66 pKa = 3.89LPFVDD71 pKa = 6.72FASQPISWWPAVWVWDD87 pKa = 4.75FIGRR91 pKa = 11.84QIVMEE96 pKa = 4.37HH97 pKa = 6.19SASNMEE103 pKa = 4.47LDD105 pKa = 3.22WAGVEE110 pKa = 4.56FSVV113 pKa = 5.14

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5VZL8|S5VZL8_9CAUD DNA topoisomerase (ATP-hydrolyzing) OS=Pseudomonas phage PaBG OX=1335230 GN=PaBG_00146 PE=4 SV=1
MM1 pKa = 7.5SRR3 pKa = 11.84TKK5 pKa = 10.44HH6 pKa = 5.69GSKK9 pKa = 10.29QPGEE13 pKa = 4.06FWSRR17 pKa = 11.84RR18 pKa = 11.84PFSSIGYY25 pKa = 8.77GRR27 pKa = 11.84EE28 pKa = 3.88LKK30 pKa = 9.23TLCHH34 pKa = 5.83RR35 pKa = 11.84AEE37 pKa = 3.8RR38 pKa = 11.84RR39 pKa = 11.84IADD42 pKa = 3.92RR43 pKa = 11.84EE44 pKa = 3.97IQRR47 pKa = 11.84EE48 pKa = 4.07LRR50 pKa = 11.84EE51 pKa = 4.04LL52 pKa = 3.66

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

308

0

308

77168

52

1969

250.5

27.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.139 ± 0.195

1.039 ± 0.055

6.288 ± 0.154

6.31 ± 0.149

3.952 ± 0.095

6.689 ± 0.185

2.234 ± 0.089

5.147 ± 0.103

6.135 ± 0.26

8.447 ± 0.151

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.073

4.21 ± 0.104

4.366 ± 0.1

3.879 ± 0.091

5.813 ± 0.122

5.949 ± 0.112

5.843 ± 0.195

7.471 ± 0.129

1.216 ± 0.057

3.338 ± 0.082
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski