Amino acid dipepetide frequency for Pseudomonas phage PaBG

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.058AlaAla: 9.058 ± 0.547
0.79AlaCys: 0.79 ± 0.099
5.961AlaAsp: 5.961 ± 0.257
5.689AlaGlu: 5.689 ± 0.302
3.641AlaPhe: 3.641 ± 0.211
6.143AlaGly: 6.143 ± 0.393
2.009AlaHis: 2.009 ± 0.16
4.704AlaIle: 4.704 ± 0.25
6.259AlaLys: 6.259 ± 0.542
7.853AlaLeu: 7.853 ± 0.353
2.682AlaMet: 2.682 ± 0.213
4.095AlaAsn: 4.095 ± 0.288
3.317AlaPro: 3.317 ± 0.195
3.693AlaGln: 3.693 ± 0.233
4.769AlaArg: 4.769 ± 0.263
5.274AlaSer: 5.274 ± 0.381
5.184AlaThr: 5.184 ± 0.39
6.505AlaVal: 6.505 ± 0.29
0.907AlaTrp: 0.907 ± 0.117
2.851AlaTyr: 2.851 ± 0.212
0.0AlaXaa: 0.0 ± 0.0
Cys
1.153CysAla: 1.153 ± 0.151
0.168CysCys: 0.168 ± 0.046
0.596CysAsp: 0.596 ± 0.091
0.739CysGlu: 0.739 ± 0.103
0.441CysPhe: 0.441 ± 0.078
0.531CysGly: 0.531 ± 0.096
0.35CysHis: 0.35 ± 0.066
0.441CysIle: 0.441 ± 0.068
0.505CysLys: 0.505 ± 0.089
0.778CysLeu: 0.778 ± 0.112
0.272CysMet: 0.272 ± 0.053
0.35CysAsn: 0.35 ± 0.067
0.635CysPro: 0.635 ± 0.084
0.298CysGln: 0.298 ± 0.062
0.661CysArg: 0.661 ± 0.101
0.609CysSer: 0.609 ± 0.087
0.57CysThr: 0.57 ± 0.085
0.829CysVal: 0.829 ± 0.096
0.168CysTrp: 0.168 ± 0.054
0.298CysTyr: 0.298 ± 0.063
0.0CysXaa: 0.0 ± 0.0
Asp
5.65AspAla: 5.65 ± 0.309
0.531AspCys: 0.531 ± 0.085
4.406AspAsp: 4.406 ± 0.563
5.132AspGlu: 5.132 ± 0.548
2.488AspPhe: 2.488 ± 0.199
5.171AspGly: 5.171 ± 0.292
1.257AspHis: 1.257 ± 0.138
3.162AspIle: 3.162 ± 0.183
3.667AspLys: 3.667 ± 0.299
5.041AspLeu: 5.041 ± 0.257
1.711AspMet: 1.711 ± 0.138
2.294AspAsn: 2.294 ± 0.182
3.019AspPro: 3.019 ± 0.247
2.048AspGln: 2.048 ± 0.141
2.89AspArg: 2.89 ± 0.174
3.058AspSer: 3.058 ± 0.208
3.175AspThr: 3.175 ± 0.202
5.313AspVal: 5.313 ± 0.256
0.894AspTrp: 0.894 ± 0.122
1.97AspTyr: 1.97 ± 0.171
0.0AspXaa: 0.0 ± 0.0
Glu
5.832GluAla: 5.832 ± 0.349
0.726GluCys: 0.726 ± 0.107
4.549GluAsp: 4.549 ± 0.462
4.587GluGlu: 4.587 ± 0.531
3.006GluPhe: 3.006 ± 0.193
3.875GluGly: 3.875 ± 0.225
1.607GluHis: 1.607 ± 0.155
3.253GluIle: 3.253 ± 0.232
3.421GluLys: 3.421 ± 0.287
6.531GluLeu: 6.531 ± 0.302
1.205GluMet: 1.205 ± 0.127
2.346GluAsn: 2.346 ± 0.159
2.125GluPro: 2.125 ± 0.168
2.838GluGln: 2.838 ± 0.211
3.784GluArg: 3.784 ± 0.223
3.097GluSer: 3.097 ± 0.214
2.942GluThr: 2.942 ± 0.203
4.6GluVal: 4.6 ± 0.251
0.557GluTrp: 0.557 ± 0.093
2.216GluTyr: 2.216 ± 0.204
0.0GluXaa: 0.0 ± 0.0
Phe
3.525PheAla: 3.525 ± 0.225
0.492PheCys: 0.492 ± 0.087
3.097PheAsp: 3.097 ± 0.167
2.67PheGlu: 2.67 ± 0.18
1.49PhePhe: 1.49 ± 0.163
2.605PheGly: 2.605 ± 0.202
0.726PheHis: 0.726 ± 0.111
2.086PheIle: 2.086 ± 0.151
2.371PheLys: 2.371 ± 0.195
2.553PheLeu: 2.553 ± 0.194
0.881PheMet: 0.881 ± 0.108
1.879PheAsn: 1.879 ± 0.13
1.464PhePro: 1.464 ± 0.153
1.322PheGln: 1.322 ± 0.123
2.32PheArg: 2.32 ± 0.167
2.384PheSer: 2.384 ± 0.222
2.825PheThr: 2.825 ± 0.241
2.981PheVal: 2.981 ± 0.224
0.479PheTrp: 0.479 ± 0.069
1.374PheTyr: 1.374 ± 0.159
0.0PheXaa: 0.0 ± 0.0
Gly
5.598GlyAla: 5.598 ± 0.364
0.829GlyCys: 0.829 ± 0.108
4.51GlyAsp: 4.51 ± 0.23
4.315GlyGlu: 4.315 ± 0.23
2.553GlyPhe: 2.553 ± 0.189
4.756GlyGly: 4.756 ± 0.452
1.464GlyHis: 1.464 ± 0.127
3.33GlyIle: 3.33 ± 0.223
4.73GlyLys: 4.73 ± 0.309
4.665GlyLeu: 4.665 ± 0.268
1.957GlyMet: 1.957 ± 0.158
3.24GlyAsn: 3.24 ± 0.285
2.682GlyPro: 2.682 ± 0.347
2.333GlyGln: 2.333 ± 0.167
3.706GlyArg: 3.706 ± 0.24
3.901GlySer: 3.901 ± 0.323
3.81GlyThr: 3.81 ± 0.382
5.365GlyVal: 5.365 ± 0.282
0.778GlyTrp: 0.778 ± 0.124
2.371GlyTyr: 2.371 ± 0.174
0.0GlyXaa: 0.0 ± 0.0
His
1.892HisAla: 1.892 ± 0.177
0.376HisCys: 0.376 ± 0.079
1.387HisAsp: 1.387 ± 0.153
1.27HisGlu: 1.27 ± 0.14
0.842HisPhe: 0.842 ± 0.105
1.555HisGly: 1.555 ± 0.131
0.518HisHis: 0.518 ± 0.086
1.568HisIle: 1.568 ± 0.136
1.153HisLys: 1.153 ± 0.127
1.724HisLeu: 1.724 ± 0.178
0.583HisMet: 0.583 ± 0.083
0.726HisAsn: 0.726 ± 0.092
1.14HisPro: 1.14 ± 0.129
0.894HisGln: 0.894 ± 0.089
1.244HisArg: 1.244 ± 0.143
1.205HisSer: 1.205 ± 0.137
1.335HisThr: 1.335 ± 0.137
1.814HisVal: 1.814 ± 0.167
0.298HisTrp: 0.298 ± 0.057
0.816HisTyr: 0.816 ± 0.105
0.0HisXaa: 0.0 ± 0.0
Ile
5.184IleAla: 5.184 ± 0.27
0.415IleCys: 0.415 ± 0.068
3.965IleAsp: 3.965 ± 0.213
3.823IleGlu: 3.823 ± 0.203
1.348IlePhe: 1.348 ± 0.147
3.266IleGly: 3.266 ± 0.217
0.946IleHis: 0.946 ± 0.114
2.695IleIle: 2.695 ± 0.174
3.382IleLys: 3.382 ± 0.241
3.395IleLeu: 3.395 ± 0.194
1.127IleMet: 1.127 ± 0.139
2.566IleAsn: 2.566 ± 0.203
2.514IlePro: 2.514 ± 0.187
1.879IleGln: 1.879 ± 0.136
2.916IleArg: 2.916 ± 0.171
2.786IleSer: 2.786 ± 0.232
3.356IleThr: 3.356 ± 0.22
4.056IleVal: 4.056 ± 0.235
0.505IleTrp: 0.505 ± 0.075
1.335IleTyr: 1.335 ± 0.144
0.0IleXaa: 0.0 ± 0.0
Lys
6.609LysAla: 6.609 ± 0.58
0.505LysCys: 0.505 ± 0.083
2.916LysAsp: 2.916 ± 0.253
3.434LysGlu: 3.434 ± 0.249
2.294LysPhe: 2.294 ± 0.169
4.03LysGly: 4.03 ± 0.301
1.607LysHis: 1.607 ± 0.176
2.682LysIle: 2.682 ± 0.212
5.3LysLys: 5.3 ± 0.663
5.832LysLeu: 5.832 ± 0.35
1.361LysMet: 1.361 ± 0.158
2.035LysAsn: 2.035 ± 0.157
3.084LysPro: 3.084 ± 0.264
2.462LysGln: 2.462 ± 0.205
3.849LysArg: 3.849 ± 0.307
3.888LysSer: 3.888 ± 0.34
3.097LysThr: 3.097 ± 0.193
3.667LysVal: 3.667 ± 0.271
0.79LysTrp: 0.79 ± 0.114
1.905LysTyr: 1.905 ± 0.16
0.0LysXaa: 0.0 ± 0.0
Leu
8.073LeuAla: 8.073 ± 0.347
0.713LeuCys: 0.713 ± 0.112
5.248LeuAsp: 5.248 ± 0.272
5.171LeuGlu: 5.171 ± 0.282
3.201LeuPhe: 3.201 ± 0.206
5.106LeuGly: 5.106 ± 0.269
2.022LeuHis: 2.022 ± 0.172
3.797LeuIle: 3.797 ± 0.21
4.691LeuLys: 4.691 ± 0.328
6.609LeuLeu: 6.609 ± 0.355
2.099LeuMet: 2.099 ± 0.18
3.94LeuAsn: 3.94 ± 0.231
4.289LeuPro: 4.289 ± 0.233
3.382LeuGln: 3.382 ± 0.233
5.741LeuArg: 5.741 ± 0.281
5.313LeuSer: 5.313 ± 0.261
4.847LeuThr: 4.847 ± 0.247
6.428LeuVal: 6.428 ± 0.299
0.894LeuTrp: 0.894 ± 0.125
2.903LeuTyr: 2.903 ± 0.186
0.0LeuXaa: 0.0 ± 0.0
Met
2.527MetAla: 2.527 ± 0.206
0.246MetCys: 0.246 ± 0.061
1.335MetAsp: 1.335 ± 0.136
1.425MetGlu: 1.425 ± 0.141
0.946MetPhe: 0.946 ± 0.115
1.477MetGly: 1.477 ± 0.133
0.57MetHis: 0.57 ± 0.091
1.05MetIle: 1.05 ± 0.121
1.4MetLys: 1.4 ± 0.164
2.384MetLeu: 2.384 ± 0.166
0.622MetMet: 0.622 ± 0.086
1.114MetAsn: 1.114 ± 0.121
1.438MetPro: 1.438 ± 0.139
1.076MetGln: 1.076 ± 0.121
1.827MetArg: 1.827 ± 0.151
1.944MetSer: 1.944 ± 0.149
1.309MetThr: 1.309 ± 0.116
1.516MetVal: 1.516 ± 0.138
0.337MetTrp: 0.337 ± 0.062
0.816MetTyr: 0.816 ± 0.108
0.0MetXaa: 0.0 ± 0.0
Asn
3.616AsnAla: 3.616 ± 0.26
0.428AsnCys: 0.428 ± 0.07
2.397AsnAsp: 2.397 ± 0.173
2.164AsnGlu: 2.164 ± 0.214
1.801AsnPhe: 1.801 ± 0.165
3.862AsnGly: 3.862 ± 0.293
0.739AsnHis: 0.739 ± 0.105
2.384AsnIle: 2.384 ± 0.23
2.164AsnLys: 2.164 ± 0.197
3.421AsnLeu: 3.421 ± 0.228
1.192AsnMet: 1.192 ± 0.118
1.724AsnAsn: 1.724 ± 0.171
2.229AsnPro: 2.229 ± 0.202
1.555AsnGln: 1.555 ± 0.153
2.371AsnArg: 2.371 ± 0.172
2.371AsnSer: 2.371 ± 0.179
2.475AsnThr: 2.475 ± 0.214
3.214AsnVal: 3.214 ± 0.204
0.674AsnTrp: 0.674 ± 0.105
1.322AsnTyr: 1.322 ± 0.139
0.0AsnXaa: 0.0 ± 0.0
Pro
3.745ProAla: 3.745 ± 0.242
0.376ProCys: 0.376 ± 0.079
2.89ProAsp: 2.89 ± 0.243
2.812ProGlu: 2.812 ± 0.208
1.931ProPhe: 1.931 ± 0.157
2.307ProGly: 2.307 ± 0.248
0.972ProHis: 0.972 ± 0.115
2.734ProIle: 2.734 ± 0.2
3.356ProLys: 3.356 ± 0.254
3.266ProLeu: 3.266 ± 0.233
1.153ProMet: 1.153 ± 0.115
1.827ProAsn: 1.827 ± 0.15
1.361ProPro: 1.361 ± 0.167
1.503ProGln: 1.503 ± 0.14
2.346ProArg: 2.346 ± 0.158
2.786ProSer: 2.786 ± 0.144
3.11ProThr: 3.11 ± 0.191
3.071ProVal: 3.071 ± 0.206
0.583ProTrp: 0.583 ± 0.091
1.529ProTyr: 1.529 ± 0.14
0.0ProXaa: 0.0 ± 0.0
Gln
3.369GlnAla: 3.369 ± 0.257
0.415GlnCys: 0.415 ± 0.079
1.711GlnAsp: 1.711 ± 0.137
2.203GlnGlu: 2.203 ± 0.191
1.827GlnPhe: 1.827 ± 0.164
2.423GlnGly: 2.423 ± 0.204
0.946GlnHis: 0.946 ± 0.094
2.203GlnIle: 2.203 ± 0.204
1.983GlnLys: 1.983 ± 0.2
3.952GlnLeu: 3.952 ± 0.222
1.153GlnMet: 1.153 ± 0.119
1.322GlnAsn: 1.322 ± 0.122
1.892GlnPro: 1.892 ± 0.15
1.801GlnGln: 1.801 ± 0.171
2.553GlnArg: 2.553 ± 0.178
1.996GlnSer: 1.996 ± 0.163
2.009GlnThr: 2.009 ± 0.195
2.799GlnVal: 2.799 ± 0.173
0.635GlnTrp: 0.635 ± 0.118
1.594GlnTyr: 1.594 ± 0.13
0.0GlnXaa: 0.0 ± 0.0
Arg
4.821ArgAla: 4.821 ± 0.234
0.674ArgCys: 0.674 ± 0.095
3.447ArgAsp: 3.447 ± 0.236
3.978ArgGlu: 3.978 ± 0.238
2.086ArgPhe: 2.086 ± 0.167
3.616ArgGly: 3.616 ± 0.227
1.257ArgHis: 1.257 ± 0.138
3.577ArgIle: 3.577 ± 0.198
3.641ArgLys: 3.641 ± 0.247
5.274ArgLeu: 5.274 ± 0.267
1.62ArgMet: 1.62 ± 0.149
2.838ArgAsn: 2.838 ± 0.169
2.073ArgPro: 2.073 ± 0.162
2.255ArgGln: 2.255 ± 0.175
3.499ArgArg: 3.499 ± 0.249
3.499ArgSer: 3.499 ± 0.181
3.214ArgThr: 3.214 ± 0.21
3.965ArgVal: 3.965 ± 0.234
0.726ArgTrp: 0.726 ± 0.102
2.073ArgTyr: 2.073 ± 0.146
0.0ArgXaa: 0.0 ± 0.0
Ser
5.197SerAla: 5.197 ± 0.378
0.557SerCys: 0.557 ± 0.074
3.94SerAsp: 3.94 ± 0.253
3.292SerGlu: 3.292 ± 0.238
2.32SerPhe: 2.32 ± 0.182
4.665SerGly: 4.665 ± 0.312
1.179SerHis: 1.179 ± 0.114
3.11SerIle: 3.11 ± 0.198
3.473SerLys: 3.473 ± 0.313
5.145SerLeu: 5.145 ± 0.288
1.464SerMet: 1.464 ± 0.158
2.812SerAsn: 2.812 ± 0.206
2.268SerPro: 2.268 ± 0.201
2.255SerGln: 2.255 ± 0.164
2.916SerArg: 2.916 ± 0.168
4.017SerSer: 4.017 ± 0.366
3.693SerThr: 3.693 ± 0.229
4.626SerVal: 4.626 ± 0.251
0.739SerTrp: 0.739 ± 0.098
1.827SerTyr: 1.827 ± 0.173
0.0SerXaa: 0.0 ± 0.0
Thr
5.222ThrAla: 5.222 ± 0.381
0.557ThrCys: 0.557 ± 0.084
3.071ThrAsp: 3.071 ± 0.168
2.955ThrGlu: 2.955 ± 0.204
2.695ThrPhe: 2.695 ± 0.252
4.354ThrGly: 4.354 ± 0.342
1.477ThrHis: 1.477 ± 0.139
3.162ThrIle: 3.162 ± 0.264
3.032ThrLys: 3.032 ± 0.196
5.495ThrLeu: 5.495 ± 0.306
1.49ThrMet: 1.49 ± 0.148
2.138ThrAsn: 2.138 ± 0.155
2.89ThrPro: 2.89 ± 0.21
2.268ThrGln: 2.268 ± 0.215
2.942ThrArg: 2.942 ± 0.2
3.421ThrSer: 3.421 ± 0.262
3.46ThrThr: 3.46 ± 0.368
5.132ThrVal: 5.132 ± 0.364
0.7ThrTrp: 0.7 ± 0.097
1.97ThrTyr: 1.97 ± 0.188
0.0ThrXaa: 0.0 ± 0.0
Val
6.259ValAla: 6.259 ± 0.291
0.92ValCys: 0.92 ± 0.124
4.38ValAsp: 4.38 ± 0.242
4.886ValGlu: 4.886 ± 0.26
2.812ValPhe: 2.812 ± 0.191
4.121ValGly: 4.121 ± 0.264
1.698ValHis: 1.698 ± 0.177
3.486ValIle: 3.486 ± 0.204
4.678ValLys: 4.678 ± 0.298
6.544ValLeu: 6.544 ± 0.301
1.659ValMet: 1.659 ± 0.126
3.084ValAsn: 3.084 ± 0.222
3.551ValPro: 3.551 ± 0.266
2.89ValGln: 2.89 ± 0.196
4.717ValArg: 4.717 ± 0.254
4.911ValSer: 4.911 ± 0.248
4.976ValThr: 4.976 ± 0.477
5.508ValVal: 5.508 ± 0.297
0.998ValTrp: 0.998 ± 0.121
2.631ValTyr: 2.631 ± 0.216
0.0ValXaa: 0.0 ± 0.0
Trp
0.881TrpAla: 0.881 ± 0.104
0.246TrpCys: 0.246 ± 0.052
0.79TrpAsp: 0.79 ± 0.103
0.778TrpGlu: 0.778 ± 0.119
0.454TrpPhe: 0.454 ± 0.074
0.7TrpGly: 0.7 ± 0.104
0.311TrpHis: 0.311 ± 0.056
0.518TrpIle: 0.518 ± 0.083
0.609TrpLys: 0.609 ± 0.082
1.114TrpLeu: 1.114 ± 0.122
0.298TrpMet: 0.298 ± 0.065
0.57TrpAsn: 0.57 ± 0.106
0.467TrpPro: 0.467 ± 0.081
0.518TrpGln: 0.518 ± 0.074
0.868TrpArg: 0.868 ± 0.124
0.959TrpSer: 0.959 ± 0.11
0.557TrpThr: 0.557 ± 0.091
0.907TrpVal: 0.907 ± 0.1
0.104TrpTrp: 0.104 ± 0.037
0.505TrpTyr: 0.505 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.175TyrAla: 3.175 ± 0.226
0.428TyrCys: 0.428 ± 0.081
2.281TyrAsp: 2.281 ± 0.16
1.775TyrGlu: 1.775 ± 0.16
1.348TyrPhe: 1.348 ± 0.126
2.333TyrGly: 2.333 ± 0.173
0.687TyrHis: 0.687 ± 0.105
1.529TyrIle: 1.529 ± 0.128
1.529TyrLys: 1.529 ± 0.159
2.89TyrLeu: 2.89 ± 0.183
0.816TyrMet: 0.816 ± 0.116
1.205TyrAsn: 1.205 ± 0.141
1.218TyrPro: 1.218 ± 0.168
1.516TyrGln: 1.516 ± 0.129
2.216TyrArg: 2.216 ± 0.153
2.073TyrSer: 2.073 ± 0.169
2.488TyrThr: 2.488 ± 0.218
2.41TyrVal: 2.41 ± 0.203
0.389TyrTrp: 0.389 ± 0.074
1.076TyrTyr: 1.076 ± 0.136
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 308 proteins (77168 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski